• Title/Summary/Keyword: Artificial saliva

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Glass-alumina Composites Prepared by Melt Infi1tration: III. In-vitro Fatigue Behavior (용융침투법으로 제조한 유리-알루미나 복합체: III. In-vitro 피로거동)

  • 이득용;이세종;박일석;장주웅;김병수
    • Journal of the Korean Ceramic Society
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    • v.40 no.7
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    • pp.662-666
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    • 2003
  • Hertzian cyclic fatigue properties of the alumina-glass dental ceramics were evaluated in exact in vitro environment at contact loads from 200 N to 1000 N and up to 10$\^$6/ cycles to investigate the indentation damage and strength degradation. At 200 N, no strength degradation and crack generation was observed up to 10$\^$6/ contact cycles. As load increased from 200 N to 1000 N, the drastic reduction in strength was found when the damage transition from ring to radial crack occurred. The extent of strength degradation was more pronounced in vitro environments probably due to chemical reaction of artificial saliva with glass phase through radial cracks introduced during large numbers of contacts.

The preventive effect of fluoride materials on the dental caries by dental polishing prior to fluoride application (불화물 도포 전 치면연마 시행여부에 따른 우식예방효과)

  • Cho, Min-Jung;Ha, Myung-Ok;Oh, Han-Na
    • Journal of Korean society of Dental Hygiene
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    • v.12 no.1
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    • pp.113-122
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    • 2012
  • Objectives : This study was carried out to investigate the caries resistant effect of fluoride by dental polishing prior to fluoride application in vitro. Methods : Artificial caries lesion was made on the surface of specimen enamel taken from cow's permanent cuspid on the part of labial surface after resin embedding and polishing. Artificial dental plaque was formed on the 72 dental specimen 25~45 VHN(Vickers Hardness Number) which were divided into three groups(fluoride varnish, APF gel, control) with dental polishing and without polishing respectively. Fluoride varnish and APF gel group with 20 second polishing or without polishing were immersed in the artificial saliva respectively. Control group with or without polishing were immersed in the artificial saliva. Results : 1. Significant difference was not found by fluoride varnish between polishing group and non polishing group (p>0.01). 1) polishing group. The changes of Vickers Hardness Number(VHN) were $14.49{\pm}13.73$. 2) non-polishing group. The changes of VHN were $11.67{\pm}5.39$. 2. Significant difference was not found by APF gel between polishing group and non polishing group (p>0.01). 1) polishing group. The changes of VHN were $8.48{\pm}8.37$. 2) non-polishing group. The changes of VHN were $5.32{\pm}2.59$. Conclusions : Showed no significant difference between polishing group and non-polishing group regardless of fluoride materials (fluoride varnish, APF gel).

Improvement in oral function after an oral exercise program including whole-body exercises

  • Seo, Su-Yeon;Choi, Yoon-Young;Lee, Kyeong-Hee;Jung, Eun-Seo
    • Journal of Korean society of Dental Hygiene
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    • v.21 no.1
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    • pp.5-16
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    • 2021
  • Objectives: To evaluate the impact of an oral exercise program including whole-body exercises on oral function in older people. Methods: The participants (aged ≥65 years) were divided into three groups: intervention group I (only oral exercise), intervention group II (oral exercise with whole-body exercises), and control group (no intervention). The oral health status, saliva flow rate, and oral muscle strength were evaluated. Analyses were performed to compare the three groups and identify the changes in the aforementioned parameters before and after the program. Results: The saliva flow rate significantly increased in intervention groups I and II after the program. Oral muscle strength evaluation using the Iow a oral performance instrument showed that the anterior tongue strength increased significantly in intervention group I; the posterior tongue strength and cheek strength also increased but not significantly. The anterior tongue, posterior tongue, and cheek strengths significantly increased in intervention group II. Conclusions: The oral exercise program including whole-body exercises showed positive effects on the saliva flow rate and oral strength. No significant differences were observed in the quality of life related to oral health.

Controlling Factors of Feed Intake and Salivary Secretion in Goats Fed on Dry Forage

  • Sunagawa, K.;Ooshiro, T.;Nakamura, N.;Nagamine, I.;Shiroma, S.;Shinjo, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.10
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    • pp.1414-1420
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    • 2005
  • The purpose of this research was to determine whether or not feeding induced hypovolemia (decreases in plasma volume) and decreases in plasma bicarbonate concentration caused by loss of $NaHCO_3$ from the blood, act to suppress feed intake and saliva secretion volumes during the initial stages of feeding in goats fed on dry forage. The animals were fed twice a day at 10:30 and at 16:00 for 2 h each time. Prior to the morning feeding, the collected saliva (3-5 kg) was infused into the rumen. During the morning 2 h feeding period (10:30 to 12:30), the animals were fed 2-3 kg of roughly crushed alfalfa hay cubes. At 16:00, the animals were fed again with 0.8 kg of alfalfa hay cubes, 200 g of commercial ground concentrate and 20 g of sodium bicarbonate. In order to compensate for water or $NaHCO_3$ lost through saliva during initial stages of feeding, a 3 h intravenous infusion (17-19 ml/min) of artificial mixed saliva (ASI) or mannitol solution (MI) was begun 1 h prior to the morning feeding and continued until the conclusion of the 2 h feeding period. The physiological state of the goats in the present experiment remained unchanged after parotid gland fistulation. Circulating plasma volume decreases caused by feeding (estimated by increases in plasma total protein concentration) were significantly suppressed by the ASI and MI treatments. During the first 1 h of the 2 h feeding period, plasma osmolality in the ASI treatment was the same as the NI (non-infusion control) treatment, while plasma osmolality in the MI treatment was significantly higher. In comparison to the NI treatment, cumulative feed intake levels for the duration of the 2 h feeding period in the ASI and MI treatments increased markedly by 56.6 and 88.3%, respectively. On the other hand, unilateral cumulative parotid saliva secretion volume following the termination of the 2 h feeding period in the ASI treatment was 50.7% higher than that in the NI treatment. MI treatment showed the same level as the NI treatment. The results of the present experiment proved that the humoral factors involved in the suppression of feeding and saliva secretion during the initial stages of feeding in goats fed on dry forage, are feeding induced hypovolemia and decrease in plasma $HCO_3^-$ concentration caused by loss of $NaHCO_3$ from the blood.

Carbohydrate-electrolyte drinks exhibit risks for human enamel surface loss

  • de Melo, Mary Anne Sampaio;Passos, Vanara Florencio;Lima, Juliana Paiva Marques;Santiago, Sergio Lima;Rodrigues, Lidiany Karla Azevedo
    • Restorative Dentistry and Endodontics
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    • v.41 no.4
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    • pp.246-254
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    • 2016
  • Objectives: The aim of this investigation was to give insights into the impact of carbohydrate-electrolyte drinks on the likely capacity of enamel surface dissolution and the influence of human saliva exposure as a biological protective factor. Materials and Methods: The pH, titratable acidity (TA) to pH 7.0, and buffer capacity (${\beta}$) of common beverages ingested by patients under physical activity were analyzed. Then, we randomly distributed 50 specimens of human enamel into 5 groups. Processed and natural coconut water served as controls for testing three carbohydrate-electrolyte drinks. In all specimens, we measured surface microhardness (Knoop hardness numbers) and enamel loss (profilometry, ${\mu}m$) for baseline and after simulated intake cycling exposure model. We also prepared areas of specimens to be exposed to human saliva overnight prior to the simulated intake cycling exposure. The cycles were performed by alternated immersions in beverages and artificial saliva. ANOVA two-way and Tukey HDS tests were used. Results: The range of pH, TA, and ${\beta}$ were 2.85 - 4.81, 8.33 - 46.66 mM/L and 3.48 - $10.25mM/L{\times}pH$, respectively. The highest capacity of enamel surface dissolution was found for commercially available sports drinks for all variables. Single time human saliva exposure failed to significantly promote protective effect for the acidic attack of beverages. Conclusions: In this study, carbohydrate-electrolyte drinks usually consumed during endurance training may have a greater capacity of dissolution of enamel surface depending on their physicochemical proprieties associated with pH and titratable acidity.

Effects of Artificial Stomach Fluid and Digestive Enzymes on the Aglycone Isoflavone Contents of Soybean and Black Bean (Rhynchosia Molubilis : Yak-Kong) (대두와 쥐눈이콩의 비배당체 이소플라본 함량에 대한 인공위액과 소화효소 처리효과)

  • 강순아;장기효;조윤희;홍경희;서지혜;조여원
    • Journal of Nutrition and Health
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    • v.36 no.1
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    • pp.32-39
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    • 2003
  • Phytoestrogens, especially soy-derived isoflavones, are receiving great scrutiny as a food supplement for preventing hormone dependent disease such as postmenopausal osteoporosis. Their beneficial effects are derived from aglycone form of isoflavones, such as daidzein, genistein or glycitein. In contrast to the common usage of soybean, black bean (Rhynchosia Molubilis : Yak-kong) has been used as a supplement for preventing postmenopausal osteoporosis in oriental medicine. To investigate the effects of the saliva, artificial stomach fluid, and digestive enzymes on the conversion of glycosidic isoflavone to aglycone form, soybean and black bean were extracted with 70% methanol and freeze-dried. The recovery yield of methanol extracts of black bean was 14.1% which was higher than that of soybean, 13.5%. In terms of total isoflavones, we routinely obtained larger amount of isoflavones from black bean than those from soybean. By incubating methanol extracts of soybean and black bean with IN HCI for 180 min, the proportions of aglycones relative to the total isoflavone were significantly increased (32.4% and 52.4%, respectively). In vitro conversion, digestive enzymes ($\beta$-glucosidase and $\alpha$-glucosidase) may hydrolyze glycosidic bond of isoflavone more effectively than saliva or artificial stomach fluid did. It seems to say that the activity of $\beta$-glucosidase was higher than those of $\alpha$-glucosidase. The rate of conversion of glucoside form to aglycone form in black bean and soybean was low in physiological condition (pH) tested, although the enzymatic hydrolysis of glucoside was active. These results demonstrated that the composition of aglycone in food may be the important factors in terms of the bioavailability of isoflavones. (Korean J Nutrition 36(1): 32-39, 2003)

An Intravenous Replenishment of Salivary Components and Dry Forage Intake in Freely Drinking Large-type Goats

  • Sunagawa, K.;Hashimoto, T.;Izuno, M.;Hashizume, N.;Okano, M.;Nagamine, I.;Hirata, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.4
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    • pp.538-546
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    • 2008
  • Large-type goats eating dry forage secreted large volumes of saliva which resulted in the loss of $NaHCO_3$ from the blood and decreased plasma volume (hypovolemia). This research investigated whether or not the loss of $NaHCO_3$ from the blood and hypovolemia brought about by dry forage feeding actually depresses feed intake in large-type goats under free drinking conditions. The present experiment consisted of three treatments (NI, ASI, MI). All treatments in this experiment were carried out under free drinking conditions. In the NI control (NI), a solution was not infused. In the ASI treatment, i.v. infusion of artificial saliva was initiated 2 h before feeding and was continued for a total of 3 h concluding 1 h after the commencement of the feeding perod. In the MI treatment, mannitol solution was infused to replenish only water lost from the blood in the form of saliva. The hematocrit and plasma total protein concentrations during feeding in the NI control were observed to be higher than pre-feeding levels. This indicated that dry forage feeding-induced hypovolemia was caused by the accelerated secretion of saliva during the initial stages of feeding in freely drinking large-type goats. Increases in hematocrit and plasma total protein concentrations due to dry forage feeding were significantly suppressed by the ASI treatment. While hematocrit during feeding in the MI treatment was significantly lower than the NI control, plasma total protein concentrations were not different. From these results, it is clear that the MI treatment was less effective than the ASI treatment in mitigating the decreases in plasma volume brought about by dry forage feeding. This indicates that plasma volume increased during dry forage feeding in the ASI treatment which inhibited production of angiotensin II in the blood. The ASI treatment lessened the levels of suppression on dry forage feeding, but the MI treatment had no effect on it under free drinking conditions. The results indicate that despite the free drinking conditions, increases in saliva secretion during the initial stages of dry forage feeding in large-type goats caused $NaHCO_3$ to be lost from the blood into the rumen which in turn caused a decrease in circulating plasma volume and resulted in activation of the renin-angiotensin system and thus feeding was suppressed.

Electrochemical Characteristics of Dental Implant in the Various Simulated Body Fluid and Artificial Saliva (다양한 유사체액과 인공타액에서 치과용 임플란트의 전기화학적 특성)

  • Kim, T.H.;Park, G.H.;Son, M.K.;Kim, W.G.;Jang, S.H.;Choe, H.C.
    • Journal of the Korean institute of surface engineering
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    • v.41 no.5
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    • pp.226-231
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    • 2008
  • Titanium and its alloy have been widely used in dental implant and orthopedic prostheses. Electrochemical characteristics of dental implant in the various simulated body fluids have been researched by using electrochemical methods. Ti-6Al-4V alloy implant was used for corrosion test in 0.9% NaCl, artificial saliva and simulated body fluids. The surface morphology was observed using scanning electron microscopy (SEM) and energy dispersive x-ray spectroscopy (EDX). The electrochemical stability was investigated using potentiosat (EG&G Co, 263A). The corrosion surface was observed using scanning electron microscopy (SEM). From the results of potentiodynamic test in various solution, the current density of implant tested in SBF and AS solution was lower than that of implant tested in 0.9% NaCl solution. From the results of passive film stability test, the variation of current density at constant 250 mV showed the consistent with time in the case of implant tested in SBF and AS solution, whereas, the current density at constant 250mV in the case of implant tested in 0.9% NaCl solution showed higher compared to SBF and AS solution as time increased. From the results of cyclic potentiodynamic test, the pitting potential and |$E_{pit}\;-\;E_{corr}$| of implant tested in SBF and AS solution were higher than those of implant tested in 0.9% NaCl solution.

THE EFFECT OF CPP-ACP PRE-TREATMENT ON BOND STRENGTH OF A SEALANT (CPP-ACP 적용이 치면열구전색제의 결합강도에 미치는 영향)

  • Kong, Eun-Kyung;Jung, Sang-Hyuk;Mah, Yon-Joo;Ahn, Byung-Duk;Jung, Young-Jung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.37 no.4
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    • pp.445-452
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    • 2010
  • Recently the effect of CPP-ACP on early caries remineralization and prevention has hashbeen extensively researched. However, there has been a lack of research on micro-shear bond strength of a sealant applied on a CPP-ACP treated surface. Therefore, the purpose of this study was to evaluate the effect of CPP-ACP on the micro-shear bond strength of a sealant bonded with 3 different bonding systems. From the sound human 3rd molars, the 150 specimens were prepared and randomly assigned to six groups. Group I: Immersion in artificial saliva for 2 weeks + 35% phosphoric acid Group II: Immersion in artificial saliva for 2 weeks + 35% phosphoric acid + dentin bonding agent Group III: Immersion in artificial saliva for 2 weeks + self-etching adhesive Group IV: CPP-ACP application for 2 weeks + 35% phosphoric acid Group V: CPP-ACP application for 2 weeks + 35% phosphoric acid + dentin bonding agent Group VI: CPP-ACP application for 2 weeks + self-etching adhesive Sealant was applied and the micro-shear bond strength was measured. From the result of this study, it can be assumed that the CPP-ACP pretreatment can weaken the bond strength of a sealant if the enamel surface is conditioned with self-etch adhesive.

Gustatory evoked potential induced by stimulation of solution in human

  • Min, Byung-Chan;Park, Se-Jin;Sakamoto, Kazuyoshi
    • Proceedings of the ESK Conference
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    • 1997.04a
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    • pp.17-25
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    • 1997
  • The evoked potentials for concentrations of solutions of the four qualities of tastes(i.e., sweet, salty, sour, and bitter tastes) were measured. The solution was applied to the chorda tympani nerve located on the left side of the tongue at 20mm from the tip and 15mm left from the center line. The evoked potentials were detected from Cz referred to A1(left lobe) with the ground at the Fpz position. The Maximum potential level and its latency were evaluated. The individual threshold level of concentration of the solutions of four tastes were measured. Artificial saliva was used as a control solution. The evoked positive potentials for four qualities of tastes (i.e., gustatory evokde potentials) were found to be around 150ms by averaging eight responses. The arbitrary concentration of the solutions were presented by the relative concentration, which was the ratio of the arbitrary concentration to the individual threshold level. The characteristic relations between the latency and the relative concentration ;and those between the potential level and the relative concentration were evaluated. These evalutions showed that (1) the latencies for salty and bitter tastes denoted the minimum values due to for the change of relative concentration, and that (2) the latency for sour taste decreased as the relative concentrations increased, while the latency for sweet taste denoted the inverse tendency, Sinificant differences between any two maximum potential levels were not recognized. A response latencies to sucrose were abolished after treatment of tongue by a sweet-suppressing agent.

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