• 설비공학논문집
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• 제7권4호
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• pp.681-693
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• 1995

A new set of capillary tube selection charts for R-22 is proposed. The set of charts takes into account of the roughness effect on the mass flow rate. For this purpose, a set of numerical model is developed and a series of experiments is conducted to verify the numerical model. A numerical model is used to calculated the mass flow rate for several sets of tube diameter, length, inlet pressures and degree of subcooling. The outlet of the tube is controlled to be at critical condition. The experimental flow rate is compared with calculated values. The calculated values are consistently less than the experimental ones except for the flow rate range below 40kg/hr. The deviation is within 10---. Based on the nunmerical model and results of experiments, the set of capillary tube selection charts for R-22 is constructed. The set of charts consists of standard capillary tube chart(L=2030mm, d=1.63mm, ${\varepsilon}=2.5{\mu}m$), non -standard flow factor(${\phi}_1$) chart, and non-standard roughness factor(${\phi}_2$) chart. The mass flow rate, flow factor, and the roughness factor are defined respectively as; $\dot{m}={\phi}_1{\phi}_2\dot{m}_{standard}\\{\phi}_1=\frac{\dot{m}(L,\;d,\;\varepsilon_{standard})}{\dot{m}_{standard}(L_{standard},\;d_{standard},\;{\varepsilon}_{standard})}\\{\phi}_2=\frac{\dot{m}(L_{standard},\;d_{standard},\;{\varepsilon})}{\dot{m}_{standard}(L_{standard},\;d_{standard},\;{\varepsilon}_{standard})}$.

• 한국산학기술학회논문지
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• 제22권3호
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• pp.316-322
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• 2021

본 연구의 목적은 물리치료사의 일과 삶의 균형이 직무열의에 미치는 영향을 분석하는데 있다. 연구대상은 U시 물리치료사 109명을 대상으로 실시하였다. 분석방법은 SPSS 22.0 프로그램을 사용하여 빈도분석, 신뢰도분석, 상관관계분석을 실시하였으며, 변수 간의 인과관계에 대한 다중회귀분석을 실시하였다. 연구결과 물리치료사의 일과 삶의 균형-갈등이 직무열의에 미치는 영향을 살펴본 결과 일과 삶의 균형의 하위요인인 일·가정 갈등, 가정·일 갈등은 직무열의에 유의미한 영향을 미치지 않는 것으로 나타났다. 물리치료사의 일과 삶의 균형-촉진이 직무열의에 미치는 영향을 살펴 본 결과 일과 삶의 균형의 일·가정 촉진, 가정·일 촉진 요인 모두 직무열의에 긍정적으로 유의미한 영향을 미치는 것으로 나타났다. 이는 물리치료사의 일과 삶의 균형-갈등 요인은 직무열의에 영향력을 미치지 못하지만 일과 삶의 균형-촉진의 일·가정 촉진, 가정·일 촉진 요인이 높을수록 직무열의가 높아진다는 것을 의미하는 것이다. 본 연구의 시사점은 물리치료사들의 일과 삶의 균형 유지 및 직무열의 증진을 위한 방안 마련의 기초자료가 될 것으로 사료된다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.162-162
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• 2010

InAs 는 광전자 및 광통신 소자에 널리 이용되는 $In_xGa_{1-x}As_yP_{1-y}$ 화합물의 endpoint 로서, Heterojunction Field-Effect Transistors (HEMTs), Heterojunction Bipolar Transistor (HBT) 등에 중요하게 이용되고, 다양한 소자의 기판으로도 폭넓게 사용되는 물질이다. InAs 의 반도체 소자로의 응용을 위해서는 정확한 광 특성과 밴드갭 값들이 필수적이며, 분광타원편광분석법(ellipsometry) 을 이용한 상온 InAs 유전율 함수는 이미 정확히 알려져 있다. 그러나 상온에서는 $E_2$ 전이점 영역에서 여러 개의 밴드갭들이 중첩되어 있어, 밴드구조계산 등에 필수적인 InAs의 전이점을 정확히 정의하기 어렵다. 또한, 현재의 산업계에서 중요하게 여겨지는 실시간 모니터링을 위해서는 증착온도에서의 유전율 함수 데이터베이스가 필수적이다. 이와 같은 필요성에 의해, 22 K - 700 K 의 온도범위에서 InAs 의 유전율 함수와 밴드갭 에너지에 대한 연구를 수행하였다. InAs bulk 기판을 methanol, acetone, DI water 등으로 세척 한 뒤, 저온 cryostat 에 부착하였다. 분광타원분석법은 표면의 오염에 매우 민감하기 때문에, 저온에서의 응결 방지를 위해 고 진공도를 유지하며, 액체 헬륨으로 냉각하였다. 0.7 - 6.5 eV 에너지 영역에서 측정이 가능한 분광타원편광분석기로 측정한 결과, 온도가 증가함에 따라 열팽창과 phonon-electron 상호작용효과의 증가에 의해, 밴드갭 에너지 값의 적색 천이와 밴드갭들의 중첩을 관찰 할 수 있었다. 정확한 밴드갭 에너지 값의 분석을 위하여 2계 미분을 통한 표준 밴드갭 해석법을 적용하였으며, 22 K 의 저온에서는 $E_2$ 전이점 영역에서 중첩된 여러 개의 밴드갭들을 분리 할 수 있었다. 또한 고온에서의 연구를 통해, 실시간 분석을 위한 InAs 유전함수의 데이터베이스를 확립하였다. 본 연구의 결과는 InAs 를 기반으로 한 광전자 소자의 개발 및 적용분야와 밴드갭 엔지니어링 분야에 많은 도움이 될 것으로 예상한다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권11호
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• pp.4499-4505
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• 2014

Glioblastoma, the most aggressive and malignant form of glioma, appears to be resistant to various chemotherapeutic agents. Hence, approaches have been intensively investigated to targeti specific molecular pathways involved in glioblastoma development and progression. Aloe emodin is believed to modulate the expression of several genes in cancer cells. We aimed to understand the molecular mechanisms underlying the therapeutic effect of Aloe emodin on gene expression profiles in the human U87 glioblastoma cell line utilizing microarray technology. The gene expression analysis revealed that a total of 8,226 gene alterations out of 28,869 genes were detected after treatment with $58.6{\mu}g/ml$ for 24 hours. Out of this total, 34 genes demonstrated statistically significant change (p<0.05) ranging from 1.07 to 1.87 fold. The results revealed that 22 genes were up-regulated and 12 genes were down-regulated in response to Aloe emodin treatment. These genes were then grouped into several clusters based on their biological functions, revealing induction of expression of genes involved in apoptosis (programmed cell death) and tissue remodelling in U87 cells (p<0.01). Several genes with significant changes of the expression level e.g. SHARPIN, BCAP31, FIS1, RAC1 and TGM2 from the apoptotic cluster were confirmed by quantitative real-time PCR (qRT-PCR). These results could serve as guidance for further studies in order to discover molecular targets for the cancer therapy based on Aloe emodin treatment.

• 한국강구조학회 논문집
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• 제22권1호
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• pp.43-53
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• 2010

면진기술은 건축물의 구조적 피해를 줄이고 진동에 민감한 구조물의 내진성능을 향상시키는 가장 효과적인 방법 중의 하나이다. 하지만 면진구조시스템에서 지진에너지를 흡수하는 댐퍼의 재료로서 자주 사용되었던 납의 환경오염문제가 대두되면서, 최근에는 친환경 재료인 강재를 사용하는 경우가 증가하고 있다. 본 연구에서는 면진시스템용 에너지흡수장치인 U형 강재댐퍼를 대상으로 비선형 유한요소해석을 수행하고 SS400 강재와 고인성강재를 사용한 댐퍼의 해석결과를 비교하여 사용강재의 재료적 특성의 차이가 댐퍼의 변형능력에 미치는 영향을 분석하며, 고인성강재를 사용한 댐퍼의 형상비, 두께, 폭에 따른 변형능력을 파악한다. 또한 고인성강재를 사용한 U형 강재댐퍼의 응력분포를 분석하여 응력분포에서 나타내는 문제점을 개선하기 위한 대책으로 댐퍼의 일부 구간에 개구부를 둔 새로운 형상을 제시하고 형상에 따른 변형능력, 내력특성 및 응력분포를 고려하여 최적형상과 설계식을 제안하고자 한다.

• 한국가축번식학회지
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• 제20권2호
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• pp.163-170
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• 1996

The effect of several potential antioxidants and amino acids were examined as a means of increasing the development of in vitro matured and in vitro fertilized oocytes into morulae or blastocysts. Bovine embryos developed to the 2~8 cell stage after in vitro fertilization were cultured for 5 to 6 days at 39$^{\circ}C$ in CR1aa containing varing concentraton of the antioxidants and amino acid in a gas phase consisting of 5% CO2, high humidified air. At 5~6 days, embryo developments were reduced, and embryos were fixed and stained with Hochest 33342 DNA stain to facilitate counting of cells. In experiment 1, the proportion of embryos developed to morulae and blastocysts in CR1aa containing 1mM, 2.5mM taurine (22.6% and 20.4%) was slightly higher than those of 0, 5 and 10mM Taurine (5.7, 5.7 and 3.9%, P<0.05). In experiment 2, addition of glutathione did not improve blastocyst development (P>0.05). In experiment 3, concentations of superoxide dismutase(SOD) ranging from 300 to 1,000 U did not affect the propotion of embryos developing into blastocysts (P>0.05). In experiment 4, addition of 250 U catalase(38.5%) was slighty higher than those of 0, 500 and 1,000U. In experiment 5, the proportion of embryo developed beyond morula stage in CR1aa with taurine plus EDTA was slighty higher than other treatments(15.7, 26.0 and 29.2%), there were no significantly increases in cell number among treatments(P>0.05). These results are indicating that antioxidants and amino acids can increase the proportion of embryos that develop into morulae and blastocysts, but did not increas in cell number of blastocysts.

• Journal of Microbiology and Biotechnology
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• 제22권1호
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• pp.58-68
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• 2012

An investigation was conducted on the enhancement of production and purification of an oxidant and SDS-stable alkaline protease (BHAP) secreted by an alkalophilic Bacillus horikoshii, which was screened from the body fluid of a unique Korean polychaeta (Periserrula leucophryna) living in the tidal mud flats of Kwangwha Island in the Korean West Sea. A prominent effect on BHAP production was obtained by adding 2% maltose, 1% sodium citrate, 0.8% NaCl, and 0.6% sodium carbonate to the culturing medium. The optimal medium for BHAP production contained (g/l) SBM, 15; casein, 10; $K_2HPO_4$, 2; $KH_2PO_4$, 2; maltose, 20; sodium citrate, 10; $MgSO_4$, 0.06; NaCl, 8; and $Na_2CO_3$, 6. A protease yield of approximately 56,000 U/ml was achieved using the optimized medium, which is an increase of approximately 5.5-fold compared with the previous optimization (10,050 U/ml). The BHAP was homogenously purified 34-fold with an overall recovery of 34% and a specific activity of 223,090 U/mg protein using adsorption with Diaion HPA75, hydrophobic interaction chromatography (HIC) on Phenyl-Sepharose, and ion-exchange chromatography on a DEAE- and CM-Sepharose column. The purified BHAP was determined a homogeneous by SDS-PAGE, with an apparent molecular mass of 28 kDa, and it showed extreme stability towards organic solvents, SDS, and oxidizing agents. The $K_m$ and $k_{cat}$ values were 78.7 ${\mu}M$ and $217.4s^{-1}$ for N-succinyl-Ala-Ala-Pro-Phe-pNA at $37^{\circ}C$ and pH 9, respectively. The inhibition profile exhibited by PMSF suggested that the protease from B. horikoshii belongs to the family of serine proteases. The BHAP, which showed high stability against SDS and $H_2O_2$, has significance for industrial application, such as additives in detergent and feed industries.

• 자원환경지질
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• 제18권3호
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• pp.239-246
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• 1985

Uranium-thorium separation technique from shallow sediment and ${\alpha}$-source electrodeposition technique are established in the present work, and uranium series disequilibrium dating method is applied to the quaternary piston core from Sangback-do, South Sea of Korean Peninsula. The age of the piston core (depth 200~300cm) sampled from Latitude $34^{\circ}06^{\prime}37^{{\prime}{\prime}}$, Longitude $127^{\circ}37^{\prime}37^{{\prime}{\prime}}$ was determined as ranging from $9.0{\pm}2.2$ to $22.9{\pm}3.3$ Ky B.P, i.e., Later Pleistocene, older than Holocene which was stratigraphically known in the previous study. The sedimentation rate in the area is regularly increased according to the depth of the sedimentation layer. Except a few split sections of the piston core the sedimentation rates are avarage 7.2cm/1,000 years. Where sedimentation layer is deeper, then sedimentation rate tend to be slower. It must be pointed in age calculation that initial $^{230}Th/^{234}U$ radioactivity ratio is much influenced when detrital material was introduced in the sample. The $^{230}Th/^{234}U$ activity ratio measured in the present work is 0.06 at $^{232}Th=0$, and this value shows higher analytical errors because of the low· radioactivity of $^{230}Th$ in the samples.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제10권S_3호
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• pp.113-119
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• 2001

Surface sediments(0~5 cm) were sampled from 22 stations in Ulsan Bay, one of the most highly industrialized regions in Korea, in November 2000. The sediment samples were analyzed for their polycyclic aromatic hydrocarbon(PAH) content using a gas chromatography coupled to a mass spectrometer detector(GC/MSD). The total PAH concentrations in the sediments varied from 14 to 7108 ng/g dry weight with a mean value of 1052 ng/g dry weight. The level of carcinogenic PAHs ranged from 6 to 2396 ng/g dry weight with a mean value of 433 ng/g dry weight. The highest PAH concentrations in the sediments from Ulsan Bay were found at Station U8, whereas the lowest levels were observed at Stations U2 and U 17. The PAH distribution exhibited a decreasing gradient from the inner basin to the outer bay. The predominant contributors to the aromatic ring groups of the 16 PAHs were four- and five-ring groups, such as fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzor[b]fluoranthene, benzor[k]fluoranthene and benzo[a]pyrene, while two- and three-ring aromatics, like naphthalene, acenapthylene, acenaphthene, fluorene, phenanthrene and anthracene, only exhibited a low concentration. The molecular indices for phenanthrene/anthracene and fluoranthen/pyrene were used to Identify the origin of the PAH contamination in the sediments. The results indicated that the PAH contamination in Ulsan Bay was mostly Pyrolytic in origin with a Petrogenic input adjacent to Ulsan and Jangsuengpo harbor.

• KSBB Journal
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• 제22권2호
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• pp.114-118
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• 2007

제주도 토양 샘플에서 분리한 amylase 생산균 15번 균주는 100 mL flask level와 10 L 생물반응기에서 T. inhamatum KSJ1의 FPpase, amylase 효소활성과 거의 비슷하였으며, flask level에서 두 균주의 xylanase, avicelase, CMCase, Gluco-amylase, $\beta$-amylase의 효소활성 및 음식물쓰레기의 당화능력도 모두 거의 비슷하였다. 두 균주를 YMEA 고체배지와 광학현미경으로 그 형태학적 특징을 관찰한 결과 T. inhamatum KSJ1이 녹색빛을 띠는 것과는 달리 amylase 생산균 15번 균주는 황색빛을 띠는 사상균으로 확인되었으며, 두 균주는 동일한 균은 아니지만 동일한 속으로 사료된다.