The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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First Report of Apple Decline Caused by Botryosphaeria sinensis in Korea

  • Lee, Seung-Yeol (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Ten, Leonid N. (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Back, Chang-Gi (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Jung, Hee-Young (College of Agriculture and Life Sciences, Kyungpook National University)
  • Received : 2021.09.03
  • Accepted : 2021.09.27
  • Published : 2021.09.30
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Abstract

Apple decline symptoms were frequently observed on cv. Fuji apple orchards located in Gyeonggi, Gyeongbuk, and Gangwon provinces during surveys conducted from May until the end of September 2020. Three fungal strains were isolated from the margins of internal lesions of diseased apple trees, and their morphological characteristics were considered similar to Botryosphaeria sinensis. Phylogenetic analysis using internal transcribed spacer (ITS) regions, translation elongation factor 1-alpha (tef1), beta-tubulin (tub2), and the second largest subunit of RNA polymerase II (rpb2) gene sequences confirmed the closest relationship of isolates with B. sinensis at the species level. According to a pathogenicity test, the appearance of dark-brown discolorations and vascular necrosis on apple branches inoculated with the isolated strain KNUF-20-014 was observed. To the best of our knowledge, this is the first report of B. sinensis as the causal agent of apple disease in Korea.

Keywords

Acknowledgement

This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ014998022021), funded by the Rural Development Administration, Republic of Korea.

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