• Food Science and Biotechnology
• /
• v.17 no.5
• /
• pp.925-930
• /
• 2008

Norovirus has become the most common cause of human gastroenteritis in developed countries. Detection procedures of foodborne viruses from foods require several steps. The concentration step using polyethylene glycol (PEG) is time-consuming and the detection efficiency of reverse transcription-polymerase chain reaction (RT-PCR) is affected by inhibitors from food components. In this study, a rapid detection method based on buoyant density centrifugation was developed to replace the time-consuming chloroform-polyethylene glycol-Tris Tween method. Feline calicivirus that belongs to the family Caliciviridae was used as a surrogate model for norovirus. After artificial inoculation of feline calcivirus (FCV) to oyster and lettuce, 830 ${\mu}L$ of homogenized sample suspension was layered on the top of 670 ${\mu}L$ 20% percoll and centrifuged. Then RNA extraction step was proceeded with the supernatant. By varying several physical conditions, the detection limits were lowered to $2.4{\times}10^2$ PFU per 1 g in oyster and $2.4{\times}10^0$ PFU per 1 g in lettuce. The protocol obtained in this study could be used to develop new detection method for norovirus in foods.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.38 no.6
• /
• pp.620-628
• /
• 2010

Small satellites can be used for various space research and scientific or educational purposes due to advantages in small size, low-cost, and rapid development. Small Satellites have many advantages of application to Responsive Space. Compared to traditional larger satellites, however, Small satellites have many constraints due to limitations in size. Therefore, it is difficult to expect high performance. To approach maximum capability with minimal size, weight, and cost, standard modular platform of Small satellites is necessary. Modularity supports plug-and-play architecture. The result is Small satellites that can be combined quickly and reliably using plug-and-play mechanisms. For communication between modules, standard bus interface is needed. Controller Area Network(CAN) protocol is considered optimum data bus for modular Small satellite. CAN can be applied to data communication with high reliability. Hence, design optimization and simplification can also be expected. For ease of assembly and integration, modular design can be considered. This paper proposes development method for standardized modular Small satellites, and describes design of data interface based on CAN and a method of testing for modularity.

• Archives of Plastic Surgery
• /
• v.35 no.3
• /
• pp.273-278
• /
• 2008

Purpose: The most widely accepted protocol for alveolar cleft reconstruction is to repair it during the mixed dentition stage. There were lower resorption rate (about 88%) at this stage. However we found some cases that need repeated bone grafting. Therefore we sought to analyze the cause of repeated alveolar bone grafting in connection with other factors. Methods: From January 2000 to January 2006, thirty-nine secondary alveolar bone grafts with iliac crest spongiosa were carried out. In 39 patients, 5 patients who had significant bone graft resorption received repeated alveolar bone graft. In all the cases, the causes of repeated bone grafts were dental root exposure(angulation), and the deficiency of the bony support for lateral incisor or canine eruption. In 3 cases, there was deficiency of the alveolar bone at the cleft side. There was the need of repeated bone grafts for orthodontic treatment in 2 cases and for application of dental implants in 1 case. Results: During the follow-up period, the clinical and radiologic examinations showed that repeated alveolar bone grafts were maintained successfully without any complications. The volume of the repeated bone graft was sufficient for orthodontic treatment and implantation. Conclusion: The essential conditions for successful alveolar bone grafting includes the status of cleft sided teeth, further treatment and planed schedule, as well as canine eruption. Alveolar bone grafting has to be performed with difference of each case in mind.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
• /
• 2004.11a
• /
• pp.371-374
• /
• 2004

Induction motors are a critical component of many industrial processes and are frequently integrated in commercially available equipment. Safety, reliability, efficiency, and performance are some of the major concerns of induction motor applications. Preventive maintenance of induction motors has been a topic great interest to industry because of their wide range application of industry. Since the use of mechanical sensors, such as vibration probes, strain gauges, and accelerometers is often impractical, the motor current signature analysis (MACA) techniques have gained murk popularity as diagnostic tool. Fault tolerant control (FTC) strives to make the system stable and retain acceptable performance under the system faults. All present FTC method can be classified into two groups. The first group is based on fault detection and diagnostics (FDD). The second group is independent of FDD and includes methods such as integrity control, reliable stabilization and simultaneous stabilization. This paper presents the fundamental FDD-based FTC methods, which are capable of on-line detection and diagnose of the induction motors. Therefore, our group has developed the embedded distributed fault tolerant and fault diagnosis system for industrial motor. This paper presents its architecture. These mechanisms are based on two 32-bit DSPs and each TMS320F2407 DSP module is checking stator current, voltage, temperatures, vibration and speed of the motor. The DSPs share information from each sensor or DSP through DPRAM with hardware implemented semaphore. And it communicates the motor status through field bus (CAN, RS485). From the designed system, we get primitive sensors data for the case of normal condition and two abnormal conditions of 3 phase induction motor control system is implemented. This paper is the first step to drive multi-motors with serial communication which can satisfy the real time operation using CAN protocol.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.15 no.6
• /
• pp.1391-1396
• /
• 2011

A way to increase productivity in agriculture that is labor-centered industry is to graft IT technology. Today, many technologies in ubiquitous computing are deployed in all areas of society such as traffic control, automotive manufacturing, construction, defence, healthcare and clinical services. These IT technologies is gaining more attention as a fusion technology among traditional industries. To successfully build ubiquitous agriculture environment, it needs optimized core technology development for agriculture that includes sensor node H/W, middleware platform, routing protocol and agriculture environment application services. To achieve accurate botany growth environment management, we propose a green growth environment management system using environmental factor monitoring sensor and biological information sensors in greenhouse. By using our proposed system, it is expected to realize fusion complex agriculture technology with low cost.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.20 no.5
• /
• pp.972-978
• /
• 2016

MACsec is a cryptographic function that operates on Layer 2, the international standard defined in the IEEE 802.1AE. As industries such as IoT(Internet of Things) devices are receiving attention recently are connected to the network and Internet traffic is increasing rapidly, and is exposed to the risk of a variety of Internet attacks. Traditional network security technologies were often made in Layer 3, such as IPsec. However, to be increased as rapidly as the current traffic situation is complicated, and became interested in the security function of protecting the entire traffic instead of for a specific application or protocol. It appeared as these technologies is technology MACsec technology to protect all traffic in Layer 2. In this paper, we propose a Layer 2 security technology adapter MACsec MACsec a technology that allows you to simply and easily add them to the existing Layer 2 networks.

• Journal of KIISE:Computing Practices and Letters
• /
• v.10 no.1
• /
• pp.37-48
• /
• 2004

Important Java network application services have been rapidly increased along with the growth of the Internet. So, it is desirable for such applications to serve transparently, continuously and safely even if the network is temporally partitioned or certain hosts running those services are crashed down. To satisfy such requirements, many group communication systems have been developed. However, existing Java-based group communication systems do not support both the extended virtual synchrony and various types of message delivery such as FIFO, causal, total and safe delivery service. In this paper, we present the design and implementation of a Java group communication system, named JACE, supporting various types of message delivery between group members and the extended virtual synchrony model. The JACE system consists of a number of protocol modules which can be stacked on top of each other in a variety of ways. In addition, using the JACE system, we have developed an experimental UDDI registry for discovering and publishing information about Web services.

• Journal of Internet Computing and Services
• /
• v.6 no.5
• /
• pp.143-154
• /
• 2005

This paper presents a reliable multicast transmission for the advertising-supported Access Point in which a user can use a wireless network access service through receiving the advertisement. In this paper we propose a application-layer multicast protocol that controls a transmission rate of the mobile device for the reliable multicast in wireless LAN environment. Internet advertising includes all means and medias for advertising on the Internet in order to raise sales or popularity of the products or services. Since the current Internet advertising systems are passive, the target systems are exposed to unspecified persons and its exposure rates of the advertisement are changeable and unpredictable. In this paper, we propose an Internet advertising system, with which users can access the wireless Internet without charge, advertisers can provide customized advertisement according to location, time, and categories of users, and owners of network infrastructure can manage the system with a low cost.

• Journal of Animal Reproduction and Biotechnology
• /
• v.34 no.2
• /
• pp.93-99
• /
• 2019

Telomeres are known as a specialized region in the end of chromosomes to protect DNA destruction, but their lengths are shortened by repetition of cell division. This telomere shortening can be preserved or be elongated by telomerase and TERT expression. Although a certain condition in the cells may affect to the cellular and molecular characteristics, the effect of differentiation induction to telomere length and telomerase activity in mesenchymal stem cells (MSCs) has been less studied. Therefore, the present study aimed to uncover periodical alterations of telomere length, telomerase activity and TERT expression in the dental pulp-derived MSCs (DP-MSCs) under condition of differentiation inductions into adipocytes and osteoblasts on a weekly basis up to 3 weeks. Shortening of telomere was significantly (p < 0.05) identified from early-middle stages of both differentiations in comparison with undifferentiated DP-MSCs by non-radioactive chemiluminescent assay and qRT-PCR method. Telomere length in undifferentiated DP-MSCs was 10.5 kb, but the late stage of differentiated DP-MSCs which can be regarded as the adult somatic cell exhibited 8.1-8.6 kb. Furthermore, the relative-quantitative telomerase repeat amplification protocol or western blotting presented significant (p < 0.05) decrease of telomerase activity since early stages of differentiations or TERT expression from middle stages of differentiations than undifferentiated state, respectively. Based on these results, it is supposed that shortened telomere length in differentiated DP-MSCs was remained along with prolonged differentiation durations, possibly due to weakened telomerase activity and TERT expression. We expect that the present study contributes on understanding differentiation mechanism of MSCs, and provides standardizing therapeutic strategies in clinical application of MSCs in the animal biotechnology.

• Proceedings of the Korean Information Science Society Conference
• /
• 2012.06d
• /
• pp.70-72
• /
• 2012

최근 휴대용 기기들이 대중화되어 한사람이 하나 이상의 휴대용 기기를 소지하고 있고 스마트폰 활용도 또한 늘어나는 추세이다. 때문에 폭발적인 스마트폰 응용 활용이 늘어나고 있지만 현재의 애플리케이션 스토어는 각 플랫폼별로 애플리케이션을 개발해줘야 하는 종속적인 면이 있다. 앱스토어는 크게 애플의 앱스토어와 구글의 안드로이드 마켓으로 양분되어 있고 각 플랫폼에 맞춰 애플리케이션이 개발되어야 한다. 각각의 플랫폼에 맞춰서 애플리케이션을 개발하면 비용도 더 들고 다른 소규모 플랫폼(Ex 바다)들은 애플리케이션 개발자들을 끌어 모아야만 자신들의 플랫폼에 맞는 애플리케이션이 나오는 단점이 있다. 각 플랫폼에 맞춰서 개발된 네이티브 애플리케이션의 범용성에 대한 해결책과 모바일에서의 다양한 요구사항을 수용하기 위해 웹 애플리케이션이 각광받고 있다. 웹 애플리케이션은 플랫폼에 종속되지 않고 어느 휴대용 기기에서도 동작하기 때문에 각 플랫폼별로 개발하지 않아도 된다. 따라서 웹 애플리케이션 스토어끼리의 연동 프로토콜을 통하여 애플리케이션들을 어느 웹 애플리케이션 스토어에서도 볼 수 있고 플랫폼에 구애받지 않는 거대한 시장이 생겨날 수 있다. 하지만 아직 웹 애플리케이션 스토어라는 표준이 없고 존재하는 웹 애플리케이션 스토어가 없다. 이를 위해 본 논문에서는 연동에 관한 프로토콜을 제안한다.