• Title/Summary/Keyword: Artificial culture

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Culture Conditions on the Early Growth of Hizikia fusiformis(Phaeophyta) (톳(Hizikia fusiformis)의 초기생장에 대한 배양조건)

  • 황은경;박찬선;손철현
    • Journal of Aquaculture
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    • v.10 no.2
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    • pp.199-211
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    • 1997
  • Effects of light and temperature on the early growth of Hizikia fusiformis were investigated for establishment of artificial seed production. Young germlings were cultured for 70 days under different culutre conditions : light intensities (500, 1, 000, 2, 000, 4, 000, 8, 000 lux), photoperiods (8 : 16, 12 : 12, 16 : 8, (L : D)) and temperatue (10, 15, 20, 25, $30^{\circ}C$). Growth of the germlings was facilitated in 2, 000~4, 000 lux of light intensities, 16 : 8 (L:D) of photoperiods and 15~$20^{\circ}C$ of temperature. Culture conditions were revealed for the mass culture of artificial seeds of effective early growth in Hizikia cultivation.

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DA-3711:A POTENT TISSUE-ENGINEERED ACTIVE INGREDIENTS FOR ANTI-AGING

  • Kim, B. M.;Lee, M.;Lee, J. H.;I. S. Doo;M. K. Son;S. H. Kang;Kim, W. B.;J. W. Kwon
    • Proceedings of the SCSK Conference
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    • 2003.09a
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    • pp.659-659
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    • 2003
  • In this study, we have investigated the potent anti-aging effect of DA-3711, a cosmetic ingredient derived from artificial skin culture. The artificial skin was originally developed as a skin replacement for the treatment of chronic skin wounds. To produce DA-3711, neonatal human fibroblasts were seeded into biocompatible collagen/chitosan/glycosaminoglycan (GAG) scaffolds and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with fetal bovine serum and nonessential amino acids. Analysis of the culture broth (DA-3711) showed that growth factors such as VEGF, TGF-$\beta$, KGF were present at significantly higher levels that in the culture broth of fibroblasts cultured in monolayer. The biological activity of DA-3711 was assessed by measuring in vitro cell proliferation and collagen synthesis of normal human fibroblasts. Fibroblasts treated with 10% DA-3711 showed a 2-fold higher proliferation and 2 to 4-fold higher collagen synthesis than untreated cells. DA-3711 also exhibited anti-oxidative effects, since cells under peroxide-induced oxidative stress showed a 30% higher viability in DA-3711-containing medium than in medium without DA-3711 addition. The results suggest that DA-3711 may have anti-aging effects by stimulating skin regeneration and protecting against oxidative stress.

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Effect of a co-culture of scenedesmus dimorphus and nitrifiers on advanced wastewater treatment capacity (Scenedesmus dimorphus와 질산화 박테리아의 공배양이 하수고도처리능에 미치는 영향)

  • Choi, Kyoung-Jin;Zhang, Shan;Lee, SeokMin;Joo, Sung-Jin;Hwang, Sun-Jin
    • Journal of Korean Society of Water and Wastewater
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    • v.28 no.6
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    • pp.691-698
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    • 2014
  • This study investigated the effect of a co-culture of Scenedesmus dimorphus and nitrifiers using artificial wastewater on the removal of ammonium, nitrate and phosphate in the advanced treatment. To test the synergistic effect of the co-culture, we compared the co-culture treatment with the cultures using S. dimorphus-only and nitrifiers-only treatment as controls. After 6 days of incubation, nitrate was removed only in the co-culture treatment and total amount of N removal was 1.3 times and 1.6 times higher in the co-culture treatment compared to those in the S. dimorphus- and nitrifiers-only treatments, respectively. In case of total amount of P, co-culture treatment removed 1.2 times and 12 times more P than the S. dimorphus -and nitrifiers-only conditions, respectively. This indicates that the co-culture improved removal rates for ammonium, nitrate, and phosphate. This further implies that there was no need for denitrification of nitrate and luxury uptake of P processes because nitrate and phosphate can be removed from the uptake by S. dimorphus. In addition, co-culture condition maintained high DO above 7 mg/L without artificial aeration, which is enough for nitrification, implying that co-culture has a potential to decrease or remove aeration cost in the wastewater treatment plants.

Instability in in vitro fruiting of Cordyceps militaris (In vitro에서 큰번데기 동충하초 자실체형성의 불안정성)

  • Shrestha, Bhushan;Park, Young-Jin;Han, Sang-Kuk;Choi, Sung-Keun;Sung, Jae-Mo
    • Journal of Mushroom
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    • v.2 no.3
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    • pp.140-144
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    • 2004
  • Traditionally, Cordyceps species have been used as a part of herbal medicine in Oriental countries, including Korea for internal health, vigor and to cure different diseases related to heart, lung etc. In recent years, research on artificial fruiting of some species of the genus Cordyceps including C. militaris has been carried out extensively because of their medicinal value. Instability observed in the in vitro fruiting of C. militaris is reported in the present study.

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Understanding the Foreign Tech-Trend of Artificial Skin by the Analysis of Patents (특허정보분석을 통한 해외 인공피부 기술동향)

  • 이상필;강종석;이영무
    • Membrane Journal
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    • v.14 no.2
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    • pp.85-98
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    • 2004
  • The situation of technology Predominance and the distribution of core technology were visually mapped thorough the investigation of technical trend during 20 years, which was provided with the analysis of patent information for the artificial skin. Therefore, it was expected that technology mapping by means of multilateral analysis method allowed a good grasp of current technology trend of the artificial skin and the subdivision into nation and a field of research presents the direction of R&D. In the early R&D stage of artificial skin, the preparation technology of filters implantable into the living body including nursing devices, bandages, and dressings or absorbent pads (A6IF-002/10) was on the rise in research field of artificial skin until 1980's. And then the materials technology for coating prostheses (A6l L-027/00)was leading the core technology of artificial skin. Also, Nowadays the fusion technology connected the material technology with the cultivation technology of undifferentiated human or animal cells/tissues including culture media (C l2N-005/00, C 12N-005/06) was highlighted all over the world.

Study on Controllability of Artificial Intelligence and Status of Global Regulations (인공지능 통제 가능성 고찰과 글로벌 규제 현황 연구)

  • MiKyung Chang
    • The Journal of the Convergence on Culture Technology
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    • v.10 no.2
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    • pp.447-452
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    • 2024
  • As the remarkable achievements of generative artificial intelligence technology become increasingly visible, the issue of 'controllability' in artificial intelligence is emerging as a prominent global keyword. This comes at a time when existential threats, such as the possibility of machines dominating humans, are being raised. Accordingly, this study aims to establish the groundwork for shaping a social public sphere by closely examining the concept of control, the current status, and the global landscape of artificial intelligence. It seeks to address the innovative changes anticipated in future society, with artificial intelligence technology at its core. The study aims to derive implications for preparing countermeasures against social problems and unpredictable variables that may arise from the evolution of artificial intelligence technology. It also aims to present guidelines and strategic insights for the establishment of government regulations. Furthermore, the study seeks to uncover implications for the formation of social public discourse.

FORMATION OF BASEMENT MEMBRANE AND STRATIFICATION OF RABBIT ORAL KERATINOCYTES CULTURED ON HUMAN ACELLULAR DERMAL MATRIX (인간 무세포성 진피기질 위에 배양한 가토 구강각화상피세포의 중충화와 기저막 형성에 관한 연구)

  • Kim, Yong-Deok;Ahn, Kang-Min;Yum, Hak-Yeol;Chung, Hun-Jong;Kim, Soung-Min;Jang, Jeong-Won;Sung, Mi-Ae;Park, Hee-Jung;Hwang, Soon-Jung;Lee, Jong-Ho
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.27 no.6
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    • pp.510-522
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    • 2005
  • To assess the clinical applicability of bio-artificial mucosa which was made with autologous oral keratinocytes and human acellular dermal matrix, the formation of basement membrane and stratification of oral keratinocytes were evaluated. Six New Zealand white rabbits (around 2kg in weight) were anesthetized and its buccal mucosa was harvested (1.0 $\times$ 0.5cm size). Oral keratinicytes were extracted and cultured primarily with the feeder layer of pretreated NIH J2 3T3 fibroblast. These confluent cells were innoculated on the human acellular dermal matrix and cultured in multiple layer by air-rafting method. After 3, 5, 7, 10, 14 days of culture, each cultured bio-artificial mucosa was investigated the number of epthelial layer of by H&E stain and toluidine blue stain. The immuhohistochemical methods were used to evaluate the cell division capacity, the formation of basement membrane, and it's property of specific cells (PCNA, cytokeratin 14, laminin). Transmission electromicroscopy was used for the attachment between cells and matrix with the number of hemidesmosome. In result, the numbers of layer of stratified growth of oral keratinocyte cultured on the human acellular dermal matrix and the number of hemidesomal attachment between epithelial cells and human acellular dermal matrix were similar to the layers of normal oral mucosa after 10 days of culture. The cell division rate, basement membrane formation and proliferation rate increased as culture period increased. With these results, bio-artificial mucosa with autologous oral epithelial cells cultured on the acellular dermal matrix had clinically adaptable properties after 10 days' culture and this new bio-artificial mucosa model with relatively short culture time can be expected clinical applicability.

An analysis of learning performance changes in spiking neural networks(SNN) (Spiking Neural Networks(SNN) 구조에서 뉴런의 개수와 학습량에 따른 학습 성능 변화 분석)

  • Kim, Yongjoo;Kim, Taeho
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.3
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    • pp.463-468
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    • 2020
  • Artificial intelligence researches are being applied and developed in various fields. In this paper, we build a neural network by using the method of implementing artificial intelligence in the form of spiking natural networks (SNN), the next-generation of artificial intelligence research, and analyze how the number of neurons in that neural networks affect the performance of the neural networks. We also analyze how the performance of neural networks changes while increasing the amount of neural network learning. The findings will help optimize SNN-based neural networks used in each field.

News Article Identification Methods with Fact-Checking Guideline on Artificial Intelligence & Bigdata

  • Kang, Jangmook;Lee, Sangwon
    • International Journal of Advanced Culture Technology
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    • v.9 no.3
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    • pp.352-359
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    • 2021
  • The purpose of this study is to design and build fake news discrimination systems and methods using fact-checking guidelines. In other words, the main content of this study is the system for identifying fake news using Artificial Intelligence -based Fact-checking guidelines. Specifically planned guidelines are needed to determine fake news that is prevalent these days, and the purpose of these guidelines is fact-checking. Identifying fake news immediately after seeing a huge amount of news is inefficient in handling and ineffective in handling. For this reason, we would like to design a fake news identification system using the fact-checking guidelines to create guidelines based on pattern analysis against fake news and real news data. The model will monitor the fact-checking guideline model modeled to determine the Fact-checking target within the news article and news articles shared on social networking service sites. Through this, the model is reflected in the fact-checking guideline model by analyzing news monitoring devices that select suspicious news articles based on their user responses. The core of this research model is a fake news identification device that determines the authenticity of this suspected news article. So, we propose news article identification methods with fact-checking guideline on Artificial Intelligence & Bigdata. This study will help news subscribers determine news that is unclear in its authenticity.

Artificial Neural Network Analysis for Prediction of Community Care Design Research in Spatial and Environmental Areas in Korea

  • Yumi, Jang;Jiyoung An;Jinkyung Paik
    • International Journal of Advanced Culture Technology
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    • v.11 no.2
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    • pp.249-255
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    • 2023
  • This study aims to empirically confirm the effect and impact of community care design research centered on domestic space and environment on health promotion, diagnosis treatment, disease management, rehabilitation, and mitigation through the year of publication and perspective. To this end, based on 1,227 space and environment design studies from 2,144 community care design research data conducted for about 20 years from 2002 to 2022, when care services began in earnest through the long-term care system for the elderly, SPSS 26.0 was used to create a 'Multi-layer Perceptron' artificial neural network structure model was predicted and neural network analysis was performed. Research Results First, as a result of checking studies in each field of health care by year, there is a significant difference with the number of studies related to health promotion being the highest. Second, the five perspectives are region, time, dimension, function, and content perspective. As a result of inputting these variables as independent variables and analyzing their importance in the artificial neural network, the function perspective had the most influence, followed by the region > content > dimension > time perspective.