• Journal of Korean Society of Forest Science
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• v.101 no.2
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• pp.185-194
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• 2012

The purpose of this contribution is to develop the framework of a methodology for identifying potential errors in georeferencing and in an application of it using specimens of Ox-Knee, Achyranthes bidentata Blume in Korea. At infraspecific level, uncertainty of identification showed that 41% of A. bidentata var. japonica and 28% of var. bidentata were misidentified, suggesting that the uncertainty level was independent of the reliability of experts' identification. For georeference specimen records, 71 specimens out of total 303 were selected and utilized as occurrence data: Uncertainty was 32.4 km at maximum and was 0.1297 km at minimum (mean = 4,055 m, s.d. = 5,772 m). Var. japonica is common throughout most of the southeastern Korea and west coastal areas, while var. bidentata has been found as far north as Gyeonggi and Gangwon provinces. We modelled the potential distribution of two varieties using Bioclim approach in Korea based on several environmental factors. Our results indicated the most important region for var. japonica lies the west coast ranges and southern area, while for Chungcheongnam-do of potential high diversity occurs for var. bidentata. This study shows that the major factors to determine the distribution patterns of two varieties were thermal factors, rather than precipitation. The Bioclim model using geocode and georeferencing data makes the information increasingly useful and reliable. To improve data quality, it requires full management from data collection to final databases including data cleaning.

• Molecules and Cells
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• v.40 no.9
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• pp.655-666
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• 2017

We constructed a large $na{\ddot{i}}ve$ human Fab library ($3{\times}10^{10}$ colonies) from the lymphocytes of 809 human donors, assessed available diversities of the heavy-chain variable (VH) and ${\kappa}$ light-chain variable (VK) domain repertoires, and validated the library by selecting Fabs against 10 therapeutically relevant antigens by phage display. We obtained a database of unique 7,373 VH and 41,804 VK sequences by 454 pyrosequencing, and analyzed the repertoires. The distribution of VH and VK subfamilies and germline genes in our antibody repertoires slightly differed from those in earlier published natural antibody libraries. The frequency of somatic hypermutations (SHMs) in heavy-chain complementarity determining region (HCDR)1 and HCDR2 are higher compared with the natural IgM repertoire. Analysis of position-specific SHMs in CDRs indicates that asparagine, threonine, arginine, aspartate and phenylalanine are the most frequent non-germline residues on the antibody-antigen interface and are converted mostly from the germline residues, which are highly represented in germline SHM hotspots. The amino acid composition and length-dependent changes in amino acid frequencies of HCDR3 are similar to those in previous reports, except that frequencies of aspartate and phenylalanine are a little higher in our repertoire. Taken together, the results show that this antibody library shares common features of natural antibody repertoires and also has unique features. The antibody library will be useful in the generation of human antibodies against diverse antigens, and the information about the diversity of natural antibody repertoires will be valuable in the future design of synthetic human antibody libraries with high functional diversity.

• The Journal of the Korea Contents Association
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• v.11 no.3
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• pp.26-35
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• 2011

In these days, internet environment are very quickly development as well on-line service have been using a online for the mission critical business around the world. As the amount of information to be processed by computers has recently been increased there has been cluster computing systems developed by connecting workstations server using high speed networks for high availability. but cluster computing technology are limited for a lot of IT resources. So, grid computing is an expanded technology of distributed computing technology to use low-cost and high-performance computing power in various fields. Although the purpose of Grid computing focuses on large-scale resource sharing, innovative applications, and in some case, high-performance orientation, it has been used as conventional distributed computing environment like clustered computer until now because grid middleware does not have common sharable information system. In order to use grid computing environment efficiently which consists of various grid middleware, it is necessary to have application-independent information system which can share information description and services, and expand them easily. This paper proposed new database architecture and load balancing for high availability through Grid technology.

• Journal of Industrial Convergence
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• v.18 no.5
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• pp.42-53
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• 2020

This study approached the elderly in Korea with a systematic review to find out the effect of virtual reality program arbitration on balance, which the evidence for the virtual reality program is provided. Total of 94 papers were searched through the database Nuri Media (DBpia), Scholarship (earticle), Korean Studies Information (KISS), National Digital Science Library (NDSL), the Korea Educational Research and Information Service (RISS), Kyobo Book Scholar (RISS), and Hakjisa New Thesis on Literature Selection using PRISMA flow-chart from January 2005 to May 2020 based on the final literature selection process and analysis. The quality level of the literature was found to be three volumes (50.0%) of the base level I, one (16.7%) of the II, and two of the III (33.3%). The most common type of virtual reality program was Wii-fit balance of 4 (66.7%), and the effect of virtual reality program arbitration was significant overall through evaluation tools for balance and walking ability. This is expected to effectively apply the virtual reality program to the elderly. In addition, since clinical application basis has been provided, further studies applying various virtual reality program interventions need to be addressed.

• Korean Journal of Ecology and Environment
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• v.54 no.3
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• pp.247-256
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• 2021

Understanding the selective feeding behavior of zooplankton on phytoplankton is essential for evaluating the nutrient cycle and energy flow in the food web. Although many studies have been conducted regarding the feeding behaviors of zooplankton through gut content analyses, there are limitations in the visual identification of digested contents using a microscope. DNA techniques have been applied to overcome these limitations since they can detect and amplify small amounts of prey DNA remaining in the gut contents. We designed a method to extract prey DNA from the gut contents of the whole body of the copepod specimen and tested the resolution of DNA identification for the prey phytoplankton. The common brackish species, Sinocalanus tenellus, were collected from Saemangeum Reservoir in different sites and seasons, and gut content DNA was extracted using 2.5% bleach treatment for 2 min for removal of potential contamination sources existing in preserved specimens without dissolution of the body. The sequences of the extracted gut contents were confirmed using BLASTn suite based on the NCBI database. The phytoplankton species detected in the gut showed temporal and spatial differences. Although DNA analysis of small copepod gut contents has been suggested as an effective method to examine the dynamics of primary prey sources at the genus or species level, uncertainties such as misidentification and limitations in the detailed information of the composition still exist.

• Journal of Digital Forensics
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• v.13 no.1
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• pp.35-44
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• 2019

The voice-phishing is done by inducing victims to send money, only with voice through the personal information illegally obtained. The amount of damage caused by voice-phishing continues to increase every year, and it became a social problem. Recently, the Financial Supervisory Service (i.e. the FSS) in Republic of Korea has been collecting the voices of voice-phishing scamer from victims. In this paper, we describe an effective forensic speaker analysis method for detecting the voice from the same person compared with the large-scale speech files stored in database(DB), and apply the aforementioned forensic speaker analysis method with the collected voice-phising speech files from victims. At first, an i-vector of each speech file had been extracted from the DB, then, the cosine similarity matrix for the all speech files had been generated through the cosine distance among the extracted the i-vectors of all speech file in DB. In other words, it performed the speaker analysis as grouping a set of candidates with high common similarity among i-vectors of all speech files in DB. As a result of EER(Error Equal Rate) measurement for 6,724 speech files composed of 82 speakers, it was confirmed that the EER of the i-vector-based method is improved than that of the GMM-based method. Finally, as a result of comparing the collected 2,327 voice-phishing speech files collected by the FSS, it was shown that some of the speech files having similar voice features were grouped each other.