• 한국조리학회지
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• 제17권5호
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• pp.241-252
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• 2011

본 연구는 천일염, 구운 소금, 1회 구운 죽염과 9회 구운 죽염으로 만든 된장에서 항암효과를 평가하기 위하여 수행되었다. MTT 분석에서 천일염된장(S-D), 구운 소금된장(R-D), 1회 구운 죽염 된장(B1-D) 및 9회 구운죽염 된장(B9-D)을 0.05%와 0.1%를 첨가하였을 때 인체위암세포인 AGS의 증식에 대하여 상당한 저해효과(64-87%)를 나타내었다. Sarcoma-180 세포를 주사한 Balb/c 마우스 가운데 4종류의 된장을 투여한 쥐의 체중, 간장, 비장, 간장과 심장의 무게는 된장을 투여하지 않은 군보다 무거웠다. 또한 4가지 종류의 된장은 쥐의 종양 형성을 크게 저해하였으며, 특히 R-D와 B1-D는 1.0mg/kg 투여함으로써 97%의 종양세포의 성장억제를 나타내었다. 자연살해세포의 활성은 4종류의 된장을 투여한 쥐에서 상당히 높았으며 R-D를 투여한 쥐에서는 88.2%의 강한 활성을 나타내었다. 4종류의 된장을 투여한 쥐에서 glutathione S-transferase(GST)는 된장을 투여하지 않은 쥐보다 높았으며, 특히 GST 활성은 B1-D를 투여한 군에서 가장 높게 나타났다. 이러한 결과들은 여러 가지 종류의 소금이 된장의 항암활성에 미치는 영향에 차이가 있는 것으로 사료된다.

• 대한약침학회지
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• 제5권1호
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• pp.43-52
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• 2002

Objectives: This study was purposed to investigate the sarcoma-180 anticancer effects of Herbal acupuncture with Juglandis Semen(JsD) in mice. Methods: The Juglandis Semen Herbal-acupuncture was injected on Chung-wan(CV12) of mice with S-180 cancer cell line. Results: The results obtained were summarized as follows; 1. Median survival time of S-180 cancer cell treated with Juglandis Semen Herbal-Acupuncture was not significant.(p < 0.05) 2. Natural killer cell activity was insignificant for S-180 cell treated with Juglandis Semen Herbal-Acupuncture Herbal acupuncture. (P < 0.05) 3. Interleukin-2 productivity of S-180 cell treated with Juglandis Semen Herbal-Acupuncture was not significant.(P < 0.05) Conclusions: According to the results, we can conclude Herbal-acupuncture with Juglandis Semen caused no effects in S-180 cancer cell.

• 한국의류학회지
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• 제24권8호
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• pp.1146-1154
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• 2000

The purpose of this study was to investigate the antimicrobial activity, antimutagenic and anticancer effects and dyeing properties of the fermented indigo extract. The methanol extract of fermented indigo showed a strong inhibition effect on Trich. mentagrophytes and antimutagenic activities against aflatoxin B$_1$(AFB$_1$) in the Ames test using Salmonella typhimurium TA 100. We also found in vitro anticancer effects of the methanol extract of fermented indigo and it was evaluated by using Clone M-3 mouse melanoma cells and A431 human epidermoid carcinoma cells and exerted little cytotoxity against 3T3-L1 embryo fibroblast cell. In the relationship between the K/S values of cotton and silk fabrics dyed with fermented indigo and dyeing repeating times, the K/S values became higher as the repeating times were increased. The K/S values were high when the fabrics were dyed at low temperture. The K/S values of cotton fabrics were higher than those of silk fabrics. Changes of surface color of silk fabrics were higher than those of cotton fabrics after water fastness test, laundering, irradiation and treatment of acidic and alkaline perspiration.

• 한국발생생물학회지:발생과생식
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• 제21권2호
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• pp.139-150
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• 2017

Metformin is the most commonly prescribed anti-diabetic drug with relatively minor side effect. Substantial evidence has suggested that metformin is associated with decreased cancer risk and anticancer activity against diverse cancer cells. The tyrosine kinase inhibitor imatinib has shown powerful activity for treatment of chronic myeloid leukemia and also induces growth arrest and apoptosis in colorectal cancer cells. In this study, we tested the combination of imatinib and metformin against HCT15 colorectal cancer cells for effects on cell viability, cell cycle and autophagy. Our data show that metformin synergistically enhances the imatinib cytotoxicity in HCT15 cells as indicated by combination and drug reduction indices. We also demonstrate that the combination causes synergistic down-regulation of pERK, cell cycle arrest in S and $G_2/M$ phases via reduction of cyclin B1 level. Moreover, the combination resulted in autophagy induction as revealed by increased acidic vesicular organelles and cleaved form of LC3-II. Inhibition of autophagic process by chloroquine led to decreased cell viability, suggesting that induction of autophagy seems to play a cell protective role that may act against anticancer effects. In conclusion, our present data suggest that metformin in combination with imatinib might be a promising therapeutic option in colorectal cancer.

• Food Science and Biotechnology
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• 제18권1호
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• pp.1-11
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• 2009

Carotenoids being most important pigments among those occurring in nature, have received increased interest owing to their beneficial effects on human health. An effort is made to review marine carotenoids as important bioactive compounds with reference to their presence, chemical, and biofunctional benefits they afford. The potential beneficial effects of marine carotenoids were particularly focused on astaxanthin and fucoxanthin, major marine carotenoids found in marine animals and aquatic plants, respectively. Both carotenoids show strong antioxidant activity which is attributed to quenching singlet oxygen and scavenging free radicals. The potential role of the carotenoids as dietary antioxidants has been suggested as being one of the main mechanism by which they afford their beneficial health effects such as anticancer activity and anti-inflammatory effect. Only recently, antiobesity effect and antidiabetic effect have been noted as specific and novel bio-functions of fucoxanthin. Nutrigenomic study reveals that fucoxanthin induces uncoupling protein 1 (UCP1) expression in white adipose tissue (WAT) mitochondria to lead to oxidation of fatty acids and heat production in WAT. Fucoxanthin improves insulin resistance and decreases blood glucose level, at least in part, through the down-regulation of tumor necrosis factor $\alpha$ ($TNF{\alpha}$) in WAT of animals.

• 대한한의진단학회지
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• 제22권1호
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• pp.11-18
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• 2018

Objectives The incidence of degenerative diseases is increasing as society ages, and this increased the economic burden on society. Oxidative stress was also under suspicion as one of the causes. Recent studies have found that Curcuma longa Rhizoma had antioxidant, anti-inflammatory, antimutagenic, and anticancer effects. Through this study, we figure out trends in Curcuma longa Rhizoma research and evaluate the therapeutic potential of Curcuma longa Rhizoma for dementia. Methods The search was made using domestic and international internet search sites. "Naver academic," "Google scholar," "Korea institute of oriental medicine (KIOM OASIS)," "Korean traditional knowledge portal (KTKP)," "Library of Congress," "Research information sharing service (RISS)," "National Digital Science Links (NDSL)," and "Pub med" are used to search. The searched for words were "turmeric," "Curcuma longa Rhizoma," "antioxidant," and "nerve regeneration." Results It could be seen that a lot of research done on Curcuma longa recently. In previous studies, the most common research disease was cancer. In previous studies, the most common effects of Curcuma longa was antioxidant and anticancer. Curcumin, Vitamin B1, and various essential oils were the functional components of Curcuma longa Rhizoma. Curcuma longa Rhizoma performs DPPH radical scavenging activity and ABTS radical cation decolorization activity; it inhibits NO and iNOS. Conclusion The destruction of nerve cells due to oxidative stress is a cause of dementia. Curcuma longa Rhizoma have antioxidant effects. Therefore, we believed that it is an effective treatment for dementia. Various studies will be made on Curcuma longa Rhizoma.

• 통합자연과학논문집
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• 제5권1호
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• pp.46-52
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• 2012

Mulberry extracts can be incorporated into skin-whitening products. The compound attributed to lighten the skin is arbutin, a form of hydroquinone that inhibits melanin release by suppressing the tyrosinase enzyme. For the cosmetic applications, the physiological effects of mulberry (Morus alba) extracts were investigated. The water soluble fraction of mulberry contains higher amount of protein (16.28~4.47%) in contrast to fat (1.55~1.41%). In addition, the fraction abundantly contains succinic acid (972.4-275.8 mg/g) and phosphoric acid (1,628.4-121.9 mg/g) in different parts of mulberry. The free radical scavenging ability in water soluble fraction was found to display remarkable effects in comparison with methanol and ethyl acetate fraction. The ethyl acetate-soluble of root and leaf showed remarkable tyrosinase inhibition activity by IC 50 (${\mu}g/ml$). The anticancer activity of methanol fraction obtained from mulberry using human cancer cell lines showed growth inhibition effect (270.14 mg/ml in Calu-6 cells, 295.29 mg/ml in HCT-116, and 332.29 mg/ml in MCF-7 cells, respectively). Based on the results, Morus alba extracts include cosmetic ingredients with antioxidizing and whitening properties.

• 대한수의학회지
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• 제61권3호
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• pp.22.1-22.7
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• 2021

Fenbendazole (FBZ) is a commonly used anthelmintics in veterinary medicine that has recently been found to have anticancer effects in humans. On the other hand, few studies have examined the anti-inflammatory effects of FBZ, and its mechanism is unknown. In this study, mouse bone marrow cells (BMs) were treated with lipopolysaccharide (LPS), a representative inflammation-inducing substance, to generate a situation similar to osteomyelitis in vitro. The effect of FBZ on inflammatory BMs was examined by measuring the metabolic activity, surface marker expression, cell nuclear morphology, and mitochondrial membrane potential (MMP) of BMs. FBZ decreased the metabolic activity and MMP of LPS-treated BMs. Annexin V-fluorescein isothiocyanate/propidium iodide staining and Hoechst 33342 staining showed that FBZ reduced the number of viable cells and induced the cell death of inflammatory BMs. In addition, FBZ reduced the proportion of granulocytes more than B lymphocytes in LPS-treated BMs. Overall, FBZ induces cell death by destabilizing the MMP of LPS-induced inflammatory BMs. In addition to anthelmintic and anticancer agent, FBZ can play a role as an anti-inflammatory agent.

• 한국해양바이오학회지
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• 제14권2호
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• pp.93-101
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• 2022

Hizikia fusiforme, a type of brown algae, is widely used in Asian cuisine. It has been reported to have various pharmacological effects. In this study, the effects of the ethanol extract from H. fusiforme (EAHF) on the proliferation of human colon carcinoma cells were investigated. The effect on the survival of human hepatocarcinoma and colon carcinoma cells was examined, and results revealed that the anti-proliferative effects of EAHF were higher in colon carcinoma cells than in hepatocarcinoma cells. The inhibition of proliferation of HT29 colon carcinoma cells by EAHF treatment was closely related to the induction of apoptosis. EAHF treatment also increased caspase activity and poly(ADP-ribose) polymerase degradation, induced mitochondrial dysfunction, altered Bcl-2 family protein expression, and increased the rate of cytochrome c released from the mitochondria into the cytoplasm. Furthermore, the production of reactive oxygen species (ROS) was markedly stimulated by EAHF treatment, and when ROS production was blocked, EAHF-induced cytotoxicity was significantly attenuated. These results indicate that the anticancer activity of EAHF in HT29 colon carcinoma cells was induced by ROS-dependent mitochondrial impairment. While EAHF exhibited potent anticancer activity in colon carcinoma cells in this study, further studies on the active components of EAHF and their efficacy should be performed.

• Journal of Preventive Medicine and Public Health
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• 제20권1호
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• pp.1-9
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• 1987

항암제와 세포독성과의 관계를 알아보고자 인혈배양 임파구에서 SCE빈도, 세포분열지수 및 세포분열주기변동을 조사한 결과는 다음과 같다. 1) 항암제 농도증가에 따라 SCE빈도는 대조군에 비해 유의하게 증가되었으며 (P<0.01), methotrexate에서는 유의성이 인정되지 않았다(P>0.05). 2) 세포분열지수는 cyclophosphamide를 제외한 공시 항암제에 대하여 현저하게 감소되었다. 3) 항암제의 농도증가에 따라 세포분열주기는 현저하게 지연되었으며 고농도에서는 현저한 세포분열 억제로 정상적인 세포분열이라 할 수 없는 강한 세포독성효과를 보였다. 이 결과는 알킬계 약제가 다른 항암제에 비해 강한 SCE 유발제이며 SCE유발과 세포분열주기 지연과는 깊은 상관성이 인정되지만 methotrexote경우에서는 그 상관성을 인정할 수 없었다. 이는 SCE유발과 세포분열주기지연이 서로 다른 기전에 의해서도 나타남을 제시해주고 있다.