• The Journal of the Korean Society for Microbiology
• /
• v.20 no.1
• /
• pp.13-23
• /
• 1985

This investigation was performed to isolate and identify Staphylococcus sp. from air at the 25 sites in one hospital in Daegu. Drug sensitivity tests bacteriologically were also studied. The results are summarized as follows: 1. In gram staining of isolated colonies, the number of staphylococci strains was 959(70.2%) among 1367 collected in the morning, and 653(66.2%) among 987 collected in the afternoon. 2. The number of isolates was slightly higher in samples collected from the outpatient treatment rooms and wards in the main building than in samples from the other sites. 3. In biochemical tests of 1612 strains of isolates gram positive cocci, the number of coagulase positive strains was 584(36.2%) and coagulase negative 1028(63.8%). In experiments on growth on mannitol agar containing 7.5% NaCl and mannitol fermentation tests, the number of mannitol fermentation strains was 423(30.8%) and nonfermenter was 951(59.2%). 4. In antibiotic sensitivity tests of 746 identified strains of staphylococci, coagulase positive strains showed resistance in. higher portions to penicillin(89.7%), chloramphenicol(64.6%), gentamycin (52.1%) and tetracycline(45.2%), whereas in lower portions to kanamycin(39.2%) and apmicillin(33.1%). 5. Among coagulase negative and mannitol fermenting strains, higher portions showed resistance to chloramphenicol(76.5%), penicillin(69.8%) and ampicillin(63.6%), whereas lower portions showed resistance to kanamycin(48.8%), tetracycline(39.5%) and gentamycin(34.0%). 6. The number of strains showing multiple resistance to above 6 antibiotics were 15(52(19.8%) among coagulase positive and mannitol non-fermenter and 36(22.2%) among coagulase negative and mannitol fermenter.

• Journal of Veterinary Clinics
• /
• v.16 no.1
• /
• pp.214-217
• /
• 1999

An 8-month-old male orangutan (Pongo pygmaeus) housed in Everland Zoo-logical Gardens died after having 10 day history of depression, anorexia and acute respiratory distress. On necropsy, the lung failed to collapse and showed diffuse red consolidation. Microscopically, the alveoli were filled with large amounts of red blood cells, fibrin and varing number of hemosiderin-laden macrophages. The alveolar septa were thickened due to infiltrated mononuclear cells and congestion and are occasionally necrotic. Escherichia coli was isolated from the lung, spleen and kidney. The result of antibiotic sensitivity test of the isolated organism was also described.

• Journal of Yeungnam Medical Science
• /
• v.37 no.1
• /
• pp.54-58
• /
• 2020

Achromobacter xylosoxidans is a gram-negative bacterium that can oxidize xylose. It is commonly found in contaminated soil and water but does not normally infect immunocompetent humans. We report a case of a cavitary lung lesion associated with community-acquired A. xylosoxidans infection, which mimicked pulmonary tuberculosis or lung cancer in an immunocompetent man. The patient was hospitalized due to hemoptysis, and chest computed tomography (CT) revealed a cavitary lesion in the superior segment of the left lower lobe. We performed bronchoscopy and bronchial washing, and subsequent bacterial cultures excluded pulmonary tuberculosis and identified A. xylosoxidans. We performed antibiotic sensitivity testing and treated the patient with a 6-week course of amoxicillin/clavulanate. After 2 months, follow-up chest CT revealed complete resolution of the cavitary lesion.

• Journal of Veterinary Clinics
• /
• v.23 no.4
• /
• pp.476-479
• /
• 2006

A 9-year-old, female Yorkshire terrier and a 10-year-old, female Maltese were referred to the Veterinary Medical Teaching Hospital of Konkuk University. The dogs were presented with severe swelling and cutaneous sinus tract on the left suborbital lesion. Under general anesthesia, we performed physical, intraoral examinations and survey radiography, and diagnosed as periapical abscess in a left fourth premolar tooth. They were treated with tooth extraction, and the sinus tracts were drained with 0.05% chlorhexidine solution. After teeth extractions, medication applied by the result of antibiotic sensitivity test. During follow-up 10 months later, the left suborbital swelling had completely resolved.

• Bulletin of the Korean Chemical Society
• /
• v.35 no.11
• /
• pp.3195-3198
• /
• 2014

An ultra-sensitive detection method for small molecules such as antibiotics was developed using ICP-MS with magnetic and $TiO_2$ nanoparticles. Since most of the antibiotics are too small to employ a sandwich-type extraction through an immunoreaction, a non-specific platform was employed, in which the target was extracted by magnetic separation, followed by tagging with $TiO_2$ nanoparticles of 11.2 nm for ICP-MS measurement. The detection limit for salinomycin obtained from spiked serum samples was $0.4ag\;mL^{-1}$ (${\pm}10.3%$), which was about $1.5{\times}10^6$ times lower than that of LC-MS/MS and about $1.2{\times}10^{11}$ times better than that of ELISA. Such an excellent sensitivity enabled us to study the toxicity of antibiotics exposed to human beings by determining them in serum.

• Journal of Life Science
• /
• v.9 no.5
• /
• pp.564-569
• /
• 1999

This study was conducted to determine the concentrations of plant growth regulators required for regeneration and the concentrations of antibiotics for the selection of transformed regenerants from lettuce, musk melon and tomato. The optimal concentrations of plant growth regulators for shoot formation were NAA 0.1 mg/$\ell$ +BA 0.1 mg/$\ell$ for lettuce, NAA 0.01 mg/$\ell$ +BA 2.0 mg/$\ell$ for melon and NAA 0.1 mg/$\ell$ +BA 0.5 mg/$\ell$for musk melon. Shoot induction from tomato, lettuce and melon was completely inhibited by 30 mg/$\ell$ or higher concentrations of kanamycin. Shoot formation from mu나 melon was not affected by kanamycin up to 40 mg/$\ell$, but was reduced in the presence of 50 mg/$\ell$ and completely inhibited by 100 mg/$\ell$. Shoot formation of all four crops was completely inhibited by higromycin at 10 mg/$\ell$. Both carbenicillin and cefatoxinme did not show any negative effects on shoot formation.

• Korean Journal of Animal Reproduction
• /
• v.9 no.1
• /
• pp.66-71
• /
• 1985

After the induction of estrus by the PGFF2$\alpha$-GTH combination on the 118 repeat breeder cow during of 6-13 days of the estrus cycle, the effects of GTH, antibiotics, antibiotics-GTH combination were compared. The results of this study, the following conclusion were obtained: 1. The rate of estrus was reduced the administration of GTH before the administration of PGF2$\alpha$. 2. The rate of ovulation delayed was 24.6% in the group with the administration GTH, but 34.1% without the administration GTH. Duration of ovulation was shortened by GTH. 3. The impregnation induction by the administration of PGF2$\alpha$, PGF2$\alpha$-antibiotics, PGF2$\alpha$-GTH-antibiotics were 41.7%, 69.5%, 55.6% and 80.8% respectively injection in the uterus was more effective than the administration. Before or after the A.I., the above combination was most effective. 4. In antibiotics sensitivity test in vitro of the intravagina bacteria, 5.0%, 12.7% and 60.0% of bacteria were sensitive to Penicilline, Streptomycin and Gentamicin respectively.

• Korean Journal of Veterinary Service
• /
• v.16 no.1
• /
• pp.76-81
• /
• 1993

To evaluate the pathogenesis of live infectious laryngotracheitis vaccine, virus, virus and E.coli were inoculated in 4-weeks old intraorbitally and intraorally. Their pathology and serology were studied. The results were as follows. 1. thicks Inoculated with ILT vaccine alone showed no clinical sign. 2. Some of chicks inoculated with ILT vaccine followed by E.coli after 1day showed nasal discharge and cough. 3. Some of chicks inoculated with E. coli followed by ILT vaccine after 1 day and inoculated ILT vaccine and E.coli concurrently, showed nasal discharge. 4. Serum neutralization titers of chicks inoculated with ILT vaccine increased 3 weeks after Inoculation, peaked at 7 to 8 weeks and decreased. 5. Antibiotic sensitivity of E.coli were higher in Enrofloxacin (Baytril) and chloramphenical.

• Journal of Veterinary Clinics
• /
• v.37 no.3
• /
• pp.157-162
• /
• 2020

A 6-year-old Thoroughbred mare presented to the Korea Racing Authority Equine Hospital with dropping of the left front fetlock due to an injury sustained while racing. Radiographic examination revealed a comminuted fracture of both proximal sesamoid bones of the affected fetlock. Arthrodesis of the fetlock joint using a broad dynamic compression plate with a tension band wire was performed as a salvage procedure for the future use as a broodmare. After surgery, however, a delayed union of the bones and surgical site infection was present for a prolonged period. Staphylococcus aureus was persistently identified from the surgical site, and antimicrobial therapies were based on antibiotic sensitivity tests, including regional perfusions. The removal and replacement of surgical implants associated with seropurulent discharge was based on coordinating the development of fetlock ankylosis and infection control over 13 months. Firstly, seven screws associated with surgical drainage were replaced and bone morphogenetic protein-2 (BMP-2) and local antibiotics were placed into the surgical site to accelerate bone fusion at postoperative month 7. Further six screws, along with drainage, were removed at postoperative month 10. The plate and screws were removed from the limb due to the progression of bone fusion at postoperative month 13; BMP-2 and local antibiotics were also used. Delayed healing of arthrodesis due to surgical site infection and implant instability were treated by implant removals and antibiotic therapies, and the horse eventually showed improved weight-bearing ability of the affected limb.

• Journal of Animal Science and Technology
• /
• v.50 no.6
• /
• pp.793-798
• /
• 2008

The semen collection process in the porcine is far from being a sterile procedure. Consequently, porcine ejaculates commonly contain bacterial contaminants. The aim of this study is to identify the bacteria in porcine semen and to find the antibiotics resistance of bacteria. Twelve porcine originating from four AI center were used to collect semen. Bacteria were identified by automated instrument for rapid organism identification system and bacterial sensitivities of 8 antibiotics were tested. The Bacterial contaminants of Staphylococcus genus(37.8%), Proteus genus(7.0%), Bacillus genus (6.1%), Pasteulla genus(5.7%), Acinetobacte genus(5.2%), Serratia genus(4.3%) and others(33.9%) were frequently isolated. However, amikacin showed higher antibiotic sensitivity than other antibiotics. General sanitation protocols can contribute partly to inhibit the bacterial contamination, with monitoring boar housing, semen collection areas and the extended semen. But, proper selection of preservative antibiotics by microbial sensitivities can minimize the influence of bacteria.