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http://dx.doi.org/10.7732/kjpr.2018.31.3.237

Antioxidant and Biological Activity in the Leaves of Adzuki Bean (Vigna angularis L.)  

Lee, Kyung Jun (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA)
Lee, Jung-Ro (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA)
Shin, Myoung-Jae (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA)
Cho, Gyu-Taek (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA)
Lee, Ho-Sun (International Technology Cooperation Center, RDA)
Ma, Kyung-Ho (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA)
Lee, Gi-An (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA)
Chung, Jong-Wook (Department of Industrial Plant Science & Technology, Chungbuk National University)
Publication Information
Korean Journal of Plant Resources / v.31, no.3, 2018 , pp. 237-253 More about this Journal
Abstract
The adzuki bean (Vigna angularis L.) is a red-grained legume that has a number of essential nutrients and is used in traditional dishes in Asia. Adzuki bean industrial by-products are also a potential low-cost source of some unique bioactive polyphenols. Hence, here, the authors aimed to perform a comparative study of the phytochemical profiles of the leaves and seeds of the adzuki bean and compare their antioxidant, ${\alpha}$-glucosidase inhibition, and tyrosinase inhibition activity. The authors assessed antioxidant activity by DPPH, ABTS, FRAP, PR, TPC, and SOD assays, which showed wide variation, respectively. From the relative antioxidant capacity index results, 10 adzuki bean landraces were selected to compare for phytochemicals and bioactivity using leaf and seed extracts. Antioxidant, ${\alpha}$-glucosidase inhibition, and tyrosinase inhibition activity in the leaf extracts were higher than in the seed extracts, and there were more flavonols and isoflavones in the leaf extracts than in the seed extracts. This study demonstrated that adzuki bean leaf extracts could be a new natural antioxidant or antidiabetic agent and a skin whitener and can also be used in industrial applications.
Keywords
Adzuki bean leaves; Antioxidant; By-products; ${\alpha}$-glucosidase inhibition activity; Tyrosinase inhibition activity;
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1 Acar, O.C., V. Gokmen, N. Pellegrini and V. Fogliano. 2009. Direct evaluation of the total antioxidant capacity of raw and roasted pulses, Nuts and Seeds. Eur. Food Res. Technol. 229:961-969.   DOI
2 Allard, R.W. 1996. Genetic basis of the evolution of adaptedness in plants. Euphytica 92:1-11.   DOI
3 Andrade-Cetto, A., J. Becerra-Jimenez and R. Cardenas-Vazquez. 2008. Alfa-glucosidase-inhibiting activity of some Mexican plants used in the treatment of type 2 diabetes. J. Ethnopharmacol. 116:27-32.   DOI
4 Archana, P., T. TSamatha, B. Mahitha, Chamundeswari and N. RamaSwamy. 2012. Preliminary phytochemical screening from leaf and seed extracts of Senna Alata L. roxb-an ethnomedicinalplant. Int. J. Pharm. Biol. Res. 3:82-88.
5 Bansal, S., S. Choudhary, M. Sharma, S.S. Kumar, S. Lohan, V. Bhardwaj, N. Syan and S. Jyoti. 2013. Tea: A native source of antimicrobial agents. Food Res. Int. 53:568-584.   DOI
6 Bernardi, A.P.M., J.D. Nunes, M.K. Marchioro, L.M.G. Rosa, G.L. von Poser and S.B. Rech. 2008. Phenolic compounds profiles during ex vitro acclimatization of micropropagated Hypericum polyanthemum. Plant Physiol. Bioch. 46:694-700.   DOI
7 Brush, S.B. 1995. In-situ conservation of landraces in centers of crop diversity. Crop Sci. 35:346-354.   DOI
8 Cazarolli, L.H., P. Folador, M.G. Pizzolatti and F.R.M.B. Silva. 2009. Signaling pathways of kaempferol-3-neohesperidoside in glycogen synthesis in rat soleus muscle. Biochimie 91: 843-849.   DOI
9 Chang, T.S., H.Y. Ding, S.S.K. Tai and C.Y. Wu. 2007. Mushroom tyrosinase inhibitory effects of isoflavones isolated from soygerm koji fermented with Aspergillus Oryzae Bcrc 32288. Food Chem. 105:1430-1438.   DOI
10 Chiavaroli, V., C. Giannini, S. De Marco, F. Chiarelli and A. Mohn. 2011. Unbalanced oxidant-antioxidant status and its effects in pediatric diseases. Redox Rep. 16:101-107.   DOI
11 Croteau, R., T.M. Kutchan and N.G. Lewis. 2000. Natural products (secondary metabolites): In Buchanan, B., W. Gruissem and R. Jones (eds.), Biochemistry and Molecular Biology of Plants, American Society of Plant Physiologists 24:1250-1319.
12 Fang, Y.Z., S. Yang and G.Y. Wu. 2002. Free radicals, antioxidants, and nutrition. Nutrition 18:872-879.   DOI
13 Fontana, Pereira D., L.H. Cazarolli, C. Lavado, V. Mengatto, M.S.R.B. Figueiredo, A. Guedes, M.G. Pizzolatti and F.R.M.B. Silva. 2011. Effects of flavonoids on $\alpha$-glucosidase activity: Potential targets for glucose homeostasis. Nutrition 27:1161-1167.   DOI
14 Han, K.H., T. Kitano-Okada, J.M. Seo, S.J. Kim, K. Sasaki, K. Shimada and M. Fukushima. 2015. Characterisation of anthocyanins and proanthocyanidins of adzuki bean extracts and their antioxidant activity. J. Funct. Foods 14:692-701.   DOI
15 Frankel, O., A.D.H. Brown and J.J. Burdon. 1995. The conservation of plant biodiversity. Cambridge University Press, Cambridge, UK.
16 Gokmen, V., A. Serpen and V. Fogliano. 2009. Direct measurement of the total antioxidant capacity of foods: The 'Quencher' approach. Trends Food Sci. Technol. 20:278-288.   DOI
17 Gohara, A.K., A.H.P. de Souza, S.T.M. Gomes, N.E. de Souza, J.V. Visentainer and M. Matsushita. 2016. Nutritional and bioactive compounds of adzuki bean cultivars using chemometric approach. Cienc. Agrotec. 40:104-113.   DOI
18 Hoisington, D., M. Khairallah, T. Reeves, J.-M. Ribaut, B. Skovmand, S. Taba and M. Warburton. 1999. Plant genetic resources: What can they contribute toward increased crop productivity? Proc. Natl. Acad. Sci. 96:5937-5943.   DOI
19 Kim, J.-S., C.-S. Kwon and K.H. Son. 2000. Inhibition of alpha-glucosidase and amylase by luteolin, a flavonoid. Biosci. Biotechnol. Biochem. 64:2458-2461.   DOI
20 Jarvis, D.I., A.H.D. Brown, P.H. Cuong, L. Collado-Panduro, L. Latournerie-Moreno, S. Gyawali, T. Tanto, M. Sawadogo, I. Mar, M. Sadiki, N.T.N. Hue, L. Arias-Reyes, D. Balma, J. Bajracharya, F. Castillo, D. Rijal, L. Belqadi, R. Ranag, S. Saidi, J. Ouedraogo, R. Zangre, K. Rhrib, J.L. Chavez, D.J. Schoen, B. Sthapit, P. De Santis, C. Fadda and T. Hodgkin. 2008. A global perspective of the richness and evenness of traditional crop-variety diversity maintained by farming communities. Proc. Natl. Acad. Sci. USA 105:5326-5331.   DOI
21 Kim, S. and H. Cha. 2017. Comparison of the total phenolic and flavonoid contents and antioxidant activities of four kinds of sand dune plants living in taean, Korea. Korean J. Plant Res. 30:8-16.   DOI
22 Kim, J., J. Hong, H.K. Jung, Y.S. Jeong and K. Cho. 2012. Grape skin and loquat leaf extracts and acai puree have potent anti-atherosclerotic and anti-diabetic activity in vitro and in vivo in hypercholesterolemic zebrafish. Int. J. Mol. Med. 30:606-614.   DOI
23 Kim, M.-H. and H.-K. Chung. 2013. Review of food therapy and development of diet therapy program for diabetes mellitus in Sikryochanyo. J. Kor. Soc. Diet. Cult. 28: 562-575.   DOI
24 Kim, S.M, J.H. Park, H.O. Boo, S.G. Song and H.Y. Park. 2017. In vitro comparision of biological activities of solvent fraction extracts from Orostachys japonicus. Korean J. Plant Res. 30:133-143.   DOI
25 Koh, E., K.M.S. Wimalasiri, A.W. Chassy and A.E. Mitchell. 2009. Content of ascorbic acid, quercetin, kaempferol and total phenolics in commercial broccoli. J. Food Compos. Anal. 22:637-643.   DOI
26 Maynard, M., D. Gunnell, P. Emmett, S. Frankel and G.D. Smith. 2003. Fruit, vegetables, and antioxidants in childhood and risk of adult cancer: The Boyd Orr cohort. J. Epidemiol. Commun. H. 57:218-225.   DOI
27 Kubo, I., I. Kinst-Hori, S.K. Chaudhuri, Y. Kubo, Y. Sanchez and T. Ogura. 2000. Flavonols from heterotheca inuloides: Tyrosinase inhibitory activity and structural criteria. Bioorg. Med. Chem. 8:1749-1755.   DOI
28 Lee, D.J. and J.Y. Lee. 2004. Antioxidant activity by DPPH assay. Korean J. Crop Sci. 49:187-194.
29 Luo, J.Q., W.X. Cai, T. Wu and B.J. Xu. 2016. Phytochemical distribution in hull and cotyledon of adzuki bean (Vigna Angularis L.) and mung bean (Vigna radiate L.), and their contribution to antioxidant, anti-inflammatory and anti-diabetic activities. Food Chem. 201:350-360.   DOI
30 Matsuda, H., M. Higashino, Y. Nakai, M. Iinuma, M. Kubo and F.A. Lang. 1996. Studies of cuticle drugs from natural sources. IV. Inhibitory effects of some arctostaphylos plants on melanin biosynthesis. Biol. Pharm. Bull. 19:153-156.   DOI
31 Middleton, E., C. Kandaswami and T.C. Theoharides. 2000. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacol. Rev. 52:673-751.
32 Moon, H.-K., Y.-J. Lee, M.-R. Park and G.-Y. Kim. 2015. Development of native local foods in Sangju by storytelling-combined - a Case of 'General Jeong's Table'. J. Kor. Soc. Food Cult. 30:562-575.   DOI
33 Nanna, R.S., M. Banala, A. Pamulaparthi, A. Kurra and S. Kagithoju. 2013. Evaluation of phytochemicals and fluorescent analysis of seed and leaf extracts of Cajanus Cajan L.. Int. J. Pharm. Sci. Rev. Res. 22:11-18.
34 Patil, A.B. and A.S. Jadhav. 2013. Flavonoids an antioxidants: A review. Int. J. Pharm. Biol. Sci. Res. Develop. 2:7-20.
35 Nijveldt, R.J., E.L.S. Van Nood, D.E. Van Hoorn, P.G. Boelens, K. Van Norren and P.A.M. Van Leeuwen. 2001. Flavonoids: A review of probable mechanisms of action and potential applications. Am. J. Clin. Nutr. 74:418-425.   DOI
36 Oleszek, W. and A. Stochmal. 2002. Triterpene saponins and flavonoids in the seeds of Trifolium species. Phytochemistry 61:165-170.   DOI
37 Park, Y.M., J.B. Jeong, J.H. Seo, J.H. Lim, H.J. Jeong and E.W. Seo. 2011. Inhibitory effect of red bean (Phaseolus angularis) hot water extracts on qxidative DNA and cell damage. Korean J. Plant Res. 24: 130-138.   DOI
38 Sun, T. and S.A. Tanumihardjo. 2007. An integrated approach to evaluate food antioxidant capacity. J. Food Sci. 72:R159-R165.   DOI
39 Stanojevic, L., M. Stankovic, V. Nikolic, L. Nikolic, D. Ristic, J. Canadanovic-Brunet and V. Tumbas. 2009. Antioxidant activity and total phenolic and flavonoid contents of Hieracium Pilosella L. extracts. Sensors-Basel 9:5702-5714.   DOI
40 Sthapit, B.R. and V.R. Rao. 2009. Consolidating Community's role in local crop development by promoting farmer innovation to maximise the use of local crop diversity for the well-being of people. Acta. Hortic. 806:669-676.
41 Tadera, K., Y. Minami, K. Takamatsu and T. Matsuoka. 2006. Inhibition of alpha;-glucosidase and alpha-amylase by flavonoids. J. Nutr. Sci. Vitaminol. 52:149-153.   DOI
42 Terashima, M., A. Fukukita, R. Kodama, H. Miki, M. Suzuki, M. Ikegami, N. Tamura, A. Yasuda, M. Morikawa and S. Matsumura. 2013. Evaluation of antioxidant activity of leafy vegetables and beans with myoglobin method. Plant Cell Rep. 32:349-357.   DOI
43 Vilaro, N., M. Rebuffo, C. Miranda, C. Pritse and T. Abadie 2004. Characterization and analysis of a collection of Avena Sativa L. from Uruguay. PGR Newsletters FAO-Bioversity 140:23-31.
44 Waterhouse, A.L. 2001. Determination of total phenolics. In: Current protocols in food analytical chemistry. John Wiley & Sons, Inc.
45 Sabe, A.B., O.M. Onakoya and A.A. Oyagbemi. 2012. Hepatoprotective and in vivo antioxidant activities of ethanolic extract of whole fruit of Lagenaria Breviflora. J. Basic Clin. Physiol. Pharm. 23:27-32.
46 Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26:1231-1237.   DOI
47 Rho, C.W., S.Y. Son, S.T. Hong, K.H. Lee and I.M. Ryu. 2003. Agronomic characters of Korean adzuki beans (Vigna angularis (Willd.) Ohwi & Ohashi). Korean J. Plant. Res. 16:147-154
48 Robak, J. and R.J. Gryglewski. 1988. Flavonoids are scavengers of superoxide anions. Biochem. Pharmacol. 37:837-841.   DOI
49 Salah, N., N.J. Miller, G. Paganga, L. Tijburg, G.P. Bolwell and C. Riceevans. 1995. Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants. Arch. Biochem. Biophy. 322:339-346.   DOI
50 Wildmann, R. 2001. Nutraceuticals: A Brief review of historical and teleological aspects handbook of nutraceuticals and functional foods, CRC Press, Boca Raton:1-12.
51 Winkel-Shirley, B. 2001. Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol. 126:485-493.   DOI
52 Winkel-Shirley, B. 2002. Biosynthesis of flavonoids and effects of stress. Curr. Opin. Plant Biol. 5:218-223.   DOI
53 Singh, V., N. Guizani, M.M. Essa, F.L. Hakkim and M.S. Rahman. 2012. Comparative analysis of total phenolics, flavonoid content and antioxidant profile of different date varieties (Phoenix dactylifera L.) from Sultanate of Oman. Int. Food Res. J. 19:1063-1070.
54 Serpen, A., E. Capuano, V. Fogliano and V. Gokmen. 2007. A new procedure to measure the antioxidant activity of insoluble food components. J. Agr. Food Chem. 55:7676-7681.   DOI
55 Serpen, A., V. Gokmen, N. Pellegrini and V. Fogliano. 2008. Direct measurement of the total antioxidant capacity of cereal products. J. Cereal Sci. 48:816-820.   DOI
56 Shaik, S., N. Singh and A. Nicholas. 2011. Comparison of the selected secondary metabolite content present in the cancer-bush Lessertia (Sutherlandia) frutescens L. extracts. Afr. J. Tradit. Complement.Altern. Med. 8:429-434.
57 Zou, Y.P. and K.C. ChangSam. 2014. Antioxidant and anti- proliferative properties of extract and fractions from small red bean (Phaseolus Vulgaris L.). J. Food Nutr. 1:1-11.
58 Woo, K.S., S.B. Song, J.Y. Ko, Y.-B. Kim, W.H. Kim and H.-S. Jeong. 2016. Antioxidant properties of adzuki beans, and quality characteristics of sediment according to cultivated methods. Kor. J. Food Nutr. 29:134-143.   DOI
59 Yen, G.C. and P.D. Duh. 1993. Antioxidative properties of methanolic extracts from peanut hulls. J. Am. Oil Chem. Soc. 70:383-386.   DOI
60 Zhang, J., Y. Liu, J. Lv and G. Li. 2015. A colorimetric method for $\alpha$-glucosidase activity assay and its inhibitor screening based on aggregation of gold nanoparticles induced by specific recognition between phenylenediboronic acid and 4-aminophenyl-Α-D-glucopyranoside. Nano Res. 8:920-930.   DOI