• Title/Summary/Keyword: Artificial host

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An Ultrastructural Study on the Glochidium and Glochidial Encystment on the Host Fish (Glochidium larva 의 구조와 숙주어류에서의 피양형태에 관한 미세구조적 연구)

  • Jeong, Kye-Heon
    • The Korean Journal of Malacology
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    • v.5 no.1
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    • pp.1-9
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    • 1989
  • A scanning electron microscopic study on the glochidium and glchidial encystment of Anodonta grandis on the guppy was conducted. The shape of the glochidium is apparently triangular and its averge size is 0.45mm X0.4mm when closed, The two glochidial shell valves are of the same size, kept together by a ligament of 120${\mu}{\textrm}{m}$ in length and 7 ${\mu}{\textrm}{m}$ in width. Each of the glochidial shell valves has a 16 ${\mu}{\textrm}{m}$ long hook sitdded with many spines on the superior face. A large area to the apex of the valve surrounding the base of the hook is provided with numerous small spines which become progressively smaller towards the periphery of the area, The external surface of the glochidial shell valve is covered with numerous small processes showing successive change in the shape and the pattern of destribution by part. Besides the processes, there are a number of niches scattered all over the exterior surface. The glochidial shell valve has two layers. One is the outer thin membrane bearing the processes and the niches and the others is the inner layer bearing numerous holes which any accessory structure and 2.65 ${\mu}{\textrm}{m}$ in diameter, emerges from a canal located at center of ventral plate of the mamtle, A total of three types of the hair cells are observed. In present artificial infection of the glochidium to the guppy, it took about three to four hours to complete an early cysts, During the period of encystment, The epithelial cells of the host fish actively migrated toward the attached glochidium and covered it.

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Probiotic Potential of Enterococcus faecium Isolated from Chicken Cecum with Immunomodulating Activity and Promoting Longevity in Caenorhabditis elegans

  • Sim, Insuk;Park, Keun-Tae;Kwon, Gayeung;Koh, Jong-Ho;Lim, Young-Hee
    • Journal of Microbiology and Biotechnology
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    • v.28 no.6
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    • pp.883-892
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    • 2018
  • Probiotics, including Enterococcus faecium, confer a health benefit on the host. An Enterococcus strain was isolated from healthy chicken cecum, identified as E. faecium by 16S rDNA gene sequence analysis, and designated as E. faecium L11. To evaluate the potential of E. faecium L11 as a probiotic, the gastrointestinal tolerance, immunomodulatory activity, and lifespan extension properties of the strain were assayed. E. faecium L11 showed >66% and >62% survival in artificial gastric juice (0.3% pepsin, pH 2.5) and simulated small intestinal juice (0.5% bile salt and 0.1% pancreatin), respectively. Heat-killed E. faecium L11 significantly (p < 0.05) increased immune cell proliferation compared with controls, and stimulated the production of cytokines (IL-6 and $TNF-{\alpha}$) by activated macrophages obtained from ICR mice. In addition, E. faecium L11 showed a protective effect against Salmonella Typhimurium infection in Caenorhabditis elegans. In addition, feeding E. faecium L11 significantly (p < 0.05) extended the lifespan of C. elegans compared with the control. Furthermore, genes related to aging and host defense were upregulated in E. faecium L11-fed worms. In conclusion, E. faecium L11, which prolongs the lifespan of C. elegans, may be a potent probiotic supplement for livestock.

Growth of Chaga Mushroom (Inonotus obliquus) on Betula platyphylla var. japonica (자작나무시루뻔버섯(차가버섯)을 접종한 자작나무로부터 버섯의 생장)

  • Ka, Kang-Hyeon;Jeon, Sung-Min;Park, Hyun;Lee, Bong-Hun;Ryu, Sung-Ryul
    • The Korean Journal of Mycology
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    • v.45 no.3
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    • pp.241-245
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    • 2017
  • Chaga mushroom (Inonotus obliquus), which has invaluable medicinal uses, grows only on living trees. To date, it is still harvested from its natural habitat and is not cultivated artificially. We artificially cultivated chaga mushrooms by inoculating its sawdust spawns on Betula platyphylla var. japonica in 2007, and monitored mushroom growth on the inoculated trees for 9 years. The mushrooms grew less than 1 cm per year, with the largest mushroom growing up to 9 cm in the 9 years of study. There was no difference in the growth (diameter at breast height) of trees with viable and non-viable I. obliquus. In conclusion, artificial cultivation of chaga mushroom was successful. Our findings suggest that selection of large B. platyphylla var. japonica as host tree could lead to better I. obliquus productivity. Further improvements of the method are needed to increase the success rate of I. obliquus inoculation.

Overproduction of Streptomyces griseus Protease A and B Induces Morphological Changes in Streptomyces lividans

  • Chi, Won-Jae;Kim, Jung-Mee;Choi, Si-Sun;Kang, Dae-Kyung;Hong, Soon-Kwang
    • Journal of Microbiology and Biotechnology
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    • v.11 no.6
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    • pp.1077-1086
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    • 2001
  • The sprA and sprB gene encoding chymotrypsin-like proteases Streptomyces griseus protease A (SGPA) and Streptomyces griseus protease B (SGPB) and the sprT gene that encodes Streptomyces griseus trypsin (SGT) were cloned from Streptomyces griseus ATCC10137 and overexpressed in Streptomyces lividans TK24 as a heterologous host. The chymotrypsin activity of tole culture broth measured with the artificial chromogenic substrate , N-succinyl-ala-ala-pro-phe-p-nitroanilide, was 10, 14 and 14 units/mg in the transformants haboring the sprA, sprB and sprD genes, respectively. The growth of S. lividans reached the maximum cell mass after 4 days of culture, yet SGPA and SGPD production started in the stationary phase of cell growth and kept increasing for up to 10 days of culture in an R2YE medium. The trypsin activity of the culture broth measured with the artificial chromogenic substrate , N-${\alpha}$-benzoyl-DL- arginine-p-nitroanilide , was 16 units/mg and SGT production started in the stationary phase of cell growth and kept increasing for up to 10 days of culture in an R2YE medium. The introduction of the sprA gene into S, lividans TK24 triggered the biosynthesis of pigmented antibiotics, actinorhodin and undecylprodigiosin, and induced significant morphological changes in the colonies in Benedict, R2YE, and R1R2 media. In addition, the introduction of the sprT gene also induced morphological changes in the colony shape without affecting the antibiotic production, thereby implying that certain proteases would appear to play very important and specific roles in secondary-metabolites formation and morphological differentiation in Streptomyces.

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Systematic Singular Association for Group Behaviors of a Swarm System (스웜 시스템의 그룹 행동을 위한 조직화된 단일 연합법)

  • Jung, Hah-Min;Kim, Dong-Hun
    • Journal of the Korean Institute of Intelligent Systems
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    • v.19 no.3
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    • pp.355-362
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    • 2009
  • In this paper, we present a framework for managing group behaviors in multi-agent swarm systems. The framework explores the benefits by dynamic associations with the proposed artificial potential functions to realize complex swarming behaviors. A key development is the introduction of a set of flocking by dynamic association (DA) algorithms that effectively deal with a host of swarming issues such as cooperation for fast migration to a target, flexible and agile formation, and inter-agent collision avoidance. In particular, the DA algorithms employ a so-called systematic singular association (SSA) rule for fast migration to a target and compact formation through inter-agent interaction. The resulting algorithms enjoy two important interrelated benefits. First, the SSA rule greatly reduces time-consuming for migration and satisfies low possibility that agents may be lost. Secondly, the SSA is advantageous for practical implementations, since it considers for agents even the case that a target is blocked by obstacles. Extensive simulation presents to illustrate the viability and effectiveness of the proposed framework.

Application of Mycorrhizal Research to Agriculture and Forestry (균근연구(菌根硏究)의 농림업(農林業)에의 응용(應用))

  • Lee, Kyung Joon;Lee, Don Koo;Lee, Won Kyu;Koo, Chang Duck
    • Journal of Korean Society of Forest Science
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    • v.59 no.1
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    • pp.121-142
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    • 1983
  • Recently mycorrhizal research has been one of the most fast-growing research areas in modern plant science and microbiology. The application potential of mycorrhizal techniques to agriculture and forestry is enormous in view of the ubiquitous nature of mycorrhizae and known benefits of mycorrhizae to host plants. Unfortunately, very few scientists in Korea are currently involved in mycorrhizal research. When a team of American plant pathologists visited Korea in September 1982 to participate in the Korea-U.S.A. Joint Seminar on Forest Diseases and Insect Pests, they were surprised by the principal author's statement that there was no single research project on mycorrhizae sponsored by Korean government or any scientific institutions. The author initiated a few years ago a research project on the ecology of tree mycorrhizae with a foreign financial support. Major areas of interest were survey of ectomycorrhizae in relation to soil fertility, taxonomic distribution of mycorrhizae among woody plants, identification of ectomycorrhizal fungi, and growth response of woody plants to artificial inoculation. In spite of the enormous application potential of mycorrhizae to agronomic plants, the subject of mycorrhizae has not been recognized by Korean agronomists, foresters or pathologists. The purpose of this review rather written in Korean is to introduce the techniques of mycorrhizal research to Korean scientists and to urge them to participate in challenging new scientific field which might bring us a remarkable increase in crop productivity and tree growth through manipulation of this unique symbiosis. In this review, following topics were discussed in the same order: introduction; brief history of mycorrhizal research; morphology and classification of mycorrhizae; distribution of mycorrhizae in plant kingdom and in soil profile; physiology of mycorrhizae (functions, mineral nutrition, mycorrhizal formation); interaction of mycorrhizae with soil-born plant pathogens. mycorrhizae in nitrogen-fixing plants; application of mycorrhizal techniques to nursery practices (isolation, culture, inoculation, and response); prospect in the future.

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Distribution and Host Plants of Parasitic Weed Cuscuta pentagona Engelm. (기생식물 미국실새삼의 분포 및 기주식물상)

  • Hwang, Sunmin;Kil, Jihyon;Lee, Chang-Woo;Kim, Youngha
    • Korean Journal of Plant Resources
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    • v.26 no.2
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    • pp.289-302
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    • 2013
  • Surveys were conducted to study nationwide distribution and identify habitats and host plants of Cuscuta pentagona Engelm. as a parasite. The major habitat types were arable land near agricultural waterways (54.3%), open field near roadside (41.3%) and artificial grassland (4.3%). The investigated host plants of Cuscuta pentagona consist of total 130 taxa: 10 varieties, 1 subspecies, 119 species, 95 genera, and 37 families. Among them, 30 taxa were alien plants. As for the useful plants, there were 59 taxa of edible ones (45.4%), 54 taxa of medicinal ones (41.5%), 13 taxa of ornamental ones (10%), 13 taxa of industrial ones (10%), and 9 taxa of pasture ones (6.9%). On arable land, it caused damage particularly to the growth of agricultural crops such as Oryza sativa L. (Rice) and fruits such as Malus pumila Mill. (Apple) etc. Its growth and distribution may cause economic loss for crops because of the close location of the arable land.

A Performance Study on CPU-GPU Data Transfers of Unified Memory Device (통합메모리 장치에서 CPU-GPU 데이터 전송성능 연구)

  • Kwon, Oh-Kyoung;Gu, Gibeom
    • KIPS Transactions on Computer and Communication Systems
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    • v.11 no.5
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    • pp.133-138
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    • 2022
  • Recently, as GPU performance has improved in HPC and artificial intelligence, its use is becoming more common, but GPU programming is still a big obstacle in terms of productivity. In particular, due to the difficulty of managing host memory and GPU memory separately, research is being actively conducted in terms of convenience and performance, and various CPU-GPU memory transfer programming methods are suggested. Meanwhile, recently many SoC (System on a Chip) products such as Apple M1 and NVIDIA Tegra that bundle CPU, GPU, and integrated memory into one large silicon package are emerging. In this study, data between CPU and GPU devices are used in such an integrated memory device and performance-related research is conducted during transmission. It shows different characteristics from the existing environment in which the host memory and GPU memory in the CPU are separated. Here, we want to compare performance by CPU-GPU data transmission method in NVIDIA SoC chips, which are integrated memory devices, and NVIDIA SMX-based V100 GPU devices. For the experimental workload for performance comparison, a two-dimensional matrix transposition example frequently used in HPC applications was used. We analyzed the following performance factors: the difference in GPU kernel performance according to the CPU-GPU memory transfer method for each GPU device, the transfer performance difference between page-locked memory and pageable memory, overall performance comparison, and performance comparison by workload size. Through this experiment, it was confirmed that the NVIDIA Xavier can maximize the benefits of integrated memory in the SoC chip by supporting I/O cache consistency.

(Design of data mining IDS for new intrusion pattern) (새로운 침입 패턴을 위한 데이터 마이닝 침입 탐지 시스템 설계)

  • 편석범;정종근;이윤배
    • Journal of the Institute of Electronics Engineers of Korea TE
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    • v.39 no.1
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    • pp.77-82
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    • 2002
  • IDS has been studied mainly in the field of the detection decision and collecting of audit data. The detection decision should decide whether successive behaviors are intrusions or not , the collecting of audit data needs ability that collects precisely data for intrusion decision. Artificial methods such as rule based system and neural network are recently introduced in order to solve this problem. However, these methods have simple host structures and defects that can't detect changed new intrusion patterns. So, we propose the method using data mining that can retrieve and estimate the patterns and retrieval of user's behavior in the distributed different hosts.

Development of an EMG-based Wireless and Wearable Computer Interlace (근전도기반의 무선 착용형 컴퓨터 인터페이스 개발)

  • Han, Hyo-Nyoung;Choi, Chang-Mok;Lee, Yun-Joo;Ha, Sung-Do;Kim, Jung
    • 한국HCI학회:학술대회논문집
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    • 2008.02a
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    • pp.240-244
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    • 2008
  • This paper presents an EMG-based wireless and wearable computer interface. The wearable device contains 4 channel EMG sensors and is able to acquire EMG signals using signal processing. Obtained signals are transmitted to a host computer through wireless communication. EMG signals induced by the volitional movements are acquired from four sites in the lower limb to extract a user's intention and six classes of wrist movements are discriminated by employing an artificial neural network (ANN). This interface could provide an aid to the limb disabled to directly access to computers and network environments without conventional computer interface such as a keyboard and a mouse.

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