• 대한의용생체공학회:의공학회지
• /
• 제8권1호
• /
• pp.87-92
• /
• 1987

In order to evaluate the artificial skin for burn would covering materials, copoly(N. carbobenzoxy-L-Iysine-L-leucine)s were prepared by Ipolymerization of N - carbobenzoxy-L- I sine anhydride and L-leucine anhydride in homogeneous solvents using triethylamine as an initiator. The synthetic polypeptides and the oxter type polyurethane(PV)of medical grade were used as the sheet type membranes were prepared ; monolayer membrances were composed of only the polypeptides, bilayer membranes and blend membranes were controlled by composition of the polypeptides and PU. Test of the swelling degree, mechanical tensile strength, elongation, oxygen permeability, water-vapor loss and In vitro degradation treated by pretense TV of samples of artificial skin were measured by adequate methods so as to mechanical, physincal characterization and biodegradation. As a result, all the values of samples were found to be similar to desired value of skin which was nature. The Artificial skin based on polypeptides can be considered as ideal burn wound covering materials.

• Archives of Plastic Surgery
• /
• 제35권2호
• /
• pp.127-133
• /
• 2008

Purpose: The purpose of this study is to evaluate the remodeling process of the various skin substitutes in 4th and 6th weeks following the transplantation when transplanted onto nude mice. Methods: Three types of artificial skin substitutes, such as PLGA scaffold with keratinocyte sheets(group 1), acellular human dermis($Surederm^{(TM)}$) and keratinocyte sheet(group 2), bioengineered skin($Neoderm^{(TM)}$)(group 3), were applied to the wound on nude mice. All mice were killed in 2, 4 weeks and/or 6 weeks after grafting and tissue samples were harvested from the back of mice. The changes in wound size, degree of angiogenesis, formation of basement membrane and epidermis, density of collagen fibers and neural restoration were examined. Results: There was no significant changes in wound size among the three groups. However, the size of wound decreased in the non-substituted group due to contracture. Degree of angiogenesis and systhesis of collagen or neurofilaments were mostly increased in bioengineered skin($Neoderm^{(TM)}$)(group 3), followed by acellular human dermis($Surederm^{(TM)}$) and keratinocyte sheet(group 2), PLGA scaffold with keratinocyte sheets (group 1). However, group 3 and group 2 showed similar thickness of basement membrane and epidermis. Conclusion: We found that degree of angiogenesis, formation of basement membrane and skin appendages, density of collagen fibers and neurofilaments can be the categories to evaluate the success of artificial skin substitution in early stages.

• 대한화장품학회:학술대회논문집
• /
• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
• /
• pp.659-659
• /
• 2003

In this study, we have investigated the potent anti-aging effect of DA-3711, a cosmetic ingredient derived from artificial skin culture. The artificial skin was originally developed as a skin replacement for the treatment of chronic skin wounds. To produce DA-3711, neonatal human fibroblasts were seeded into biocompatible collagen/chitosan/glycosaminoglycan (GAG) scaffolds and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with fetal bovine serum and nonessential amino acids. Analysis of the culture broth (DA-3711) showed that growth factors such as VEGF, TGF-$\beta$, KGF were present at significantly higher levels that in the culture broth of fibroblasts cultured in monolayer. The biological activity of DA-3711 was assessed by measuring in vitro cell proliferation and collagen synthesis of normal human fibroblasts. Fibroblasts treated with 10％ DA-3711 showed a 2-fold higher proliferation and 2 to 4-fold higher collagen synthesis than untreated cells. DA-3711 also exhibited anti-oxidative effects, since cells under peroxide-induced oxidative stress showed a 30％ higher viability in DA-3711-containing medium than in medium without DA-3711 addition. The results suggest that DA-3711 may have anti-aging effects by stimulating skin regeneration and protecting against oxidative stress.

• 한국컴퓨터정보학회논문지
• /
• 제27권8호
• /
• pp.241-251
• /
• 2022

화장품 및 뷰티산업에서 고객의 피부상태 진단과 관리는 중요한 필수기능이다. 소셜미디어 환경이 사회 전 분야에 확산되고 일반화되면서 피부 상태의 진단과 관리에 대한 다양하고 섬세한 고민과 요구 사항의 질문과 답변의 상호작용이 소셜미디어 커뮤니티에서 활발하게 다루어지고 있다. 그러나 소셜미디어 정보는 매우 다양하고 비정형적인 방대한 빅데이터이므로 적절한 피부상태 정보분석과 인공지능 기술을 접목한 지능화된 피부상태 진단 시스템이 필요하다. 본 논문에서는 소셜미디어의 텍스트 분석정보를 학습데이터로 가공하여 고객의 피부상태를 지능적으로 진단 및 관리하기 위한 피부상태진단시스템 SCDIS를 개발하였다. SCDIS에서는 딥러닝 기계학습 방법인 인공신경망 기술을 사용하여 자동적으로 피부상태 유형을 진단하는 인공신경망 모델 AnnTFIDF을 빌드업하여 사용하였다. 인공신경망 모델 AnnTFIDF의 성능은 테스트샘플 데이터를 사용하여 분석되었으며, 피부상태 유형 진단 예측 값의 정확성은 약 95%의 높은 성능을 나타내었다. 본 논문의 실험 및 성능분석결과를 통하여 SCDIS는 화장품 및 뷰티산업 분야의 피부상태 분석 및 진단 관리 과정에서 효율적으로 사용 가능한 지능화된 도구로 평가할 수 있다. 본 논문에서 제안된 시스템은 소셜미디어 기반의 새로운 환경에서 화장품 및 피부미용에 대한 사용자의 요구를 체계적으로 파악하고 진단하는 기초 기술로 사용 가능할 것이다. 그리고 이 연구는 새로운 기술 트렌드인 맞춤형 화장품제조와 소비자중심의 뷰티산업기술 수요를 해결하기 위한 기초 연구로 사용될 수 있을 것이다.

• 한국멀티미디어학회논문지
• /
• 제25권8호
• /
• pp.1038-1048
• /
• 2022

Ultrasound is one of the effective methods for skin treatment. The skin penetration depth of the ultrasound depends on the ultrasonic frequency, that is, when the ultrasonic frequency is high, the depth is shallow. We have developed a transducer which can generate effectively 3 different ultrasonic frequencies removing interference between 3 types of frequencies according to impedance matching technology. The generated powers of transducer are 40.67W at 3.MHz, 17.46W at 11.7 MHz, and 14.79W at 21.5 MHz. The signal interference between the three frequencies is designed so that they do not interfere with each other by separating the signals using the SPDT (Single Pole, Double Throw) switch. The developed hybrid ultrasound transducer can be applied in skin care or skin treatment and beauty therapy.

• 웰빙융합연구
• /
• 제4권4호
• /
• pp.15-25
• /
• 2021

Purpose: The purpose of this study is to form a new cosmetic market through the development of a composition with high skin permeability after adding omega 3 to Aloe Vera soothing gel products. Research design, data and methodology: In this study, omega-3 fatty acids were added to cosmetic products in the form of soothing gels. By applying nanoparticle technology to rapidly increase the penetration of raw materials into the skin, characteristics related to skin moisture and regeneration were determined. Omega-3 was used as a raw cosmetic material. Then 5% and 15% nanoparticle aqueous products containing omega-3 were prepared. The developed water hydrate was subjected to skin permeability test using artificial skin. Results: 53 hours of artificial transdermal penetration of the developed composition, the ethanol-based omega-3 containing nanoparticle solubilized raw material was about three times higher penetration than the ethanol-based omega-3 containing nanoparticle solubilized raw material. Conclusions: The raw material product (SR-1901) containing 5% of omega-3 nanoparticle water hydrate has skin regeneration ability and pain reduction effect. It can be expected that the skin cosmetics market will be reorganized into a new distribution structure and opportunity through omega-3 supplemented soothing gel cosmetics with improved efficacy than existing cosmetics.

• 대한의용생체공학회:학술대회논문집
• /
• 대한의용생체공학회 1996년도 춘계학술대회
• /
• pp.335-338
• /
• 1996

We attempted to reconstruct basement membrane (BM) in between the epidermal compartment and dermal compartment in the artificial skin preparation and examine its effect on the skin architecture as well as on the epidermal differentiation. Laminin, one of the component of BM, stimulate the migration of the basal cells but type IV collagen which is a major component of the mechanical network of BM did not stimulate epidermal migration. However laminin in the presence of type IV collagen at a 1:1 molar ratio did not stimulate epidermal migration but provide nice demarcation between epidermis and dermis. This mixture of laminin and type IV collagen enhanced epidermal differentiation in the artificial skin based on the morphological observation as well as biochemical criteria. The epidermal acquirement of migratory ability on the laminin-rich substrate suggest that this type of unbalance in the expression of the components of BM may prevail in the area of healing tissue and the invasive transition of the tumor. The result in this study provide the technical improvement in the artificial skin preparation and further application of this technique for the reconstruction of other bio-artificial organ.

• 한국컴퓨터정보학회논문지
• /
• 제27권9호
• /
• pp.191-203
• /
• 2022

화장품 및 뷰티산업에서 고객 맞춤형 제품과 서비스를 제공하는 것은 주요 기술 트렌드이고, 피부상태 진단과 관리는 중요한 필수기능이다. 고객의 요구 수준은 더욱더 높아지고 있으며 이에 대한 다양하고 섬세한 고민과 요구 사항이 소셜미디어 커뮤니티에서 활발하게 다루어지고 있다. 소셜미디어 상의 이미지는 매우 다양하고 비정형적이므로 피부상태 진단 및 관리에 필요한 체계적인 피부 이미지 식별을 위한 시스템이 필요하다. 본 논문에서는 소셜미디어 인스타그램에서 수집한 빅데이터로부터 피부 이미지 데이터를 지능적으로 식별하고, 피부상태 진단 및 관리를 위한 정형화된 피부 샘플 데이터를 추출하는 시스템을 개발하였다. 본 논문에서 제안한 시스템은 빅데이터수집분석단계, 피부이미지분석단계, 훈련데이터준비단계, 인공신경망훈련단계, 피부이미지식별단계로 구성된다. 빅데이터수집분석단계에서는 인스타그램으로부터 빅데이터를 수집하고 피부 상태 진단 및 관리를 위한 이미지 정보를 분석결과로 저장한다. 피부이미지분석단계에서는 전통적인 이미지 처리 기법을 사용하여 피부 이미지의 평가 및 분석 결과를 획득한다. 훈련데이터준비단계에서는 피부이미지 분석결과로부터 피부 샘플데이터를 추출하여 훈련데이터를 준비하였다. 그리고 인공신경망훈련단계에서는 이 훈련데이터를 사용하여 지능적으로 피부 이미지 유형을 예측하는 인공신경망 AnnSampleSkin을 단계별 고도화와 훈련을 통해 모델을 완성하였다. 피부이미지식별단계에서는 소셜미디어로부터 수집된 이미지에 대해 피부샘플을 추출하고, 훈련된 인공신경망 AnnSampleSkin의 이미지 유형 예측 결과들을 통합하여 최종 피부 이미지 유형을 지능적으로 식별한다. 본 논문에서 제안된 피부이미지식별 방법은 약 92% 이상의 높은 피부 이미지 식별 정확도를 나타내고 있고, 정형화된 피부 샘플 이미지 빅데이터를 제공할 수 있게 되었다. 추출된 피부샘플 세트는 피부 상태를 진단하고 관리하는데 매우 효율적이고 유용한 정형화된 피부 이미지 데이터로 사용될 것으로 기대된다.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• 제29권3호
• /
• pp.227-232
• /
• 2007

The radial forearm fasciocutaneous flap(RFFF) is a well-known flap for the reconstruction of oral and maxillofacial defects. It was first described by Yang et al. in 1981 and Soutar et al. developed it for the reconstruction of intraoral defect. RFFF provides a reliable, thin, and pliable soft tissue/skin paddle that is amenable to sensate reconstruction. It also has a long vascular pedicle that can be anastomosed to any vessel in either the ipsilateral or contralateral neck. However, split thickness skin graft(STSG) is most commonly used to cover the donor site, and a variety of donor site complications have been reported, including delayed healing, swelling of the hand, persistent wrist stiffness, reduced hand strength, and partial loss of the graft with exposure of the forearm flexor tendon. Various methods for donor site repair in addition to STSG have been developed and practiced to minimize both functional and esthetic morbidity, such as direct closure, V-Y closure, full thickness skin graft, tissue expansion, acellular dermal graft. We got a good result of using artificial dermis($Terudermis^{(R)}$) and secondary STSG for the repair of RFFF donor site defect esthetically and report with a review of literature.

• 대한화장품학회지
• /
• 제26권1호
• /
• pp.17-40
• /
• 2000

Cytotoxicity assays using artificial skins have been proposed as in vitro alternatives to minimize animal ocular and dermal irritation testing. Accordingly, the responses of artificial skins to the well-characterized chemical irritants toluene, glutaraldehyde, and sodium lauryl sulfate (SLS), and the nonirritant polyethylene glycol were studied. The evaluation of the irritating and non-irritating test chemicals was also compared with the responses observed in human dermal fibroblasts and human epidermal keratinocytes grown in a monolayer culture. The responses monitored included an MTT mitochondrial functionality assay. In order to better understand the local mechanisms involved in skin damage and repair, the production of several mitogenic proinflammatory mediators, interleukin-l$\alpha$, 12-HETE, and 15-HETE, was also investigated. Dose-dependent increases in the levels of かIn and the HETEs were observed in the underlying medium of the skin systems exposed to the two skin irritants, glutaraldehyde and SLS. The results of the present study show that both human artificial skins can be used as efficient in vitro testing models for the evaluation of skin toxicity and for screening contact skin irritancy.