• Title/Summary/Keyword: Antioxidative enzyme

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Changes in ROS-Scavenging Enzyme Activity in Rice (Oryza sativa L.) Exposed to High Salinity

  • Koo, Jeung-Suk;Choo, Yeon-Sik;Lee, Chin-Bum
    • Journal of Ecology and Environment
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    • v.30 no.4
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    • pp.307-314
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    • 2007
  • We studied changes in the biochemical and physiological status and ROS-scavenging enzyme (superoxide dismutase, catalase and peroxidase) activity in leaves and roots of rice (Oryza sativa L.) plants exposed to high salinity. Under salt stress, the reduction in RWC (relative water content) in leaves was relatively severe in comparison with that of roots. The proline content was also significantly higher in leaves of rice plants following salt treatment. The activities of CAT and POX in roots increased with increasing NaCl concentration, but the activity of SOD decreased. These results suggest that the increase of endogenous proline is closely associated with the increase of CAT and POX activities, which may play important roles in salt tolerance. Therefore, we conclude that the alleviation of oxidative damage and increased resistance to salinity may result from the presence of efficient antioxidative systems.

Trends of Several Air Pollutants and the Effects of Ozone on the Plant Antioxidant system in Platanus occidentalis in Korea

  • Woo, Su-Young
    • Journal of Korean Society of Forest Science
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    • v.95 no.2
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    • pp.183-187
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    • 2006
  • This study investigated concentrations of the several air pollutants and compared antioxidative enzyme activities on Platanus occidentalis because this tree species is one of the widespread street trees in Korea. This species has been emerging the ambient air pollutants during its growing periods. The purpose of this study was to identify the relationship between air pollution on the tree species and antioxidant enzyme activities on the trees. $O_3$, $NO_2$, CO and $SO_2$ concentrations of several cities in Korea were compared for last decades. Among the air pollutants, $O_3$ and $NO_2$ concentrations in six big cities in Korea showed similar increasing trends during this period. In contrast, $SO_2$ and CO concentrations in the same cities dramatically decreased between 1994 and 2005. Platanus occidentalis trees were controlled to investigate, ascorbate peroxidase (APX) and glutathione reductase (GR) activity. Ozone exposure generally increased APX and GR activities of tree seedlings. It is a typical compensatory strategy of stressed trees.

Plasma Antioxidant Status and Platelet Antionxidative Enzyme Activities in Patients of Ischemic Heart Disease (허혈성심질환에서의 항산화영양소 상태와 혈소판 항산화계효소 활성에 관한 연구)

  • 최영선
    • Journal of Nutrition and Health
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    • v.29 no.2
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    • pp.223-231
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    • 1996
  • To investigate antioxidant status and platelet antioxidative enzyme activity in patients with ischemic heart disease, 36 male patients admitted to Kyungpook National University Hospital from June to December 1994 were compared to 36 healthy male control subjects. The percentages of heavy smoking and nonexercise were significantly higher in the patient group compared to the control, but the drinking status was not significantly different between groups. Food habit and food frequency scores were significantly lower in patients than in control subjects. Plasma retinol levels tended to be lower in the patient group, and plasma $\alpha$-tocopherol and $\beta$-carotene levels were not different between groups. There was no difference in the level of plasma thiobarbituric acid reactive substances(TBARS) and in the activities of platelet glutathione peroxidase and catalase. Our results indicate that oxidative stress, which is reflected by the plasma levels of antioxidants and TBARS, did not increase in the patients with ischemic heart disease, and the long-term effects due to smoking, poor food habit and other life styles could possibly contribute to the onset of the disease.

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Effect of Oolong Tea Extracts on Plasma Glucose Level and Antioxidant System in Diabetic Rats

  • Quan, Zhe-Jiu;Seo, Jung-Sook
    • Journal of Community Nutrition
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    • v.8 no.4
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    • pp.207-213
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    • 2006
  • The present study was conducted to investigate the effect of oolong tea extract on blood glucose level and antioxidant system in diabetic rats. The Sprague-Dawley rats were fed on AIN-76 based experimental diets containing 1 % oolong tea extract for 6 weeks. They were induced to be diabetic by receiving streptozotocin (45mg/kg BW) intramuscularly. Blood glucose, blood and hepatic concentration of vitamins A and E, and antioxidant enzyme activities were measured. Oolong tea extract feeding decreased the plasma glucose in diabetic rats. Dietary supplementation of oolong tea extract did not affect antioxidative enzyme activities such as superoxide dismutase, glutathione peroxidase and catalase in diabetic rats. The plasma level of retinol was increased in diabetic rats by feeding oolong tea extract. Plasma and hepatic levels of ${\alpha}$-tocopherol were higher in diabetic rats fed oolong tea extract. In conclusion, these results suggest that oolong tea extract consumption might reduce the plasma glucose in diabetic rats and protect the oxidative damage from diabetic stress to some extent.

The Effect of Tocopherol and Ascorbate on the Enzyme Activity and Antioxidation in $CCl_4$ Induced Rats (Tocopherol과 Ascorbate 투여가 사염화탄소로 유도한 흰쥐의 효소활성 및 항산화적용에 미치는 영향)

  • 하배진;이상현;하종명
    • Journal of Life Science
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    • v.9 no.3
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    • pp.300-307
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    • 1999
  • The enzyme activities and antioxidative of tocopherol and ascorbate were investigated at the levels of liver, kidney homogenates and sera of SD-rats intoxicated with tetrachlorocarbon$CCl_4$. GOT and GPT activities of sera in the $CCl_4$ group were 3, 6 times increased compared to normal group. But they tended to decrease significantly in tocopherol and ascorbate administered group. As for BUN and total cholesterol they were the same. HDL-cholesterol in the $CCl_4$group was 42% decreased compared to normal group. HDL-cholesterol was about 26% increased in the tocopherol group and tocopherol ascorbate group compared to $CCl_4$ group. MDA and SOD activities in the liver and the kidney tissue homogenates were significantly increased in $CCl_4$ group compared to normal group. But they were decreased significantly in the tocopherol group and tocopherol-ascorbate group compared to $CCl_4$ group. In view of this study tocopherol and ascorbated were ascorbate were effective on the detoxication of liver and kidney injury.

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Changes in Chemical Composition and Biological Activities of Oriental Crude Drugs by Food Processing Techniques (I) - Changes in Liquiritigenin Contents in Licorice Extract Treated by the Crude Enzyme Extract from Aspergillus kawachii (식품학적 가공에 의한 생약의 성분 및 활성 변화 (I) - Aspergillus kawachii 유래 조효소액 처리에 의한 감초 추출물 중 Liquiritigenin의 함량변화)

  • Kim, Sang-In;Kim, Ji-Eun;So, Jai-Hyun;Rhee, In-Koo;Chung, Shin-Kyo;Lee, Kyung-Bok;Yoo, Yung-Choon;Song, Kyung-Sik
    • Korean Journal of Pharmacognosy
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    • v.35 no.4 s.139
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    • pp.309-314
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    • 2004
  • By treating crude enzyme extract from Aspergillus kawachii, the liquiritigenin content in the licorice (Glycyrrhizae Radix) was significantly increased. The liquiritigenin content reached its maximum level (45.7 mg/g licorice extract) after 60 min of incubation with the crude enzyme extract at $37^{\circ}C$, while the inactivated crude enzyme treated control contained trace amount (about 0.11 mg/g) of liquiritigenin. The enzyme-treated licorice extract inhibited more than 50% DPPH radical at 100 ppm and this was about two times higher activity compared to the enzyme-untreated control.

Effect of Myricetin Combined with Vitamin C or Vitamin E on Antioxidant Enzyme System in Murine Melanoma Cells (B16F10 세포에서 Flavonoid인 Myricetin과 Vitamine C, Vitamine E의 병용 투여가 항산화 효소계에 미치는 영향)

  • Yu, Ji-Sun;Kim, An-Keun
    • Korean Journal of Pharmacognosy
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    • v.35 no.4 s.139
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    • pp.357-363
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    • 2004
  • Flavonoids are class of polyphenolic compounds widely distributed in the plant kingdom, which display a variety of biological activities, including antiviral, antithrombotic, antiiflammatory, antihistaminic, antioxidant and free-radical scavenging abilities. To determined flavonoid, myricetin in the presence of other antioxidants - vitamin C and vitamin E - can exert antioxidative properties not only directly by modulating the AOE system but also scavenging free radical, we investigated cell viability, antioxidant enzyme activities and ROS level in B16F10 murine melanoma cell. B16F10 cells were exposed to medium containing myricetin in the presence or absence of vitamin C or vitamin E for a period of 24 hr. Cell viability was measured by MTT assay. In co-treating myricetin with other antioxidants, CAT activities were increased, compared with control, but SOD and GPx activities were decreased, compared with each antioxidant treated groups . In the group of myricetin or myricetin present with other antioxidants, ROS levels were decreased dose-dependently. Especially, myricetin present of other antioxidants were decreased compared with myricetin.

Antioxidant and hepatoprotective action of the crude ethanolic extract of the flowering top of Rosa damascena

  • Alam, MA;Nyeem, MAB;Awal, MA;Mostofa, M;Alam, MS;Subhan, N;Rahman, M Mostafizur
    • Advances in Traditional Medicine
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    • v.8 no.2
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    • pp.164-170
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    • 2008
  • The hepatoprotective activity of the alcoholic extract of Rosa damascena was studied against paracetamol induced acute hepatotoxicity in rats. Liver damage was assessed by estimating serum enzyme activities of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and histopathology of liver tissue. Pre- and post-treatment with ethanolic extracts showed a dose-dependent reduction of paracetamol induced elevated serum levels of enzyme activity. The mechanism underlying the protective effects was assayed in vitro and the R. damascena extracts displayed dosedependent free radical activity using DPPH ($IC_{50}=162.525\;{\mu}g/ml$) and TBA method. The hepatoprotective action was confirmed by histopathological observation. The ethanolic extracts reversed paracetamol induced liver injury. These results suggest that the hepatoprotective effects of R. damascena extracts are related to its antioxidative activity.

Hair Growth Activity and Melanogenic Activity of Oriental Medical Prescription (한방복합처방단의 발모효과 및 멜라닌 생성 촉진효과)

  • Im, Kyung-Ran;Kim, Mi-Jin;Yoon, Kyung-Sup
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.37 no.2
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    • pp.161-169
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    • 2011
  • To develop a new natural cosmetic hair care product for scalp treatment, we investigated the effect of enzyme modified (Viscozyme, Pectinex) oriental medical prescriptions on hair growth and melanogenesis. Enzyme modified oriental medical prescriptions showed the antioxidative effect, inhibitory effects on lipoxygenase activity and $5{\alpha}$-reductase activity and melanogenic activity. In human irritation test, they did not show any adverse effect. Based on these results, we suggest that enzyme modified oriental medical prescriptions may potentially be used in a cosmeceutical hair care product for scalp treatment.

Physiological and biochemical responses of elevated ozone on Pterocarpus indicus under well-watered and drought conditions

  • Baek, Saeng Geul;Park, Jeong ho;Kwak, Myeong Ja;Lee, Jong Kyu;Na, Chae Sun;Lee, Byulhana;Woo, Su Young
    • Forest Science and Technology
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    • v.14 no.4
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    • pp.153-159
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    • 2018
  • Seedlings of Pterocarpus indicus were grown in both well-watered and drought stress conditions in phytotron. Seedlings grown under well-watered and drought stress conditions were exposed to either combined or without ozone of 200 ppb for one month. First, the physiological responses to elevated ozone levels indicated a decreased biomass. The seedlings grown in arid soil and exposed to ozone showed less biomass than those grown in arid soil but not exposed to ozone. Moreover, all the seedlings except the well-watered and unexposed ones showed a significantly lower photosynthetic rate ($P_N$) over time. However, with the accumulation of ozone injuries, the antioxidant enzyme activities increased overall. In the study results, when exposed to ozone, the well-watered seedlings exhibited more antioxidative enzyme activity than did the seedlings grown in arid soil. Generally, P. indicus in arid soil suffered less damage from elevated ozone than did the well-watered plants.