• Journal of Ginseng Research
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• 제35권3호
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• pp.352-359
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• 2011

Korean red ginseng (KRG) is used worldwide as a popular traditional herbal medicine. KRG has shown beneficial effects on cardiovascular diseases, such as atherosclerosis, diabetes, and hypertension. Up-regulation of a cytoprotective protein, heme oxygenase (HO)-1, is considered to augment the cellular defense against various agents that may induce cytotoxic injury. In the present study, we demonstrate that KRG water extract induces HO-1 expression in human umbilical vein endothelial cells (HUVECs) and possible involvement of the anti-oxidant transcription factor nuclear factor-eythroid 2-related factor 2 (Nrf2). KRG-induced HO-1 expression was examined by western blots, reverse transcriptase polymerase chain reaction and immunofluorescence staining. Specific silencing of Nrf2 genes with Nrf2-siRNA in HUVECs abolished HO-1 expression. In addition, the HO inhibitor zinc protoporphyrin blunted the preventive effect of KRG on $H_2O_2$-induced cell death, as demonstrated by terminal transferase dUTP nick end labeling assay. Taken together, these results suggest that KRG may exert a vasculoprotective effect through Nrf2-mediated HO-1 induction in human endothelial cell by inhibition of cell death.

• 대한한방부인과학회지
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• 제29권2호
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• pp.99-112
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• 2016

Objectives: Gyejibokryeong-hwan (GBH), a traditional Korean herbal medicine, has been widely used for the treatment of the blood stasis syndrome. This study is purposed to analyze the experimental research trend of GBH in Korea for developing further research plan. Methods: A search of Korean research database-Oasis, RISS and KISS- and Pubmed was carried out for publications until 2015, for the words, 'Guizhifulingwan', ‘Gyejibokryeonghwan’, or ‘Keishibukuryogan’. Then study selection is conducted according to PRISMA guidelines, studies not related or using modified formula or administered for human are excluded, 48 studies are included in this review, finally. We analyzed studies by research method, subject, outcome measure, and result of the study. Results: There were 31 in vivo studies about the effect of GBH on platelet aggregation, anti-oxidant, blood viscosity, and hypercholesterolemia, etc. 12 in vitro studies were about the effect of GBH on the cervical carcinoma, chronic kidney disease, uterine myoma, hepatocarcinoma, atherosclerosis, cancer chemo-prevent. 9 ex vivo studies were about the effect of GBH on the platelet aggregation, chronic kidney disease, ovaulatory disorder, and rheumarthritis.Conclusions: We proposed the translational research of GBH involving scientific discoveries and developing practical applications by investigating the concept of blood stasis syndrome in terms of current physiopathological mechanism.

• Journal of Animal Science and Technology
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• 제47권2호
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• pp.277-282
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• 2005

The current study was conducted to investigate the effect of organic selenium in mushroom cultured media (MCM) on beef quality. Each five of 20 Hanwoo steers(20 - 24 month, approximately 613 kg) were assigned to four levels of the selenium content(O.l, 0.3, 0.6, 0.9 dry matter based ppm), and the feeding treatments were enforced for 12 weeks. The results showed that m. longissimus from the 0.1 ppm feeding supplementation had greatly higher intramuscular fat content than other treatments(13.1 %). In meat color, the 0.1 ppm treatment showed the lowest lightness(CIE $L^*$)(P< 0.05) and had a tendency to have lower redness(CIE $a^*$). The treatment had no noticeable effect on moisture, protein, and ash content, cooking loss, water-holding capacity and purge loss. The 0.3 ppm treatment resulted in the toughest meat(assessed by WB-shear force) with 4.54 kg / inch', while other groups showed a similar toughness ranging from 3.3 to 3.7 kg/ inchl . Sensory characteristics in juiciness and flavor intensity had a tendency of increasing as organic selenium concentration increased, but tenderness was not influenced by the contents. The result indicated that the organic selenium feeding affects meat qualities to different extent, and further study is required to examine anti-oxidant effect of selenium in vivo.

• KSBB Journal
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• 제30권4호
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• pp.155-160
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• 2015

The present study was to investigate the antioxidant activity in ethanol and water extracts of root and stem of Sargassum coreanum. Antioxidant activities were evaluated by total polyphenol contents, DPPH radical scavenging activity, chelating effect, reducing power, and rancimat method. Total polyphenol contents of ethanol and water extracts were 32.79 mg/g and 15.55 mg/g, respectively. Ethanol extract showed higher DPPH radical scavenging activity than water extract and similar activity to BHT. Reducing power of extracts was increased in a concentration-dependent manner and ethanol extract had more reducing power than water extract. Ethanol and water extracts have little chelating effect at all concentrations. Antioxidant index (AI) of ethanol extract measured by Rancimat was higher than that of water extract, but their AI was lower than that of BHT. These results indicate that ethanol extract of S. coreanum root and stem has more potent antioxidant activity than water extract through DPPH radical scavenging and reducing power, and could potentially be used as a good source of natural antioxidants.

• 동의생리병리학회지
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• 제25권6호
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• pp.1008-1013
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• 2011

Osteoporosis is the leading underlying cause of fractures, particularly in postmenopausal women, due to the loss of estrogen-mediated suppression of bone resorption. More than 50% of adults 50 years of age or older are estimated to have osteoporosis. Osteoclast which is main target for treatment of osteoporosis is originated from hematopoietic cell line. Aloe has been widely used in worldwide country as a coadjuvant medicine. Extracts of the leaves of Aloe have been used in condition to improve dermatologic problem such as seborrheic dermatitis, aphthous stomatitis, xerosis, lichen planus and has been known to exert anti-inflammatory, anti-oxidant and anti-tumor effects. However, despite the popularity of aloe as a plant food supplements, the evaluation of its efficacy as a possible therapeutic option for osteoporosis remains scarce. Thus, we evaluated the effect of Aloe on receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation. Here we found that Aloe significantly inhibited osteoclast differentiation induced by RANKL. Aloe suppressed the activation of p38 pathway and $NF{\kappa}B$ in bone marrow macrophages (BMMs) treated with RANKL. Also, Aloe significantly inhibited the mRNA expression of c-Fos, tartrate-resistant acid phosphatase (TRAP), osteoclast-associated receptor (OSCAR), nuclear factor of activated T cells (NFAT)c1 and cathepsin K in BMMs treated with RANKL. Particularly, Aloe greatly inhibited the protein expression of c-fos and NFATc1. Taken together, our results suggested that Aloe may be useful tool for treatment of osteoporosis by inhibition of osteoclast differentiation.

• 한국식품과학회지
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• 제48권6호
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• pp.597-603
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• 2016

본 연구는 증숙 횟수에 따른 천마 추출물의 경구 투여가 산화방지 효과를 통한 위 점막 손상 억제에 미치는 효과를 평가하는 실험이다. 증숙 횟수를 달리한 천마 추출물을 준비하여 DPPH, ABTS 라디칼 소거능, total phenol, flavonoid, gastrodin, gastrodigenin 성분 분석 결과, 1회 증숙 천마와 9회 증숙 천마 추출물간의 급성 위염 개선 효능 평가가 가능할 것으로 판단되었다. HCl/ethanol로 유발된 급성 위염 동물 모델에 GE1과 GE9 (100 mg/kg body weight)과 sucralfate (10 mg/kg body weight)를 HCl/ethanol 처리 전 경구 투여하였다. 그리고 이를 정상군과 대조군과 비교 분석하였다. 급성 위염 개선 효능 실험에서 증숙 천마 추출물의 섭취는 HCl/ethanol로 유발된 위 점막 손상 마우스에서 위 조직의 육안적 손상을 감소시켰다. 9회 증숙 천마 추출물의 섭취는 위 점막 손상마우스에서 혈청의 ROS와 조직의 $ONOO^-$를 감소시켰고, 위 조직에서 염증성 매개인자인 $TNF-{\alpha}$ 또한 감소시켰다. 결론적으로 9회 증숙한 천마의 경구투여는 1회 증숙한 천마에 비하여 효과적으로 위 점막 손상을 억제하였다. 따라서 9회 증숙 천마 추출물의 투여가 급성 위염 유발 마우스 모델에서 위점막 손상 억제에 효과가 있다고 사료된다.

• Natural Product Sciences
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• 제26권1호
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• pp.83-89
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• 2020

Osteoporosis is a worldwide disease leading to significant economic and societal burdens globally. Osteoporosis is caused by unbalanced bone remodeling between the rate of osteoclast bone resorption and osteoblast bone formation. Acer tegmentosum Maxim (AT) is a traditional herbal medicine containing multiple biological activities such as anti-oxidant and anti-inflammatory purposes. However, its role in osteoporosis has not been fully studied. Therefore, we investigated whether AT has a potent inhibitory effect on osteoporosis and its mechanism through a systemic evaluation in ovariectomized (OVX) mice. OVX mice were orally administrated with the AT at doses of 50, 100, and 200 mg/kg for 10 weeks. Histological images and histomorphometry analyses were performed by H&E and Toluidine blue satin, and the expression levels of receptor activator for nuclear factor-kB ligand (RANKL), nuclear factor of activated T cells cytoplasm 1 (NFATc1), c-Fos, and matrix metalloproteinase 9 (MMP9) related to the osteoclast differentiation were investigated using immunohistochemical analysis. Administration of AT prevented bone loss and the alternations of osteoporotic bone parameters at the distinct regions of the distal femur and spongiosa region in OVX mice. Further, administration of AT increased periosteal bone formation in a dose-dependent manner. Meanwhile, AT inhibited not only the expression of NFATc1 and c-Fos, which are two major regulators of osteoclastogenesis but also reduced bone resorbed encoding expression of MMP9 and RANKL. Our results indicated that administration of AT prevented bone loss and the alternations of osteoporotic bone parameters at the distinct regions of the distal femur and spongiosa region in OVX mice. Also AT has the bone protective effect through the suppression of osteoclast and promotion of osteoblast, suggesting that it could be a preventive and therapeutic candidate for anti-osteoporosis.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제40권
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• pp.44.1-44.17
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• 2018

Background: Coffee extract has been investigated by many authors, and many minor components of coffee are known, such as polyphenols, diterpenes (kahweol and cafestol), melanoidins, and trigonelline, to have anti-inflammatory, anti-oxidant, anti-angiogenic, anticancer, chemoprotective, and hepatoprotective effects. Therefore, it is necessary to know its pharmacological effect on hepatocytes which show the most active cellular regeneration in body. Methods: In order to determine whether coffee extract has a beneficial effect on the liver, 20 C57BL/6J mice were intraperitoneally injected once with dialyzed coffee extract (DCE)-2.5 (equivalent to 2.5 cups of coffee a day in man), DCE-5, or DCE-10, or normal saline (control), and then followed by histological observation and IP-HPLC (immunoprecipitation high performance liquid chromatography) over 24 h. Results: Mice treated with DCE-2.5 or DCE-5 showed markedly hypertrophic hepatocytes with eosinophilic cytoplasms, while those treated with DCE-10 showed slightly hypertrophic hepatocytes, which were well aligned in hepatic cords with increased sinusoidal spaces. DCE induced the upregulations of cellular proliferation, growth factor/RAS signaling, cellular protection, p53-mediated apoptosis, angiogenesis, and antioxidant and protection-related proteins, and the downregulations of NFkB signaling proteins, inflammatory proteins, and oncogenic proteins in mouse livers. These protein expression changes induced by DCE were usually limited to the range ± 10%, suggesting murine hepatocytes were safely reactive to DCE within the threshold of physiological homeostasis. DCE-2.5 and DCE-5 induced relatively mild dose-dependent changes in protein expressions for cellular regeneration and de novo angiogenesis as compared with non-treated controls, whereas DCE-10 induced fluctuations in protein expressions. Conclusion: These observations suggested that DCE-2.5 and DCE-5 were safer and more beneficial to murine hepatocytes than DCE-10. It was also found that murine hepatocytes treated with DCE showed mild p53-mediated apoptosis, followed by cellular proliferation and growth devoid of fibrosis signaling (as determined by IP-HPLC), and subsequently progressed to rapid cellular regeneration and wound healing in the absence of any inflammatory reaction based on histologic observations.

• 생명과학회지
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• 제31권4호
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• pp.389-399
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• 2021

이 연구는 인간진피섬유아(HDF)세포에서 병풀 및 아시아티코사이드가 H₂O₂ 유래 세포주기 정지기, 미토콘드리아 활성 및 염증성 사이토카인에 미치는 영향을 조사하였다. 병풀 80% 메탄올 추출물, 에틸아세티이트 분획물 및 병풀의 대표물질인 아시아티코사이드를 사용하였다. 병풀 추추물, 에틸아세테이트 분획 및 아시아티코사이드로 처리한 세포는 낮은 수준의 TNF-α 및 IL-6을 분비하였고, 아시아티코사이드의 항산화 효과는 병풀 추출물 및 에틸아세테이트 분획물보다 높았다. 아시아티코사이드 처리는 미토콘드리아의 막포텐셜을 증가시키고, 미토콘드리아를 정상으로 되돌렸다. 스트레스 유도 후 에틸아세테이트 분획물 및 아시아티코사이드에 의해 미토콘드리아 산소 소비율이 증가하였고, TLR4-MyD88-TRAF6-p65 경로가 재감소하였다. 이러한 결과는 병풀 추출물, 에틸 아세테이트 분획 및 아시아티코사이드가 HDF 세포의 미토콘드리아 활성을 조절할 뿐 아니라 항산화 및 항염증에 효과 있음을 시사한다.

• 대한화장품학회지
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• 제44권1호
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• pp.39-48
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• 2018

본 연구에서는 커피생두의 로스팅 과정에서 쉽게 발견되는 커피 은피(coffee silver skin)의 열수와 에탄올 추출물을 사용하여 항산화, 미백, 주름개선, 세포독성실험을 진행하였다. 커피 은피의 열수와 에탄올 추출물은 농도가 증가함에 따라 농도 의존적으로 DPPH와 ABTS 라디칼 소거활성이 높아졌으며, 열수 추출물보다 에탄올 추출물이 항산화력이 높게 나타났다. 특히 에탄올 추출물의 경우 $50{\mu}g/mL$에서 92.26%의 ABTS 라디칼을 소거시켜 양성대조군과 비슷한 항산화 효과를 나타내었다. 미백효과의 정도를 조사하기 위하여 tyrosinase 저해효과와 DOPA 산화 저해효과를 조사한 결과 농도 의존적으로 저해효과를 나타내었다. 또한 주름개선효과의 정도를 조사하기 위해 elastase와 collagenase 저해 효과를 조사한 결과 elastase와 collagenase를 농도 의존적으로 저해 효과를 나타내었다. 각질형성세포(HaCaT)를 이용하여 세포독성 및 증식실험을 진행하였다. 커피 은피 추출물의 세포생존율은 무처리 대조군과 유사한 결과를 나타내었다. 그러므로 커피 은피는 주름개선, 미백, 항산화 효과를 나타내므로 화장품에 응용 가능한 기능성 소재로 평가된다.