• Journal of the Korean Wood Science and Technology
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• v.33 no.2 s.130
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• pp.72-77
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• 2005

Streptococcus mutans is known as a strong cariogenic bacterium and produces dental caries. This study was carried out to develop anticariogenic agents from Callistemon citrinus. Crude extracts and thier various solvent fractions of the plant extracts were tested for the antibacterial activity against S. mutans. The anticariogenic activity was evaluated with by paper disc diffusion method and minimal inhibition concentration (MIC). The extract from the fruit and its hexane fraction showed a strong inhibitory effect on the growth of S. mutans and the MICs were 31.2 and $3.9{\mu}g/m{\ell}$, respectively.

• Korean Journal of Pharmacognosy
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• v.47 no.1
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• pp.43-48
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• 2016

The aim of the study was performed to examine the anticariogenic potential of purified bee venom (Apis mellifera L., PBV) collected using bee venom collector from cariogenic bacteria, Streptococcus mutans, Streptococcus sanguis, Porphyromonas gingivalis, and Fusobacterium nucleatum. The anticariogenic effect of purified bee venom was evaluated by agar well diffusion method, minimum inhibitory concentraion (MIC), minimum bactericidal concentration (MBC), and postantibiotic effect (PAE). The human lower gingiva epithelial cell cytotoxicity of purified bee venom was also evaluated. Purified bee venom exhibited significant inhibition of bacterial growth of S. mutans, S. sanguis, P. gingivalis, and F. nucleatum with MIC value of 0.68, 0.85, 3.49, and $2.79{\mu}g/ml$, respectively. The MBC value of purified bee venom against S. mutans, S. sanguis, P. gingivalis, and F. nucleatum was 1.34, 1.67, 8.5, and $6.8{\mu}g/ml$. Furthermore, the results of PAE values against S. mutans, S. sanguis, P. gingivalis, and F. nucleatum showed the bacterial effect with 3.3, 3.45, 2.0, and 2.0. The concentration below 1 mg/ml of purified bee venom had no cytotoxicity in the human lower gingiva epithelial cell. These results suggested that purified bee venom have great potenial as anticariogenic agents.

• Journal of the korean academy of Pediatric Dentistry
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• v.40 no.4
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• pp.247-252
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• 2013

Pit and fissure sealant prevents biofilm accumulation, plays a role in forming a barrier to acidic substance made by the bacteria. The Surface reaction-type pre-reacted glass ionomerI(S-PRG) filler was developed in 1999. S-PRG filler releases fluoride continuously and does not decompose under wet conditions. The aim of this study was to test the microleakage and anticariogenic effect to adjacent enamel of S-PRG filler-containing pit and fissure sealant. Sound premolars and molars were used in this study. A S-PRG filler-containing pit and fissure sealant, Beautisealant$^{(R)}$(Shofu, Japan) was used for this experiment, the composite resin sealant Concise$^{(R)}$(3M ESPE, USA) was used as control. For the microleakage test, all teeth surface were double coated with finger nail varnish, with the exception of a 1.0 mm window around the restoration margins. The teeth were immersed in 2% methylene blue solution for 24 hours and then rinsed in tap water. For the anticariogenic effect evaluation, all tooth were immersed in artificial carious solution for 9 days and rinsed with tap water. Each tooth was embedded in orthodontic acrylic rein and subsequently sectioned longitudinally in a bucco-lingual direction with a low-speed diamond saw. The cut sections were examined using a stereomicroscope. Differences in microleakage between the two groups were not different significantly. But the S-PRG filler-containing pit and fissure sealant showed higher anticariogenic effect than that of flowable resin sealant.

• Journal of the korean academy of Pediatric Dentistry
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• v.25 no.4
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• pp.849-857
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• 1998

The purpose of this study was to investigate whether fluoride-releasing pit and fissure sealant was more effective in preventing caries than conventional non-fluoride-releasing sealant. Specimens 8mm in diameter were made from sound bovine enamel. Fluoride-releasing pit and fissure sealant(Helioseal F, Vivadent, Schaan, Liechtenstein) and non-fluoride-releasing sealant(Helioseal, Vivadent, Schaan, Liechtenstein) were applied to the specimens and artificial caries was induced. Microhardness and the depth of the carious lesion was measured. The following results were obtained: 1. In group 2, sealed with fluoride-releasing sealant, there was a 58.4% decrease in microhardness. This was significantly less than the 84.4% decrease observed in group 1, sealed with non-fluoride-releasing sealant(p<0.01). 2. The average depth of the artificial carious lesion in group 2 was $30.1{\pm}9.8{\mu}m$. In group 1, sealed with non-fluoride-releasing sealant, the lesion was significantly deeper with an average depth of $58.5{\pm}4.9{\mu}m$(p<0.01). 3. Fluoride-releasing pit and fissure sealant was more anticariogenic compared to non-fluoride-releasing sealant.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.462-466
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• 2023

After treating spent coffee grounds with alkali, extracts were prepared by using Viscozyme and Alcalase, respectively. Treatment of spent coffee grounds with alkali and enzymes increased the content of phenolic compounds in the extracts, thus possessing the good scavenging activities on free and cation radicals. In particular, the extract obtained by continuous treatment with alkali and Alcalase on spent coffee grounds had the best content of phenolic compounds and antioxidant activity, and inhibited the growth of Streptococcus mutans in proportion to the concentration. In conclusion, the Alcalase-enzymatic hydrolysate of alkali-treated spent coffee grounds showed excellent antioxidant and anticariogenic effects.

• Journal of the korean academy of Pediatric Dentistry
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• v.24 no.1
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• pp.148-172
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• 1997

Sixty human premolar teeth were used for this in vitro study. After each tooth was sectioned mesiodistally, one half was used for the experimental group and the other half for the control. Three groups were made for each fluoride applying method and twenthy teeth were assigned to each group. Ten teeth were used for evaluating total fluoride amount and the other ten were used for firmly-bound fluoride. Fluorshield was used for fluoride-releasing sealant and 1.23% APF, 0.05% NaF were used for topical application fluorides. Each tooth was cleaned with a tooth brush using nonfluoride containing pumice before the experiment. In the sealant group, fluoroshield was applied to the enamel surface without etching procedure and stored in $37^{\circ}C$ saline for 30 days. After 30 days, sealant was removed with explorer without scratching the enamel surface and washed with distilled water and dried. In the APF group, each tooth was immersed in 1.23% APF for 30 min then washed and dried in the same manner. In the NaF group, each tooth was immersed in 0.05% NaF for 24 hours then washed and dried as described above. After each fluoride regimen was applied, ten teeth were randomly selected from each group and immersed in 1M KOH solution for 24 hours to remove loosely-bound fluoride possibly deposited by the three different fluorides applied. In each group, total fluoride amount deposited and the amount of enamel removed by acid biopsy were calculated. After loosely-bound fluoride was removed, firmly-bound fluoride deposited and the amount of enamel removed by acid biopsy were also calculated. Total fluoride amount deposition was significantly increased in the APF and NaF groups, but not in the sealant group. Amount of enamel removed by acid-biopsy was also significantly diminished in the APF and NaF groups, but not in the sealant groups. After loosely-bound fluoride was removed from each groups, no statistical difference was found in the amount of firmly-bound fluoride in any groups. Also no effect of firmly-bound fluoride on enamel dissolution was shown in any groups after loosely-bound fluoride was removed from each group. In conclusion, topical application method of APF or NaF is more effective than fluoride-releasing sealant application to make $CaF_2$ coating on enamel surface and $CaF_2$ coating is the main source for anticariogenic effect of fluoride. However, longterm anticariogenic effect of fluoride-releasing sealant should be further evaluated.

• Journal of the korean academy of Pediatric Dentistry
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• v.25 no.1
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• pp.62-75
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• 1998

The main purpose of this study was to compare the anticariogenecity of glass ionomer restorative material polymerized by argon laser versus visible light, The results from the present study can be summarized as follows; 1. Under the polarized light microscope, the specimens of laser-cured group showed the shallower lesion body than that of visible-light cured group, both in the stage of lesion initiation and progression. 2. Glass ionomer material cured by visible light showed shallower body of lesion than that of composite resin cured by argon laser at the stage of lesion progression(p<0.05). It was suggested fluoride released from the glass ionomer might have the additive anticariogenic effect. 3. Statistical difference between groups on depth of lesion body was evident after lesion progression (p<0.05). It was suggested that anticariogenic effect by argon laser was more effective at the stage of lesion progression than the lesion initiation. 4. The increment of lesion body during progression was highest in group IV (p<0.05). 5. Based upon the above-mentioned results of this study, it can be concluded that the advantage of anticariogenic effect and short curing time of argon laser in glass ionomer polymerization should be considered in children and adolescents whose caries activity is relatively higher.

• Journal of the korean academy of Pediatric Dentistry
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• v.29 no.1
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• pp.1-10
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• 2002

The purpose of this study was to evaluate the fluoride release and anticariogenic effect of two compomers which is known to have been developed to improve the weak properties of glass ionomer cement and composite resin. Z-100 as composite resin(Group I), Dyract AP(Group II) and F-2000(Group III) as compomer, and Fuji II LC as glass ionomer cement(Group IV) were used as test materials and evaluations were peformed by pH/ISE meter far analyses of fluoride and polarizing microscope for analyses of anticariogenicity. The results can be summarized as follows: 1. The amount of fluoride release in compoite resin and compomer groups showed general pattern of decline during test period. Z-100 showed no fluoride release during test period. Fuji II LC showed the highest fluoride release among test groups and then F-2000 were followed. 2. The least resistance to dimineralization was observed microscopically in Z-100 group which has no fluoride in it. The best resistance to dimineralization was observed microscopically in Fuji II LC group and then compomer groups were followed. 3. Significant difference in lesion area was found between Fuji II LC group and another groups. Significant difference in lesion area was found between compomer groups and Z-100 group. No significant difference in lesion area was found between Dyract AP group and F-2000 cup. 4. Two compomers showed continual fluoride release and anticariogenic effect around filling materials. therefore, compomer was evaluated very attractive restorative material in pediatric dentistry.

• Journal of Environmental Health Sciences
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• v.33 no.2 s.95
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• pp.137-144
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• 2007

Yam, Prunella was stepwise extracted with hexane, chloroform, ethyl acetate, butanol, and water. Anti-microbial activity of each extract was investigated. Hexane extract was tested for anti-microbial effect on Streptocaccus mutans, one of causative factor of dental caries. Methanol extracts of 7 plants were investigated to anti-microbial effects on S. mutans KCTC 5316, P. gingivalis KCTC 5352, S. aureus KCTC 1927 by means of agar diffusion method. Methanol extract of Yam and Prunella revealed anti-microbial activity against S. mutans, P. gingivalis, and S. aureus. Also, hexane fraction of Yam revealed anti-microbial activity against S. mutans. In sequence of hexane, chloroform, ethylacetate, butanol fraction by Prunelia acted as potent anti-microbial agent on P. gingivalis. The measured MIC of hexane fraction of Yam and Prunella on S. mutans KCTC 5316 strain was 0.25 mg/ml and 0.5 mg/ml and the MIC of hexane fraction of Prunella on S. aureus was 0.5 mg/ml. The hexane fraction of Yam and Prunella suppressed viable ceil counts(VCC) of S. mutans, especially after 24 hrs. The Prunella hexane fraction suppressed VCC of S. aureus, after 12 and 24 hrs. Tested concentrations were 0.1, 0.25 and 0.5 mg/ml. the results were compared with control (0 mg/ml). The pH of S. mutans media and GTase activity were determined to evaluate the anticariogenic activity of Yam, Prunella hexane fraction. The pH were increased from 5.6 to 7.0-7.2 in concentration of 2.0 mg/ml. Yam hexane extraction revealed 35% inhibition to GTase activity and Punella inhibited 25% of GTase. These results suggest that the hexane extracts of Yam and prunella have Antibacterial activities against S. mutans, P. gingivalis, S. aureus and have preventive effect on dental caries.

• Journal of dental hygiene science
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• v.13 no.4
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• pp.461-470
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• 2013

In order to examine the anticariogenic effect after fluoride varnish was applied to sound enamel and artificial caries enamel, anterior teeth of healthy cattle were used and divided into four groups such as group 1 (sound enamel), group 2 (application of fluoride varnish to sound enamel), group 3 (artificial caries enamel) and group 4 (application of fluoride varnish to artificial caries enamel). Remineralization on the surface of enamel and changes of crystalline structure after demineralization were observed by using a field emission scanning electron microscope (FE-SEM). Quantitative analysis of Ca and P was measured by using the energy dispersive X-ray spectrophotometer (EDS). The following conclusions were obtained: 1)Surface pattern of enamel was the roughest in group 3 due to the defects caused by porosity and microcracks. Group 4, group 1 and group 2 were followed in order; 2) It was found that pattern of crystalline structures in a group of application of fluoride varnish and a group of no application showed bigger change in artificial caries enamel than that in sound enamel. In other words, groups 4 and 1 showed a similar pattern; 3) The contents of Ca and P were higher in groups of application of fluoride varnish (group 2 and group 4) than in groups of no application of fluoride varnish (group 1 and group 3). Taken results of this study together, in the case of application of fluoride varnish, crystalline structure was changed by remineralization even in the sound enamel. In particular, porous structures showed a smooth and uniform pattern due to the recalcification in the artificial caries enamel. In addition, according to results of EDS analysis, the contents of Ca and P were increased and it had great anticariogenic effects which inhibit decalcification of sound enamel and artificial caries enamel.