• 미생물학회지
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• 제55권2호
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• pp.164-166
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• 2019

이 연구에서는 Microbacterium aurum KACC $15219^T$ (=IFO $15204^T$ = DSM $8600^T$)의 완전한 유전체 서열이 해독되었다. 하나의 원형 염색체는 3.42 Mbp였으며 G+C 함량이 69.9%였다. 해당 염색체 염기서열을 주석화한 결과, 총 3,096개의 유전자 서열이 발견되었다. 16종 이상의 탄소원을 분해하는 것으로 알려진 M. aurum KACC $15219^T$에는 방향족 아미노산 합성 기질인 quinic acid를 비롯한 다양한 탄소원의 이용과 관련된 유전자가 존재하였다. M. aurum KACC $15219^T$의 유전체 정보는 이 미생물에 대한 이해를 높이고 산업적인 이용을 위한 기반이 될 것이다.

• KSBB Journal
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• 제5권2호
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• pp.191-194
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• 1990

Tytosine은 방향족 아미노산이고, L-DOPA는 orth위치에 수산화기를 하나 더 가지는 tyrosine유도체이다. L-DOPA는 효소에 의하여 tyrosine으로부터 전환되어 생성할 수 있는데 반응액에서 두 물질을 분리, 분석하는 것은 상당히 어렵다. 본 논문에서는 두 아미노산 혼합물을 UV/VIS spetrophotermeter를 이용하여 동정함으로써 두 물질이 구분되는 두 파장을 결정하였고 두 파장에서 혼합물의 흡광도를 측정함으로써 농도를 정량하는 분석 system을 확립하였다. 이 방법은 사료에 아무런 전처리가 필요없으므로 손쉽고 간단하게 분석에 사용할 수 있다.

• 한국미생물·생명공학회지
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• 제29권3호
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• pp.155-161
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• 2001

Lactrococcus sp. HY449균줄르 M17-glucose broth에 배양하여 배양 상등액으로부터 propanol-actone 침전 ion-exchange chromatography gel-filtration chromatography 및 reverse-phase chroamtography 등을 통하여 비활성 25,600,000 BU/mg 인 순수한 bacteriocin 을 정제하였다. 정제 과정 주에서 ion-exchange chromatography 단계에 서는 35.3%의회수율이 7.3%로 감소하였다. Reverse-Phase chromatography에선 3.3%의 회수율을 보였고 활성도는 413.5배로 증가하였다. Tricine-SDS 전기영도 결과 bacteriocin 은 단일 밴드로 나타났으며, N-말단 아미노산 서열 분석을 수행한 결과 $NH_2$-IIe-Leu-Pro-GIn로 확인되었다. 아미노산조정 분석결과를 바탕으로 분자량을 예측한 결과 본 bacteriocin은 32개의 아미노산으로 이루어져 있으며 분자량은 3.6kDa인 것으로 추정되었다.

• 한국염색가공학회지
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• 제6권3호
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• pp.60-66
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• 1994

With the view of studying on the dual adsorption mechanism of acid dyes in connection with the structural difference of silk fibroin, silk fiber and silk fibroin memberane were used for equilibrium dyeing at $60^{\circ}C$, $70^{\circ}C$, $80^{\circ}C$ and pH 3.2, pH 5.0. The dyes used were C.I.Acid Orange 7 and C.I.Acid Red 88 introduced aromatic hydrocabon into Acid Orange 7. From the adsorption isotherm experiment, the total uptake of dyes can be described by Langmuir sorption and Nernst partition. Nernst partition. Nernst partition coefficient $K_1$ decrease of crystalline regions and orientation. The saturation value S of Acid Red 88 were large than total amino group contents and it was attributed it hydrophobic bond. On the other hand, the standard afficity and enthalpy were increased with the in crease of hydrophobic part of dyes. Both $k_1$ and $K_2$ were decreased with the increase of pH, but $k_2$ were more effected than $K_1$.

• Journal of Microbiology and Biotechnology
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• 제24권10호
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• pp.1389-1396
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• 2014

The entire nucleotide sequence of the TKL1 gene encoding transketolase (TKL) in an erythritol-producing yeast of Candida magnoliae was determined by degenerate polymerase chain reaction and genome walking. Sequence analysis revealed an open reading frame of C. magnoliae TKL1 (CmTKL1) that spans 2,088 bp and encodes 696 amino acids, sharing 61.7% amino acid identity to Kluyveromyces lactis TKL. Functional analysis showed that CmTKL1 complemented a Saccharomyces cerevisiae tkl1 tkl2 double mutant for growth in the absence of aromatic amino acids and restored transketolase activity in this mutant. An enzyme activity assay and RT-PCR revealed that the expression of CmTKL1 is induced by fructose, $H_2O_2$, and KCl. The GenBank accession number for C. magnoliae TKL1 is KF751756.

• 한국작물학회지
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• 제57권2호
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• pp.188-194
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• 2012

옥수수 우량 교잡종 육성을 위한 자료로 활용하고자 국내 육성 옥수수 자식계통, $F_1$ 종실 및 $F_2$ 종실의 이화학적 특성과 지방산 및 아미노산 조성을 각각 분석 검토하였다. 1. $F_2$ 종실의 단백질 함량이 9.0%로 자식계통 11.4%와 $F_1$ 종실 10.9%에 비하여 유의하게 낮은 것으로 나타났다. 지방 함량은 $F_2$ 종실이 자식계통과 $F_1$ 종실보다 높았다. 회분은 자식계통, $F_1$ 종실, $F_2$ 종실간 조성변이에 차이가 없었다. 2. 자식계통, $F_1$ 종실, $F_2$ 종실 모두 linoleic acid의 조성비가 가장 높고 oleic acid, palmitic acid, stearic acid, linolenic acid의 순으로 지방산 조성비가 높은 것으로 나타났다. $F_2$ 종실의 포화지방산의 함량은 17.6%로 $F_1$ 종실과는 비슷하였으나, 자식계통보다는 낮았다. $F_2$ 종실의 불포화지방산의 함량은 82.4%로 $F_1$ 종실과는 비슷하였으나, 자식계통보다는 높았다. 3. $F_2$ 종실의 leucine의 함량이 자식계통 및 $F_1$ 종실보다 낮았다. 반면 $F_2$ 종실의 valine, serine, threonine, cysteine, aspartic acid, lysine, arginine, histidine 등의 함량이 자식계통 및 $F_1$ 종실보다 높았다. Alanine 등 나머지 아미노산은 자식계통, $F_1$ 종실, $F_2$ 종실간 조성변이가 비슷한 경향이었다. 4. 산성아미노산인 MMA(monoamino monocarboxylic acid)가 자식계통, $F_1$ 종실, $F_2$ 종실에서 그 조성 비율이 가장 높았으며 산성 아미노산인 DMA(diamino monocarboxylic acid), 방향족 아미노산인 AAA(aromatic amino acid) 순으로 조성비가 높았다.

• Archives of Pharmacal Research
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• 제19권2호
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• pp.160-162
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• 1996

The structural analysis of tolfenamic acid, 2-[(3-chloro-2-methylphenyl)-amino]benzoic acid, was performed by single crystal X-ray diffraction technique. The compound was recrystallized from a mixture of ether and toluene in triclinic, space group $P2_1/c, \;with\; \partial=3.914(1), \; b=22.\; 020(2), \; c=14.271(1)\;{\AA}, \beta.=94.68(1)^{\circ}, $ and Z=4. The calculated density is $1.418 g/cm^3$. The structure was solved by the direct method and refined by full matrix least-squares procedure to the final R value of 0.039 for 1773 independent reflections. In the molecule, carboxyl group at the anthranilic acid is coplanar to the phenyl ring. The dihedral angle between the two aromatic rings of the molecule is $44.2^{\circ}$ The molecules are dirnerized through the intermolecular hydrogen bonds at the carboxyl group in the crystal.

• Journal of Animal Science and Technology
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• 제63권5호
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• pp.1169-1181
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• 2021

We investigated the comparative physicochemical attributes, oxidative stability, and microbial characteristics of 28 days dry-aged meat in between boneless sirloin (gluteus medius) and bone-in T-bone steaks (infraspinatus) muscles from Korean Native Hanwoo Steer (KNHS). Results reveal that regardless of the muscles, dry-aging increased protein content and water-holding capacity (WHC) (p < 0.05). Meat from infraspinatus-aged muscle led to darker meat with higher pH values than un-aged meat (p < 0.05). However, fat content, CIE a^*, and CIE b^* remained unchanged in both muscles at aging. At aged meat, thiobarbituric acid reactive substances (TBARS) values from bone-in infraspinatus muscle was 2.5-fold higher than boneless gluteus medius muscle (p < 0.05). Dry-aging led to an increase in the contents of total unsaturated fatty acids (UFAs), monounsaturated fatty acids (MUFAs), and UFA/saturated fatty acids (SFA) in both muscles (p < 0.05). Furthermore, gluteus medius aged muscle concentrated with olic acid (C18:1) compared to infraspinatus aged muscle. Irrespective of the muscles, dry-aging enhanced the total free amino acids (FAAs) as well as tasty, and bitter amino acid contents whereas decreased the tasty/bitter amino acids (p < 0.05). Aromatic amino acid, tryptophan that converted to serotonin was 2-fold higher in boneless gluteus medius muscle than bone-in infraspinatus muscle at pre and post aging processes (p < 0.05). Aged Infraspinatus muscle increased total bacteria (p < 0.05) while no salmonella spp. was detected in both muscles. Taken together, our study confirms that 28 days dry-aging profiling the quality characteristics of boneless sirloin (gluteus medius) and bone-in T-bone steaks (infraspinatus) distinctly while gluteus medius aged steak performs better owing to oxidative stability and functional compounds than infraspinatus aged steak.

• 한국식품영양학회지
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• 제36권6호
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• pp.572-580
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• 2023

Hypertension is characterized by excessive renin-angiotensin system activity, leading to blood vessel constriction. Several synthetic compounds have been developed to inhibit renin and angiotensin-converting enzyme (ACE). These drugs often have adverse side effects, driving the exploration of plant protein-derived peptides as alternative or supplementary treatments. This study assessed the phenolic compound and amino acid content and the antioxidant and antihypertensive activity of 5 South Korean staple crops. Sorghum had the highest phenolic compound content and exhibited the highest antioxidant activity. Millet grains, particularly finger millet (38.86%), showed higher antihypertensive activity than red beans (14.42%) and sorghum (17.16%). Finger millet was found to contain a large proportion of branched-chain, aromatic, and sulfur-containing amino acids, which are associated with ACE inhibition. In particular, cysteine content was positively correlated with ACE inhibition in the crops tested (r=0.696, p<0.01). This study confirmed that the amino acid composition was more correlated with the antihypertensive activity of grains than the phenolic compound content. Finger millet mainly contained amino acids, which have higher ACE inhibitory activity, resulting in the strongest antihypertensive activity. These findings underscore the antihypertensive potential of select crops as plant-based food ingredients, offering insight into their biological functions.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.476-476
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• 2003