• 환경생물
• /
• 제33권4호
• /
• pp.433-440
• /
• 2015

The Apostichopus japonicus is an important species in some Asia countries including Korea, China and Japan. The purpose of the present study was to investigate the differential gene expression of heat shock protein90 (Hsp90) and ferritin as a biomarker for the thermal stress during water temperature rising in the sea cucumber, A. japonicus. The A. japonicus (1.4 g) was cultured in incubator of separate temperature ($15^{\circ}C$, $20^{\circ}C$, $25^{\circ}C$ and $30^{\circ}C$) for each 0, 3, 6, 12, 24, 48 hours. The mRNA expression levels of Hsp90 and ferritin were examined using RT-PCR assay. Results showed that, the expression of Hsp90 mRNA was not significantly changed at $15^{\circ}C$. The expression of Hsp90 mRNA was significantly increased at high temperature such as $20^{\circ}C$ and $25^{\circ}C$. Furthermore, Hsp90 mRNA was early increased at $25^{\circ}C$ than $20^{\circ}C$. The ferritin mRNA was similar expression pattern with Hsp90. But, Hsp90 mRNA was more sensitive than ferritin mRNA at high thermal stress. These results indicate that Hsp90 and ferritin mRNAs were involved in the temperature changes response and may be play an important role in mediating the thermal stress in A. japonicas.

• 한국수산과학회지
• /
• 제49권3호
• /
• pp.330-336
• /
• 2016

Pollution caused by fecal solids released from recirculating aquaculture systems (RAS) is a growing global concern requiring immediate attention. Thus, this study investigated the physicochemical characteristics of fecal solid feed from RASs used for eel and rainbow trout farming. The concentrations of proteins, lipids, crude ash, and moisture in eel fecal solids were 10.9%, 1.2%, 85.8%, and 72.2%, respectively, while those in rainbow trout fecal solid feed were 20.5%, 3.2%, 70.9%, and 87.0%, respectively. The control group was fed a mixture of sea cucumber and dried sea mud. The protein digestibilities of eel and rainbow trout fecal solids were 25.43% and 23.96%, respectively, while the respective lipid digestibilities were 35.62% and 36.80%, respectively.

• 한국수산과학회지
• /
• 제49권3호
• /
• pp.337-342
• /
• 2016

Sea cucumbers have been an important part of human diets throughout history; demand is increasing every year, making the species a target for coastal resource development. New harvesting technologies have been developed in response to demand, including nursery production and insemination aquaculture, but such methods produce relatively few individuals and productivity per unit area is very small. For example, captures are prohibited during July and August due to the breeding season, and sea cucumbers aestivate during the hot season and hibernate during the cold season, all factors that limit natural-environment production in Korea. Problems with slow growth of sea cucumbers have been encountered in previous rearing experiments. To assess the importance of feeding ecology on sea cucumber growth, we conducted pond-bottom and lantern-net culture experiments. Results showed that sea cucumbers in bottom cultures grew from 3.06 to 102.59 g, but those in lantern cultures only grew from 25.60 to 28.03 g.

• 미생물학회지
• /
• 제49권3호
• /
• pp.237-244
• /
• 2013

16S rRNA 유전자를 대상으로 454 파이로서열 분석법을 이용하여 해삼(Apostichopus japonicus)과 새우(Litopenaeus vannamei)의 장내 세균의 다양성을 분석하였다. 해삼의 경우, 대부분의 서열은 두 개의 속과 연관성이 있는 것으로 나타났다. 하나는 Fusobacteria 문의 Propionigenium 속이며, 다른 하나는 Bacteroidetes 문의 Flavobacteriaceae 과에 속하는 미분류 속이었다. 새우는 해삼에 비해 다양한 속들을 포함하고 있었으며 Lactococcus, Leuconostoc, Prochlorococcus, Vibrio 속들과 Flavobacteriaceae, Rhodobacteraceae, Desulfobulbaceae, Helicobacteraceae 과와 Mycoplasmatales 목에 속하는 미분류 속들을 포함하고 있었다. 해삼과 새우의 속들의 절반 이상이 환경에서 비배양적으로 얻어진 서열만 존재하는 미분류된 속인 것으로 확인되었다. 대용량 454 파이로서열 분석법을 통하여 해삼과 새우의 장내 세균의 다양성을 밝힐 수 있었으며, 그 결과 해삼과 새우는 아직까지 밝혀지지 않는 새로운 미생물을 많이 포함하고 있는 것을 알 수 있었다.

• 한국수산과학회지
• /
• 제51권2호
• /
• pp.142-147
• /
• 2018

This study was conducted to investigate the effects of practical diets containing different seaweed powders on the growth and body composition of juvenile sea cucumber Apostichopus japonicus. Six experimental diets were formulated to contain 40% of the following seaweed powders: Sargassum thunbergii (ST), Undaria pinnatifida (UP), Ascophyllum nodosum (AN), Hizikia fusiformis (HF), fermented Laminaria japonica (FLJ), and fermented Hizikia fusiformis (FHF). Sea cucumbers (30 per tank; initial mean weight $0.4{\pm}0.01g$) in three replicates of six groups were fed one of the six experimental diets for 15 weeks. Survival in all groups was 83-93%, with no significant differences among the experimental groups. The specific growth rates of the sea cucumbers fed the ST and UP diets were significantly higher than those of sea cucumbers fed the HF, FLJ, and FHF diets, but did not significantly differ from those of sea cucumbers fed the AN diet. The results of this study suggest that S. thunbergii, U. pinnatifida, and A. nodosum could be useful sources of seaweed powder for practical diets of juvenile sea cucumber.

• 한국수산과학회지
• /
• 제52권3호
• /
• pp.232-240
• /
• 2019

Approximately 80% of the world's sea cucumbers Apostichopus japonicas are processed into dried sea cucumbers. The hot air-drying method is currently used in industry, but it has many problems, such as a short drying time, severe browning, high nutrition loss, and low recovery. In this study, the moisture absorption rate, dry recovery rate, and lipid nutrient composition of sea cucumber dried by heat pump decompression and with a hybrid dryer were investigated. The moisture absorption rates for hybrid-dried sea cucumbers at 24, 48, 72, 96, and 120 h were 241.3%, 427.7%, 652.0%, 721.0%, and 742.2%, respectively. The moisture absorption rates for hot air-dried sea cucumbers were 155.8%, 240.0%, 390.3%, 655.5%, and 667.4%, respectively. Thus, moisture absorption was faster and greater with hybrid drying than with hot air drying. The dry recovery rate at 24 h was greater for hybrid-dried sea cucumber (70.7%) than for hot air-dried sea cucumber (59.8%). Saturated fatty acid contents of the hybrid- and hot air-dried sea cucumbers were 30.0% and 37.5%, respectively. Moreover, greater ${\Sigma}n-3$ polyunsaturated fatty acid content was found in hybrid-dried sea cucumber (15.8%) than in hot air-dried sea cucumber (11.7%).

• Fisheries and Aquatic Sciences
• /
• 제25권5호
• /
• pp.264-275
• /
• 2022

A non-destructive environmental DNA protocol for the genetic analysis of sea cucumber (Apostichopus japonicus) resources DNA was established. Among the several commercial DNA extraction kits, the DNeasy^® Plant Mini Kit was selected as the best choice to obtain the high-quality genomic DNAs from the mucous sea cucumber. As the temperature and incubation time increased, the amount of extracted environmental DNA was also large, but it was judged that the increased amount did not affect as much as 2-3 times. Therefore, these conditions were not considered to be the main factors to consider in actual environmental DNA extraction. However, the amount of seawater relative to the size of the sample was judged as a major consideration, and a sufficient amount of environmental DNA for analysis was secured when stored within 1 min while stirring the volume of seawater corresponding to the total sea cucumber weight (g). In securing the environmental DNA of sea cucumbers, the mortality rate of sea cucumbers in all experiments was 0, and it was judged that the effects of sea cucumbers were not significant through this treatment. Through the results of this study, sea cucumber DNA research, which has been conducted in a destructive method, can be conducted non-destructively through environmental DNA analysis. Through this study, we have secured a standard protocol that can successfully extract the sea cucumber DNA through environmental DNA. It is not only excellent in terms of time and cost of traditional DNA analysis method currently used, but it is completely non-destructive in the ecosystem of the survey area. It is believed that the system can be transformed in a way that does not affect it. However, it is thought that various standard protocols should be established considering the characteristics of each type.

• 생명과학회지
• /
• 제28권6호
• /
• pp.681-687
• /
• 2018

홍해삼 또는 Apostichopus japonicas는 동남아시아에서 발견되는 stichopodiae의 한 종이다. 본 연구에서는 홍해삼의 화장품 소재로서 사용가능한지 알아보기 위해 홍해삼 추출물의 미백, 항주름에 관한 실험을 진행하였다. tyrosinase 활성 분석 결과, 홍해삼 추출물 $200{\mu}g/ml$에서 tyrosinase 활성을 억제하였다. 또한, 홍해삼은 tyrosinase, TRP-1, TRP-2, MITF 및 matrix metalloproteinase (MMPs)의 mRNA 발현을 억제하였다. 이어서 HaCaT과 human Fibroblast를 이용한 3차원 세포배양을 통해 홍해삼의 피부 턴오버 주기 개선효과를 검증하였다. 이러한 결과를 바탕으로 홍해삼이 높은 미백효과 및 주름개선효과를 가지는 화장품 소재로서 충분한 가치를 지닐 것으로 판단된다.