• The Korean Journal of Blood Transfusion
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• v.29 no.3
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• pp.282-290
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• 2018

Background: Anti-E or paired anti-E/-c antibodies can develop in patients with the Rh CDe phenotype. This study examined the differences in transfusion in patients with the CDe phenotype according to formation of anti-E or anti-E/-c antibodies. Methods: Retrospective reviews were carried out on the results of antibody identification tests performed in 2014. The Rh phenotype and antibody specificity were investigated. The transfusion and medical records of patients with the CDe phenotype were examined. Results: In total, 76 patients were included in the review. Of these 76 patients, 38 (50.0%) were of the CDe phenotype. Anti-E antibodies were the most frequent (60.5%), followed by anti-E/-c antibodies (23.7%). The total transfusion units and platelet transfusion units were significantly higher in patients with anti-E/-c antibodies (P=0.028 and P=0.01, respectively). The distribution of categorized diseases was similar in the patients with the anti-E and anti-E/-c antibodies. A frequency of transfusion episodes greater than or equal to four was higher in patients with hepatobiliary diseases (85.7%). Conclusion: In CDe phenotype patients, platelet transfusion was significantly higher in the anti-E/-c positive group than the anti-E positive group, indicating that platelets play a role in red blood cell alloimmunization. Because E is the most immunogenic antigen in Korea, it is important to define the disease group, in which patients with CDe phenotype require a transfusion of E and c-negative blood.

• Korean Journal of Veterinary Service
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• v.42 no.1
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• pp.25-30
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• 2019

The objective of this study was to assess the possibility of detecting Foot-and-Mouth Disease Virus (FMDV) from the herd-based oral fluids specimens collected by the cotton ropes from pig farms that were found as FMDV nonstructural protein (NSP) antibodies positive. The cotton ropes were applied to detect FMDV in the selected pig farms which NSP antibodies were continuously detected in 2016, including the one pig farm which FMDV antigen were detected at the specimens from the pigsty environment. As the result, FMDV antigen were not detected in the oral fluid specimens collected by the cotton ropes. Theoretically, to detect FMDV antigen from the pigs with NSP antibodies has very low possibility because FMDV antigen disappeared at the time when NSP antibodies were produced by FMDV. Therefore, in order to detect FMDV antigen from the oral fluids using the cotton rope, it would be more effective to be applied to target the FMDV infected pigs rather than the NSP antibodies positive pigs. The collected oral fluids using cotton rope could be useful test specimens to monitor high-density pig populations for FMDV infection. Then, oral fluids sampling using cotton rope will be used for the efficient FMDV surveillance to detect FMDV antigen.

• BMB Reports
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• v.42 no.7
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• pp.418-420
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• 2009

We describe a method for producing polyclonal antibodies against peptide antigen cytochrome P450 1A2 and 3A4 using a tandem repeat of the epitope region and incorporation of proline residue between the repeated sequences. An ELISA assay revealed more efficient generation of polyclonal antibodies to tandem repeat peptide antigens than mono-epitope peptides. The incorporation of proline residues further stimulated antibody production.

• The Microorganisms and Industry
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• v.16 no.2
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• pp.2-9
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• 1990

This paper outlines research to study two aspects of Cryptooridium. First, specific antigenic determinants were identified and followed through the growth cycle of C. parvum to investigate antigenic sharing of molecular epitopes among the different life cycle stages. Secondly, the importance of passive immune protective mechanisms in cryptosporidial infection was assessed by following the course of infection in neonatal mice which have been subjected to treatments using either monoclonal antibodies (mAbs) or hyperimmune bovine colostrum.

• 대한핵의학회:학술대회논문집
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• 1979.05a
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• pp.4.2-5
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• 1979

• Korean Journal of Veterinary Service
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• v.17 no.1
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• pp.32-36
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• 1994

This study was carried out to investigate antibodies against Toxoplasma gondii by Latex agglutination test from 101 slaugutered pigs and 109 cattle. In the species, 5(4.6%) out of 109 cattle and 4(4.0%) out of 101 pigs were seropositive for Toxoplasma gondii.

• The Microorganisms and Industry
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• v.17 no.1
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• pp.2-9
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• 1991

All of four genes are cloned and DNA sequence analysis have now revealed that these four genes encode a family of proteins with similar amino acid sequence. These proteins show no extensive similarities to any known proteins (Haarer et al., 1991). Among them, CDC3 gene is fused with E. coli lacZ and trpE genes and antibodies against the CDC3 gene product are produced. These antibodies are used to check the localization of this product to the vicinity of the 10 -nm filaments in the mother-bud neck.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.270.1-270.1
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• 2003

Enzyme-linked immunoassay system using monoclonal antibody (MAb) has become an important methodology for the quantitative and qualitative analysis of drugs and phytochemical compounds having small molecular weight. In this study, monoclonal antibodies against berberine, one of main constituent of Phellodendron amurense, Coptis japonica and Corydalis turtschaninovii, were produced. (omitted)

• Korean Journal of Veterinary Research
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• v.43 no.2
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• pp.263-270
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• 2003

The objective of the present study was to investigate the use of fluid released from muscle samples as an alternative to serum for ELISA to detect classical swine fever(CSF) virus antibodies in slaughter pigs. The optimal correspondence between serum 1:20 OD values and muscle fluid OD values was achieved at a muscle fluid dilution of 1:2. Significant correlation was found between serum and neck muscle ELISA ($r_s=0.880$, p<0.0001, ${\kappa}=0.82$; specificity of 97.0% and sensitivity 90.6%). The semimembranous muscle showed similar correlation in CSF ELISA($r_s=0.877$, p<0.0001, ${\kappa}=0.75$; specificity of 94.1% and sensitivity 89.1%). High correlation was obtained between serum and mesenteric lymph node in the CSF ELISA ($r_s=0.937$, p<0.0001, ${\kappa}=0.87$; specificity of 97.1% and sensitivity 93.0%). Measmement agreement between serum ELISA and muscle fluid ELISA was calculated and expressed as limits of agreement. The correspondence of ELISA of serum and muscle fluid indicated limits of agreement. Above 95% of all muscle fluid values were distributed within this limits of agreement. Among the samples used for ELISA for detecting CSFV antibodies, mesenteric lymph node had the most correlation and agreement with serum ELISA. F-test for comparison of variances showed no significant difference between the serum and muscle fluid. In conclusion, muscle fluid is a useful postmortem alternative to serum to detect CSFV antibodies.

• Parasites, Hosts and Diseases
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• v.55 no.1
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• pp.99-103
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• 2017

Toxoplasma gondii is an important opportunistic agent especially in immunocompromised hosts and can cause significant morbidity and mortality. Hence, detection and monitoring of anti-Toxoplasma antibodies are of a great interest in HIV-infected patients. A study on the prevalence of toxoplasmosis and associated risk factors was carried out among HIV-infected patients in Jahrom, southern Iran. The prevalence of anti-Toxoplasma IgG antibodies was 21.1% in HIV-infected patients by ELISA. PCR was performed on all of the samples, and 1 of the blood samples was positively detected. Among the HIV patients, anti-Toxoplasma IgG antibodies were significantly higher in age group of 30-39 years old (P=0.05). The seroprevalence of toxoplasmosis in patients with $CD4^+$ < $100cells/{\mu}l$ was 33.3% that was significantly higher than the other groups (P=0.042) with or without IgG antibodies. The $CD4^+$ count mean of seropositive patients was lower than that of seronegative patients. The seroprevalence of toxoplasmosis in patients with highly active antiretroviral therapy was significantly less than patients without therapy (P=0.02). In conclusion, this study showed low seroprevalence of latent toxoplasmosis among HIV-infected patients in the region and confirmed the need for intensifying prevention efforts among this high-risk population and also the risk of toxoplasmosis reactivation which could be important among this population.