• 약학회지
• /
• 제44권1호
• /
• pp.16-21
• /
• 2000

Peptic ulcer is involved with Helicobacter pylori infection and antibiotic regimens are primary treatments. An optimal therapeutic regimen for eradication of Helicobacter pylori remains uncertain due to variable efficacy. The objectives of this study were to evaluate the efficacy of omeprazole based antibiotic regimens in bacterial eradication, healing of peptic ulcer and to identify factors affecting efficacy. Seventy-seven patients were enrolled in the prospective, open-trial from November 1997 to Setember 1998. H. pylori infection was identified with endoscopy, H. pylori stain and rapid urease test. The first group (OAC7) received omeprazole 20 mg twice daily for 4 weeks which were the same schedule for all, amoxicillin and clarithromycin 500 mg three times daily for 1 week; the second group (OAC14), for 2 weeks on the same regimen as the first; and the last group (OACD) has taken bismuth in addition to the OAC7 regimen for 1 week. Eradication of H. pylori and healing of peptic ulcer were evaluated with endoscopy and tests for H. pylori before and after treatments. There were no significant differences in eradication rates; 77% in OAC7, 76% in OAC14, 81% in OACD (p=0.935) and healing rates; 82% in OAC7, 71% in OAC14, 95% in OACD (p=0.179), respectively. Compliance had an relationship with eradication rates significantly among regimens (p=0.049). Twenty three cases (29%) complained of the minor side effects. In conclusion, OAC7 was better in convenience of dosing schedule and showed fewer side effects with shorter duration and lower cost while There were no significant differences in efficacy among regimens.

• 한국미생물·생명공학회지
• /
• 제10권3호
• /
• pp.163-169
• /
• 1982

인삼 saponin과 항생 물질의 사용에 따른 in vivo에서 출현 가능한 영향을 in vitro에서 미생물을 대상으로 검토하였다. aminoglycoside 항생 물질인 streptomycin·sulfate, kanamycin·sulfate, gentamycin·sulfate 등은 인삼 saponin과의 상호 작용으로 그 항균 활성이 대체로 상승되었으며 Erwinia aroideae에 대해서만 그 항균활성이 항상 상승되지는 않는 경향을 나타내었다. benzene 유도체항생 물질인 chloramphenicol은 인삼 saponin과의 상호 작용으로 그 항균활성이 Salmonella typhi. Aerobacter aerogenes, Serratia속 세균등에서는 상승하였으나 Bacillus suhtilis, Bacillu, megaterium, Escherichia coli균에서는 활성이 감소되었고 그 밖의 세균에서는 항균활성에 변화를 나타내지 못하였다.

• 한국임상수의학회지
• /
• 제25권4호
• /
• pp.231-235
• /
• 2008

Various kinds of antibiotic intramammary infusions are used for treatment of bovine mastitis. As antibiotic-resistant bacteria are increased, the therapeutic rate for bovine mastitis is decreased. The goal of this research is to detect significant synergic effects of combination of antibiotics with sulbactam, $\beta$-lactamase inhibitor, on methicilin-resistant Staphylococcus aureus (MRSA). We used 5 strains of MRSA isolated from bovine mastitis with clinical and subclinical signs. All of the bacteria isolated had resistance to oxacillin and showed multi-resistant patterns in the antimicrobial susceptibility tests. Minimal bactericidal concentrations of ampicillin, amoxicillin, cephalexin, ampicillin/sulbactam(2:1), amoxicillin/sulbactam (2:1), and cephalexin/sulbactam (1:1) were measured according to broth microdilution method suggested by National Committee for Clinical Laboratory Standards (NCCLS, M31-A2) to compare the synergic effects of sulbactam combination with each antibiotic alone. Ampicillin and amoxicillin showed synergic antibacterial activity to 4 and 3 respectively in 5 strains of MRSA in combination with sulbactam. This study demonstrates that ampicillin/sulbactam and amoxicillin/sulbactam can be therapeutic choices for mastitis associated with MRSA.

• Journal of Microbiology and Biotechnology
• /
• 제9권4호
• /
• pp.488-497
• /
• 1999

Probing Streptomyces albus ATCC 21838 chromosomal DNA with a proline tRNA sequence resulted in an isolation of a putative integrating element in the 6.4-kb EcoRI fragment. It was found that Streptomyces lividans TK-24 transformed with a cloned DNA fragment on a multicopy plasmid, produced a higher level of spore pigment and mycelial red pigment on a regeneration agar. Furthermore, the transformant S. lividans TK-24 produced a markedly increased level of undecylprodigiosin in a broth culture. A nucleotide sequence analysis of the cloned region revealed several open reading frames homologous to the integrases of integrating plasmids or temperate bacteriophages, signal-transducing regulatory proteins with a conserved ATP-binding domain, oxidoreductases ($\beta$-ketoacyl reductase), and an AraC-like transcriptional regulator. To examine the effect on antibiotic production, each coding region was overexpressed separately from the other genes in the region in S. lividans TK-24 with; pJHS3044 for the expression of the signal-transducing regulatory protein homologue, pJHS3045 for the homologue of oxidoreductase, and pJHS3051 for the homologue of the AraC-like transcriptional regulator. Phenotypic studies of S. lividans TK-24 strains harboring plasmids for the overexpression of individual genes suggested the following effects of the genes on antibiotic production: The oxidoreductase homologue stimulated the production of actinorhodin and undecylprodigiosin, which was influenced by the culture conditions; the homologue of the AraC-like transcriptional regulator was the most effective factor in antibiotic production within all the culture conditions tested; the signal-transducing regulatory protein homologue repressed the effect due to the homologue of the AraC-like transcriptional regulator, however, the antibiotic production was derepressed upon entering the stationary phase.

• 대한치과의사협회지
• /
• 제51권3호
• /
• pp.148-155
• /
• 2013

Antibiotic is one of the mainly prescribed drugs in dental office. The substantial part of all antibiotics used in dental office is given to patients without signs or symptoms of infection to prevent infections, and antibiotics became the most widely abused prescribed drugs on the basis of inappropriate indications, dosages and durations. Considering that antibiotics are one of the drugs that affect not only a single patient but also entire populations of individuals through their collective effects on microbial ecology, the importance of proper use of antimicrobial therapy can hardly be overemphasized. Therefore, the main considerations for antibiotic therapy in denial office were summarized here.

• 대한한의학방제학회지
• /
• 제23권1호
• /
• pp.141-150
• /
• 2015

Objectives : This study is to report the effects of Bangkibokryeong-tang(Fanjifuling-tang(BBT)) on stroke patient with Urinary tract infection.(UTI) Methods : A stroke patient diagnosed as UTI was treated with BBT (防己茯笭湯) at one-month intervals. We observed body temperature, amount of urine and recorded photographs of any changes during the treatment period. Treatment efficacy was assessed using urinary analysis. Results : After the treatment, there was improvement in urine analysis. And the symptoms of UTI and her conditions got better. Conclusion : The report suggests that BBT is effective in controlling various symptoms related to UTI with antibiotic resistance on stroke patient.

• 약학회지
• /
• 제42권2호
• /
• pp.229-231
• /
• 1998

The post-antibiotic effects (PAE) of DW-116 were evaluated against Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 27348, Staphylococcus aureus ATCC 25923 , Escherichia coli ATCC 25922 and Pseudomonas aeruginosa MB4-16, repectively. Against gram-positive bacteria, PAEs of DW-116 were longer duration (20-35 min) than those of rufloxacin(1O-20 min), and shorter than those of ciprofloxacin (50-90 min). Especially, against E. coli, DW-116 and ciprofloxacin obtained approximately 3 hrs of PAES.

• 약학회지
• /
• 제56권5호
• /
• pp.320-325
• /
• 2012

To develop a new effective and safe natural antibiotics and antioxidant the essential oil was extracted from the roots of Anthriscus sylvestris by steam distillation. Its composition was analyzed by GC-MS. The activities of the essential oil fraction and its main components were evaluated against antibiotic-susceptible and -resistant strains of some food-born bacteria. In addition the synergism was examined with this oil combined with antibiotic by checkerboard titer test. The antioxidant activities were determined by in 1,1-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging activity test and reducing power assay. The essential oil fraction of A. sylvestris revealed significant inhibiting activities against antibiotic-susceptible and -resistant species of Vibrio and Shigella with MICs ranged from 1.00~4.00 mg/ml. It showed synergistic or additive effects when it was combined with amphicillin or trimethoprim/sulfamethoxazole (1 : 9). Additionally, the essential oil fraction of A. sylvestris exhibited significant DPPH free radical scavenging activity and the reducing power.

• Biomolecules & Therapeutics
• /
• 제7권1호
• /
• pp.97-104
• /
• 1999

A new antibiotic bead, CJ-40003 is a combination of three antibiotics, tobramycin, vancomycin and cefazolin embedded in bone cement, for the treatment of osteomyelitis. To evaluate the general pharmacological properties of CJ-40003, the effects of its active ingredients were investigated in mice, rats, dogs and isolated guinea pig ileum. The combination of three antibiotics (CA) did not affect general behavior, central nervous system, smooth muscles, gastrointestinal system, cardiovascular and respiratory system and water and electrolytes excretion when administered intravenously at the doses of 0.3, 1 and 3 mg/kg, respectively, into experimental animals. The CA had no effect on the contractile response of the isolated guinea pig ileum to various spasmogen at concentrations of 1, 3 and 10 $\mu\textrm{g}$/ml, respectively. In conclusion, the active ingredients of CJ-40003 showed no pharmacological effect in these studies.

• Childhood Kidney Diseases
• /
• 제20권1호
• /
• pp.1-5
• /
• 2016

Since many years, continuous low dose antibiotic prophylaxis (CAP) has been used for children at a risk for recurrent urinary tract infection (UTI), especially those with vesicoureteral reflux (VUR). The incidence of recurrent UTI has been shown to be higher in children with VUR with bladder and bowel dysfunction (BBD) than in those with VUR without BBD. Therefore, CAP has been recommended for children with BBD and VUR because of the increased risk of UTI. However, the use of CAP has become highly controversial because of bacterial resistance developed due to antibiotic over-usage. The preventive effects of probiotics have been proved in various adult urogenital infections, and the antimicrobial activities of lactobacilli against uropathogens have been demonstrated in previous in vitro studies. However, a critical assessment of their efficacy in children with UTI is lacking. The importance of the use of urogenital probiotics is that it is a natural approach that replenishes the depleted normal flora to create a better environment to fight off uropathogens. Probiotics have a great potential, particularly today with the increasing threat of antibiotic-resistant microorganisms.