• 한국어병학회지
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• 제28권2호
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• pp.93-98
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• 2015

Full-length recombinant VP2 and VP3 proteins of aquabirnavirus isolated from olive flounder were expressed successfully in E. coli expression system. After rats were immunized with these proteins, antisera were used for in vitro and in vivo neutralization test. In in vitro test, VP2 antibody titers were higher than that of VP3. In in vivo assays, fish challenged with aquabirnavirus neutralized with VP2 antibody survived longer than other fish.

• Mass Spectrometry Letters
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• 제14권2호
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• pp.36-41
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• 2023

A simple method was developed to determine residual p-arsanilic acid (ASA), an organo-arsenic compound used as a feed additive, in aquatic products (eel, halibut, and shrimp) using EDTA-assisted solvent extraction and LC-MRM. The method was successfully validated in terms of specificity, linearity (coefficient of determination ≥ 0.995), accuracy (recovery or R, 72.72-78.73%), precision (the relative standard deviation of R, 2.08-6.98%), and sensitivity (the lower limit of quantitation, 5 ppb) according to CODEX guidelines (CAC-GL 71-2009). The use of EDTA in the extraction solvent and water with a suitable pH modifier as the reconstitution solvent may be the key factors for successful results. This is the first method that can be used for monitoring residual ASA in aquatic products using LC-MRM and could contribute to establishing a better aquatic product safety management system.

• Ocean and Polar Research
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• 제45권2호
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• pp.33-42
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• 2023

Dissolved oxygen (DO) is a crucial parameter for assessing environmental conditions in aquatic ecosystems. However, commercial in situ dataloggers for oxygen optodes can be relatively expensive and limited in their specifications. In this paper, we present a novel design for a DO datalogger system based on the control boards family with RP2040 MCU chipset. Our design includes two types of dataloggers: a simple logging system and a programmable system for sampling rates via magnetic switches underwater for divers. We provide detailed descriptions of the system, including the MicroPython source code and drawings to aid in construction. We also discuss the various applications of our DO datalogger system in monitoring dissolved oxygen concentration in coastal waters and assessing the benthic metabolism of aquatic ecosystems. Our DO datalogger system provides an affordable and flexible option for researchers to accurately monitor DO concentrations in aquatic environments, and thereby improve our understanding of these complex ecosystems.

• 한국농공학회지
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• 제39권3호
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• pp.64-71
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• 1997

Floating aquatic plant system using water hyacinth was applied to examine feasibility as a wastewater treatment system for the industrial complex in rural area. The wastewater from the industrial complex does not likely contain toxic pollutants because the industries which generate wastewater with toxic compounds are not allowed to move in. Pilot plant was installed at Baeksuk Nongkong Danzi in Chunahn-City, Chunchungnam - Do , and field study was performed during summer and fall of 1996. Hydraulic loading rate was 0. $0.19m^3/m^2$.day. The effluent concentration of DO was higher than influent, and it implies that 0.6m depth reactor was reaerated enough to increase DO level. The influent concentration of BOD varied significantly from less than 30 to 120mg/${\ell}$ during the study period, however, effluent concentrations were generally lower than the water quality standard and removal efficiency was up to 85%. The influent concentration of COD also showed wide variation from less than 40 to 160mg/${\ell}$ and effluent concentration was higher than water quality standard when influent concentration was over l00mg/${\ell}$. The influent concentrations of T-N and T-P were lower than water quality standard and no further treatment was required, and these compounds were also removed in the system. Although some improvement and refinement are still required, overall* the floating aquatic plant system was proved to be feasible to apply to treat wastewater from industrial complex in rural area.

• 한국어병학회지
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• 제32권1호
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• pp.37-43
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• 2019

전 세계적으로 양식 산업의 급속한 성장으로 인해 수산생물의 생산량 증가와 함께 질병발생이 증가함에 따라 수산용의약품의 사용량이 매년 증가하고 있다. 수산용의약품은 수산생물질병의 예방 및 치료에 필수적인 반면, 의약품의 오 남용으로 인해 항생제 내성균 증가 및 수산식품의 위생학적 안전성에 심각한 문제를 야기할 수 있다. 따라서 본 연구에서는 국내에 위치한 모든 수산질병관리원을 조사하고, 여기서 판매되는 수산용의약품을 지역별로 2012년과 2015년에 비교 조사하였다. 더불어 수산용의약품의 판매경로를 조사함으로서 수산식품안전성 강화를 위한 기초자료로서 활용이 가능하다. 나아가 향후 연구에서는 수산양식장에서 직접 사용하는 의약품의 양을 집계하여 본 연구 결과와 비교분석 후 효과적인 수산용의약품 관리체계를 마련해야할 것이다.

• 대한수의학회지
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• 제50권4호
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• pp.331-333
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• 2010

Moribund albino catfish, Clarias sp., displayed from an indoor private commercial aquarium were submitted in the laboratory for diagnostic examination. Dense culture of bacteria was recovered from the kidney and was characterized using Vitek System 2 and showed 98% probability to Aeromonas (A.) hydrophila. PCR result showed positive using A. hydrophila extracellular hemolysin gene ahh1 (130 bp) and aerolysin gene aerA (309 bp). The 16S rRNA gene was identical and exhibited 97% sequence similarity with the other known isolates of A. hydrophila available in the GenBank. In this paper, we reported the isolation and molecular detection of A. hydrophila from an albino catfish.

• 대한통합의학회지
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• 제7권3호
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• pp.141-148
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• 2019

Purpose: The present study investigated the effects of 12-week aquatic exercise training on isokinetic muscle function of the shoulder in adolescents with cerebral palsy. Methods: The study included four male and four female adolescents with cerebral palsy. Isokinetic muscle function was measured at an angular velocity of $60^{\circ}/s$, using Biodex System VI Pro. The peak torques of internal rotation and external rotation were measured before and after training. Aquatic exercise training was performed once a day for 120 min, 4 times a week for 12 weeks. Results: The peak torque of external rotation according to body weight and mean power of internal rotation were significantly higher after training (p < 0.05). Conclusion: Our findings suggest that 12-week aquatic training for adolescents with cerebral palsy can improve isokinetic muscle function of the shoulder. Future studies should analyze the changes in isokinetic muscle function of the shoulder in more detail using various aquatic exercise programs to investigate their effects on individuals with cerebral palsy.

• Fisheries and Aquatic Sciences
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• 제16권4호
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• pp.261-265
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• 2013

The study was conducted to investigate the responses of the hepatic microsomal cytochrome P450 monooxygenase system in the rock bream Oplegnathus fasciatus after chronic exposure to 0, 1, 2, 4, and $8{\mu}g/L$ tributyltin (TBT) concentrations for 4 weeks. Hepatic cytochrome 450 content and ethoxyresorufin O-deethylation (EROD) activity were found to significantly increase in fish treated with the higher concentration of TBT (${\geq}4{\mu}g/L$); however, no significant changes were observed in penthoxyresorufin O-deethylation (PROD) activity in all treated groups compared to the control group. These findings suggest that exposure to a low TBT concentration (${\geq}4{\mu}g/L$) has the potential to induce cytochrome 450 content and EROD enzyme activity in hepatic tissue in the rock bream.

• 한국환경과학회지
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• 제26권12호
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• pp.1341-1353
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• 2017

This study aimed to identify the aquatic and riparian factors associated with the presence/absence of the Eurasian otter in the Nakdong river basin, where the species is relatively more abundant than other otter species. Environmental factors and presence records were collected. Geographical Information System technology and chi-square test were used to compare environmental gradients in aquatic and riparian factors between presence and absence sites. Aquatic habitat attributes were evaluated with natural riverside sandbars and channel crossing artificial structures, the ratio of channel width to alluvial plain width, riverbed substrate, and flow diversity. Riverbank characteristics, bank materials, man-made embankment types, and land use/land cover of inland and riverside areas were selected as riparian habitat attributes. Compared to the aquatic attributes, riparian attributes were highly significant when assessing otter presence and absence sites, suggesting that conservation of suitable riparian areas to provide maternity and resting areas for otter species is essential in the Nakdong river basin. None of the aquatic attributes examined were statistically significant when evaluating otter presence or absence. These results indicate that the presence of suitable riparian area for resting and reproduction habitats is more critical to the presence of Eurasian otter than food availability in aquatic areas. To inform implementation of effective conservation actions, broad-scale factors, such as watershed attributes, would be needed to further assess habitat conditions of the Eurasian otter.

• 대한수의학회지
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• 제56권1호
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• pp.45-46
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• 2016

For several days, there was a series of mortalities of chub mackerel (Scomber japonicus) that were reared for public exhibition in a private aquarium in Seoul, Korea. As part of the diagnosis of the dead fish, a bacterial isolate from the kidney was cultured, identified, and confirmed to be Vibrio (V.) harveyi using Vitek System 2 and 16S rRNA gene sequencing. Phylogenetic analysis was also performed by the neighbor-joining method. As a result, the V. harveyi isolated from chub mackerels of a private aquarium in Korea, called as SNUVh-LW1, was clustered in the same group with V. harveyi ATCC33843.