• 한국균학회지
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• 제35권2호
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• pp.76-80
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• 2007

잎새버섯의 우량계통을 선발하여 농가에 보급하기 위하여 수집균주들의 균사체 생육 최적조건을 검토한 결과는 다음과 같다. 잎새버섯 수집균주의 생장에 적합한 배지는 PDA배지가 선발되었으며, 균사배양 적온은 $25^{\circ}C$로 나타났다. 잎새버섯의 균사생장에 적합한 수소이온 농도는 pH $4{\sim}5$로 약산성에서 균사생장이 우수하였다. 균사생장에 적합한 탄소원으로는 glucose와 fructose, 질소원으로는 peptone이 우수하였으며 C/N율은 $10{\sim}20$으로 질소 요구도가 다소 높은 것으로 나타났다.

• 한국기계기술학회지
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• 제13권1호
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• pp.31-40
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• 2011

This study was conducted to research how UCD(Ultra Constant Discharge) lamp lighting affects plant cultivation. To get the results within a short time, lettuce seedling was selected as a plant and nutrient hydroponic beds were used in a dark room for 100% artificial lighting. Two beds were installed for same distance lighting condition with UCD lamp and Hi-pressure Sodium lamp each and another two beds were installed for same light intensity condition with each type of lamp. After 15 days of cultivation, for both conditions, the yields under UCD lamp were weighed more than that under Hi-pressure Sodium lamp. The result can be analyzed that the spectrum of UCD lamp having near sunlight characteristic is much superior to that of Hi-pressure Sodium lamp having a line spectrum characteristic on yellow and red wavelengths for plant cultivation.

• 생물환경조절학회지
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• 제23권3호
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• pp.256-261
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• 2014

본 연구는 참다래 비가림 시설재배에서 인공수분이 착과 및 과실품질에 미치는 영향과 안정적인 착과를 위한 유효수분기간을 확인하고자 수행하였다. 또한 최근 참다래 재배농가들에서 많이 수행되고 있는 현탁액을 이용한 인공수분의 효과를 확인하고자 하였다. 인공수분은 비가림 시설재배에서뿐 아니라 노지재배에서도 참다래의 착과량이 개선되었으며 중량증가와 당도향상의 과실품질 개선효과가 확인되었다. 유효수분기간 확인을 위하여 만개 후부터 매일 7일간 인공수분이 수행되었다. 과실의 착과율, 과실당 종자수, 그리고 과실생장량은 만개 후 4일까지 차이가 나타나지 않았으나 만개 후 5일부터는 분명하게 감소하였다. 결과적으로 참다래 비가림 시설재배에서 유효수분기간은 만개 후 4일 이내로 나타났다. 또한, 현탁액을 이용한 인공수분은 석송자를 이용한 것과 착과량과 과실품질의 차이를 나타내지 않았다. 따라서, 화분 현탁액을 이용한 인공수분은 참다래 재배에서 노동력을 절감할 수 있는 매우 효율적인 수분방법이 될 수 있을 것이다.

• Applied Biological Chemistry
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• 제50권1호
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• pp.1-5
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• 2007

새송이버섯의 biomass를 위한 최적조건을 규명한 결과, 균사체 spot 배양을 위한 최적 조건은 PDA배지를 사용하여 24$^{\circ}C$에서 18일 배양 시 최적인 것으로 판단하였으며, 새송이버섯 균사체 Biomass를 위한 bioreactor의 배양 최적조건은 PDMP배지를 사용하여 pH 5.5, 18$^{\circ}C$에서 27일 배양시 reactor의 반응은 최적의 상태를 나타낼 수 있을 것이라 판단되었다. 최적조건에서 bioreactor를 이용한 새송이버섯 균사체의 인공적인 대량 배양이 가능하였으며, bioreactor에서 대량 인공 배양된 균사체를 이용한 새송이버섯 재배 시, 자실체가 형성되어 새송이버섯의 대량생산을 위한 biomass가 가능하였다.

• Mycobiology
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• 제35권4호
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• pp.226-229
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• 2007

To produce fruiting bodies of Oudemansiella mucida, porcelain fungus, on the oak sawdust medium, additives suitable for the mycelial growth and fruiting body formation were screened. In general, the mycelial growth of the three strains of O. mucida used in this study have been good on oak sawdust mixed rice bran of $20{\sim}30%$. The mycelia incubated in potato dextrose broth for 7 days were inoculated on oak sawdust medium supplemented with various ratios of rice bran and incubated for 30 days at $25^{\circ}C$ in the dark condition until the mycelia of O. mucida fully colonized the media from top to bottom. Then, top surface of the media in the bottles were horizontally scratched with a spatula and filled with tap water for 3 hours. To induce the primordial formation of O. mucida, the bottles were transferred to the mushroom cultivating room under 12 hrs of light (350 lux) and dark condition with relative humidity of 95% at $17^{\circ}C$. The primordia of O. mucida were formed on the surface of oak sawdust media after 7 days of incubation. The mature fruiting bodies were observed 5 days after primordial formation. The fruiting bodies O. mucida were formed on oak sawdust medium mixed with 5 to 30% rice bran. However, abundant fruiting-bodies of O. mucida were produced in oak sawdust medium supplemented with 20% rice bran. This is the first report associated with an artificial fruiting body production of O. mucida in Korea.

• ALGAE
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• 제35권2호
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• pp.123-131
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• 2020

Sargassum macrocarpum is a rich source of anti-inflammatory compounds. Recently, one of the compounds, tuberatolide B, has been reported as a functional anti-inflammatory additive for foods and nutraceuticals. The artificial seeding, growth and maturation of S. macrocarpum were investigated from May 2018 to September 2019. Indoor culture experiments for induction of egg release were conducted at temperatures of 17, 20, 23, and 26℃ and irradiances of 0, 10, 20, 40, and 80 μmol photons m^-2 s^-1 under 14 : 10 h (L : D) photoperiod. Within a given treatment combination, higher temperatures and irradiance levels favoured the maturation of receptacles in S. macrocarpum. Using artificial temperature and irradiance control, thalli matured one month earlier than thalli in nature. Under natural condition, receptacle formation began in April, and the eggs were released in June and July. The release of eggs from the receptacles was promoted at 17-20℃ and 40-80 μmol photons m^-2 s^-1, and the fastest growth of germlings occuring at 15-17℃ and 40 μmol photons m^-2 s^-1. For mature thalli, 300 g wet-weight was sufficient to seed 100 m of seed string. Thalli grew to 10.5 ± 2.6 cm in length at a density of 6.7 ± 3.3 individuals m^-1 after 1 year of cultivation, from germination. This study demonstrates that it is possible to cultivate S. macrocarpum for the production of anti-inflammatory products.

• 한국인터넷방송통신학회논문지
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• 제15권5호
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• pp.193-199
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• 2015

요즈음, 온도 및 습도, 조도 센서를 이용하여서, 식물 재배 환경 정보를 자동으로 수집하고 생장환경을 관리하는 지능형 온실 시스템이 많이 연구되고 있다. 본 논문에서는 조도, 온도 및 습도를 예측해서 인터넷 기반에서, 식물 재배의 최적 환경 조건을 추론하는 모의실험을 하였다. IOT 환경에서, 식물 재배 조건을 온도, 습도, 조도를 아두이노 센서에 실시간으로 온실 관리자에게 전송해서, 식물 재배에 필요한 최적의 온도, 습도 조건 값보다 크거나 적으면 자동으로 SMS 경고 문자를 실시간으로 전송해 주기 때문에, 급격한 기후 변화 (눈,비, 폭염)조건일 경우라도 최적의 화초 재배조건을 관리 할 수 있다. 본 논문에서는 퍼지논리와 WEKA TOOL을 이용하여서, 같은 온대 식물이라도, 그 지역에 필요한 최적의 온도, 습도, 조도(일사량) 수치를 산출하는 모의실험을 하였다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.21-21
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• 2014

A edible mushroom, Hypsizygus marmoreus is commercially cultivated in Northeast Asia. Japan's annual production is 110,000ton or more. Since 2002, cultivation is expanded in Korea. To investigate the morphological, cultural and microscopic characteristics of Hypsizygus marmoreus, 109 isolates were collected from Korea and other countries. Clamp connection, chlamydospore and arthrospore were present in all tested isolates of H. marmoreus except HYM-002 and HYM-004. Also pilealtrama, gilltrama, basidia, basidiospore and cystidia of fruiting body were no difference among the isolates in the present investigation. Morphological characteristics of fruiting body was that color of pileus was brown and white, irregular as marble, the average size 12~22mm and stipes was $46{\sim}91{\times}6{\sim}10mm$. Isolates HYM-031, HYM-047 and HYM-109 formed grayish-brown pileus with a faint pattern. Molecular analysis with RAPD and ITS rDNA sequence analysis were also performed to check the genetic relationships among H. marmoreus isolates. Based on the RAPD analysis using the URP-PCR, all isolates of H. marmoreus were clustered into large 3 groups but more than 90% showed high similarity. In addition, morphological and geographical differences have been classified as an independent cluster. The brown and white strains enclosed in same cluster. So genetically no significance difference was observed between these two strains. ITS gene sequences of 16 selected isolates which were 640 bp long, were aligned and compared. The similarity in ITS sequence was 94.8 to 99.1% among tested isolates and the H. marmoreus isolates in GeneBank. In conclusion the tested isolates were H. marmoreus. Morphological and molecular observations proved that all tested isolates were belonging to H. marmoreus. For the stable artificial cultivation, composition of optimum media, mature period and light condition were established. Optimal formula of artificial cultivation medium was Douglas sawdust: corn cob: soybean meal: wheat bran = 40:30:15:15. In addition, 7% rice bran and 3% yellow sucrose was the most effective composition for spawn's liquid medium. For the maturation of the isolates was favorable for growing for 20 to 30 days at $25^{\circ}C$ and the LED lights in mixture of white and blue was good for growth period. For effective growth, the temperature, humidity and aeration control in every step was important.

• 한국균학회지
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• 제28권1호
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• pp.11-15
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• 2000

Phellinus pini는 진흙버섯속(Phellinus)에 속하는 버섯으로 진흙버섯속 버섯 중에서 항암저지율이 가장 높다고 알려져 있다(중국본초도감). 온도별 배양결과 $25^{\circ}C$에서 34 mm/14일(PDA배지)로 생육이 가장 좋았고 배지별 시험에서는 PDA 배지에서 30mm/10일, MCM 배지에서 28mm/10일로 나타났으며 pH $5{\sim}6$에서 균사생장이 양호하였다. 시험관을 이용한 톱밥배지 배양에서는 참나무톱밥+버드나무톱밥 +미강(4.5:4.5:1)과 참나무톱밥+소나무톱밥+미강(4.5:4.5:1)에서 균사생장이 가장 빨랐고 균사밀도는 참나무톱밥+미강(9:1)과 소나무톱밥+미강(9:1) 배지가 가장 높았다. 참나무원목에 접종한 결과 접종 60일 경과시 원목내 균사생장은 40 mm로 나타났으며 균사활착율은 26%정도였고 최초 발이 소요기간은 접종 후 110일 정도로 나타났다.

• 한국토양비료학회지
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• 제45권1호
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• pp.9-15
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• 2012

This study was performed to produce basic data for silty reclaimed tidal lands and to develop techniques of environmentally-friendly utilization in agricultural system. We chose the two sites in Saemangeum reclaimed tidal lands, one (Site I) has been treated with cultivating green manure and conducting the desalinization process through submergence since April, 2007 and the other (Site II) has been under natural condition without artificial treatment. In situ and ex situ physic-chemical properties were determined and comparisons were made for soil profiles examined at these two sites in April 2009. Surface soil of Site I had lower EC and higher field saturated hydraulic conductivity than those of Site II, uncultivated land. Especially, exchangeable sodium content was lowest in Site I Ap1 layer than in other layers. This is probably due to flooding desalination and green manure cultivation. Besides, Ap1 and A2 layers of soil profile in Site I showed brighter soil color and more root observation than those of Site II. This is probably due to green manure cultivation. By the large, for high cash upland crops and intensive agricultural use of silty reclaimed tidal land, site-specific soil ripening such as flooding desalination and green manure cultivation could be useful.