• Title/Summary/Keyword: Antimicrobial test

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Evaluatioon of EEc 4-Plate Test for the Sensitivity and Identification of Families of Antimicrobial Drugs in Mea (EEC 4-Plate Test의 식육중 항균물질 검출감도와 항균물질 계열별 검출능 비교 조사)

  • 조병훈;진남섭;손성완;강환구;이혜숙;김재학;김봉환
    • Journal of Food Hygiene and Safety
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    • v.11 no.4
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    • pp.307-314
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    • 1996
  • The European Economic Community four plate test(EEC 4-plate test, FPT, EU) has been used for monitoring antimicrobial drug residues in meat by Local Veterinary Service Center in Korea. This study was performed to evaluate sensitivity and group specificity of some antimicrobial drugs in FPT and to compare FPT with Charm II test. The minimal detectable levels of targeted antimicrobial drugs tested with standard solutions were 0.025∼1.0 ppm for 7 beta-lactams, 0.5∼1.0 ppm for 4 aminoglycosides, 0.05∼0.5 ppm for 5 macrolides, 0.05∼0.25 ppm for 3 tetracyclines and 0.25&1.0 ppm for 6 sulfonamides. In comparison of FPT and Charm II test, the results of FPT were not accord with those of Charm II test having the group specificity of seven families of antimicrobial drugs in meat samples except some families like tetracyclines.

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Manufacture of Antimicrobial Paper for Food Products (식품포장용 항균지 제조)

  • 이명구;이상명;오덕환
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.32 no.1
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    • pp.65-71
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    • 2000
  • Chitosan oligosaccharide, chitosan polysaccharide and monolaurin were used to impart the antimicrobial properties onto paper for food products . Staphylococcus aureus (ATCC 25923) was used as test microorganism and shake flask test was carried out in order to examine the antimicrobial activity of each sample. Chitosan oligosaccaride showed the greatest antimicrobial effect among them and gave the promising result even at the concentration of 0.005%. Its antimicrobial power was enhanced when it is used with nomolaurin together. Chitosan oligosaccharide improved the tensile strength of antimicrobial paper.

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Antibiosis Evaluation of Antimicrobial Mortar by Artificial Accelerating Test for Biochemical Corrosion (생화학적 부식 인공촉진시험에 의한 항균모르타르의 항균성능 평가)

  • Koo, Kyung-Mo;Shin, Kwan-Soo;Roh, Kyung-Min;Lee, Eui-Bae;Kim, Gyu-Yong;Kim, Moo-Han
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2007.11a
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    • pp.33-36
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    • 2007
  • In this study, an antibiosis of antimicrobial mortar developed to reduce biochemical corrosion of sewage facilities concrete was evaluated. First, artificial acceleration test reflecting similarly biochemical corrosion of mortar was developed. Antimicrobial mortar specimen were experimented in this test and antibiosis of it was evaluated by SEM and EDX. As a results of the study, sulfur-oxidizing bacteria on the plain specimen were survived for 20 weeks in this test. But sulfur-oxidizing bacteria on antimicrobial specimen were survived less than the plain, and sulfur-oxidizing bacteria were externally distorted and destroyed. So the antibiosis of an antimicrobial mortar was verified by it.

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Antimicrobial Activity and Safety Test of Natural extract including Phellodendro namurense, Eucommia ulmides Oliv extracts (황벽나무, 두충나무 추출물 등을 포함한 천연 추출물의 항균력 및 안전성)

  • Noh, Daeyoung;Joe, Suehyeon;Yang, Hyunguk;Han, Donggyun;Kim, Jinhong;Kim, Donguk
    • Korean Chemical Engineering Research
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    • v.54 no.6
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    • pp.762-766
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    • 2016
  • In this study, natural extract including Phellodendro namurense, Eucommia ulmides Oliv and Prunus padus were tested for antimicrobial activity and safety. Antimicrobial activity was measured by using opportunistic microbes such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans. As safety test, cell viability test, single dose oral toxicity test, single dose inhalation toxicity test, repeat dose inhalation toxicity test and eye irritation test were done. From antimicrobial test, natural extract showed execellent antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Escherichia coli. From 5 kinds of safety tests, toxicity was not observed. From experimental results, natural extract including Phellodendro namurense, Eucommia ulmides Oliv and Prunus padus showed superb safety and antimicrobial effect.

ANTIMICROBIAL SUSCEPTIBILITY TEST ON ORAL FLORA FROM DIFFERENT SAMPLING SITES IN CHILDREN (소아에서 구강내 정상세균총에 대한 채취부위별 항균제 감수성 차이에 관한 연구)

  • Shin, Sang-Hun;Kim, Boo-Kyoung;Song, Jung-Ho;Park, Sung-Hwan;Chung, In-Kyo
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.26 no.1
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    • pp.40-44
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    • 2000
  • The most proper antibiotic must be selected after antimicrobial susceptibility test. If difference in antimicrobial susceptibility was significant between oral sampling sites, it is rationale to use the most susceptible antibiotic agent respond to dental procedure and object of treatment. This study examined sampling site variation from saliva, supragingival plaque and subgingival plaque of 16 children's oral microbes. The cultured bacterial isolates, which were Streptococcus viridans and Neisseria, were examined for 10 antimicribial drugs with the Bauer-Kirby agar disk diffusion method. The used drugs were Penicillin, Ampicillin, Oxacillin, Cephalothin, Imipenem, Gentamicin, Erythromycin, Vancomycin, Ciprofloxacin, Clindamycin. There was no significant difference between three sampling sites for antimicrobial susceptibility test of S. viridans and Neisseria and the sequence of susceptibility was agreed among them. In conclusion, it was suggested that antimicrobial susceptibility test from saliva, supragingival plaque and subgingival plaque of children have no significant sampling site variation.

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Manufacture of Antimicrobial Paper Using Food Preservative (식품보존제를 이용한 항균지 제조)

  • 이진호;이장호;박종문
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.33 no.2
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    • pp.81-86
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    • 2001
  • The functions of food packaging are not only prevention from physical damage and loss during carrying and transportation, but also extension of shelf-life by adding antimicrobial substrate in packaging materials. Consumption of active packaging is gradually increasing. With different dosage of potassium sorbate(P.S.), the food preservative agent, antimicrobial papers were made by internal and external application of starch. The antimicrobial action of the paper was analyzed by the halo test and the shake flask method. The mechanical properties and strength were also measured. Antimicrobial papers adding P.S. showed higher values in tensile index than adding starch. The antimicrobial paper using starch showed similar microbe decreasing rate as that using P.S. Though microbe decreasing rate was 21.9%, it showed possibility to make antimicrobial paper using food preservative.

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Antibiotic effects of Medicinals resolving dampness with aroma on vaginal microorganisms (방향화습약(芳香化濕藥)이 질내(膣內) 미생물(微生物)에 미치는 영향)

  • Lee, Chang-Hoon;Cho, Jung-Hoon;Jang, Jun-Bock;Lee, Kyung-Sub;Kim, Kyung-Sook
    • The Journal of Korean Obstetrics and Gynecology
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    • v.19 no.4
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    • pp.33-46
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    • 2006
  • Purpose : This study was conducted to investigate effects of 7 herbs among medicinals resolving dampness with aroma on vaginal microorganisms. Methods : Staphylococcus aureus, Methicillin- resistant Staphylococcus aureus, Candida albicans and Gardnerella vaginalis were used for vaginal pathogenic microorganisms. Lactobacillus gasseri, Streptococcus spp. and Escherichia coli HB101 were used for vaginal flora. Medicinals resolving dampness with aroma, Pogostemonis Herba, Amomi Cardamomi Fructus, Amomi Semen, Atractylodis Rhizoma, Ammomi Tsao-ko Fructus, Alpiniae Katsumadaii Semen and Magnoliae Cortex were used in this study. In vitro antimicrobial activities were observed by optical density and colony test. Results : The optical density showed that Alpiniae Katsumadaii Semen and Magnoliae Cortex among medicinals resolving dampness with aroma had antimicrobial effects on pathogenic vaginal microorganisms (Methicillin-resistant Staphylococcus aureus). Pogostemonis Herba, Ammomi Tsao-ko Fructus had antimicrobial effects on Gardnerella vaginalis. The colony test showed that Alpiniae Katsumadaii Semen, Magnoliae Cortex among medicinals resolving dampness with aroma had antimicrobial effects on Methicillin-resistant Staphylococcus aureus. Pogostemonis Herbs, Alpiniae Katsumadaii Semen had antimicrobial effects aganist Gardnerella vaginalis. The optical density showed that Magnoliae Cortex among medicinals resolving dampness with aroma had antimicrobial effect on normal vaginal microorganisms (Streptococcus spp.). The colony test showed that medicinals resolving dampness with aroma had no antimicrobial effect on normal vaginal microorganisms. Conclusion : From this study, we could suggest that Pogostemonis Herba, Ammomi Tsao-ko Fructus, Alpiniae Katsumadaii Semen, Magnoliae Cortex of medicinals resolving dampness with aroma are available to antimicrobial agent of pathogenic vaginal microorganisms in vitro.

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Antimicrobial effects of Astringent medicinals against vaginal microbe (수삽약(收澁藥)이 질내(膣內) 징생물(徵生物)에 미치는 영향(影響))

  • Lee, Chang-Hoon;Cho, Jung-Hoon;Jang, Jun-Bock;Lee, Kyung-Sub;Yoon, Young-Jin
    • The Journal of Korean Obstetrics and Gynecology
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    • v.19 no.2
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    • pp.127-140
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    • 2006
  • Purpose : This study was conducted to investigate the antimicrobial effects of Astringent medicinals against vaginal microbe. Methods : Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Candida albicans and Gardnerella vaginalis were used for vaginitis-induced microbe. Lactobacillus gasseri, Streptococcus spp. and Escherichia coli HB101 were used for normal vaginal florae. And Astringent medicinals (Rubi Fructus, Tritici Immatri Semen, Corni Fructus, Nelumbinis Semen, Mume Fructus, Schizandrae Fructus, Ailanthi Radicis Cortex, Galla Rhois, Myristicae Semen, Terminariae Fructus, Rosae laevigatae Fructus, Ephedrae Radix) were used in this study. Antimicrobial activities were tested by optical density and colony test in vitro. And then wee valuated the antimicrobial effects in comparison with optical density and colony test. Results : The optical density and colony test showed that Terminariae Fructus and Galla Rhois among Astringent medicinals had the antimicrobial effects. Teminariae Fructus had the antimicrobial susceptibility and selective toxicity against Candida albicans and Gardnerella vaginalis of vaginitis-induced microbe. Galla Rhois had the antimicrobial susceptibility and selective toxicity against Staphylococus aureus, Methicillin-resistant Staphylococus aureus, Candida albicans and Gardnerella vaginalis of vaginitis-induced microbe. Conclusion : According to these results, we can suggest that Terminariae Fructus and Galla Rhois among Astringent medicinals be available to the antimicrobial agent of vaginitis-induced microbe in vitro.

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Antimicrobial Activity and Safety Test of Mixed Plant Extracts Including Phellodendron Amurense and Eucommia Ulmides Oliv (황벽나무, 두충나무 등을 포함하는 복합수목추출물의 항균활성 및 안전성 시험)

  • Kim, Hyunwoo;Shin, Hyejin;Hwang, Danbi;Lee, Jieun;Bak, Manchun;Kim, Jinhong;Kim, Donguk
    • Korean Chemical Engineering Research
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    • v.51 no.5
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    • pp.536-539
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    • 2013
  • In this study, mixed plant extracts including Phellodendro namurense and Eucommia ulmides Oliv were tested for antimicrobial activity and safety. Antimicrobial activity was measured by disc diffusion method using normal skin flora and opportunistic microbes such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans. As safety test, single dose oral toxicity test, single dose inhalation toxicity test and repeat dose inhalation toxicity test were done. From antimicrobial test, plant extracts showed significant antimicrobial activity Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, however, did not indicate any antibacterial effect on Escherichia coli. From 3 kinds of safety tests, toxicity of mixed plant extracts was not observed. From experimental results, mixed plant extracts including Phellodendro namurense and Eucommia ulmides Oliv showed good potential for natural antimicrobial agent.

A Study on the Improvement of Antimicrobial Activity and Crease Resistance of Korean Traditional Hansan Ramie Fabrics (한국 전통한산모시의 항균성 및 방추도 향상에 관한 연구)

  • Kim, Ho Jung;Choi, Jae Hyeok;Kim, Han Do;Park, Cha Cheol
    • Textile Coloration and Finishing
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    • v.6 no.4
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    • pp.1-8
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    • 1994
  • To improve the antimicrobial activity and crease resistance of Korean traditional Hansan ramie fabrics, partially saponified polyethylene(PPS) was used as a softening water repellent agent and 3-(trimethoxysilyl)propyl dimethyl octadecyl ammonium chloride(QAC) as a antimicrobial agent. The antimicrobial activity was effective by treatments of PPS, QAC and PPS/QAC for shake flask test. For the halo test, PPS/QAC showed good antimicrobial activity. It was found that the desirable composition of H$_{2}$O/PPS/QAC is 98/1/1 for improving the antimicrobial activity and crease resistance of the fabrics.

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