Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

FORMATION OF BASEMENT MEMBRANE AND STRATIFICATION OF RABBIT ORAL KERATINOCYTES CULTURED ON HUMAN ACELLULAR DERMAL MATRIX

인간 무세포성 진피기질 위에 배양한 가토 구강각화상피세포의 중충화와 기저막 형성에 관한 연구

  • Kim, Yong-Deok (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Ahn, Kang-Min (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Yum, Hak-Yeol (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Chung, Hun-Jong (Department of Pediatrics, Chung-Ju Medical Hospital, Kon-Kuk University) ;
  • Kim, Soung-Min (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kang-Nung National University) ;
  • Jang, Jeong-Won (Department of Pharmacology, Yonsei School of Medicine) ;
  • Sung, Mi-Ae (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Park, Hee-Jung (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Hwang, Soon-Jung (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Lee, Jong-Ho (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University)
  • 김용덕 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 안강민 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 염학렬 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 정헌종 (건국대학교 의과대학 소아과) ;
  • 김성민 (강릉대학교 치과대학 구강악안면외과학교실) ;
  • 장정원 (연세대학교 의과대학 약리학교실) ;
  • 성미애 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 박희정 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 황순정 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 이종호 (서울대학교 치과대학 구강악안면외과학교실)
  • Published : 2005.12.31
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Abstract

To assess the clinical applicability of bio-artificial mucosa which was made with autologous oral keratinocytes and human acellular dermal matrix, the formation of basement membrane and stratification of oral keratinocytes were evaluated. Six New Zealand white rabbits (around 2kg in weight) were anesthetized and its buccal mucosa was harvested (1.0 $\times$ 0.5cm size). Oral keratinicytes were extracted and cultured primarily with the feeder layer of pretreated NIH J2 3T3 fibroblast. These confluent cells were innoculated on the human acellular dermal matrix and cultured in multiple layer by air-rafting method. After 3, 5, 7, 10, 14 days of culture, each cultured bio-artificial mucosa was investigated the number of epthelial layer of by H&E stain and toluidine blue stain. The immuhohistochemical methods were used to evaluate the cell division capacity, the formation of basement membrane, and it's property of specific cells (PCNA, cytokeratin 14, laminin). Transmission electromicroscopy was used for the attachment between cells and matrix with the number of hemidesmosome. In result, the numbers of layer of stratified growth of oral keratinocyte cultured on the human acellular dermal matrix and the number of hemidesomal attachment between epithelial cells and human acellular dermal matrix were similar to the layers of normal oral mucosa after 10 days of culture. The cell division rate, basement membrane formation and proliferation rate increased as culture period increased. With these results, bio-artificial mucosa with autologous oral epithelial cells cultured on the acellular dermal matrix had clinically adaptable properties after 10 days' culture and this new bio-artificial mucosa model with relatively short culture time can be expected clinical applicability.

Keywords

References

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