• Title/Summary/Keyword: Antibacterial study

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Antibacterial Effects of Major Compounds in Essential Oil against Fish Disease Bacteria (식물 정유 주요 성분의 어병 세균에 대한 항균활성)

  • Kyoung-In, Lee;Geun-Jik, Lee;Young-Seung, Yoon;Byoung Sik, Pyo
    • Journal of Marine Bioscience and Biotechnology
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    • v.14 no.2
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    • pp.86-92
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    • 2022
  • In this study, the antibacterial activity of major compounds obtained from domestic plant essential oils was investigated against four species of fish pathogenic bacteria, namely, Edwardsiella tarda, Vibrio harveyi, Streptococcus iniae, and Streptococcus parauberis. We observed that α-terpineol and eucalyptol showed high antibacterial activity against S. iniae, whereas α-terpineol and β-linalool showed high antibacterial activity against S. parauberis. The antibacterial activity of eucalyptol and β-linalool was significantly high against E. tarda, and α-terpineol and β-linalool exerted antibacterial activity against V. harveyi. All compounds showing significant antibacterial activity also exhibited high solubility in water (≥1,000 mg/L). In contrast, compounds such as α-limonene and α-pinene with low water solubility showed significantly low antibacterial activity against all bacteria. These findings can be utilized further to estimate the antibacterial activity of compounds isolated from plant essential oils for the prevention of fish disease.

Antibacterial and virucidal activity of 28 extracts from plants endemic to Korea against Bacillus cereus, Staphylococcus aureus, and murine norovirus

  • Hyun-Joo Chang;Ji Hye Han;Nari Lee;Sung-Wook Choi
    • Journal of Applied Biological Chemistry
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    • v.66
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    • pp.345-352
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    • 2023
  • Antibacterial activity against foodborne bacteria (Bacillus cereus, Staphylococcus aureus, Salmonella Enteritidis) and inhibitory activity against murine norovirus, a human norovirus surrogate, of 28 extracts from plants endemic to Korea were investigated in this study. All plant extracts showed antibacterial activity only against gram-positive bacteria, B. cereus and S. aureus. Extracts from Callistemon speciosus and Nymphaea tetragona showed inhibition zones of 16.54 and 24.35 mm against B. cereus and S. aureus, respectively, presenting the highest antibacterial activities recorded in this study. Among all samples, Ardisia japonica extract at concentrations of 100 and 200 ㎍/mL showed the highest virucidal activities of 96.6 and 100.0%, respectively. Ardisia japonica, Duchesnea indica, Polygonum aviculare, and Geum japonicum extracts showed high antibacterial and virucidal activity simultaneously without Raw 264.7 cell cytotoxicity. These plant extracts may serve as potential antimicrobials to control foodborne infections.

A Study of high Efficiency anti-Bacteria Filters for Passenger Car (객차용 고효율 항균 기능성 필터에 관한 연구)

  • 정우성;박덕신;정진도;류해열
    • Proceedings of the KSR Conference
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    • 2002.10b
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    • pp.975-980
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    • 2002
  • We actually installed antibacterial air filter on the air intake route to air conditioner in passenger car and evaluated filters performance on antibacterial effect. And also, antibacterial effect was observed at whole are of filter media by zone of inhibition test. In the evaluation of antibacterial test, it was shown that the air filter has notably sufficient antibacterial efficiency against ordinary filter.

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Antibacterial potential of the extracts derived from leaves and in vitro raised calli of medicinal plants Pterocarpus marsupium Roxb., Clitoria ternatea L., and Sanseveiria cylindrica Bojer ex Hook

  • Shahid, M;Shahzad, A;Anis, M
    • Advances in Traditional Medicine
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    • v.9 no.2
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    • pp.174-181
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    • 2009
  • Pterocarpus marsupium, Clitoria ternatea, and Sanseveiria cylindrica are some of the important and endangered medicinal plant species of India. Despite of medicinal properties, antibacterial potential of the plants have not yet been explored. The present study was designed to optimize the in vitro technique for micropropagation and to screen the extracts from leaves and in vitro raised calli for antibacterial properties. Excised leaf-explants from the parent plants were surface sterilized and cultivated on Murashige & Skoog's (MS) medium containing $N^6$-benzyladenine (BA) in concentrations of 1, 2, 5, and $10{\mu}M$. Optimal growth of calli was noticed at a concentration of $5{\mu}M$, therefore the extracts from calli grown at this concentration were further studied for antibacterial activity. Both alcoholic and aqueous extracts from leaves of respective plants, and their in vitro raised calli were tested for antibacterial activity by agar well diffusion method against a range of Gram-positive and Gram-negative bacteria. Aqueous extracts showed antibacterial activity against limited number of bacterial species; notably the extracts of C. ternatea which showed antibacterial activity against Streptococcus pyogenes, Bacillus subtilis and Bacillus cereus. Alcoholic extracts of all three plants showed antibacterial activity against a wider range of bacteria. Among the Gram-positive bacteria, extracts from C. ternatea showed strong antibacterial activity against Bacillus spp., whereas the extracts of S. cylindrica showed good antibacterial potential for Staphylococcus aureus, S. epidermidis and S. pyogenes. The extracts from all three plants showed antibacterial activity against Gram-negative bacteria, including, Salmonella spp. and Shigella dysenteriae; organisms causing enteric fever and dysentery. In most of the cases, the extracts from respective calli showed comparable, and in some cases better, result in comparison to the extracts from parent leaves. To the best of our knowledge this is the first preliminary report on antibacterial potential, especially through calli extracts, of these plants; and in vitro cultivation of the explants may be used to obtain phytotherapeutic compounds.

A Study on the Antibacterial Activity of Chitosan on the MRSA by the AATCC Test Method 100 and Modified AATCC Test Method 100

  • Choi, Jeong-Im;Jeon, Dong-Won
    • Fashion & Textile Research Journal
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    • v.4 no.6
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    • pp.557-563
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    • 2002
  • Water-soluble chitosan and water-insoluble chitosan with molecular weight of 2,000,000, 500,000, 80,000, and 40,000 with more than 90%of degree of deacetylation were produced to test antibacterial activity of chitosan against a pathogenic bacteria, Methicillin Resistant Staphylococcus aureus(MRSA). the AATCC Test Method 100and Modified AATCC Test Method 100 were used to evaluate the antibacterial activity of chitosan. Antibacterial activity of chitosan/acetic acid solution was the same when they were tested by two different methods, but those of polyester fabrics treated with chitosan/acetic acid solution were different in different antibacterial test. So several problems were found in the experimental methods. The AATCC Test Method 100 seems that excessive nutrition exists in inoculum solution by quantitative analysis on the basis the result of antibacterial activity on chitosan/acetic acid solution and amount of chitosan attached to the surface of treated fabrics.

Antibacterial Activity and Inhibitory Effect of Biofilm Formation by Actinidia polygama against S. mutans and P. gingivalis

  • Chung Mu Park;Hyun-Seo Yoon
    • Biomedical Science Letters
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    • v.29 no.2
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    • pp.88-94
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    • 2023
  • In oriental medicine, the fruit of Actinidia polygama has long been used to alleviate the symptoms of gout, arthritis, and inflammation. In this study, it was to designed to analyze the antibacterial activity of A. polygama ethanol extract (APEE) against Streptococcus mutans, one of the major strains for dental caries, and Porphyromonas gingivalis, one of the critical strains for periodontal disease. The antibacterial activity of APEE was analyzed by disk diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. In addition, it was also analyzed the inhibitory effect of APEE on bacterial growth and biofilm formation against both oral pathogens. APEE exhibited its antibacterial effect through the inhibited bacterial diffusion as well as low concentration of MIC and MBC. In addition, APEE significantly inhibited not only bacterial growth but also biofilm formation in a dose-dependent manner. Consequently, APEE showed potent antibacterial activity against both S. mutans and P. gingivalis, which indicates that APEE might be used as a potential antibacterial material for the improvement of oral healthcare.

A Study of Development Antibacterial Cosmetic Case using UV Wavelength

  • Yum, Seung Hoon;Jung, In Geung;Jeong, Jae Young;Hwang, You Jin
    • Journal of the Korea Society of Computer and Information
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    • v.25 no.5
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    • pp.179-186
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    • 2020
  • In this study, we propose an antibacterial cosmetic case using UV wavelengths. UVC have excellent antibacterial strength in terms of economics and convenience. The purpose of experiments was to prove it and to select a suitable permeability material. In experiments, UVA, UVC machanics and the competitor product were used to measure and compare the antibacterial strength of antibacterial cosmetic case using UV wavelength. S. aureus, a gram-positive strain, was used subject to the experiment, and the antibacterial strength measured and compared the number of colonies using the plate count technique. As a result, both UVA and UVC showed antibacterial activity, and UVC showed the most powerful antibacterial activity. In Conclusion, the Quartz glass was the most suitable of the permeable materials, but the more thick the examined quartz material became the less antibacterial strength.

Antibacterial activity of supernatant obtained from Weissella koreensis and Lactobacillus sakei on the growth of pathogenic bacteria

  • Im, Hana;Moon, Joon-Kwan;Kim, Woan-Sub
    • Korean Journal of Agricultural Science
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    • v.43 no.3
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    • pp.415-423
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    • 2016
  • This study was carried out to obtain basic data for the industrial use of Weissella koreensis and Lactobacillus sakei. The antibacterial activity of supernatants obtained from W. koreensis and L. sakei were tested against pathogenic bacteria such as Escherichia coli KCCM 11234, Salmonella enteritidis KCCM 3313, Salmonella enteritidis KCCM 12021, Salmonella typhimurium KCCM 40253, and Salmonella typhimurium KCCM 15. The supernatant of L. sakei showed antibacterial activity against E. coli KCCM 11234, S. enteritidis KCCM 12021, and S. typhimurium KCCM 15, while the supernatant of W. koreensis showed antibacterial activity against E. coli KCCM 11234 and S. enteritidis KCCM 12021. The effect of pH changes and heat treatment on antibacterial activity of the supernatants was examined using the sensitive pathogenic bacteria (E. coli KCCM 11234, S. enteritidis KCCM 12021 and S. typhimurium KCCM 15). Antibacterial activity against sensitive pathogenic bacteria was maintained under heat treatment at all temperatures, but there was no antibacterial activity associated with pH modification. Furthermore, it was confirmed that the antibacterial activity of the supernatants obtained from W. koreensis and L. sakei was a result of organic acids including, lactic, acetic, phosphoric, succinic, pyroglutamic, citric, malic, and formic acids. Therefore, the present study showed that the organic acids produced by L. sakei and W. koreensis exhibited a strong antibacterial activity against pathogenic bacteria. Moreover, in the food industry, these organic acids have the potential to inhibit the growth of pathogenic bacteria and improve the quality of stored food.

Evaluation of the physical properties and antibacterial effects on Candida albicans of denture base resin containing silver sulfadiazine (실버 설파다이아진이 첨가된 의치상용 레진의 Candida albicans에 대한 항균평가 및 물성 평가)

  • Yu-Ri Choi;Min-Kyung Kang
    • Journal of Korean society of Dental Hygiene
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    • v.23 no.6
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    • pp.459-466
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    • 2023
  • Objectives: The purpose of this study was to evaluate the physical properties and antibacterial activity of denture base resin with added silver sulfadiazine. Methods: Specimens were made from self-curing denture base resin and silver sulfadiazine as an inorganic antibacterial agent. For physical evaluation of the specimens, surface roughness, surface hardness, and contact angle were measured. Bacterial growth was assessed by optical densityat 600 nm (OD600) and colony forming units (CFU) measurements to confirm antibacterial activity. Results: There was no significant difference in surface roughness, surface hardness, and contact angle in the experimental group containing silver sulfadiazine compared to the control group. In contrast, the experimental group showed a significant decrease in antibacterial activity compared to the control group in terms of OD value. Analysis of CFU confirmed a significant decrease in colonies in the experimental group compared to the control group. Conclusions: Denture base resin containing silver sulfadiazine, an inorganic antibacterial agent, exhibited enhanced antibacterial activity without physical changes. In conclusion, the use of denture base resin containing inorganic antibacterial agents may be expected in the future.

Characterization of Antibacterial activity and Synthesis of Sulfanilamide Polymer using Crosslinking Agent (가교제를 이용한 Sulfanilamide 중합체의 합성과 항균특성)

  • Kim, Jong-Woan;Yoon, Chul-Hun;Hwang, Sung-Kwy;Kong, Seung-Dae;Lee, Han-Seab
    • Journal of the Korean Applied Science and Technology
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    • v.17 no.1
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    • pp.37-42
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    • 2000
  • Drug delivery system(DDS) have been actively studied for the past twenty years. Dual action agents are unique chemical entities comprised of two different types of antibacterial compounds covalently linked together in a single molecule in such a way that both components are able to exert their bactericidal properties. In spite of the advent of the antibacterial agent the sulfa agents are the most widely used antibacterial agent today. In this study, new antibacterials derivative was synthesized using glutaraldehyde such as crosslinking agent for the purpose of dual-action as DDS study. Antibacterial activity of these new synthetic derivative between their structures and activities were examined by disc diffusion method. As a result, new synthetic derivative exhibited the broad antibacterial activities against Gram(+) and Gram(-) bacilli. Especially, the antibacterial effect of new synthetic derivative against Gram negative(Esherichia. coli) was much stronger than that against Gram positive.