• 대한본초학회지
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• 제29권6호
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• pp.35-43
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• 2014

Objectives : Official Arisaematis Rhizoma is described only three species, Arisaema amurnse, Arisaema erubescens, and Arisaema heterophyllum, in national Pharmacopoeia. However, other Arisaema species, Arisaema ringens, Arisaema takesimense and Arisaema serratum, also have been distributed as an inauthentic Arisaematis Rhizoma in the herbal market. To develop a reliable molecular authentication method for Arisaematis Rhizoma in species level, we analyzed DNA barcode regions using six Arisaema species. Methods : Thirty-eight samples of six Arisaema plants species (A. amurense, A. amurense f. serratum, A. heterophyllum, A. takesimense, and A. serratum) were collected from different habitate and nucleotide sequences of DNA barcode regions (rDNA-ITS, matK, and rbcL gene) were analyzed after PCR amplification. The species-specific sequences and phylogenetic relations were estimated using entire sequences of three DNA barcodes based on the analysis of ClastalW and UPGMA, respectively. Results : The comparative analysis of DNA barcode sequences were revealed inter-species specific nucleotides to distinguish the medicinal plant of Arisaema Rhizoma in species levels excluding between A. amurense and its subspecies (A. amurense f. serratum) and A. takesimense and A. serratum, respectively. However, we obtained sequence differences enough to discriminate authentic and inauthentic Arisaematis Rhizoma. Therefore, we suggest that these SNP type molecular genetic markers were an reliable method avaliable to identify official herbal medicines. Conclusions : These marker nucleotides could be useful to identify the official herbal medicines by providing definitive information that can identify original medicinal plant and distinguish from inauthentic adulterants and substitutes.

• 한국자원식물학회지
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• 제28권4호
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• pp.371-381
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• 2015

The object of this study was to obtain single oral dose toxicity of Arisaema Rhizome (Arisaema amurense f. serratum (Nakai) Kitag) aqueous extracts. Arisaema Rhizome (Chunnamsong in Korean) is one of the most important folk remedy plants used in Asia. In the study, a 28-day rat oral gavage study has been conducted with the extracts from Arisaema Rhizome at dose of 1,250, 2,500 and 5,000 ㎎/㎏/day. The following endpoints were evaluated: clinical observations, body weight, gross and microscopic pathology, clinical chemistry, and hematology. Based on the analysis of these endpoints, it was estimated that NOEL (no observed effect level) for male rats and NOAEL (no observed adverse effect level) for female rats are 5000 ㎎/㎏/day of the water-extracts from Arisaema Rhizome.

• 식물분류학회지
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• 제36권3호
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• pp.209-216
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• 2006

다도해해상국립공원에 속한 거문도에서 천남성속, 두루미천남성절, 무늬천남성아절에 해당되는 1 신아종인 거문천남성(Arisaema thunbergii subsp. geomundoense S.C. Ko)을 기재 및 도해하였다. 화수부속체 기부의 비후부의 색깔이 진한 자색 또는 적자색인 것이 기본종인 무늬천남성(A. thunbergii supsp. thunbergii)과 다르고, 비후부에 주름이 있는 것이 일본에 자생하고 있는 비후부가 평활한 A. thunbergii supsp. urashima와 다르다.

• 생약학회지
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• 제2권4호
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• pp.163-172
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• 1971

This paper attempts to observe the histological structure of Korean Arisaema ringens $S_{CHOTT}{'s}$ rhizome, to identify it's constituent and to investigate the pharmacological action with it's alcohol extract. The results are : 1) The inner structure of rhizome on the transverse section is divided into external and internal tissues by the ring of intercellular secretary sac. Raphides of calcium oxalate contained in mucilage cell, collateral vascular bundle, are extremely similar to those contained in Pinellia ternata. 2) The organs of the pistillate Arisaema ringens are larger and more plentiful than those of the staminate Arisaema ringens. The sexual identification is easy in the flowering season. 3) The alkaloid is identified by Meyer reagent as white p.p.t. at pH 2 of sulfuric acid. 4) The saponin is indentified remarkably by means of foaming reaction, Lieberman-Burchard reaction and hemolytic reaction. 5) The effect of alcohol extract on the relaxation of the isolated intestine of the rabbit is remarkably shown at the concentration of $10^{-3}g/ml$. 6) The effect of alcohol extract on the isolated ractus muscle of the frog increases the constructive action of acetylcholine at the concentration of $10^{-3}g/ml$. 7) The effect of alcohol extract on the isolated heart movement of the frog is decreased remarkably at the concentration of $10^{-3}g/ml$. 8) The effect of alcohol extract on the blood pressure of the rabbit is decreased by an interavenous injection of $10^{-3}g/kg$.

• 한국자원식물학회지
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• 제31권1호
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• pp.37-51
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• 2018

국내에 분포하는 천남성속의 계통학적 유연관계와 한약재 천남성(Arisaematis Rhizoma)으로 사용되는 천남성 3종(둥근잎천남성, 두루미천남성, 일파산남성)에 대한 분류학적 특징을 분석하기 위하여 천남성속 식물에 대한 분자계통학적 연구를 수행하였다. 3개의 범용성 DNA 바코드(ITS, matK, rbcL) 염기서열을 이용하여 국내 분포 천남성속 8분류군과 중국에 분포하는 약전수재 종 1분류군을 포함하는 9종 50개 시료와 같은 과의 Dracunculus vulgaris를 군외군으로 하여 유연관계를 분석하였다. 3개의 개별 DNA 바코드 염기서열과 이들을 유합한 염기서열로 계통학적 유연관계를 분석한 결과, 천남성속의 9 분류군은 6개의 독립적인 분계조를 형성하며 구별되었으며(Clade I, 둥근잎천남성 및 천남성; Clade II, 점박이천남성 및 섬남성; Clade III, 큰천남성; Clade IV, 일파산남성; Clade V, 두루미천남성; Clade VI, 무늬천남성 및 거문천남성), 이들 6개의 분계조는 각각 Pedatisecta절, Sinarisaema절, 및 Tortuosa절로 분류되었다. 또한 이들 DNA 바코드 구간의 비교 결과는 천남성속 식물의 종 및 종 이하 분류 단위의 분류학적 재검토의 필요성에 대한 중요한 정보를 제공하였다. 하지만 대한민국약전에 수재되어 한약재로 사용가능한 3종의 천남성 기원종의 종내 분류학적 연관성이나 분자계통학적 특징은 확인되지 않았다.

• Archives of Pharmacal Research
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• 제18권4호
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• pp.293-294
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• 1995

• 대한한의학방제학회지
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• 제11권2호
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• pp.213-232
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• 2003

Objectives : This study was designed to establish a characteristic discrimination of internal and external morphological standard of original plants and herbal states in Arisaema amurense var. serratum $N_{AKAI}$ and A. amurense $M_{AXIM}.$. Methods : In this studies, the external-internal morphological standards were determined by using stereoscope and butanol series. Results : 1. In the external shape of original plant, Arisaema amurense var. serratum $N_{AKAI}$ has high stem, an oval-elliptic leaflet and a serrate leaf margin. But A. amurense $M_{AXIM}$ has a relatively low stem, an upside oval leaflet and no serrate leaf margin. 2. On the herbs character of original plant, Arisaema amurense var. serratum $N_{AKAI}$ is small in height and diameter, but A. amurense $M_{AXIM}$ is relatively large in height and diameter. 3. On the gathered herbs character of original plant, the section of Arisaema amurense var. serratum $N_{AKAI}$ is brightly white, easily spilt and strong scent. But the section of A. amurense $M_{AXIM}$ is thin yellowish brown, not easily spilt and weak scent. Also the grade of gathered herbs, both can be classified by diameter and external scent. 4. In the current herbs character, Korean and Sichuan Province products are irregullar shape and have no scent, Jilin Province products are regular shape. Also, Guangxi Province products are small size and concave in one side, Hebei Province products are irregular size and shape. 5. In internal shape of original plant, epidermal cell of Arisaema amurense var. serratum is very tight. With the except of micro difference in parenchyma cell of cortex, on the whole there are nearly no differences with A. amurense $M_{AXIM}.$ Also, samely in the internal shape, according to collecting sites, epidermis exhibits a lot layer in curving state and secreting duct is developed and vascular bundle and exists between parenchyma cell of cortex. Conclusion : In the future, additional study is needed to distinguish herb and effect between same genus-degree of relatedness.

• 한국한의학연구원논문집
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• 제12권2호통권17호
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• pp.63-73
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• 2006

In the original plant of Arisaematis Rhizomas, the features of external and internal shape of Arisaema amurense var. serratum $N_{AKAI}$ and A. amurense $_{MAXIM}$. are as follows. 1. In the external shape of original plant, Arisaema amurense var. serratum $N_{AKAI}$ has high stem, an oval-elliptic leaflet and a serrate leaf margin. But A. amurense $M_{AXIM}$ has a relatively low stem, an upside oval leaflet and no serrate leaf margin. 2. On the herbs character of original plant, Ansaema amurense var. serratum $N_{AKAI}$ is small in height and diameter, but A. amurense $M_{AXIM}$ is relatively large in height and diameter. 3. On the gathered herbs character of original plant, the section of Arisaema amurense var. serratum $N_{AKAI}$ is brightly white, easily spilt and strong scent. But the section of A. amurense $M_{AXIM}$ is thin yellowish brown, not easily spilt and weak scent. Also the grade of gathered herbs, both can be classified by diameter and external scent. 4. In the current herbs character, Korean and Sichuan Province products are irregullar shape and have no scent, Jilin Province products are regular shape. Also, Guangxi Province products are small size and concave in one side, Hebei Province products are irregular size and shape. 5. In internal shape of original plant, epidermal cell of Arisaema amurense var. serratum is very tight. With the except of micro difference in parenchyma cell of cortex, on the whole there are nearly no differences with A. amurense $M_{AXIM}$. Also, samely in the internal shape, according to collecting sites, epidermis exhibits a lot layer in curving state and secreting duct is developed and vascular bundle and exists between parenchyma cell of cortex. In the future, additional study is needed to distinguish herb and effect between same genus-degree of relatedness.

• BMB Reports
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• 제38권5호
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• pp.526-532
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• 2005

A lectin with in-vitro anticancer activity against established human cancer cell lines has been purified by affinity chromatography on asialofetuin-linked amino activated silica beads from the tubers of Arisaema tortuosum, popularly known as Himalayan Cobra lily, a monocot plant from the family Araceae. The bound Arisaema tortuosum lectin (ATL) was eluted with glycine-HCl buffer, pH 2.5. ATL was effectively inhibited by asialofetuin, a complex desialylated serum glycoprotein as well as by N-acetyl-D-lactosamine, a disaccharide. It gave a single band corresponding to a subunit molecular weight of 13.5 kDa in SDS-PAGE, pH 8.8 both under reducing and non reducing conditions. When subjected to gel-filtration on Biogel P-200, it was found to have a molecular weight of 54 kDa, suggesting a homotetramer structure, in which individual polypeptides are not bound to each other with disulfide bonds. ATL is a glycoprotein with 0.9% carbohydrate content, stable up to $55^{\circ}C$ and at pH 2 to 10. The lectin had no requirement for divalent metal ions i.e. $Ca^{2+}$ and $Mn^{2+}$ for its activity. However, as reported for other monocot lectins, ATL gave multiple bands in isoelectric focusing and Native PAGE, pH 8.3. The lectin was found to inhibit in vitro proliferation of human cancer cell lines HT29, SiHa and OVCAR-5.

• The Korean Journal of Ecology
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• 제21권1호
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• pp.27-33
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• 1998

Arisaema robustum, which has the ability to change sex, was studied in a temperate broadleaf forest of Sanseong-ri, Joongbu-myeon, Gwangju-gun, Kyonggi Province, Korea. \ulcornerThe study, carried out from 1993 to 1997, focused on population dynamics energy budget among organs, size distribution, mortality, the relationships between sex and size, seed production and germination rate. In terms of energy budget among the organs, the ratio of aboveground to belowground biomass was 36.6 : 63.4 in non-female plants, and 81.4 : 18.6 in female plants. Also, in female plants, the ration of leaf to sexual organ biomass was 39.5 : 41.9. Therefore, the belowground ratio of female plants was lower than that of non-female plants. Plants were classified into 8 levels relative to the amount of leaf area by $100cm^2$. The rates of the smallest and the largest classes were 49% and 1%, respectively, and population distribution by size was relatively stable. The mortality averaged 13.1% per year and decreased in inverse proportion to leaf size (6.6% in the smallest and 0.0% in the largest size classes). Leaf areas were $64.1{\pm}48.5cm^2$ in non-flowering plants, $232.1{\pm}123.9cm^2$ in males and $444.8{\pm}153.9cm^2$ in females. The increase rates of leaf area per year varied from 1.9% in plants changing from female tomale, to 152.4% in plants changing from non-flowering to female. But plants which remained female for 2 years showed a decrease of 34.7%. >From this result, it is thought that the female plants invest more energy to reproduction than to vegetative organs. The correlation coefficient (CC) value between plant size and the number of seeds produced (0.55) was larger than the CC value between plant size and total seed weight (0.73). That is, the larger the plant size, the heavier the seed produced. The germination rate increased along with seed weight, and it was 95% in plants which were over 60mg fresh weight/seed.