• Title/Summary/Keyword: Antioxidant index

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Screening of Natural Antioxidant from Plant and Their Antioxidative Effect (식물성 천연 항산화물질의 검색과 그 항산화력 비교)

  • Choi, Ung;Shin, Dong-Hwa;Chang, Young-Sang;Shin, Jae-Ik
    • Korean Journal of Food Science and Technology
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    • v.24 no.2
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    • pp.142-148
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    • 1992
  • Certain parts of 95 species of edible and medical plants were extracted with water and 75% of ethyl alcohol. After addition of those extracts to palm oil, lard and soybean oil at different level, their antioxidative activities were compared by Rancimat test. Six species among them seemed to have rather strong antioxidative activity and high extracting yields(i.e. Taraxacum platycarpum, Plantago asiatica, Rhus javanica L., Lycopus lucidus, Astragalus membranaceus, Taraxacum platycarpumH). Among them, the Rhus javanica L. ethanol extract retarded greatly the induction period of palm oil and lard. When 600 ppm of Rhus javanica L. extract were added to palm oil and lard, AI(antioxidant index was expressed as induction period of oil containing various plant extracts/induction period of control oil) of each was 1.35 and 3.03 respectively. This result indicated that the Rhus javanica L. extract was more effective on lard than the other oils.

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Physicochemical and Antioxidant Properties of Sponge Cake made using Barley Sprout and Green Tea (새싹보리와 녹차 스펀지케이크의 이화학적 및 항산화적 품질 특성)

  • Kim, Eunkyung
    • Journal of the Korean Society of Food Culture
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    • v.37 no.1
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    • pp.90-98
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    • 2022
  • This study investigated the physicochemical and antioxidant properties of sponge cakes made using different amounts (2 and 4%) of barley sprout powder (BSP) and green tea powder (GTP), respectively. The results showed that the baking loss rate of GS2 (2% green tea) and GS4 (4% green tea) was 12.39% and 11.96%, respectively in the green tea addition group, which was higher than that of the barley sprout group, but significantly lower than that of the control group at 13.34% (p<0.05). The specific volumes of the sponge cake containing barley sprout and green tea 2% and 4% were between 2.84-2.95 mL/g, which was significantly higher compared to the control group at 2.69 mL/g (p<0.05). The sugar content was significantly higher in the control group and the barley sprout addition group at 2.30°Brix (p<0.05). As for the volume index, the control group with the lowest value at 13.43 showed a significant difference compared to the addition groups. The volume index significantly decreased as the addition amount increased, measuring 14.07 in BS4 (4% BSP) compared to 14.87 of BS2 (2% BSP) in the barley sprout group (p<0.05). In cross-sectional photography, the color became darker than that of the control group as the quantum of additives increased. In terms of the DPPH and ABTS radical scavenging activity, the total phenol content, and total flavonoid content, the groups with the addition of barley sprout and green tea showed higher antioxidant activity than the control group (p<0.05).

Effect of Soy Isoflavone Supplementation on the Bone Mineral Density and Antioxidant Enzyme Activity in Postmenopausal Women (일부 폐경 후 여성에서 골밀도와 항산화효소 활성에 대한 대두 이소플라본 보충 효과)

  • Lee, Haeng-Shin;Lee, Da-Hong;Kim, Mi-Hyun;Sung, Chung-Ja
    • Journal of Nutrition and Health
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    • v.39 no.8
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    • pp.801-807
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    • 2006
  • To elucidate the effect of soy isoflavone supplementation on bone mineral density and antioxidant enzyme activity in 60 postmenopausal women residing in Iksan area were recruited. There were 31 participants in the treatment group and 29 in the control group. The treatment group consumed isoflavone extract capsules daily (which contained 90 mg of soy isoflavones) for 12 weeks. The study compared before and after isoflavone intake in the following areas. Physical examination, diet survey, bone mineral density (BMD) and antioxidant enzyme activity (superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and total antioxidant capacity (TA)). The average age of the treatment group was 64.6 years and that of the control group was 66.5 years. There were no significant differences between the two groups in terms of height, weight and body mass index. Both groups maintained a regular diet pattern in terms of their average daily nutrient intake. There were no significant differences between the treatment group (23.3 mg) and the control group (24.0 mg) in terms of daily isoflavone intake based on diet. Isoflavone supplementation of 12 weeks did not resulted in any significant changes in BMD or parameters of antioxidant enzyme activity, implying the necessity of more intensive intervention for a substantial change. In conclusion, this study revealed that antioxidant enzyme activity holds a significant relationship with the bone mineral density in postmenopausal women and further systematic research on dose and period of isoflavone supplementation is needed to clearify the positive effect of isoflavone on BMD and/or blood antioxidant capacity in postmenopausal women.

Antioxidant activity of the extracts of adzuki bean (Vigna angularis) landraces in Korea

  • Lee, Kyung Jun;Ma, Kyung-Ho;Cho, Yang-Hee;Lee, Jung-Ro;Chung, Jong-Wook;Lee, Gi-An
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.218-218
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    • 2017
  • Adzuki bean (Vigna angularis) has been extensively investigated due to their biological activities. In this study, total polyphenol content (TPC), total phenolic acid content (TPA), and total flavonoid content (TFC) in 209 Korean adzuki bean landraces were determined by colorimetric methods. Antioxidant effects were evaluated with the DPPH, ABTS, ferric reducing antioxidant power (FRAP), reducing power (RP), and SOD assays. TPC, TPA, and TFC in the 209 Korean adzuki bean landraces ranged from 1.1 to 11.7 mg gallic acid equivalents/g, 0.37 to 5.03 mg caffeic acid equivalents/g, and 0.17 to 0.91 mg quercetin equivalents/g, respectively. Antioxidant activities as assessed by the DPPH, ABTS, FRAP, PR, and SOD assays showed wide variation, ranging from 12.2 to 86.3 (IC50), 0.85 to 5.25 mg ascorbic acid equivalents (ASC)/g, 0.41 to 5.44 mg ASC/g, 0.54 to 1.83 mg ASC/g, and 60.4 to 142.8 (IC50), respectively. Using the relative antioxidant capacity index (RACI), we found that the IT189394 sample had the highest antioxidant activity. In clustering analysis, 209 Korean adzuki bean landraces were classified into three clusters. Among them, cluster I contained 22 accessions with higher antioxidant activities, TPC, TFC, and TPA and smaller seed sizes than the other clusters. We anticipate that these results will provide useful information for the development of adzuki bean-based functional foods.

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Comparison of Index Compounds Content and Antioxidative Activity of Wild Ginseng Pharmacopuncture by Extraction Methods (산양산삼약침의 추출법 별 성분 및 항산화 활성 비교)

  • Lee, Dae-yeon;Choi, Byoung-sun;Lee, In-hee;Kim, Jae-hyun;Gwon, Pil-seung
    • The Journal of Internal Korean Medicine
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    • v.39 no.3
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    • pp.313-322
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    • 2018
  • Objectives: Wild ginseng pharmacopuncture is widely used in oriental medicine. However, there is no standard method for efficiently extracting the active ingredient. In this study, in order to determine an efficient extraction method, wild ginseng was extracted by the distillation and 70% ethanol reflux methods, respectively. In comparing each extract, the index compounds were analyzed, and antioxidant activity was measured. Methods: The index compounds, ginsenoside Rg1 and ginsenoside Rb1, were detected using high performance liquid chromatography (HPLC). Antioxidative activities of total phenolic compounds, DPPH (${\alpha}$, ${\alpha}$-diphenyl-${\beta}$-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) and FRAP (ferric reducing antioxidant power) were measured to compare their bioactivities. Since saponin is known to be hemolytic, the hemolytic activity of each extract was compared. Results: The index compounds were analyzed. Nothing was detected in the wild ginseng distilled extracts (WGDE). In the wild ginseng 70% ethanol reflux extracts (WGEE), ginsenoside Rg1 was 3.66 mg/g, and ginsenoside Rb1 was 16.70 mg/g. WGEE showed higher levels than WGDE in all antioxidative activities. In the hemolytic test, the extracts showed almost no toxicity, but WGEE showed lower toxicity than WGDE. Conclusions: In this study, it was concluded that WGEE is more advantageous than WGDE in the detection of index compounds and bioactivity. However, additional studies of additional extraction methods and other bioactivity tests are needed.

Antioxidant Activity of Grape Seed Ethanol Extract (포도종실 에탄올 추출물의 항산화 활성)

  • 정하열;윤수정
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.5
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    • pp.893-898
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    • 2002
  • To examine the antioxidant activity of grape seed ethanol extract, the antioxidative index (AI) by the active oxygen method (AOM) and peroxide value (POV) of linoleic acids containing the extract at levels of 100, 500, 1000 ppm was measured during storage at 6$0^{\circ}C$ for 12 days. When comparing with BHT, the extract at levels of 500 ppm showed similar or better antioxidant activity (AI: 2.25, POV: 57 meq/kg oil) than that (AI: 1.21, POV:58 meq/kg oil ) of BHT at 200 ppm level. The mixture of 500 ppm of the extract and 500 ppm of ascorbic acid showed intense synergistic antioxidant activity (AI: 6.21, POV: 14 meq/kg oil) compared with using 1000 ppm of the extract only (AI: 3.39, POV 43 meq/kg oil). Also to determine the feasibility of using the extract for natural antioxidant, the oxidative stability of roasted peanut and Ramyon was investigated by measuring the POV of crude oils from the samples stored at 60"C for 18 days. The oxidative stability of roasted peanut and Ramyon seemed to be enhanced by treatment with the extract at level of 1000 ppm, especially with the 1 : 1 mixture of extract and ascorbic acid. This study suggested that grape seed ethanol extract could be used as the natural antioxidant for the improvement of overall oxidation stability of fat containing foods.oods.

Effect of Supplementation of Antioxidant Nutrient Against Oxidant Stress during Exercise

  • Kim, Hye-Yount
    • Journal of Nutrition and Health
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    • v.30 no.9
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    • pp.1061-1066
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    • 1997
  • This study was undertaken to evaluate the effect of 4 weeks of $\alpha$-tocopherol(800 I.U./d) supplementation on oxidant stress of eleven female aerobic -majoring students during rest and exercise. Changes in the activity of the antioxidant enzyme glutathione peroxidase were also studied. Serum $\alpha$-tocopherol concentration was significantly increased with vitamin E supplementation(710.1$\pm$113.8$\mu\textrm{g}$/dl vs. 1,485,8$\pm$105.2$\mu\textrm{g}$/dl). In addition, serum MDA concentration, an index of lipid peroxidation, significantly decreased after vitamin E supplementation. However, MDA values after exercise increased to pre-supplementation levels. Serum glutathione peroxidase activity significantly increased with vitamin E supplementation. The enzyme activity showed a trend toward decrease after exercise. Serum cholesterol values were not significantly affected by vitamin E supplementation. However, serum triglycerides significantly increased after supplementation against oxidative stress during resting periods. These supplements appraently work by decreasing lipid peroxidation and increasing glutathione peroxidase activity. However, vitamin E supplementation did not prevent exercise-induced increases in lipid peroxidation.

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Volatile Compound, Physicochemical, and Antioxidant Properties of Beany Flavor-Removed Soy Protein Isolate Hydrolyzates Obtained from Combined High Temperature Pre-Treatment and Enzymatic Hydrolysis

  • Yoo, Sang-Hun;Chang, Yoon Hyuk
    • Preventive Nutrition and Food Science
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    • v.21 no.4
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    • pp.338-347
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    • 2016
  • The present study investigated the volatile compound, physicochemical, and antioxidant properties of beany flavor-removed soy protein isolate (SPI) hydrolyzates produced by combined high temperature pre-treatment and enzymatic hydrolysis. Without remarkable changes in amino acid composition, reductions of residual lipoxygenase activity and beany flavor-causing volatile compounds such as hexanol, hexanal, and pentanol in SPI were observed after combined heating and enzymatic treatments. The degree of hydrolysis, emulsion capacity and stability, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, and superoxide radical scavenging activity of SPI were significantly increased, but the magnitudes of apparent viscosity, consistency index, and dynamic moduli (G', G") of SPI were significantly decreased after the combined heating and enzymatic treatments. Based on these results, it was suggested that the enzymatic hydrolysis in combination with high temperature pre-treatment may allow for the production of beany flavor-removed SPI hydrolyzates with superior emulsifying and antioxidant functionalities.

Physicochemical Characteristic of Ultrafine Sparassis crispa(cauliflower mushroom) Powder

  • Sun-Sun Hur
    • Journal of the Korean Applied Science and Technology
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    • v.40 no.5
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    • pp.945-954
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    • 2023
  • In this study, Sparassis crispa(cauliflower mushroom), which is rich in beta-glucan, was pulverized using ultrafine grinding technology for its potential utilization as a diverse food ingredient. The physical and antioxidant properties of cauliflower mushroom powder were evaluated at various grinding times. The results showed that as the grinding time of cauliflower mushroom increased, the average particle size significantly decreased (p<0.05). Additionally, the water-holding capacity, swelling capacity, and water solubility index of cauliflower mushroom increased significantly(p<0.05). Based on the analysis mentioned above, cauliflower mushroom prepared as a superfine powder for 5 minutes exhibited superior physical and chemical properties as well as antioxidant characteristics and is expected to be widely used in various foods.

Antioxidant and Biological Activity in the Leaves of Adzuki Bean (Vigna angularis L.)

  • Lee, Kyung Jun;Lee, Jung-Ro;Shin, Myoung-Jae;Cho, Gyu-Taek;Lee, Ho-Sun;Ma, Kyung-Ho;Lee, Gi-An;Chung, Jong-Wook
    • Korean Journal of Plant Resources
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    • v.31 no.3
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    • pp.237-253
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    • 2018
  • The adzuki bean (Vigna angularis L.) is a red-grained legume that has a number of essential nutrients and is used in traditional dishes in Asia. Adzuki bean industrial by-products are also a potential low-cost source of some unique bioactive polyphenols. Hence, here, the authors aimed to perform a comparative study of the phytochemical profiles of the leaves and seeds of the adzuki bean and compare their antioxidant, ${\alpha}$-glucosidase inhibition, and tyrosinase inhibition activity. The authors assessed antioxidant activity by DPPH, ABTS, FRAP, PR, TPC, and SOD assays, which showed wide variation, respectively. From the relative antioxidant capacity index results, 10 adzuki bean landraces were selected to compare for phytochemicals and bioactivity using leaf and seed extracts. Antioxidant, ${\alpha}$-glucosidase inhibition, and tyrosinase inhibition activity in the leaf extracts were higher than in the seed extracts, and there were more flavonols and isoflavones in the leaf extracts than in the seed extracts. This study demonstrated that adzuki bean leaf extracts could be a new natural antioxidant or antidiabetic agent and a skin whitener and can also be used in industrial applications.