Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Application of cotton rope to detect foot-and-mouth disease virus in the pigs of farms in which nonstructural protein (NSP) antibody were detected in 2016

2016년 구제역 비구조단백질(NSP) 항체 지속 검출농가에서 구제역바이러스 검출을 위한 로프법 적용

  • Ha, Byeong-Suk (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Kim, Taeseong (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Lee, Jin-Woo (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Lee, Hyun-Ji (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Lee, Sumee (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Park, Hye-Jin (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Nah, Jin-Ju (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Ryoo, Soyoon (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Shin, Moon-Kyun (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Byun, Jae-Won (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Park, Mi-Young (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Pyo, Hyun-Mi (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Wee, Sung-Hwan (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency) ;
  • Nam, Yi-Hyun (Chungnam Veterinary Research Institute) ;
  • Lee, Seung-Yoon (HanByol Farm Tech) ;
  • Ku, Bok-Kyung (Foot and Mouth Disease Division, Animal and Plant Quarantine Agency)
  • 하병석 (농림축산검역본부 구제역진단과) ;
  • 김태성 (농림축산검역본부 구제역진단과) ;
  • 이진우 (농림축산검역본부 구제역진단과) ;
  • 이현지 (농림축산검역본부 구제역진단과) ;
  • 이수미 (농림축산검역본부 구제역진단과) ;
  • 박혜진 (농림축산검역본부 구제역진단과) ;
  • 나진주 (농림축산검역본부 구제역진단과) ;
  • 유소윤 (농림축산검역본부 구제역진단과) ;
  • 신문균 (농림축산검역본부 구제역진단과) ;
  • 변재원 (농림축산검역본부 구제역진단과) ;
  • 박미영 (농림축산검역본부 구제역진단과) ;
  • 표현미 (농림축산검역본부 구제역진단과) ;
  • 위성환 (농림축산검역본부 구제역진단과) ;
  • 남이현 (충남동물위생시험소) ;
  • 이승윤 (한별팜텍) ;
  • 구복경 (농림축산검역본부 구제역진단과)
  • Received : 2018.12.11
  • Accepted : 2018.12.26
  • Published : 2019.03.30
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Abstract

The objective of this study was to assess the possibility of detecting Foot-and-Mouth Disease Virus (FMDV) from the herd-based oral fluids specimens collected by the cotton ropes from pig farms that were found as FMDV nonstructural protein (NSP) antibodies positive. The cotton ropes were applied to detect FMDV in the selected pig farms which NSP antibodies were continuously detected in 2016, including the one pig farm which FMDV antigen were detected at the specimens from the pigsty environment. As the result, FMDV antigen were not detected in the oral fluid specimens collected by the cotton ropes. Theoretically, to detect FMDV antigen from the pigs with NSP antibodies has very low possibility because FMDV antigen disappeared at the time when NSP antibodies were produced by FMDV. Therefore, in order to detect FMDV antigen from the oral fluids using the cotton rope, it would be more effective to be applied to target the FMDV infected pigs rather than the NSP antibodies positive pigs. The collected oral fluids using cotton rope could be useful test specimens to monitor high-density pig populations for FMDV infection. Then, oral fluids sampling using cotton rope will be used for the efficient FMDV surveillance to detect FMDV antigen.

Keywords

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Fig. 1. Suspending a cotton rope in a pig pen for 1hr and collected by squeezing the rope.

Table 1. Results of 1st Samples collected from 11 farms in Chungnam area

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Table 2. Results of 2nd Samples collected from 11 farms in Chungnam area

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Table 3. Results of Samples collected from 2 farms in Chungnam area

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