• Title/Summary/Keyword: Antibody titers

Search Result 425, Processing Time 0.027 seconds

Immunological significance of recombinant VP2 and VP3 proteins of aquabirnavirus in olive flounder, Paralichthys olivaceus

  • Kim, Hyoung Jun;Kim, Ki Hong;Park, Jeong Su;Lee, Haeng Lim;Kwon, Hyuk Chu;Kwon, Se Ryun
    • Journal of fish pathology
    • /
    • v.28 no.2
    • /
    • pp.93-98
    • /
    • 2015
  • Full-length recombinant VP2 and VP3 proteins of aquabirnavirus isolated from olive flounder were expressed successfully in E. coli expression system. After rats were immunized with these proteins, antisera were used for in vitro and in vivo neutralization test. In in vitro test, VP2 antibody titers were higher than that of VP3. In in vivo assays, fish challenged with aquabirnavirus neutralized with VP2 antibody survived longer than other fish.

Server on the Distribution of Bovine Toxoplasma Antubodies by Latex Agglutination Test in Gyeongnam District (경남지역에서의 Latex응집반응을 이용한 소 톡소플라즈마 항체분포 조사)

  • 이병훈;류중운;황보원;변유성;조광제
    • Korean Journal of Veterinary Service
    • /
    • v.18 no.3
    • /
    • pp.36-41
    • /
    • 1995
  • This study was conducted to determine the serum antibodies against toxoplasma in bovine from gyeongnam district by latex agglutination(LA) test. LA test was carried out with Toxo-MT kit(Eikon chemical co). The result obtained were summerized as follow: 1. Positive rates of toxoplasma antibodies in 1,488 bovine sera was 5.0%(75 cases) by LA test. 2. The toxoplasma antibody detection rates against 823 korean cattle and 865 dairy cattle were 1.8%(11 cases) and 7.4%(64 cases) respectively. 3. In LA test serum antibody titers in 75 positive sera were shown as 54 cases(72.0% in 1:32, 9(12.0%) in 1:64, 7(9.4%) in 1:128, 3(3.40%) in 1:256, 1(1.3%) in 512 and 1(1.3%) in 1:2,048 respectively. 4. Positive rates of toxoplasma antibodies in cattls sera from each area were 0.2∼22.0%.

  • PDF

Production of Antibody against Saikosaponin a, an Active Component of Bupleuri Radix

  • Jung, Da-Woon;Sung, Chung-Ki
    • Archives of Pharmacal Research
    • /
    • v.21 no.2
    • /
    • pp.135-139
    • /
    • 1998
  • High titer rabbit polyclonal antibodies (pAbs) which show a specificity for saikosaponin a (SSA), have been generated. The immunogen used was a conjugate of SSA linked through its glucose moiety to bovine serum albumin by periodate oxidation method. The antibody titers obtained from two rabbits, innoculated with the immunogen, reached a plateau after the fourth and third booster injection, respectively. The specificity of the pAbs was determined by hapten inhibition assays using several SSA-like structures. SSA competitively inhibited the binding of the rabbit anti-SSA pAbs to SSA-ovalbumin on solid phase, a coated antigen on the well. The antibodies showed high specificity to SSA, exhibiting no significant cross-reactivity with any of SSA analogues tested.

  • PDF

A Case of Antisynthetase Syndrome (항 Synthetase 증후군 1예)

  • Kim, Min-Jeong;Kim, Min Ah;Kim, Eung-Gyu;Kim, Chan-Hwan;Kim, Sang-Jin
    • Annals of Clinical Neurophysiology
    • /
    • v.8 no.2
    • /
    • pp.196-198
    • /
    • 2006
  • It has been reported that antisynthetase syndrome belongs to the idiopathic myositis group which includes pulmonary interstitial disease, arthritis, Raynaud's phenomenon, and mechanic's hand, associated with the anti-Jo1 antibody. A 60- year-old man presented with one month history of lower limbs weakness, rapidly progressive exertional dyspnea, and arthralgia. A markedly increased titers of anti-Jo1 antibodies were found. Chest CT showed idiopathic pulmonary fibrosis. Muscle biopsies were consistent with polymyositis. A high dose corticosteroids and cyclosporine were not effective. We report a case of antisynthetase syndrome, in which immunosuppressive agents could not rescue the deteriorating disease course.

  • PDF

Immunomodulatory Effect of Auapuncture with Canine Parvovirus Vaccine (개 파보바이러스 백신 水針의 勉疫調節 효과)

  • 황수현;서지민;홍민성;최용수;송근호;김덕환;김명철;신광순
    • Journal of Veterinary Clinics
    • /
    • v.18 no.4
    • /
    • pp.368-373
    • /
    • 2001
  • To study the immunomodulatory effect of aquapuncture with canine parvovirus killed vaccine, the vaccine was inoculated into the dogs twice with on 2-week interval. The 6 dogs in the experimental group were inoculated through the Jiao-Chao acupoint, and 5 dogs in the control group were done subcutaneously. The antibody titer was determined by the hemagglutination inhibition test. The HI titers of the experimental group showed significantly higher on days 21 and 28 than those of the control group. The biochemical test on serum total protein, protein fractions and the A/G ration showed a slightly increased in $\gamma$-globulin on days 21 and 28. The hematological findings on total leukocytes and differential counts showed no significance. It was thought that the aquapuncture of the canine parvovirus killed vaccine through the Jiao-Cho acupoint may stimulate the antibody production.

  • PDF

Identification of a Strain of Babesia Isolated from Korean Cattle (한우(韓牛)로부터 분리(分離)한 바베시아 원충(原蟲)의 동정(同定))

  • Jeon, Yeong
    • Korean Journal of Veterinary Research
    • /
    • v.18 no.1
    • /
    • pp.27-31
    • /
    • 1978
  • In order to identify unknown Babesia spp. which was isolated from Korean cattle, the morphology of Korean strain was compared with that of Babesia bigemina (Kochinda strain) and Babesia spp. (Miyake strain). Immunofluorescent technique was used to identify the serological character of the parasites. The results obtained were summarized as follows: 1. Korean strain was morphologically very similar to Babesia spp.(Miyake strain) which mostly showed parallel-bigeminate forms, while B. bigemina (Kochinda strain) was mostly round and oval forms. 2. By the indirect fluorescent antibody technique: a) Anti-Babesia spp. and Korean Babesia spp. sera showed a higher antibody titers with Babesia spp. (Miyake strain) antigen (1:500) than with B. bigemina (Kochinda strain) antigen (1:50). b) Anti-Babesia bigemina sera showed a lower titer with Babesia spp. antigen (1:50) than with B. bigemina antigen (1:250). 3. On the basis of morphological and serological confirmantions, a Babesia strain isolated from a Korean cattle was very similar, if not identical, to Miyake strain of Babesia spp.

  • PDF

Prevalence of Leptospiral Antibodies in Sows (모돈에서의 Leptospira속균에 대한 혈중항체조사)

  • 강신석;곽학구;김홍기
    • Korean Journal of Veterinary Service
    • /
    • v.16 no.1
    • /
    • pp.65-69
    • /
    • 1993
  • A serological survey was conducted to detect the type of Leptospirosis in sows. Antigen(live antigen) tested were L.icterohemorrhagiae, L.pomonal, L.canicola, L.Hardjo, L.australis, L.autumnalis, L.grippotyphosa, L.pyrogens, L.bataviae, L.hebdomadis. The survey was performed from J one 1992 to December 1992 by microscopic-agglutination test. The results were as follows 1, Among the serum samples of 92 heads of the sows, 6 heads of the sow(6.5%) were positive. 2. Among the positive samples of 6 heads, L.icterihemorrhagiae were 4 heads(4.3%) and L. pomona 2 heads (2.2%), respectively. 3. Antibody titers of positive sera were ranging from 1:200 to 1:1600. 4. The seroprevalence of leptospira in Chechon city, Chechon county, Danyang county that Chechon county(3.3%) was higher than that of other districts(1% -2.2%).

  • PDF

A Cabe of Calabar Swelling Suspected as Loiasis (로아사상충증으로 생각되는 Calabar Swelling 치험 1예)

  • Min, Deuk-Yeong;So, Jin-Tak;Yun, Jin-U
    • Parasites, Hosts and Diseases
    • /
    • v.25 no.2
    • /
    • pp.185-187
    • /
    • 1987
  • Thia report deals with an imported case of calamar swelling suspective of loiasis, who had been in Nigeria, Africa for 2 years. This 33-year-old Korean technician was admitted to Severance Hospital, Yonsei University Medical Center, because of erythematous swellings on left hand and foot. His peripheral blood showed persistent eosinophilia (over 30% of WBC), increased IgE(1,000unit/ml) and significantly high antialarial antibody titer with ensyme immunoassay, although no microfilaria was detected on the periplleral blood films. Under the impression of Loa lea infection diethylcarbamasine was administered for a month. Four months later mobile swellings and eosinophilia disappeared, and anti-filarial antibody titers were normalized. It is assumed that the patient had suffered from Loa lea infection, which is the first report on loiasis in Korea.

  • PDF

Experimental infection in guinea pig with foot and mouth disease virus

  • Abdul-Ahad;Rahman, Md-Siddiqur;Rahman, Md-Mostafizur;Baek, Byeong-Kirl;Lee, John-Hwa
    • Korean Journal of Veterinary Service
    • /
    • v.26 no.1
    • /
    • pp.73-80
    • /
    • 2003
  • In order to obtain information on murine model for foot and mouth disease virus(FMDV) type Asia 1, we studied whether guinea pig was a suitable model for studying FMDV. Apparently healthy 3 months old albino guinea pigs and unweaned 3 days old Swiss albino mice were used for this study. Total of 8 guinea pigs were divided into the infected(n=5) and control(n=3) groups. The incubation period of FMDV in the guinea pigs were roughly 2 days and the viremia persisted for 3 days in the guinea pigs. Mice inoculated with the plasma from control guinea pigs did not show any sign of viremia. The plasma were titrated by virus neutralization test using suckling mice as an indicator host. The mean virus neutralizing antibody titers of infected guinea pig at 3 DPI, 4 DPI and 5 DPI were log$\_$10/2.16, log$\_$10/ 3.39 and log$\_$10/ 3.44, respectively whereas there was no neutralizing antibody titer in control group. The difference between the mortality pattern and mean virus neutralizing antibody titer of infected and that of control group at day 3, 4, 5 were statistically significant(p<0.0l).