Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Association Between the Polymorphism in the Promoter Region of Porcine A-FABP Gene and Growth Traits in Duroc Pigs

돼지 Duroc 품종에서 A-FABP promoter의 다형성과 성장형질의 연관성

  • 한상현 (농촌진흥청 난지농업연구소) ;
  • 조인철 (농촌진흥청 난지농업연구소) ;
  • 이종언 (농촌진흥청 난지농업연구소) ;
  • 김효선 (경상대학교 응용생명과학부) ;
  • 이정규 (경상대학교 응용생명과학부) ;
  • 전진태 (경상대학교 응용생명과학부) ;
  • 오문유 (제주대학교 생명과학과) ;
  • 고문석 (농촌진흥청 난지농업연구소)
  • Published : 2006.02.28
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Abstract

A polymorphism was found in the promoter region of porcine adipocyte fatty acid binding protein gene(A-FABP) gene which plays a key role in the binding and transportation of free fatty acid in adipocyte and deposition of intramuscular fat. Mutation was detected a substitution(T406C) using SSCP analysis and subsequently confirmed by sequencing the fragment in Duroc pigs. This T-406C mutation might change the binding activity for transcription factor nuclear factor 1(NF1). In this population, this mutation was genotyped using HinfⅠRFLP, and found three kinds of genotypes(TT, TC, and CC) showing their frequencies of 42.3, 44.3, and 13.4%, respectively. We statistically analyzed the association between the A-FABP genotypes and growth traits and found that the body weights of the pigs containing 406C/(TC or CC) were heavier for the body weight at the age of 20 weeks than those containing genotype TT(P<0.05), but not for those at the age of 0, 3, and 10 weeks. Pigs containing genotype CC had also a higher value for the average daily gain and lower values for the date for 90kg of body weight and food conversion ratio than those of 406T/- genotype. In addition, without the significant difference of back fat thickness, there was a significant association between the existence of allele CC and lean meat and eye muscle area(P<0.05). As a result of this study, we suggest that the allele T406C in the promoter region of A-FABP gene play an important role in deposition of intramuscular fat and weight in the later growth period. This polymorphism will be an useful molecular marker for breeding of Duroc pigs.

지방세포 내에서 자유 지방산과의 결합수송과 근내지방의 축적에 핵심적인 역할을 담당하는 지방세포 지방산결합단백질 유전자(A-FABP)의 promoter 영역에서 다형성(T-406C)를 SSCP에 이은 DNA 염기서열 분석을 통해 돼지 Duroc 품종에서 확인하였다. 이 돌연변이 406C는 전사인자 nuclear factor 1의 결합 활성을 변화시킬 수 있는 돌연변이로 확인되었다. Duroc 집단에서 HinfⅠ-RFLP 방법으로 유전자형을 결정한 결과 세 종류(TT, TC, CC)의 유전자형을 확인하였고, 빈도는 각각 42.3, 44.3, 13.4%로 확인되었다. 발견된 유전자형과 성장형질간의 상관관계를 통계적으로 유의성을 분석했을 때, 406C/ (TC와 CC)인 돼지의 20주령 체중이 TT인 돼지에서보다 더 무거운 양상을 보이며 유의한 차이를 보였고(P<0.05), 생시체중, 3주령, 10주령 체중에서는 유의한 차이가 발견되지 않았다. 동형접합인 406C/C 돼지들은 406T/인 돼지들에 비해 일당증체량, 90kg 도달일령, 사료효율 면에서 유의적인 차이를 나타내었다. 또한, 등지방두께의 변화와는 무관하게 등심단면적과 정육률에서 유의적인 차이를 확인하였고, CC 동형접합인 돼지에서 더 높은 수치를 나타내었다(P<0.05). 이상의 결과들을 토대로, A-FABP 유전자의 promoter 상에서 발견된 돌연변이인 T-406C는 생육 후반기에서 체중과 등심단면적의 증가에 중요한 역할을 담당하고 있다고 사료되며, 생육 후기 근내지방의 축적과 관련되어 있는 것으로 사료된다. 본 연구에서 발견한 유전자의 다형성은 Duroc 품종의 육종을 위한 분자 marker로서 유용하게 이용될 것으로 사료된다.

Keywords

References

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