• Title/Summary/Keyword: Artificial cultivation

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Artificial Cultivation of Phellinus linteus (Phellinus linteus의 인공재배)

  • Song, Chi-Hyun;Moon, Hye-Yeon;Ryu, Chung-Hyun
    • The Korean Journal of Mycology
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    • v.25 no.2 s.81
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    • pp.130-132
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    • 1997
  • Artificial cultivation of Phellinus linteus was studied. The best yield (B.E. $9.4{\pm}1.6$) of basidiocarps including basidiospore was obtained using Quercus acutissima among the logs of Morus alba, Quercus acutissima, Alnus japonica, Castanea crenata. Optimum temperature and required periods for the basidiocarps formation were $25^{\circ}C$ and about 10 months, respectively.

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Studies on the mutant introduction and new practical artificial cultivation of Lyophyllum decastes (잿빛만가닥버섯의 변이체 유도와 실용적 인공재배에 대한 연구)

  • Lee, Dae-Jin;Lee, Won-Jung;Kuen, Mi-Sung;Jang, Ju-Won;Lee, Won-Jae;Her, Jeong-Ran;Choi, Chang-Hyun;Hwang, Cheol-Ho;Lee, Byung-Eui
    • Journal of Mushroom
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    • v.9 no.2
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    • pp.63-68
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    • 2011
  • Artificial cultivation of Lyopyllun decastes is costly because the high expense is required for soil covering and bark. Therefore, most farmers of Lyopyllun decastes have recognized it is difficult to cultivate it. The purpose of this study is to reduce the cost of cultivation of Lyopyllun decastes by applying new cultivation method and introducing mutation of fungi. In this study, a new method of practical artificial cultivation was developed through many experiments using fermented pine sawdust and wheat bran. In conclusion, the method of practical artificial cultivation of Lyopyllun decaste is simple and cost efficient because neither soil covering nor bark is required.

Artificial Cultivation and Antioxidant Activity of Korean Native Mushroom Pycnoporus coccineus (한국산 간버섯의 인공재배 및 항산화 활성 평가)

  • Lee, Y.K.;Kim, M.K.;Yun, B.S.;Seo, G.S.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.21 no.2
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    • pp.35-47
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    • 2019
  • The mycelial growth of P. coccineus strain was good in PDA and YMA, but mycelial growth was low in MEA. Light irradiation during the incubation period affected the pigment formation and density of mycelia. Mushroom of P. coccineus strain was able to produce fruiting bodies in both bottle and bag cultivation, and oak sawdust was found to be the most suitable substrate for spawn culture and cultivation. In artificial cultivation using sawdust medium, fruiting body was grown to the extent that visual observation was possible from the 15th day, and it formed about 5 days fast in the treatment group with low relative humidity. From 40 to 45 days of mushroom development, mature fruiting bodies could be harvested, and the lower relative humidity of the growing room favored mushroom development and growth. Antioxidant activity of fruiting bodies harvested from artificial cultivation showed that ABTS radical scavenging activity of bottle-cultivated and wild fruit bodies were shown at 505㎍/㎖ and 515㎍/㎖, respectively. However, fruiting bodies harvested in bag cultivation were high at 910㎍/㎖. As a result of DPPH radical scavenging activity, all extracts were found to be inactive, exhibiting IC50 value of more than 2,000㎍/㎖ concentration. The ethyl acetate extract of mushrooms obtained from bottle cultivation showed the highest activity with 1,550㎍/㎖ IC50 value. Methanol extract of wild fruit bodies had the highest ABTS radical scavenging activity at the same concentration (10mg/㎖).

Effects of honey bee (Apis mellifera L.) colony size on the pollination of greenhouse-cultivated watermelon (Citrullus lanatus L.) under forcing cultivation

  • Lee, Kyeong Yong;Yoon, Hyung Joo;Lim, Jeonghyeon;Ko, Hyeon-Jin
    • International Journal of Industrial Entomology and Biomaterials
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    • v.37 no.2
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    • pp.109-116
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    • 2018
  • We investigated the effects of honey bee (Apis mellifera L.) colony size on the pollination of greenhouse-cultivated watermelon grown under the forcing cultivation system. The highest pollination activity of bees was observed ($14.3{\pm}5.0$ honey bees/day) when the bee colony size was 10,000 followed by 7,500 and 5,000 honey bees. There was a positive correlation between the bee colony size and pollination activity (R = 0.262) but insignificant difference in fruit set with different honey bee colony sizes (88%-91%). Evaluation of physical properties revealed that the weight and shape of watermelon were also not significantly different among different colony sizes. However, larger the bee colony size, higher the number of seeds were fertilized and rate of seed fertilization (p > 0.05). Number of seeds and content of sugar were negatively correlated (R = -0.714). Fertilized seeds showed a significant increase in mealy flesh, which has a negative effect on fruit quality, compared with that of the unfertilized seeds. Overall, we found that a colony size of 5,000 honey bees was the most effective for the pollination of watermelon grown under forcing cultivation. A comparison of the effects of bee pollination with those of artificial pollination suggested that artificial pollination can be effectively replaced by bee pollination in the forcing cultivation of watermelon, because fruit set, weight, and shape by bee pollination were similar to those achieved by artificial pollination.

Studies on the Artificial Cultivation of Pleurotus cystidiosus O.K.Miller, the Abalone Mushroom (전복느타리버섯(Pleurotus cystidiosus O.K.Miller)의 인공재배에 관한 연구)

  • Jang, Kab-Yeul;Jhune, Chang-Sung;Shin, Chul-Woo;Park, Jung-Sik;Oh, Se-Jong;Choi, Sun-Gyu;Sung, Jae-Mo
    • The Korean Journal of Mycology
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    • v.31 no.3
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    • pp.200-205
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    • 2003
  • The study was carried out on the artificial cultivation of the abalone mushroom, Pleurotus cystidiosus O.K.Miller. The pine sawdust substrates with 20% rise bran were good for mycelial growth and high quantity of P. cystidiosus in the bottle cultivation. Moreover, the proper volume for bottle cultivation was 850 ml and the removal of spawn and surface layer of the medium before pin-heading was more efficient. The yields of P. cystidiosus were higher in sawdust substrates added calcium carbonate than those not added calcium carbonate. The volume of 3 kg polypropylene bag is good for yield and biological efficiency in bag cultivation of P. cystidiosus. Cotton wastes were proper substrates for bag cultivation. In the effect of different cultivation temperature, $28{\pm}2^{\circ}C$ cultivation temperature was good for for primordial formation after inoculation.

Development of Crop Cultivation Technique Using Environment-friendly Artificial Media Using Urban Waste (도시발생 폐기물을 저공해 열처리 생산한 환경친화형 인공배지의 작물재배이용기법 개발)

  • 김선주;양용석
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 1998.10a
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    • pp.490-499
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    • 1998
  • This study was initiated to investigate the applicability of sludge from water or waste water treatment on the crop cultivation. Sludge is generated in the process of water and wastewater treatment process in large quantity. The sludge can cause many environmental problems. we have a many available treatment methods of sludge However, these methods still shortcomings and are not. The composition of typical municipal sludge contains organics and inorganics. The organics components are normally burnt in high temperature and mainly inorganics components are left after thermal treatment process. For the production of artificial media, chabazite was used as additive, and the mixed material was thermally treated in the firing kiln at 800~1,10$0^{\circ}C$ for about 10 minutes. The physical and chemical characteristics of artificial media were analyzed and it showed that the artificial media could be used as a media for plants and soil conditioner for farmland. The concentrations of the toxic heavy metals in the media were lower then those in the soil quality standard for farmland. This study illustrated that the artificial media production process, and introduced how to produce it's possible application as a media for Plant growth.

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Effect of Artificial Pollination, Pollination Time, and Pollen Bulking Agent on Seed Formation and Fruit Quality in the Shelter Greenhouse Cultivation of Kiwifruit (비가림 시설재배에서 인공수분, 수분시기 및 화분증량제가 참다래의 종자형성과 과실특성에 미치는 영향)

  • Lim, Kyeong-Ho;Kim, Wol-Soo;Lee, Sang-Hyun
    • Journal of Bio-Environment Control
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    • v.23 no.3
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    • pp.256-261
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    • 2014
  • This study was carried out to determine the effect of the artificial pollination on fruit set, seed formation and fruit quality in kiwifruit and to confirm the effective pollination time for optimal fruit set in the shelter greenhouse cultivation. Also, the effect of artificial pollination practice by the pollen diluent solution was tested. By artificial pollination, fruit set, seed formation, and fruit quality was improved both in the open field condition and in the shelter greenhouse cultivation. In order to find the effective pollination period (EPP), artificial pollination was carried out every day until 7 days after full bloom. Number of seeds and fruit set rate and fruit growth of kiwifruit were no significantly different until 4 days after full bloom (DAFB), but, those of kiwifruit from 5 DAFB significantly decreased. Consequently, the effective pollination period of kiwifruit in the shelter greenhouse was 4 days after full bloom. Also, artificial pollination by the pollen diluent solution was showed the similar fruit set and fruit quality compare to that by lycopodium powder. Accordingly, artificial pollination by the pollen diluent solution should be very effective practice for labor saving in kiwifruit cultivation.

Cultural Characteristics and Artificial Cultivation of Edible Mushroom, Clitocybe maxima (흰깔대기버섯의 배양적 특성 및 인공재배에 관한 연구)

  • Kim, M.K.;Kim, D.U.;Kang, H.W.;Seo, G.S.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.20 no.1
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    • pp.35-47
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    • 2018
  • A edible mushroom, Clitocybe maxima (Lentinus giganteusis) commercially cultivated in China and Taiwan. However, the researches of cultivation and cultural characteristics were not reported in Korea. In this study, we conducted on cultural characteristics and artificial cultivation of C. maxima. Six isolates were collected from China(3 isolates, commercial strain), Taiwan(1 isolate, commercial strain) and Korea(2 isolates, wild type). C. maxima and L. giganteus collected in China and Taiwan, respectively, are the same in China and are estimated to be of the same species as cultured characteristics. The mycelial growth of the collected strains was not significantly different in agar medium but it showed the best growth in YPMG in liquid culture. Optimum temperature for mycelial growth and induction of fruit body were 25℃ and 30℃, respectively. In order to artificial cultivation of C. maxima, cultural characteristics and artificial cultivation were carried out using agricultural by-products and forestry by-products materials. Mycelial growth was suitable in rice straw, cottonwood sawdust, corncob and rice seed medium, and it was selected as a cultivation medium. The suitable medium for artificial cultivation of C. maxima was selected to mixed medium 2(compounding ratio(v/v): 55% of hardwood sawdust, 5% of cottonseed pellets, 10% of cottonseed, 15% of beet pulp, 15% of swollen rice husks). It took about 30 days to be able to harvest, it was faster than oyster mushrooms. The cultivation period was about 30days. A isolate, CMA-002 was not initiation to fruit body primordiuma on the used cultivation substrate. Other 5 isolates were initiate and development to fruit body on the substrate used in this study. The strain CMA-003 was initiated to be fruiting body by 8~10 days after induction of fruiting body in all of the substrates. Isolate CMA-003 was generate to a bundle fruit body. Other isolates, however, were form fruit body individually. The CMA-003 strain was likely highly recommendable strains for farming. The optimum conditions for the induction and growth of C. maxima fruit body were 25~30℃, 8 hr illumination per day with white fluorescent lamp, 90~95% relative humidity, and 1,500 ppm of CO2 concentration in a cultivation room.

The beginning and history of Pleurotus spp. cultivation (느타리버섯 재배의 기원과 역사에 대한 고찰)

  • Jang, Gap-Yeol;Jeon, Chang-Seong;Gong, Won-Sik;Yu, Yeong-Bok;Kim, Gyu-Hyeon;Seong, Jae-Mo
    • Journal of Mushroom
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    • v.6 no.3_4
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    • pp.103-110
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    • 2008
  • A cultivation of Pleurotus spp. on tree-stumps and logs, was first described at the beginning of the twentieth century. Now Pleurolus spp. is the most popular mushrooms in Korea. Especially, P. ostreatus has been cultivated for many years since artificial cultivation methods were developed in 1980's. Its productivity occurring almost 32% in Korea. To find the vision of mushroom research, the beginning and history of Pleurolus spp. cultivation were introduced worldwide based on Journal of Mushroom Science. The important innovation and history in cultivation were also discussed in Korea.

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