Two experiments were carried out to study the effects of corticosterone (CORT) administration on intestinal morphology and function of broilers. In both experiments, birds were randomly divided into two equal groups. One group was the control group (CTRL), and the birds were fed with a basal diet. The other was the experimental group (CORT), and the birds were fed with the basal diet plus 30 mg of CORT/kg diet. At 21 days of age, performance, morphological characteristics of intestine, D-xylose level in plasma, activities of digestive enzymes in digesta, digestibility of nutrients and 5-bromo-2-deoxyuridine (BrdUrd)-labeling index of intestinal epithelial cells were determined. CORT administration decreased feed intake, daily gain and feed conversion ratio (p<0.05). CORT also decreased duodenal and jejunal villus height (p<0.05) as well as crypt depth (p<0.05). The D-xylose level in plasma of CORT-treated broilers was lower than that of the control (p<0.05). CORT treatment caused a decrease in apparent digestibility of protein (p<0.05), whereas fat and starch apparent digestibilities were unaffected (p>0.05). CORT administration increased activities of trypsin and amylase (p<0.05), and decreased BrdUrd-labeling index of duodenal and jejunal epithelial cells (p<0.05). In conclusion, CORT administration impaired the normal morphology and absorptive capacity of the small intestine of broiler chickens.
The effect of cooking methods on in vivo and in vivo indices of the protein quality of hagfish meat were investigated. In vivo protein digestibilities of cooked meats (81.3~83.5 %) were not significant different (p<0.05) from those of van meat (82.9%), with the exception of steamed (11$0^{\circ}C$, 15 min) meat (86.3 %). Convection oven cooking (22$0^{\circ}C$, IS min) resulted in a higher trypsin indigestible substrate (TIS, 49.2 mg/g solid) compared with that of raw meat (38.9 mg/g solid). free amino acid content of raw meat was decreased after boiling (10$0^{\circ}C$, 10min). Both convection oven and microwave cooking (2,450 MHz, 3 min) decreased available lysine from 4.9g/16g N to 3.8~4.1g/16g N. In vivo apparent protein digestibilites (AD) of hagfish meat were similar fur raw (92.4%) and cooked meats, but were somewhat lower than ANRC (Animal Nutrition Research Council) casein (945%). The PERs (3.7~4.1) and NPRs (3.7~4.9) of cooked meats were significantly higher (p<0.05) than those of raw meat (PER 3.3, NPR 3.6 and ANRC casein (PER 2.5, NPR 2.6), despite their lower in vivo protein digestibilities. These results demonstrate that cooking at optimal conditions resulted in remarkably positive effects on in vivo and in vivo protein qualities of hagfish meats. Therefore, steamed hagfish meat is an excellent source of high quality protein from seafood products.
Kim, Young-Il;Bae, Ji-Sun;Jee, Kyung-Su;McCaskey, Tom;Kwak, Wan-Sup
Asian-Australasian Journal of Animal Sciences
/
제24권12호
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pp.1744-1751
/
2011
This study was conducted to compare the effects of feeding dry or fermented (aerobically or anaerobically with or without lactic acid bacteria) restaurant food residue mixture-containing diets on animal performance and blood profiles. Rats were used as the model animal for the simulation of laboratory rodents, rabbit or horse feeding and fed for 4 wks. The results were compared with feeding a dry diet (control) with the same ingredient composition as diets processed by aerobic and anaerobic methods. Feeding all the fermented diets tended to increase (p>0.05) average daily gain of rats resulting in improved (p<0.01) feed efficiency. Apparent digestibility of NDF was increased (p<0.05) by feeding the fermented diets, although digestibilities of DM, OM, CP, and NFC were not affected (p>0.05). Compared with the aerobically fermented diet, digestibility of ADF was increased (p<0.05) for the anaerobically fermented diet and for the 0.5% LAB culture plus anaerobically fermented diet. The digestibility of crude ash tended to increase (p>0.05) with feeding of the fermented diets. Feeding either of the fermented diets had little effects on serum nutrients, electrolytes, enzymes and blood cell profiles of rats except sodium and uric acid concentrations. These results showed that compared with feeding a dry food residue-containing diet, feeding aerobically or anaerobically fermented diets showed better animal performance as indicated by higher feed efficiency and rat growth rate. These improvements were attributed to the desirable dietary protein conservation during the food residue fermentation process and to higher total tract digestibilities of NDF and crude ash in the fermented food residue diets.
Khan, M. Ajmal;Sarwar, Muhammad;Nisa, M.;Khan, M.S.;Bhatti, S.A.;Iqbal, Z.;Lee, W.S.;Lee, H.J.;Kim, H.S.;Ki, K.S.
Asian-Australasian Journal of Animal Sciences
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제19권5호
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pp.645-650
/
2006
Thirty early lactating Nili-Ravi buffaloes, six animals in each group, were used in a completely randomized design to examine the feeding value of 4% urea treated wheat straw (UTWS) ensiled with 6% or without acidified molasses. Five experimental diets were formulated. The control ration was balanced to contain 30% DM from UTWS ensiled without acidified molasses. The other four diets were formulated to have 30, 40, 50 and 60% DM from UTWS ensiled with 6% acidified molasses, respectively. Dry matter and neutral detergent fiber (NDF) intakes were higher in buffaloes fed diets containing UTWS ensiled with acidified molasses compared with those fed a diet containing UTWS ensiled without acidified molasses. Intake of DM was not significantly different in buffaloes fed diets containing varying levels of UTWS ensiled with acidified molasses. A similar trend was observed for crude protein (CP) intake. Apparent DM and NDF digestibilities were significantly higher in buffaloes fed diets containing UTWS ensiled with acidified molasses compared with those fed UTWS ensiled without acidified molasses. However, differences in DM and NDF digestibilities were non-significant across buffaloes fed diets containing varying levels of UTWS ensiled with acidified molasses. Milk yield (4% fat corrected) was significantly higher in buffaloes fed diets containing UTWS ensiled with acidified molasses than those fed a diet containing UTWS ensiled without acidified molasses. Milk yield was similar in buffaloes fed varying level of UTWS ensiled with acidified molasses. Milk CP, true protein, solid-not-fat and total solids were similar in buffaloes fed UTWS ensiled with or without acidified molasses. The UTWS ensiled with 6% acidified molasses can be included at up to 60% DM of lactating buffalo rations without any ill effect on productivity.
In a split-unit design, 12 Javan rusa (Cervus timorensis russa) stags (6 castrated and 6 entire) were used to investigate seasonal (winter, spring and summer) effects on intake, digestibility of food constituents, volatile fatty acid profile, and nitrogen retention, when given lucerne (Medicago sativa) or rhodes grass (Chloris guyana) hays. Entire stags ate 9% more dry matter (DM) than castrates (p<0.05). Both castrated and entire stags exhibited seasonal changes in DM intake, these being higher (p<0.05) in winter (62.3 g/kg $W^{0.75}$) than spring (56.9 g/kg $W^{0.75}$) or summer (55.3 g/kg $W^{0.75}$). Intakes of lucerne hay (211 g protein/kg DM) were significantly higher than of rhodes grass hay (49 g protein/kg DM) in all seasons (p<0.05). Digestibilities of DM (0.58), neutral detergent fibre (0.59) and acid detergent fibre (0.47) were similar between castrates and entires. DM digestibility was higher (p<0.0001) for lucerne (0.66) than rhodes grass (0.55), and was higher (p<0.05) in winter (0.60) than spring (0.58) or summer (0.57). The ruminal acetate concentrations were higher (p<0.001) in spring than summer (78 and 73 molar % respectively). Apparent digestibility of protein was significantly less (p<0.0001) for rhodes grass (0.37) than lucerne (0.75). N retention was positive for lucerne (15.2 g/d) but negative for rhodes grass (-2.8 g/d) (p<0.0001), and was higher (p<0.001) in summer (12.0 g/d) than spring (4.3 g/d) or winter (2.4 g/d). The tropical rusa deer exhibits seasonal variations in feed intake, food constituent digestibilities, VFA profile and N retention. Castration did not alter these traits. The results do not support the view that rusa deer can thrive on low-quality pastures. The productivity and commercial exploitation of rusa deer could be optimised if they are given high-protein feed during spring and summer.
Piao, X.S.;Li, Defa;Han, In K.;Chen, Y.;Lee, J.H.;Wang, D.Y.;Li, J.B.;Zhang, D.F.
Asian-Australasian Journal of Animal Sciences
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제15권1호
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pp.89-93
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2002
A total of six crossbred barrows ($Duroc{\times}Landrace{\times}Large$ White, $44.17{\pm}1.94kg$ BW) were housed conducted to evaluate apparent fecal digestibilities of Brown Rice (BR) as an alternative energy source in growing pigs. Pigs were housed individually on metabolism crate on the basis of body weight. Four treatments contained: 1) 100% of corn-soybean meal (C100; Control diet), 2) 75% of corn-soybean meal diet plus 25% of corn meal (C25), 3) 100% of brown rice-soybean meal diet (BR100), 4) 75% of brown rice-soybean meal diet plus 25% of brown rice meal (BR25). Brown rice has an excellent gross energy and crude protein composition compared to corn. The BR used had 3,801 kcal of gross energy/kg, 8.0% crude protein, 2.6% of ether extract, 0.035% calcium and 0.35% total phosphorus. The best digestibilities of energy (87.75%), DM (81.71%) and CP (78.57%) were observed in BR 100 group and the worst were found in Corn 25 group. The nutrient digestibility was not significantly different in most nutrients. Through this experiment, BR appeared a good alternative energy source that can replace corn yellow to 100% in growing pigs. Therefore, the price relationship between corn and BR may provide an excellent opportunity for pork producers to use BR in order to reduce feed costs provided that diet has been balanced for digestible amino acids.
Ninety nursery pigs were used in two metabolism experiments to determine the effects of roasting and extruding on the nutritional value of Williams 82 soybeans with (+K) and without (-K) gene expression for the Kunitz trypsin inhibitor. Treatments for both experiments were: 1) soybean meal; 2) +K roasted; 3) +K extruded; 4) -K roasted; and 5) -K extruded. Diets were the soybean preparations (96.5% of the diet) with only vitamins and minerals added as needed to meet or exceed NRC recommendations. Daily feed allowance was 5% of initial BW given as three equal meals. In Exp. 1, 50 weanling pigs (4.7 kg average BW and 21 d average age) were used. Apparent values for N digestibility (p<0.001), biological value (p<0.09), percentage N retention (p<0.05), GE digestibility (p<0.001) and percentage ME (p<0.001) were greater for pigs fed extruded soybeans than pigs fed roasted soybeans. Also, N digestibility (p<0.05), biological value (p<0.03) and percentage N retention (p<0.04) were greater for pigs fed -K soybeans than those fed +K soybeans. In Exp. 2, 40 pigs (9.7 kg average BW and 35 d average age) were allowed to adjust to the nursery environment before use in the experiment. In general, the pigs in Exp. 2 (i.e., the older pigs) had greater utilization of nutrients from all of the soybean products than the younger pigs used in Exp. 1. Digestibilities of DM, N and GE were greater (p<0.001) for pigs fed -K soybeans than those fed +K soybeans and extruded soybeans had greater digestibilities of DM, N and GE than roasted soybeans (p<0.001). Also, percentage N retention (p<0.01) and percentage ME (p<0.001) for pigs fed extruded soybeans were greater than for pigs fed roasted soybeans. In conclusion, extruded and -K soybeans were greater nutritional value than roasted and +K soybeans for 4.7 and 9.7 kg nursery pigs.
Li, Defa;Xu, X.X.;Qiao, S.Y.;Zheng, C.T.;Chen, Y.;Piao, X.S.;Han, In K.;Thacker, P.
Asian-Australasian Journal of Animal Sciences
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제13권3호
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pp.369-375
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2000
Two experiments were conducted to determine the apparent ileal digestibility of the amino acids contained in peanut meal using the regression technique and then applying the values obtained, in a growth trial, using growing-finishing pigs. For the digestibility trial, four 20 kg crossbred (Yorkshire${\times}$Landrace${\times}$Beijing Black) barrows were fitted with simple T-cannula in the terminal ileum. After recovery, the barrows were fed one of four experimental diets according to a $4{\times}4$ Latin Square design. The pigs were fed corn-soybean meal based diets supplemented with 0, 25, 50 or 75% peanut meal. For the growth trial, 200 crossbred (Yorkshire${\times}$Landrace${\times}$Beijing Black) growing pigs (20.9 kg) were fed corn-soybean meal diets supplemented with 0, 5, 10, 15 or 20% peanut meal. Five pens (4 gilts and 4 castrates) were assigned to each treatment. With the exception of arginine, histidine and phenylalanine, the digestibility coefficients for the indispensible amino acids declined as the level of peanut meal in the diet increased. There was a good agreement between the amino acid digestibilities for lysine, methionine, threonine and tryptophan determined using the regression technique and amino acid digestibilities previously published for peanut meal. During both the growing (21-54 kg) and finishing (54-99 kg) periods, the addition of peanut meal decreased average daily gain (p=0.01) and feed conversion in a linear manner (p<0.05). Feed intake was not significantly different among treatments. The overall results suggest that peanut meal can be used at levels up to 15% in diets fed to growing-finishing pigs provided that the diet has been balanced for digestible amino acids.
Insufficient pre-slaughter fasting leaves serious amount of feed-like contents (designated here as un-fasted stomach spent feed, USSF) in the eviscerated pig stomach. This study was intended to evoke economical and environmental seriousness of USSF discharge by estimating its value as pig feed. For finishing pigs feeding trial, three levels (0, 5, and 10%) of USSF were blended with pig feed to prepare control and two treatment diets, respectively. A total of 42 (21 males, 21 females) crossbred (Landrace ${\times}$ Yorkshire ${\times}$ Duroc) finishing pigs weighing $81.5{\pm}8.0$ kg were employed to 28d feeding trial and in vivo digestibility trial by $Cr_2O_3$ indicator method with 7 males and 7 female pigs per treatment. In vitro total tract digestion of USSF showed 70.5% and 57.6% of DM and OM digestibilities, respectively which were poorer (p<0.05) than those of pig diet. There were no differences in body weight gain, daily feed intake and feed conversion ratio among treatments although 10% USSF substitution exerted relatively poor performance. In vivo apparent digestibilities of diets containing USSF 5% and 10% were lower (p<0.05) than that of 100% pig feed. There were no differences (p>0.05) in dressing percentage and carcass grade among treatments. Results of this study showed that 5% USSF substitution in finishing pigs diet did not exert any disadvantage in terms of production performance and carcass grade. This study implied that un-fasted slaughter causing excessive excretion of USSF should be avoided. If not avoidable, the USSF should not be wasted in abattoir but could be recycled as pig feed.
Objective: This study aimed at studying the potential use of Flemingia (Flemingia macrophylla) as a protein source fodder to improve nutrients digestibility and ruminal fermentation efficiency in beef cattle. Methods: Four, Thai native beef cattle were randomly assigned in a 4×4 Latin square design. Four levels of Flemingia hay meal (FHM) were used to replace soybean meal (SBM) in the concentrate mixtures in four dietary treatments replacing levels at 0%, 30%, 60%, and 100% of SBM. Results: The experimental findings revealed that replacements did not effect on intake of rice straw, concentrate and total dry matter (DM) intake (p>0.05). However, the apparent digestibilities of DM, organic matter, crude protein, acid detergent fiber, and neutral detergent fiber were linearly increased up to 100% replacement levels. Moreover, the production of total volatile fatty acids, and propionate concentration were enhanced (p<0.05) whereas the concentration of acetate was reduced in all replacement groups. Consequently, the CH4 production was significantly lower when increasing levels of FHM for SBM (p<0.05). Furthermore, rumen bacterial population was additionally increased (p<0.05) while protozoal population was clearly decreased (p<0.05) in all replacement groups up to 100%. In addition, microbial nitrogen supply and efficiency of microbial nitrogen synthesis were enhanced (p<0.05), as affected by FHM replacements. Conclusion: The findings under this experiment suggest that 100% FHM replacement in concentrate mixture enhanced rumen fermentation efficiency, nutrients digestibilities, bacterial population, microbial protein synthesis, and subsequently reduced CH4 production in beef cattle fed on rice straw.
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