• Korean Journal of Agricultural Science
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• v.43 no.1
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• pp.21-27
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• 2016

Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), and Apple stem pitting virus (ASPV) have been known to induce top working disease causing economical damage in apple. Occurrences of these three viruses in pome fruit trees, including apple, have been reported around the world. The transmission of the three viruses was reported by grafting, and there was no report of transmission through mechanical contact, insect vector, or seed except some herbaceous hosts of ASGV. As RNA extraction methods for fruit trees, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and multiplex RT-PCR techniques have been improved for reliability and stability, and low titer viruses that could not be detected in the past have become detectable. We studied the seed transmission ability of three apple viruses through apple seedling diagnosis using RT-PCR. Nineteen seeds obtained from commercially grown apple were germinated and two of the resulting plants were ASGV positive. Seven clones of the amplified ASGV coat protein (CP) genes of these isolates were sequenced. Overall sequence identities were 99.84% (nucleotide) and 99.76% (amino acid). Presence of a previously unreported single nucleotide and amino acid variation conserved in all of these clones suggests a possible association with seed transmission of these 'S' isolates. A phylogenetic tree constructed using ASGV CP nucleotide sequences showed that isolate S sequences were grouped with Korean, Chinese, Indian isolates from apple and Indian isolates from kiwi.

• Korean Journal of Environmental Agriculture
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• v.42 no.3
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• pp.239-252
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• 2023

Most apple trees in South Korea are grafted on M.9 and M.26 rootstocks; however, these rootstocks are susceptible to fire blight. Although M.7 rootstocks are moderately resistant to fire blight, they tend to exhibit excessive vigor, which is unsuitable for high-density planting, unless weak cultivars are used. This study investigated the vegetative growth, yield, and fruit quality of apple trees grafted onto M.7, M.9, or M.26 rootstocks to assess the feasibility of establishing high-density apple orchards domestically using the M.7 rootstock a period of seven years (1-7 years after planting). Rootstocks were tested using three cultivars with contrasting induced vigor and harvesting times: vigorous and late-maturing 'Fuji,' moderate vigor and middle-maturing 'Hongro,' and low vigor and early-maturing 'Sansa.' The planting density was maintained constant, with 190 trees per 10 a. Primary thinning (leaving only the king fruit on clusters) was performed, whereas secondary thinning (controlling crop load) was not. Vegetative growth, accumulated yield per 10 a, and yield efficiency varied depending on cultivars and rootstocks; however, the cultivars had a more notable effect on fruit quality than the rootstocks. Biennial bearing often occurred in the M.26 rootstock. 'Fuji'/M.7 was overly vigorous for high-density planting. The fruit quality and accumulated yield per 10 a of M.7 were similar to those of M.9 with the 'Hongro' and 'Sansa' cultivars. In particular, 'Hongro'/M.7 did not show tree vigor reduction due to heavy crop load, and the degree of biennial bearing in 'Sansa'/M.7 was not particularly high. These results indicated that high-density apple planting using the M.7 rootstock was achievable using the 'Hongro' and 'Sansa' cultivars.

• Korean Journal of Agricultural and Forest Meteorology
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• v.11 no.4
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• pp.127-134
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• 2009

This study was carried out to investigate the effects of air temperature during midsummer on sunburn occurrence that had some problems recently on 'Fuji'/M.9 planting system in Korea. There was a positive relationship between sunburn incidence and accumulated days with high air temperature over $31^{\circ}C$, or accumulated total quantum per day. 'Fuji'/M.9 apple trees with relatively open canopies, compared to 'Fuji'/M.26, were more susceptible to sunburn injury because the fruit was more exposed to sunlight. In 'Fuji'/M.9 apple trees, those that were poor in vigor or with short bourse shoot length were more susceptible to sunburn injury. Sunburn incidence was affected by neither regions nor row orientations. Sunburn was observed most often on the fruits on the south and west sides of the trees. The reason was due to high skin temperature of the fruits reaching $40{\sim}45^{\circ}C$ under full sunlight.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.1-10
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• 2015

This study was conducted in three years (7-9 years after planting) to investigate vegetative growth, yield, fruit quality, and return bloom for optimum crop load based on different planting densities of adult 'Fuji'/M.9 apple trees. As plant materials, 'Fuji'/M.9 apple trees planted at $3.5{\times}1.5m$ (190 trees per 10 a), $3.5{\times}1.2m$ (238 trees per 10 a), and $3.2{\times}1.2m$ (260 trees per 10 a) spacing and trained as slender spindles were used. The crop load was assigned to five different object ranges as follows: 55-64, 65-74, 75-84, 85-94, and 95-104 fruit per tree. TCA increment, total shoot growth, return bloom, yield per tree, and yield efficiency tended to increase as planting density decreased, and fruit weight and soluble solid content tended to increase as the object range of crop load decreased. Fruit red color tended to increase as shoot growth decreased. For apple trees planted with 238 trees and 260 trees per 10a, biennial bearing occurred when the crop load was over 85-94 and 75-84 fruits, respectively. However, biennial bearing did not occur when the crop load was 95-104 fruits in apple trees planted with 190 trees per 10a. Accumulated yield tended to increase as planting density and crop load increased, but that of biennial bearing did not show such a difference. Based on our results, optimum crop load recommendations are to set 95-104 fruits per tree in 'Fuji'/M.9 mature apple trees planted at 190 trees per 10a, 75-84 fruits per tree at 238 trees per 10a, and 65-74 fruits per tree at 260 trees per 10a.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.155-165
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• 2015

Well-feathered (over 10 feathers) 'Fuji'/M.9 apple trees were planted at $3.0{\times}1.0m$ and trained to slender spindle with 2.5 m height or to tall spindle with 3.5 m height, and the vegetative growth, productivity, and fruit quality of two training systems were compared for 8 years. The canopy volume of the tall spindle trees surpassed that of the slender spindle trees 4 years after planting and was 25% larger than that of the slender spindle trees 5 years after planting. The accumulated yield over 8 years for the tall spindle system was 14% higher than that of the slender spindle system. Alternate bearing and incidence of marssonina blotch were observed in both treatments after 5 years of planting. There was often vegetative imbalance in the trees however, the degree of yield loss and vegetative imbalance of the tall spindle trees was lower than those of the slender spindle trees. Soluble solid content and fruit red color of the tall spindle trees were higher than that of the slender spindle trees in 5 year after planting, resulting from increased light penetration in the canopy due to even distribution of lateral branches and from fruit bearing in different height locations of the trees. In conclusion, increasing the tree height to about 3.5 m using slender spindle 'Fuji'/M.9 apple trees planted with over 333 trees per 10a led to better light penetration, yield and fruit quality compared to a conventional wide training system with the slender spindle.

• The Plant Pathology Journal
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• v.30 no.2
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• pp.195-199
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• 2014

Apple stem pitting virus (ASPV), of the Foveavirus genus in the family Betaflexiviridae, is one of the most common viruses of apple and pear trees. To examine variability of the coat protein (CP) gene from ASPV, eight isolates originating from 251 apple trees, which were collected from 22 apple orchards located in intensive apple growing areas of the North Gyeongsang and North Jeolla Provinces in Korea, were sequenced and compared. The nucleotide sequence identity of the CP gene of eight ASPV isolates ranged from 77.0 to 97.0%, while the amino acid sequence identity ranged from 87.7 to 98.5%. The N-terminal region of the viral CP gene was highly variable, whereas the C-terminal region was conserved. Genetic algorithm recombination detection (GARD) and single breakpoint recombination (SBP) analyses identified base substitutions between eight ASPV isolates at positions 54 and 57 and position 771, respectively. GABranch analysis was used to determine whether the eight isolates evolved due to positive selection. All values in the GABranch analysis showed a ratio of substitution rates at non-synonymous and synonymous sites (dNS/dS) below 1, suggestive of strong negative selection forces during ASPV CP history. Although negative selection dominated CP evolution in the eight ASPV isolates, SLAC and FEL tests identified four possible positive selection sites at codons 10, 22, 102, and 158. This is the first study of the ASPV genome in Korea.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.352-357
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• 2001

This experiment was carried out in order to develop some chip budding techniques related to the production of high quality maiden apple trees. Optimum chip budding time for apple trees appeared to be from mid-August to early September. Untying wrapping tapes after 6 8 weeks from budding led to higher graft success and better shoot growth than removing the tapes earlier or later than the 6-8 weeks. Dipping pre-cut bud chips into ordinary water of $25^{\circ}C$ for 5 hours did not hamper the graft-success nor the early season growth of the graft compared with non-treated control. Dipping the pre-cut bud-chips into the $35^{\circ}C$ water for 3 hours or for 5 hours hampered the union formation and shoot growth of the 'Fuji' apple trees.

• Journal of Biosystems Engineering
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• v.19 no.4
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• pp.311-317
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• 1994

Warts and barks of apple trees were vector of White rot. Two devices removing warts and barks for the use of pest control on apple trees were developed and evaluated in this study, and their results are as follows ; 1. A total of 148 warts were examined to determine the average size. About 35 percent of the examined warts were 6~8mm long, 4~8mm wide and 4~6mm thick in size. About 30 percent of the examined warts were 4~6mm long, 2~4mm wide and 6~8mm thick in size. 2. Seventy-one percent of the examined barks removed were 4~8mm in thickness. 3. A blade with 30 degree of cutting angle required 3 to 22 percent less cutting energy than those with 15 and 45 degrees of cutting angles. 4. The cutting torque decreased from 31 N-cm to 12 N-cm with an increase of cutting speed from 26cm/s to 104cm/s for a feeding speed of 0.31 mm/s with the blade angle of 30 degrees. 5. The cutting torque increased from 6N-cm to 32N-cm with an increase of branch diameter from 6mm to 14mm for a feeding speed of 0.31 mm/s with the blade angle of 30 degrees. 6. Two devices mounted on a mower for removing warts and barks were evaluated and proved effective.

• The Korean Journal of Mycology
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• v.42 no.3
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• pp.183-190
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• 2014

Apple blotch is a major apple disease and recently it causes critical economic losses in apple orchards in Korea. In this review, we described the history of apple blotch researches, ecology and life cycle of Marssonina coronaria, cultural and molecular characteristics and simple isolation method as well. Furthermore, characteristic of apple blotch like symptoms, its cause still unknown, occurs frequently in cv. "Fuji" was described in detail.

• The Korean Journal of Mycology
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• v.49 no.3
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• pp.417-423
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• 2021

Apple decline symptoms were frequently observed on cv. Fuji apple orchards located in Gyeonggi, Gyeongbuk, and Gangwon provinces during surveys conducted from May until the end of September 2020. Three fungal strains were isolated from the margins of internal lesions of diseased apple trees, and their morphological characteristics were considered similar to Botryosphaeria sinensis. Phylogenetic analysis using internal transcribed spacer (ITS) regions, translation elongation factor 1-alpha (tef1), beta-tubulin (tub2), and the second largest subunit of RNA polymerase II (rpb2) gene sequences confirmed the closest relationship of isolates with B. sinensis at the species level. According to a pathogenicity test, the appearance of dark-brown discolorations and vascular necrosis on apple branches inoculated with the isolated strain KNUF-20-014 was observed. To the best of our knowledge, this is the first report of B. sinensis as the causal agent of apple disease in Korea.