• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.74-74
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• 2018

Abeliophyllum distichum is Korea Endemic Plants and its genetic resources found from Korea only. Bioactivities of A. distichum such as antioxidant, anti-cancer, and anti-inflammatory studies have been proved through many researches. Whereas, there are no studies on the biological activity of its callus extracts. In this study, we investigated the antioxidant activities of callus extracts derived from A. distichum and its inhibitory effect on oxidative DNA damage. The antioxidant activities were assessed using radical scavenging assays with DPPH, ABTS, and reducing power assay and the inhibitory effects on oxidative DNA damage were measured using ${\varphi}-174$ RF I plasmid DNA cleavage assay. In addition, callus extracts derived from A. distichum showed high antioxidant acitivties and no cytotoxicity in NIH/3T3. Also, it has significantly suppressed expression of ${\gamma}$-H2AX and p53 protein and mRNA levels in NIH/3T3 cells exposed to oxidative stress. Therefore, the callus extracts derived from A. distichum has potential antioxidant activity that can provide protective effects against the oxidative DNA damage caused by free radicals. This study suggest that it is valuable as cosmetics and medicine for antioxidant and cancer preventive materials.

• Journal of Applied Biological Chemistry
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• v.57 no.2
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• pp.123-129
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• 2014

The antioxidant and antimicrobial activities of Camellia oleifera seed oil were studied. Four kinds of seed oil samples were prepared, crude oil and refined oil, extracted by cold pressing method (CPC, CPR), and organic solvent extraction (OSC, OSR). Antioxidant activity analysis was measured in 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt, ferric reducing Ability of Plasma, and 2,2-diphenyl-1-picrylhydrazyl assays. Besides, the percentage of inhibition of red blood cells hemolysis induced by 2,2'-azobis(2-amidnopropane) dihydrochlorid, the lag time of LDL conjugated dienes formation in vitro, and the inhibitors of loss in tryptophan fluorescence were all used to estimate the antioxidant activity of the samples. The total phenolic contents (TPC) were detemined by Folin-Ciocalteu method. The TPC of the C. oleifera seed oils can be arranged in descending order: CPC ($1.9172{\mu}g/mL$) > OSC ($1.5218{\mu}g/mL$) > CPR ($1.0611{\mu}g/mL$) > OSR ($0.6782{\mu}g/mL$). And the oils were investigated for activity against Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger. The results showed the antioxidant activity of crude oil by cold pressing method was stronger than others, and all oils did inhibit activity of the top three bacteria expert A. niger. The further significance of the study contributes to measure the antioxidant and antimicrobial activity of the potential health benefits by the different methods of preparation and the oil of C. oleifera seeds acting as free radical scavenger, pharmaceuticals and preservatives may offer some information in medicine and cosmetic not just in food field.

• Journal of the Korean Applied Science and Technology
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• v.35 no.4
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• pp.1081-1087
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• 2018

This study was carried out to investigate the effects of the extracts of 80% EtOH and EtOAc, BuOH, and D.W in Korean native Hypsizygus marmoreus. Antioxidation and antioxidant analysis to provide the possibility of a multifunctional cosmetic raw material. As a result of analyzing the cytotoxicity of the sample using the animal cell line HaCaT cell, it was confirmed that the toxicity of the sample hardly affected the cells. The inhibition rate of elastase was 30.41% at 10% of sample concentration and inhibition activity of collagenase was 11.65% at 1% of sample concentration. Hypsizygus marmoreus showed better antioxidant activity than similar mushroom. The total phenol contents of the fractions were 778.4 mg and 2.59 mg per 1 g of white mushroom, confirming its potential as a natural antioxidant. As a result, the extract of Hypsizygus marmoreus is considered to be highly valuable as a natural cosmetic material having antioxidant and antioxidant functions.

• Journal of Microbiology and Biotechnology
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• v.33 no.8
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• pp.1030-1038
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• 2023

Lactiplantibacillus plantarum, previously named Lactobacillus plantarum, is a facultative, homofermentative lactic acid bacterium widely distributed in nature. Several Lpb. plantarum strains have been demonstrated to possess good probiotic properties, and Lpb. plantarum HOM3204 is a potential probiotic strain isolated from homemade pickled cabbage plants. In this study, whole-genome sequencing was performed to acquire genetic information and predict the function of HOM3204, which has a circular chromosome of 3,232,697 bp and two plasmids of 48,573 and 17,060 bp, respectively. Moreover, various oxidative stress-related genes were identified in the strain, and its antioxidant activity was evaluated in vitro and in vivo. Compared to reference strains, the intracellular cell-free extracts of Lpb. plantarum HOM3204 at a dose of 10¹⁰ colony-forming units (CFU)/ml in vitro exhibited stronger antioxidant properties, such as total antioxidant activity, 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate, superoxide dismutase activity, and glutathione (GSH) content. Daily administration of 10⁹ CFU Lpb. plantarum HOM3204 for 45 days significantly improved the antioxidant function by increasing the glutathione peroxidase activity in the whole blood and GSH concentration in the livers of D-galactose-induced aging mice. These results suggest that Lpb. plantarum HOM3204 can potentially be used as a food ingredient with good antioxidant properties.

• IMMUNE NETWORK
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• v.9 no.5
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• pp.147-152
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• 2009

Ascorbate (vitamin C) is a cofactor for a number of metabolic enzymes and is an indisputable essential vitamin C for humans. However, the potential of ascorbate as an anticancer agent has been a topic of controversy. A number of previous reports have addressed both positive aspects and limitations of ascorbate in cancer therapy. In this review, we briefly summarize the potential antitumor effects of ascorbate and its prospects for clinical use.

• Natural Product Sciences
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• v.13 no.3
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• pp.220-224
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• 2007

To search of more selective vasculogenic relaxation activity, the antioxidant activity of silkworm male pupae was determined by measuring its radical scavenging effect on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and anticoagulant activity of them was measured clotting time in both activated partial thromboplastin time (aPTT). Because, most of cGMP-enhancing agent such as, sildenafil, promotes thrombin-induced platelet aggregation, developed unexplained thrombic conditions including heart attack. To search more suitable and safe drug for vasculogenic relaxation, we purified silkworm pupae male extract. The ethyl acetate extract of silkworm male pupae showed strong scavenging activity in both DPPH and aPTT anticoagulant activity. The antioxidant activity potential of the individual fraction was in order of ethyl acetate > n-butanol > chloroform > n-hexane. The ethyl acetate soluble fraction exhibiting strong anti-oxidant and anticoagulant activity was further purified by repeated silica gel and Sephadex LH-20 column chromatography. Cholesterol was isolated as one of the active principles from ethyl acetate fraction, together with, minor portion, ${\beta}-sitosterol$.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.6
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• pp.471-475
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• 2004

The Oligomeric proanthocyanidin (OPC) in green and black tea, grape seeds, grapes and wine has raised much attention but that OPC in wild grape seed remains to be intensively investigated. This study investigated the total OPC contents and total antioxidant activity of wild grape seeds and developed an efficient extraction process with various temperatures, solvent compositions and times. Also, a chromatography column packed with the Dia-ion HP-20 resin was used for further purification of the ope. The total OPC contents were determined with the Folin-Ciocalteu reagent, and the antioxidant activity using total antioxidant potential (TAP) and 1,1-dipheny|-2picrylhydrazy| (DPPH). The yield of final purified OPC was 1.78 (+)-catechin equivalent (CE) g/100 g, with $IC_{50}$ activities of TAP and DPPH of 31.60 and $15.70\;{\mu}\;g/mL$. These activi­ties of the final purified OPC were about two times higher than that of the BHA used as a refer­ence sample.

• Food Science and Biotechnology
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• v.15 no.5
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• pp.710-714
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• 2006

The present study examined the lipid lowering and antioxidant activity of Paecilomyces tenuipes. Eight week-old male Sprague-Dawley rats were fed one of the three diets, a reference diet without cholesterol addition (NC), a high fat (17 g/ 100 g)-high cholesterol (1 g/100 g) diet (HC) and a HC diet supplemented with 3% P. tenuipes (PT) for 30 days. Total lipid and total cholesterol were reduced significantly by 33 and 37%, respectively, in the PT diet group compared with controls. A similar reduction was found for low density lipoprotein (LDL) cholesterol levels, while plasma high density lipoprotein (HDL) cholesterol and triglyceride (TG) concentrations were not significantly different among groups. Hepatic total lipid and total cholesterol levels, but not hepatic TG levels, were significantly decreased in the PT group compared to the HC group. The administration of P. tenuipes increased the plasma total antioxidant potential and decreased the levels of lipid peroxidation. These results suggest that P. tenuipes exerts significant health benefits through the modulation of physiological functions including a variety of atherogenic lipid profiles and antioxidants in hypercholesterolemia.

• The Korean Journal of Community Living Science
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• v.15 no.4
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• pp.11-16
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• 2004

This study was conducted to find the powerful free radical scavenging effect in methanol extracts from 35 kinds of Korean medicinal plants obtained from the Plant Extract Bank as natural plant products. Antioxidant activity of the plant extracts was determined by measuring the scavenging effect of 1,1-diphenyl-2-picryl hydrazyl radical (DPPH). In the screening of plant extracts tested, at 200 ppm level, almost all plantex tracts showed significantly high antionidant activity. In the reinvestigation of 15 kinds of Korean medicinal plants at 50 ppm level selected as good sources with good free radical scavenging effect at 200 ppm level, the DPPH scavenging effects were similar to those of L-ascorbic acid in whole plant extract of Fragaria yezoensis (90.4％), in the plant stems and roots extract of Gingko biloba, and the leaves extract of Rhododendron mucronulatum var. ciliatum (90％). The fruits extract of Cornus officinalis showed the strongest antioxidant activity (95％). These results suggest that the methanol extracts from Korean medicinal plants can be a potential source of antioxidants that can be used to control oxidative stress in the body, But further study is needed to measure their safety for consumption

• Journal of Microbiology and Biotechnology
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• v.10 no.6
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• pp.851-857
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• 2000

The activities of phase II and antioxidant enzymes in the liver, lung, kidney, stomach, and colon of mice were examined following intragastric application of polysaccharides extracted from soybeans fermented with either Agrocybe Cylindracea (AC) or Phellinus ignarius (PI). The intragastric application of the extracts to mice for 14 days significantly increased the activities of quinone reductase (QP) and glutathione S-transferase (GST) in the liver and kidney, glutathione (GSH) and superoxide dismutase (SOD) in the liver, kidney, lung, and stomach, and glutathione peroxidase (GSH-Px) in the liver, lung, and kidney. In general, the elevation of the phase II and antioxidant enzymes activities was more pronounced in the liver and kidney as compared to the lung, stomach, and colon. Accordingly, these finding suggest that polysaccharides extracted from soybeans fermented with A. cylindracea or P. igniarius have a cancer chemopreventive potential in various target organs.