• Korean Journal of Veterinary Service
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• v.32 no.4
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• pp.335-341
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• 2009

Canine brucellosis produce abortions and infertility in dogs and is currently diagnosed by serological methods such as rapid slide agglutination test with 2-mercaptoethanol (2-ME RSAT) and immunochromatographic assay (ICA). Bacterial isolation is considered gold standard for Brucella diagnosis and the polymerase chain reaction (PCR) is an alternative method to bacterial isolation. A total of 36 whole blood samples were collected from dogs reared in area of Chuncheon and were subjected to serology (2-ME RSAT and ICA for B. canis, Rose Bengal test and C-ELISA for B. abortus), blood culture and 3 types of PCRs (BSCP31, 16s rRNA, and OMP-2). All blood samples were negative by serology and blood cultures. The BCSP31 and the OMP-2 PCR detected 5 samples were positive whereas the 16S rRNA PCR detected all samples were negative as serological methods and blood culture did. From the results observed in the present study, we conclude that 16S rRNA PCR could be used for direct PCR for canine blood samples.

• Korean Journal of Veterinary Service
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• v.31 no.3
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• pp.315-329
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• 2008

A total of 710 bovine serum samples which are composed 532 bovine serum samples showed negative reaction and 178 bovine serum samples showed positive reaction with tube agglutination test (TAT) from North area of Gyeong-nam, Korea were tested using all the 3 assays which are Rose-Bengal test(RBT), tube agglutination test (TAT) and enzyme-linked immunosorbent assay (ELISA, two types) and analyzed for evaluation of specificity, sensitivity, reproducibility and predictive value. In the comparison of serum antibody titer agglutination test, RBT showed almost agreement with TAT. In the comparison of TAT and two types of ELISA method, they showed difference in specificity and sensitivity about 5%. But there is no significant difference in detecting sensitivity between two types of ELISA method and TAT. In serologic tests for bovine brucellosis, the new assay ELISA would be a good candidate for serologic survey for bovine brucellosis in Korea because it is efficient in detecting many test samples quickly. But the serum agglutination tests (RBT, TAT) are more economical and easy assay for detection. In the test of comparison of antibody titer between first day of finding and 10 days after finding by TAT, there was no change in 55% (76/139) of positive cattle.

• Journal of Korean Society on Water Environment
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• v.33 no.2
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• pp.130-139
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• 2017

With the need to evaluate accuracy of real-time measurement of cyanobacterial fluorescence to determine cyanobacterial blooms, this research examined 357 paired data (2013-2016) comprising both microscopic toxic cyanobacterial cell counts and concurrent real-time cyanobacterial concentrations at 2 sites (YS1 and YS2) in Yeongsan river. The increase in real-time cyanobacterial concentration was closely associated with the exceedance of 5,000 cyanobacterial cells/ml (odds ratio [OR] 1.07, 95% confidence interval [CI] 1.03-1.12) and 10,000 cells/ml (OR 1.08, 95% CI 1.04-1.12) at YS2 site. The area under the receiver operating characteristic (ROC) curve for the real-time cyanobacterial measurement at the YS2 site was 0.93, which indicates the measurement provides a high accurate detection of cyanobacterial blooms. On the ROC curve, the early alert levels of real-time cyanobacteria ranging $16-23{\mu}g$ chl-a/L would produce acceptable sensitivity of 79% and specificities greater than 90%. The real-time fluorescence measurement was found to be an accurate indicator of cyanobacteria and can serve as a tool for detecting toxic cyanobacterial bloom events in Youngsan river.

• Spatial Information Research
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• v.3 no.2
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• pp.91-102
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• 1995

The purposes of this paper are to find ways to detect ecotone between two eco'||'&'||'not;systems, measure the width and size of ecotone around the Mt. Pukhansan National Park, and investigate environmental impacts, if any, on the forest ecosystem of the park by human activities. Normalized Difference Vegetation Index(NDVI) derived from TM data and the ana'||'&'||'not;lytical capabilities of GIS are used to investigate characteristics of the ecotone, or the impact zone, of the park. Major findings of the study can be summarized as follows: First, it was found that ecotone of the park could be identified from NDVI -distance curves deri"ed by a series of buffering op'||'&'||'not;erations. Second, NDVIs of all three years of the national park are about 14 percent higher than surrounding areas. Third, width of ecotone were found to be closely related to phenology, adjacent land use, environmental degradation, etc. Third, ecotone of the study area was nearly douvled during 1985-1993 period, which might be caused by heavy trampling of visitors. Thus it can be concluded that further studies are needed to find exact causes of the deterioration of plant communities of the ecotone of the park.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.68-73
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• 2005

Various techniques have been proposed for detection and tracking of targets in order to develop a real-world computer vision system, e.g., visual surveillance systems, intelligent transport systems (ITSs), and so forth. Especially, the idea of distributed vision system is required to realize these techniques in a wide-spread area. In this paper, we develop a ubiquitous vision system for location-awareness of multiple targets. Here, each vision sensor that the system is composed of can perform exact segmentation for a target by color and motion information, and visual tracking for multiple targets in real-time. We construct the ubiquitous vision system as the multiagent system by regarding each vision sensor as the agent (the vision agent). Therefore, we solve matching problem for the identity of a target as handover by protocol-based approach. We propose the identified contract net (ICN) protocol for the approach. The ICN protocol not only is independent of the number of vision agents but also doesn't need calibration between vision agents. Therefore, the ICN protocol raises speed, scalability, and modularity of the system. We adapt the ICN protocol in our ubiquitous vision system that we construct in order to make an experiment. Our ubiquitous vision system shows us reliable results and the ICN protocol is successfully operated through several experiments.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.2036-2041
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• 2005

The underwater multi-agent technology has many potential for the various activities related to ocean development/conservation in the near future. For example, in such fields as water pollution investigation, aquaculture control, or coral reef research, we feel a growing need for a system that realizes underwater continuous monitoring in the wide rang e. In this case, the target monitoring area will be sliced planar hierarchically toward the depth as monitoring layers, and many AUVs arranged on each layer track the given trajectory and gather various environmental information continuously, with communicating each other in the layer or with other layers. To realize those systems we need to develop AUV multi-agent technologies. So we are now building basic systems for basin experiment for the development of AUV multi-agent behavior. We must experience many situations and problems to be solved for the development of its elemental technologies by using real systems as well as our computer simulations. In this paper we introduce our concept of the experiment in the near future and the hardware/software design of our two types of handy AUVs and ultrasound ranging/communication system for that experiment. One AUV is designed using a 17inches-diameter glass sphere with DOS/V and RT-Linux based subsystems, which is intended to use not only in the basin but also in the calm real sea. The other AUV is designed for the basin experiment using a 7inches-diameter acrylic sphere with low-cost embedded system with SH-2 based subsystems. The basin experiment to verify the basic AUV facilities and ultrasound ranging for position detection was carried out.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.41
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• pp.46.1-46.9
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• 2019

Background: Oral squamous cell carcinoma (OSCC) constitutes a group of tumors that exhibit heterogeneous biology, histopathology, and clinical behaviors. Case presentation: A 73-year-old male had a whitish leukoplakia-like lesion around inflamed peri-implant area (#42, #43, and #44), and this lesion had transformed to OSCC within 3 years. He underwent mass resection, selective neck dissection, and reconstructive surgery. To detect any carcinogenesis progression, we examined the removed tumor tissue as well as the patient's preoperative and postoperative sera to identify causative oncogenic proteins using immunoprecipitation high-performance liquid chromatography (IP-HPLC). Conclusions: The protein expression levels of p53, E-cadherin, β-catenin, MMP-10, HER2, NRAS, Met, HER2, and ERb were significantly lower in the serum collected on postoperative day 10 than in the preoperative serum, and if these proteins are consistently not elevated in the serum 3 months after surgery compared with the preoperative serum, these proteins can be potential oncogenic proteins. However, we also found that the serum extracted 3 months after the operation had elevated levels of oncogenic proteins compared with that of the preoperative and 10-day postoperative serum indicating the possibility of tumor recurrence. At postoperative follow-up period, ipsilateral neck metastasis and second primary lesion were found and additional surgery was performed to the patient. IP-HPLC using the patient's serum shows the possibility of oncogenic protein detection. However, follow-up IP-HPLC data is needed to find out patient-specific prognostic factors.

• The Korea Journal of Herbology
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• v.31 no.3
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• pp.29-35
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• 2016

Objectives : Ijung-tang (IJT) is a traditional herbal formula and has been used to treat digestive diseases such as abdominal pain, vomiting, and diarrhea. IJT consists of four herbal medicines, Ginseng radix, Atractylodis rhizoma alba, Zingiberis rhizoma, and Glycyrrhizae radix et rhizoma, containing various bioactive compounds. Quality assesment of IJT preparations was performed by analytical method for determining marker compounds.Methods : Determination of seven marker compounds in IJT preparations was quantitatively conducted by high-performance liquid chromatography equipped with a diode-array detector. The marker compounds were separated on a reversed-phase C18 column and the analytical method was successfully validated. Chemometric analysis was performed to compare IJT water extracts and commercial IJT granules.Results : Limit of detection and limit of quantification values were in the ranges of 0.093-2.649 μg/mL and 0.283-8.027 μg/mL, respectively. Precisions were 0.30-3.87% within a day and 0.23-2.35% over three consecutive days. Recoveries of the marker compounds ranged from 87.35-107.05%, with relative standard deviation (RSD) values < 6.15%. Repeatabilities were < 1.20% and < 1.71% of RSD value for retention time and absolute peak area, respectively. The results from quantitative analysis showed that the quantities of seven marker compounds of IJT samples varied, as were found in principal component analysis and hierarchical clustering analysis.Conclusions : The analytical method developed in the present study was precise and reliable to simultaneously determine marker compounds of IJT. Therefore, it can be used for the quality assessment of IJT preparations.

• Imaging Science in Dentistry
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• v.46 no.3
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• pp.173-178
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• 2016

Purpose: Optical coherence tomography (OCT) has been investigated as a novel diagnostic imaging tool. The utilisation of this equipment has been evaluated through several studies in the field of dentistry. The aim of this preliminary study was to determine through basic experiments the effectiveness of OCT in implant dentistry. Materials and Methods: To assess detection ability, we captured OCT images of implants in each of the following situations: (1) implants covered with mucosae of various thicknesses that were harvested from the mandibles of pigs; (2) implants installed in the mandibles of pigs; and (3) implants with abutments and crowns fixed with temporary cement. The OCT images were captured before cementation, after cementation, and after removing the excess submucosal cement. Results: If the thickness of the mucosa covering the implant body was less than 1 mm, the images of the implants were clearly detected by OCT. In the implants were installed in pigs' mandibles, it was difficult to capture clear images of the implant and alveolar bone in most of the samples. Remnants of excess cement around the implants were visible in most samples that had a mucosa thickness of less than 3 mm. Conclusion: Currently, OCT imaging of implants is limited. Cement remnants at the submucosal area can be detected in some cases, which can be helpful in preventing peri-implant diseases. Still, though there are some restrictions to its application, OCT could have potential as an effective diagnostic instrument in the field of implant dentistry as well.

• The Journal of the Acoustical Society of Korea
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• v.25 no.3E
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• pp.115-122
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• 2006

The bistatic scattering of an incident wave by a hemi-spherically capped cylinder is of particular interest because it has rarely been studied until the present day. The configuration of a hemi-spherically capped cylinder is similar to naval underwater weapons (submarines, mines, torpedos, etc.), but which is not exactly the same. This paper describes a novel laboratory experiment aimed at direct measurement of bistatic scattering by a hemi-spherically capped cylinder. Bistatic scattering by a hemi-spherically capped cylinder was measured in an acoustic water tank (5m long, 5m wide, 5m deep) using a high frequency projector (120kHz) and hydrophone. Measurements of monostatic scattering were also made under the same conditions. The bistatic scattering pattern by a hemi-spherically capped cylinder was measured against the incident angles $(0^{\circ},\;15^{\circ},\;20^{\circ},\;30^{\circ},\;45^{\circ},\;60^{\circ},\;90^{\circ})$ in order to verify various scattering pattern characteristics by the change of incident angle. The results indicate that the bistatic scattering TS at a wide scattering angle is much stronger than the mono static scattering TS. In bistatic scattering, the forward scattering TS is significantly stronger than the backward scattering TS, and the forward scattering pattern is also broader. In case of seven incident angles, the maximum value of forward scattering TS is about 14dB stronger than that of backward scattering TS. It is also found that forward scattering varies with the incident angle of sound to a much less extent than backscattering, and it is not seriously affected by the incident angle. These features could be the advantages of using forward scattering for detecting underwater targets at long range and increasing detection area and probability.