• Ocean and Polar Research
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• 제29권2호
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• pp.187-191
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• 2007

In this study, antioxidant activities of acetone/dichloromethane and methanol extracts of Arctic seaweeds were investigated. The antioxidant properties of both extracts of arctic seaweed were evaluated using two different peroxynitrite tests, including scavenging power on authentic peroxynitrite and inhibitory activities on peroxynitrite generation from 3-morpholinosydnonimine (SIN-1) producing superoxide anion and nitric oxide simultaneously in vitro. At concentration of $10\;{\mu}g/ml$, the acetone/dichloromethane and methanol extracts of Odonthalia dentata exhibited 54.6 and 64.2% inhibition against peroxynitrite generation from SIN-1 while they exhibited 24.3 and 23.0% scavenging activities on authentic peroxynitrite, respectively. On the other hand, the acetone/dichloromethane extract of Polysiphonia stricta exhibited 61.8% inhibition only against the induced peroxynitrite from SIN-1. Furthermore, the crude extracts of Odonthalia dentata and Polysiphonia stricta were fractionated into n-hexane, 85% aq. MeOH, n-BuOH, and $H_2O$ fractions, successively, and only 85% aq. MeOH fraction exhibited the best inhibition.

• Ocean and Polar Research
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• 제31권2호
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• pp.213-218
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• 2009

We examined the intracellular antioxidative effects of 20 Arctic seaweed extracts in Raw 264.7 cells. Each seaweed species was subjected to extraction using acetone/dichloromethane and methanol, respectively, after which the extracts were combined and used as the test sample. The antioxidant ability of all 20 seaweeds extracts was evaluated using four different activity tests, including the degree of occurrence of intracellular reactive oxygen species (ROS), $ONOO^-$, and lipid peroxidation in Raw 264.7 cells, as well as the extent of oxidative damage of genomic DNA purified from Raw 264.7 cells. Crude extracts from Monostroma obscurum, Alaria esculnta, Laminaria digitata, Desmarestia aculeata, Chorda filum, Ptilota seriata, Phycidrys rubens, Devaleraea ramentacea and Palmaria palmata exhibited significant scavenging effects on the generation of intracellular ROS. Among them, Monostroma obscurum and Phycidrys rubens significantly inhibited membrane lipid peroxidation and DNA oxidation. Moreover, Phycidrys rubens exhibited scavenging effects on peroxynitrite generated from SIN-1.

• Ocean and Polar Research
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• 제26권2호
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• pp.121-132
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• 2004

Concentrations of Al, As, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Zn were determined in four arctic brown algae (Laminaria saccharina, L. digitata, Alaria esculenta, Desmarestia aculeata) in an attempt to examine for their metal accumulation capacity and also to assess their contamination levels. Macroalgae were collected from shallow subtidal waters (<20m) of Kongsfjorden (Kings Bay) on Spitsbergen during the period of the late July to early August 2003. Metal concentrations highly varied between sampling sites, species and tissue parts. Input of melt-water laden with terrigenous sediment particles seemed to have a large influence on baseline accumulations of some metals (Al, Fe, Mn, Pb etc.) in the macroalgae, causing a significant spatial variation. There were also significant concentration differences between the young and old tissue parts in L. saccharina, L. digitata and A. esculenta. While Al, Fe, Mn, Pb were higher in the perennial parts (stipes and holdfast below meristematic region), Cd and As concentrations were significantly higher in the young blades above the meristematic region. Zn and Cr, on the other hand, showed little differences between the tissue parts. The highest metal concentrations were found in D. aculeata, which seems to be due to its filamentous fine branches leading to high surface/volume ratios. The lowest concentrations were found in the two Laminaria spp., the blades of which are thicker than D. aculeata and A. esculenta. No distinct signs of contamination were detected in the brown algal species analyzed. Added to this, the results of the present studies suggest the potential utility of L. saccharina, L. digitata and A. esculenta as biomonitors for metal pollution monitoring in this area.