• International Journal of Advanced Culture Technology
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• v.11 no.1
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• pp.356-362
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• 2023

Identifying the distribution of species and specific factors related to it is very important because it is habitat conservation that is greatly linked not only to ecological research but also to human health. After all, protecting each one's habitat will protect wildlife and further maintain a healthy ecosystem. Therefore, in this study, data were collected, compared, and analyzed through existing studies and field-oriented surveys. The results of the survey confirmed high species diversity in agricultural areas and forest areas that can reduce moisture supply and moisture loss, and the non-segmental environment, that is, ecological connectivity, was largely influenced by amphibian species diversity. It was confirmed that the non-fragmented environment, that is, ecological connectivity, was greatly affected by amphibian species diversity. In addition, the three factors that affect amphibian survival are: It was identified as the effect of not establishing a buffer space, the effect on the ecological transformation around the habitat during the summer rainy season, and the effect on the disconnected ecological environment.

• Korean Journal of Environmental Biology
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• v.26 no.1
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• pp.22-29
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• 2008

Bombina orientalis, or Korean fire-bellied toad is belonging to Class Amphinia, Order Anura, Family Bombinatoridae, Genus Bombina. Ecological characteristics, such as small size, slow moving and swimming, gentle attitude to other species, under laboratory and/or wild condition lead the toad as suitable experimental animals with easy handling. Seasonal reproductive biology except breeding season (usually from Apr. to late Jun.) are unknown still caused by sampling methods for hibernating specimens have not been established yet. Most species of amphibian, include B. orientalis, are declined around the world related with global warming, therefore, wild caught specimen for any purposes must be limited controled soon. Even though, some pre-studies of biotope for increasing biodiversity have been reported, construction of an artificial biotope to get natural breeding cycling specimen for study of amphibian reproduction is unique modelling and only trial. In an effort to set year-round sampling for study of reproduction of B. orientalis, we suggest protocols for planning, construction manual and SOP(Standard Operation Process) to manage amphibian biotope. During pilot operation of a designed and constructed biotope, the seasonal acclimation during winter hibernation of B. orientalis were successful. It will be helpful for studying the reproductive biology of wild amphibians in temperate region and allow to get reasonable research conditions in amphibian laboratory.

• Parasites, Hosts and Diseases
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• v.51 no.4
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• pp.427-432
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• 2013

A new trichostome ciliate, Balantidium honghuensis n. sp., was isolated from the recta of Rana nigromaculata and R. limnocharis during parasite surveys in Honghu Lake, Hubei Province, central China in summmer of 2010 and 2011. Its detailed morphometric characters based on LM and SEM studies were described herein. The organism is oval in shape and thickly ciliated. The vestibulum is "V" shaped and occupies about 1/3 to 2/5 of the body length. The vestibular and nearby regions possess strong peripheral fibers which form a marked axial fiber about the cytopharynx. More than 10 contractile vacuoles are distributed along the periphery of the latter body. Comparisions were made between this new species and B. sinensis Nie, 1935. They were discriminated from each other in terms of general body forms, body size, and vestibulum shapes. Besides, special attention was paid to its high-speed daughter swarmers which we believed to be the infective stage of B. honghuensis. Possible infection routes of anura amphibian balantidia were discussed.

• Animal Systematics, Evolution and Diversity
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• v.12 no.3
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• pp.253-264
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• 1996

The ultrastructure of spermatozoa in urodeles and primitive anurans was examined and compared. The spermatozoa of urodeles are characterized by seven plesiomorphies in subacrosomal cone, endounclear canal. perforatorium, ring, marginal filament, undulating membrane and tail axis. Most primitive anuran spermatozoa have no marginal filament, subacrosomal cone and ring structure with the exception of having the subacrosomal cone in Ascaphus and the ring in Discohlossus as compared with those of urodeles. Persistence of the subacrosomal cone and the ring structure is typical in most urodeles and is further linked with the primitive anurans. Therefore, these characters are regarded as symplesiomorphies in urodeles and primitive anurans. The organization of sperm tail, endounclear canal and perforatorium indicates a close phylogenetic relationship between urodeles and the primitives anurans.

• The Korean Journal of Ecology
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• v.21 no.3
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• pp.269-276
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• 1998

The study of the Rana plancyi, was done during the calling period at a pond in Osong, Chungbuk, Korea from May to the middle of August of 1996 and 1997. Five basic types of call-A, B, C, D, E, - were identified in Rana plancyi according to the structure of call types. The structure of A type call has only one pulse, B type call has two pulses and C, D type call has one separated pulse which is called introductory call and pulse group as follow it. On the other hand, E type call has 3-6 separate pulses. The interval between introductory call and pulse group is $0.73{\pm}0.29$ s(n=159) in C type call and $0.60{\pm}0.21$ s (n=48) in D type call. The number of pulses in the pulse group is $30.08{\pm}8.69$ in C type call and $15.78{\pm}2.40$ in D type call. An increase in water temperature induces a decrease in C and D type call duration (C type call r= -0.4153, p<0.001, D type call r=-0.7064, p<0.001). In case of C and D type call, the interval between introductory call and pulse group influenced more call duration than pulse group duration. We regarded A, B type call as a territorial call and C, D type call as a mating call and we recognized that E type call has the function of threat or alarm.

• The Korean Journal of Zoology
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• v.3 no.2
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• pp.13-18
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• 1960

For the study on the sex ratio in natural populations of the different localities, the amphibian species were collected as the materials at the seven localities in Korea, and were obtained the following results. The sex ratios of each amphibian species suggest that each has it's own specific sex rations the following list. Rana nigromaculata H. : 109-39, Rana rugosa SCH. : 67.44, Rana temporaria coreana O. : 158.45, Hyla arborea japonica G. : 137.70. In Rana nigromaculata H. the average sex ratio was 109.39 as the above list, and its was reached statistically significant level according to the different localiies. The sex ratio was generally higher in the eastern area than that in the western area in this country. The sex ratio of the adult frogs of Rana nigromaculata H. was 135.18 , and it has a statistically significant level by each locality but the sex ratio of the younger frogs of the same species was 100.97 which was a little lower than that in the adult, and it was not laid at a significant level according to the localities. There is no doubt that the difference of sex ratios according to the localities was mainly due to the different ratios of the adult frogs which were effected on the constitution of sex by environmental factors in each locality.

• The Korean Journal of Zoology
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• v.33 no.1
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• pp.1-5
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• 1990

The comparative karyological analysis of the Korean treefrogs, Hyla japonica and Hyla suweonensis, were performed by C-banding method. Heteromorphic sex chromosomes, female heterogamety, has been identified in the 3rd chromosomes of H. suweonensis H. suweonensis seem to have sex chromosomes which are Zz/ZW type. The Z chromosomes contain large amount of constitutive heterochromatin, but little heterochromatin is located in the W chromosomes. This is in contrast to all previously known amphibian and most other vertebrate's W or Y chromosomes, except Gastrotheca oui'ern and G. walken (Schmid et al., 1988).

• Animal cells and systems
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• v.4 no.1
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• pp.31-37
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• 2000

The genetic variations among six species of Rana from Korea (R. nigro-maculata, R. piancyi, R. dybowskii, R. sp, R. rugosa type A, B and R. amurensis) were investigated using 499 bases of mitochondrial DNA sequences for ND2 (NADH dehydrogenase subunit 2) gene and $tRNA^{Trp}$ gene. Partial sequences of ND2 gene (427 bp) and full sequences of $tRNA^{Trp}$ gene (73 bp) were identified. The level of sequence divergences ranged from 0.2 to 5.2% within species and 4.9-28.0% among 6 species of the genus Rana. The $tRNA^{Trp}$ gene of the genus Rana was composed of 77 nucleotides which showed a two dimensional "cloverleaf" structure. The secondary structure of $tRNA^{Trp}$ was not found compensatory changes which could potentially confound phylogenetic inference. In the neighborjoining tree, brown frogs were clustered first with the level of sequence divergence of 13.20% between R. amurensis and R. dybowskii, and 9% between R. dybowskii and R. sp. supported by 99% bootstrap iterations, respectively. R. nigromaculata and R. plancyi were clustered into another group with 5.1% divergence supported by 100% bootstrap iteration. R. rugosa A 8nd B types were grouped by 4.9% divergence and clustered into the last group with other two groups with 100% bootstrap iterations.

• Animal cells and systems
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• v.4 no.1
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• pp.39-44
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• 2000

To assess the genetic diversity and population structure of Korean K. borealis, allozyme analysis was performed. The average genetic variability of Korean K. borealis populations was %P=13.2, Ho=0.048, and He=0.045. This value was the lowest in comparison with other Korean amphibian species studied. Also, the value was much lower than that of a reference population from Chinese K. borealis (%P=50, Ho=0.125, He=0.172). Wright's F-statistics showed that Korean K. borealis has distinctly low level of gene flow among regional populations (F$_{ST}$=0.339, Nm=0.487) in comparison with other Korean amphibian species studied. However, the average level of genetic divergence among Korean K. borealis populations was moderate (Nei's D=0.020). Therefore, it appeared that low levels of genetic diversity (He=0.045) and gene flow (Nm=0.487) among regional populations ave probably due to the results of decreasing population size and patchy distribution of this species in Korea.

• Fisheries and Aquatic Sciences
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• v.26 no.4
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• pp.241-255
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• 2023

The robust development of frog farming offered high economic benefits but created a large waste residue of frog bones and skin that received little attention. Over the years, inedible by-products have often been processed into biomolecules of potential value and environmental benefits, such as collagen, gelatin, and bioactive peptides. An overview of bioactive compounds on frog skins from various countries indicated that brevinin was the most abundant biological peptide found in frog skin. Other remaining compounds also possessed their highlighted activities, including antibacterial, stimulating insulin release and gastric hormone release, anti-cancer, and neuroregulatory. Notably, various components have been analyzed in the structure and sequence to give meaningful insight into clustering components related to their biological activity. This review may create a source of raw materials for the developmental research of by-products from frog skin and concomitantly reduce environmental pollution.