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Effects of Extruded Corn in Nursery and Finishing Pigs (자돈 및 비육돈에 있어 옥수수의 가공 효과)

  • Han, Y.K.;Kim, I.H.;Hong, J.W.;Kwon, O.S.;Min, B.J.;Lee, W.B.;Shon, K.S.;Lee, J.H.
    • Journal of Animal Science and Technology
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    • v.45 no.6
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    • pp.949-956
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    • 2003
  • For the Exp. 1, a total of sixty pigs(15.95${\pm}$0.09kg average initial body weight) were used in a 28-d growth assay to determine the effects of extruded chinese corn on growth performance and nutrient digestibility in nursery pigs. Dietary treatments included 1) UCORN(U.S. corn-SBM based diet), 2) CCORN(Chinese corn-SBM based diet) and 3) ECCORN(Extruded Chinese corn-SBM based diet). Overall period, average daily gain of pigs fed ECCORN diet was higher than that of pigs fed CCORN diet(547 vs 522 g/d), however, there was not significant difference. On day 10 of the experiment, pigs fed UCORN and ECCORN diet had significantly increased in DM and DE digestibilities compared to pigs fed CCORN diet(P〈0.05). Also, on day 24 of the experiment, pigs fed UCORN and ECCORN diet had a significant increase in DM digestibility compared to pigs fed CCORN diet(P〈0.05). Pigs fed ECCORN diet had significantly increased DE digestibility compared to pigs fed CCORN diet(P〈0.05). For the Exp. 2, three cannulated barrows(54.09kg average initial body weight) were used to determine the apparent ileal digestibilities of amino acids and nutrient digestibility of extruded corn in finishing pigs. Dietary treatments were the same as in Exp. 1. Apparent ileal digestibility of cystine was greater for UCORN and ECCORN than for CCORN(P〈0.05). Apparent digestibility of DM at the total tract was greater for UCORN and ECCORN than for CCORN(P〈0.05). Pigs fed UCORN and ECCORN diet had a significant increase in apparent total tract digestibility of N compared to pigs fed CCORN diet(P〈0.05). In conclusion, the results obtained from these feeding trials suggest that the extruded corn for nursery pigs had affected growth performance and DM and DE digestibilities. In finishing pigs, extruded corn was an effective means to improve apparent total tract digestibilities of DM and N.

G-, C-, and NOR-banding of Korean Native Pig Chromosomes (한국재래돼지의 G-, C-, 및 NOR-banding)

  • Sohn, S.H.;Kweon, O.S.;Baik, K.H.;Jung, W.;Cho, E.J.;Kang, M.Y.
    • Journal of Animal Science and Technology
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    • v.45 no.6
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    • pp.901-910
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    • 2003
  • Using the G-, C-, and NOR-banding techniques, a karyotyping for Korean Native Pig was performed. Blood samples were collected from 50 male Korean Native Pigs that had been bred at the National Livestock Research Institute and then blood cells were prepared from in vitro cultures followed by karyotyping; G-, C-, and NOR-banding patterns of metaphase chromosomes were analyzed. The karyotype of Korean Native Pig is 38, XX or XY which consists of 5 pairs of submetacentric chromosomes(Group I), 2 pairs of acrocentric chromosomes with short p-arm(Group II), 5 pairs of medium metacentric chromosomes(Group III), 6 pairs of acrocentric chromosomes(Group IV) and metacentric X and Y sex chromosomes. On GTG-banding, the Korean Native Pig exhibited a typical and identical banding pattern in each homologous chromosomes. Overall chromosomal morphology and positions of typical landmarks of the Korean Native Pig were virtually identical to those of Committee for the Standardized Karyotype of the Domestic Pig(CSKDP). However, numbers of G-bands of the Korean Native Pig chromosomes were more than those of CSKDP. In chromosomes 1, 3, 5, 6, 7, 8, 13, 14, 15, 16, 17, 18 and X, the Korean Native Pig exhibited more separated bands as compared with CSKDP. In C-banding patterns, although the quantity of heterochromatin was variable in each chromosome, most of the Korean Native Pig chromosomes had heterochromatic C-bands on centromeres. However, the heterochromatic C-band was constantly observed on the whole Y chromosome. In AgNOR staining, the NORs were located at centromeres on the chromosomes 8 and 10. The number of NORs per metaphase ranged from 2 to 4 giving a mean value of 2.13. The number of NORs were distributed on all chromosome pair 10 but not on chromosome 8. The sizes of NORs were also differed between homologous chromosomes 8. Numbers of NORs of Korean Native Pig were significantly higher than those of Yorkshire. The pattern of pig NORs was polymorphic in breeds, individuals and cells, especially on chromosome 8.

Association Between the Polymorphism in the Promoter Region of Porcine A-FABP Gene and Growth Traits in Duroc Pigs (돼지 Duroc 품종에서 A-FABP promoter의 다형성과 성장형질의 연관성)

  • Han, Sang-Hyeon;Jo, In-Cheol;Lee, Jong-Eon;Kim, Hyo-Seon;Lee, Jeong-Gyu;Jeon, Jin-Tae;O, Mun-Yu;Go, Mun-Seok
    • Journal of Animal Science and Technology
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    • v.48 no.1
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    • pp.21-28
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    • 2006
  • A polymorphism was found in the promoter region of porcine adipocyte fatty acid binding protein gene(A-FABP) gene which plays a key role in the binding and transportation of free fatty acid in adipocyte and deposition of intramuscular fat. Mutation was detected a substitution(T406C) using SSCP analysis and subsequently confirmed by sequencing the fragment in Duroc pigs. This T-406C mutation might change the binding activity for transcription factor nuclear factor 1(NF1). In this population, this mutation was genotyped using HinfⅠRFLP, and found three kinds of genotypes(TT, TC, and CC) showing their frequencies of 42.3, 44.3, and 13.4%, respectively. We statistically analyzed the association between the A-FABP genotypes and growth traits and found that the body weights of the pigs containing 406C/(TC or CC) were heavier for the body weight at the age of 20 weeks than those containing genotype TT(P<0.05), but not for those at the age of 0, 3, and 10 weeks. Pigs containing genotype CC had also a higher value for the average daily gain and lower values for the date for 90kg of body weight and food conversion ratio than those of 406T/- genotype. In addition, without the significant difference of back fat thickness, there was a significant association between the existence of allele CC and lean meat and eye muscle area(P<0.05). As a result of this study, we suggest that the allele T406C in the promoter region of A-FABP gene play an important role in deposition of intramuscular fat and weight in the later growth period. This polymorphism will be an useful molecular marker for breeding of Duroc pigs.

On the Microdistribution of Fresh-Water Fish, Coreoleuciscus splendidus(Gobioninate) from Korea (한국산(韓國産) 담수어(淡水魚), 쉬리 Coreoleuciscus splendidus의 미세분포(微細分布)에 관(關)하여)

  • Choi, Ki-Chul;Choi, Shin-Sok;Hong, Young-Pyo
    • Korean Journal of Ichthyology
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    • v.2 no.1
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    • pp.63-76
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    • 1990
  • Studies on the microdistribution, distributional aspect and affinity of Korean endemic species, SHIRl, Coreoleueiseus splendidus in th southern half of the Korean peninsula were carried out from May, 1966 to November, 1989. This species was found in 14 independent rivers and streamlets of the south Korea ; Yimjin R., Han R., Kum R., Ungchun S., Mangyung S., Dongjin R., Somjin R., Kwangyang East S., Sachun S., Gonyang S., Nakdong R., Samchukosip S., Samhwa S. of Namhae Island and Guchun S. of Geojae Islands, and were not found from Youngsan R., Ansung S. and Sabgyo S. This species was not common and independent comprising less than 25% of the total fish population in all habiting sites. Distributional zone was in the middle or upper streaches of main streams and tributaries. The average slope of distributional zone was 2.6, minimum slope was 0.29 and maximum slope was 5.56. Thirty-three species were found to occur along with this fish and 10 species of them occurred with more than 50% frequency. They were; 20 sp. of Cyprinidae, 5 sp. of Cobitidae, 1 sp. of Salmonidae, 1 sp. of Bagfridae, 1 sp. of Siluridae, 2 sp. of Amblycipitidae, of Serranidae, and 1 sp. of Eleotridae. Some species of fish appeared to have the same ecological position even though they have different distributional zones. These species were as follows; Acheilognathus signifer and A. limbata, Micriophsogobio koreensis and M. yaluensis in Cyprinidae, Cobitis koreensis, C. longicorpus, C. rotundicaudata in Cobitidae, Liobagrus andersoni and L. mediadiposalis in Amblycipitidae.

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Quality Characteristics and Retarding Retrogradation of Sponge Cakes containing Red Yeast Rice(Monascus nuruk) Flour (홍국(Monascus nuruk) 분말을 첨가한 스폰지 케이크의 품질 특성 및 노화 억제 분석)

  • Song, Ka-Young;Kim, Jong-Hee;O, Hyeon Bin;Zhang, Yangyang;Kim, Young-Soon
    • Culinary science and hospitality research
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    • v.22 no.3
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    • pp.11-21
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    • 2016
  • This study investigated the quality characteristics and retarding retrogradation of sponge cakes made with red yeast rice (RYR) flour. RYR (Monascus nuruk) is known to help digestion, smooth blood flow, and have anti-cancer, anti-microbial, and inhibitory effects against biosynthesis of cholesterol and blood pressure. This studys aim' was to find the optimal proportion of RYR flour in sponge cake. RYR sponge cakes were prepared with various levels (0, 5, 10, 15 and 20%) replacement of wheat flour and were designated as the control (without RYR), RYR5, RYR10, RYR15 and RYR20 respectively. Specific gravity was the lowest in RYR15 at 0.57, and the baking loss rate was not significantly different among the samples (p<0.05). The dough yield was the highest in RYR15 at 96.61. The moisture contents was highest in order, control, RYR5, and RYR15 at 28.67%, 28.18%, and 26.82% respectively. The L-value of crust tended to increase according to the level of RYR, but the L-value of crumb decreased in accorddance with the the content of RYR. The a-value of crust also decreased according to the level of RYR, although the a-value of crumb increased in response to higher levels of RYR. The b-value tended to decrease with increases of RYR (p<0.05). RYR5 exhibited the highest pH at 8.63, compared with RYR15 (8.57). The hardness, which was measured after cooling for 1 hour, was the lowest in RYR15 at $163.33g/cm^2$ and the springiness was not different significantly (p<0.05). Cohesiveness was the highest in RYR10 at 133.06%. The chewiness was the highest in RYR10 at $391.63g{\cdot}cm$ and lowest in RYR15 ast $169.62g{\cdot}cm$. Avrami equation showed that RYR15 and RYR20 had the lowest Avrami exponent (n) at 0.0664 and 0.4983 respectively. Time constant (1/k) was the highest in RYR15 at 200.00. Sensory evaluation revealed that RYR15 was the highest in color (5.50), flavor (4.95), sweetness (4.90), chewiness (4.75), and overall acceptability (4.60).

The Geochemistry of Copper-bearing Hydrothermal Vein Deposits in Goseong Mining District (Samsan Area), Gyeongsang Basin, Korea (경상분지내 삼산지역 열수동광상에 관한 지화학적 연구)

  • Choi, Sang Hoon;So, Chil Sup;Kweon, Soon Hag;Choi, Kwang Jun
    • Economic and Environmental Geology
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    • v.27 no.2
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    • pp.147-160
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    • 1994
  • Copper-bearing hydrothermal vein mineralization of the Samsan area was deposited in two stages (I and II) of quartz-calcite-sulfide veins which fill fissures in Cretaceous volcanic and sedimentary rocks of the Gyeongsang basin. The major ore minerals, chalcopyrite and sphalerite, together with pyrite, galena, hematite, and minor sulfosalts, occur with epidote and chlorite as gangue minerals in stage I quartz veins. Chlorite geothermometry, fluid inclusion and stable isotope data indicate that copper ore was deposited mainly at temperatures between $330^{\circ}C$ and $280^{\circ}C$ from fluids with salinities between 12 and 3 equiv. wt % NaCl. Evidence of fluid boiling indicates a range of pressures from ${\leq}100$ to 200 bars bars. Within ore stage I there was an apparent decrease in ${\delta}^{34}S$ values of $H_{2}S$ with paragenetic time, from 8.0 to 2.3 per mil. This pattern was likely achieved through progressive increases in activity of oxygen accompanying boiling and mixing. In the early part of the first stage, the high temperature, high salinity fluids gave way to progressively cooler and more dilute fluids of the late parts in the first stage and of the second stage. There is a systematic decrease in calculated ${\delta}^{18}O_{water}$ values with decreasing temperature in the Samsan hydrothermal system, from values of -86 per mil for early portion of stage I through -5.9 per mil for late portion of stage I to -6.3 per mil for stage II. The ${\delta}D$ values of fluid inclusion waters also decrease with paragenetic time from -76 per mil to -86 per mil. These trends combined with mineral paragenesis and fluid inclusion data are interpreted to indicate progressive cooler, more oxidizing meteoric water inundation of an early exchanged meteoric hydrothermal system.

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Analysis of Hydrocarbon Trap in the Southwestern Margin of the Ulleung Basin, East Sea (동해 울릉분지 남서주변부의 탄화수소 트랩 분석)

  • Lee, Minwoo;Kang, Moo-Hee;Yoon, Youngho;Yi, Bo-Yeon;Kim, Kyong-O;Kim, Jinho;Park, Myong-ho;Lee, Keumsuk
    • Economic and Environmental Geology
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    • v.48 no.4
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    • pp.301-312
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    • 2015
  • A commercial gas field was found in the southwestern continental shelf of the Ulleung Basin, East Sea in the late 1990s. To develop additional gas field, an exploration well was drilled through the coarse infill of submarine canyon near the gas field, but it was uneconomic to develop hydrocarbons. Using newly acquired deep seismic reflection and previous well data, we have identified additional geological structure which has hydrocarbon potentials below submarine canyons in the southwestern margin of the basin. Based on the interpretation of the deep seismic reflection and well data, the sequences of the study area can be classified into the syn-rift megasequence(MS1), post-rift megasequence(MS2), syn-compressional megasequence(MS3), and post-compressional megasequence(MS4) in relation to the tectonic events. MS1, deposited simultaneously with the basin formation before the middle Miocene, is characterized by chaotic seismic facies with low- to moderate-amplitude and low frequency reflections. MS2 comprises laterally continuous, low- to moderate-amplitude reflections, showing progradational stacking patterns due to high rates of sediment supply during basin expansion in the middle Miocene. MS3 is mainly composed of continuous reflections with high amplitude and moderate- to high-frequency which are interpreted as coarse-grained sediments. The coarse-grained sediments of MS3 sequence is widely truncated by several submarine canyons which filled with fine-grained sediment of MS4 to form a stratigraphic trap of hydrocarbon. Therefore, the reservoir and seal of the hydrocarbon trap in the study area are coarse-grained sediment of MS3 and submarine canyon filled with fine-grained sediment of MS4, respectively. A flat-spot seismic anomaly, which may indicate the presence of hydrocarbon, is observed within the stratigraphic trap.

Antitumor and Free Radical-Scavenging Activities of Various Extract Fractions of Fruits and Leaves from Prunus mume (금매와 매화 잎 추출물의 프리라디칼 억제 활성 및 항암 효과)

  • Rho, Kyu-A;Kim, Gyeong-Ji;Ji, Hyun-A;Lim, Han-Sol;Chung, Kang-Hyun;Lee, Kwon-Jai;Song, Byeong Chun;An, Jeung Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.8
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    • pp.1137-1143
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    • 2015
  • This study investigated the free radical-scavenging and antitumor activities of hot water, water, acetone, ethanol, ethyl acetate, chloroform, and hexane extracts of fruits and leaves from Prunus mume. The various extracts were evaluated for their total polyphenol, flavonoid, and tannin contents, scavenging activities by DPPH and ABTS analyses, reducing power, protective effects against oxidative stress in L-132 cells, and antitumor activities against A549, HeLa, and U87 cancer cells. Ethanol extracts of fruits and leaves showed the highest total polyphenol content (336.41 and 523 mg GAE/100 g, respectively). DPPH and ABTS radical-scavenging activities increased according to concentration of fruit. DPPH radical-scavenging activity of ethanol extracts from leaves was 65.48% at $200{\mu}g/mL$. All extract fractions of leaves showed high ABTS radical-scavenging activities. The reducing power activities increased according to increasing concentration of fruits and leaves. All extracts of leaves performed better than extracts of fruits in terms of protective effects against oxidative stress in L-132 cells. Ethyl acetate, chloroform, hexane, ethanol extracts of fruits and leaves showed anticancer activities against A549, HeLa, and U87 cancer cells. However, ethanol extracts of fruits and leaves showed no toxicity in normal cells (BNLCL2). This study suggests that antioxidant activities of fruits and leaves from P. mume depend on polyphenol contents. Thus, fruits and leaves from P. mume can be useful as natural antioxidant compounds.

A study about the relation between elev ated transaminase lev el and severity of viral lower respiratory tract infection in children (바이러스성 하기도 감염 환자에서 간기능 이상과 중증도와의 연관성에 관한 연구)

  • Lee, Chul Hee;Shin, Seon Hee;Lee, Jung Won;Sung, Tae Jung;Kim, Sung Goo;Lee, Kyu Man
    • Pediatric Infection and Vaccine
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    • v.14 no.1
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    • pp.47-54
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    • 2007
  • Purpose : Viral respiratory tract infection is most common cause for admission to hospital in children. There are many cases with elevated transaminase level in patients with viral lower respiratory tract infection (LRTI). The aim of this study was to compare indexes of disease severity such as duration of assisted ventilation, length of hospital stay and Respiratory Distress Assessment Instrument (RDAI) score in children with viral LRTI with and without elevated transaminase levels and to determine the etiology related to elevated transaminase levels in this patients group. Methods : Virological analysis was done from respiratory specimens obtained from patients with LRTI admitted to Kangnam Sacred Heart Hospital from Jan. 2003 to Jun. 2005. Viral diagnosis was made by isolation of viruses employing HEp-2 cell culture from nasopharyngeal aspiration. Medical records of children were reviewed retrospectively. We compared age, sex, RDAI score, Respiratory Rate (RR) score and mean duration of hospital stay between patients with elevated transaminase levels (Patient Group) and patients with normal transaminase levels (Control Group). Results : Viruses were isolated from 181 children with LRTI. 16 cases were excluded according to criteria. 28 cases (17.0%) had elevated transaminase levels (Patient group) and 137 cases (83.0%) had normal transaminase levels (Control group). There were no significant difference in duration of fever, RR score, RDAI score, incidence of $O_2$ inhalation and duration of hospital stay between patient group and control group. We found 17 (60.7%) cases of RSV, 4 cases (14.3%) of parainfluenza, 4 cases (14.3%) of influenza B virus, 3 cases (10.7%) of adenovirus and 1 case (3.6%) of influenza A virus infection in patient group and 78 cases (56.9%) of RSV, 28 cases (20.4%) of parainfluenza virus, 13 cases (9.5%) of influenza A virus, 9 cases (6.6%) of influenza B virus, 6 cases (4.4%) of adenovirus and 3 cases (2.2%) of coxsackie virus infection in control group. Conclusion : There were 28 cases (17.0%) with elevated transaminase level among patients with virus isolated LRTI. There was no relation between elevated transaminase level and severity of disease. The viral etiologies in two groups were not significantly different. There was no significant difference of age distribution between two groups.

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Relationship Between Nutrient Intakes and Blood Biochemical Parameters of Korean Female Subjects Classified by Eight Constitution Medicine (8체질로 분류한 한국 여성의 영양소 섭취 수준과 혈중 지질 농도와의 관계)

  • Cho, B.J.;Kim, M.J.;Kim, M.J.;Song, Y.O.
    • The Journal of Korean Medicine
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    • v.34 no.3
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    • pp.143-152
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    • 2013
  • Objectives: To investigate the relationship between nutrient intakes and blood biochemical parameters of Korean women classified by the Eight Constitutions. Methods: The constitutions of female subjects were determined by the methods of eight constitutional pulse formation. Anthropometric characteristics and blood biochemical parameters including fasting blood glucose (FSB), serum lipid profiles, homocysteine, and insulin were determined. Food frequency questionnaires were used to evaluate the nutrient intake of the subjects. Results: Subjects were classified as Hepatonia 16.8%, Cholecystonia 2.8%, Pancreotonia 58.9%, Pulmotonia 5.1%, Colonotonia 16.4%, respectively. Gastrotonia, Renotonia, and Vesicotonia individuals were not present in this study. BMI, total calorie and fat intakes of the Mok constitutions (Hepatonia and Cholecystonia) were higher than those of the Gum constitutions (Pulmotonia and Colonotonia) (p<0.05). Triglyceride, total cholesterol, and LDL-C concentrations for the Cholecystonia were the highest while those for the Pulmotonia (p<0.05) were lowest, which is in line with the results from nutrient intakes. Conclusions: Total calorie and fat intake among subjects with different constitutions were different. BMI and serum lipid profiles positively associated with calorie and nutrients intakes were significantly higher in Mok constitutions than Gum constitutions. Personalized diet plans seem to be needed for subjects on a special diet due to the individual constitutional differences.