• Korean Journal of Pharmacognosy
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• v.14 no.4
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• pp.149-160
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• 1983

It has been known that Gentianae macrophyllae Radix is reputed to be effective in removal of 'Pungseub' and 'Heoyeol', and to be analgesic. Since ancient times, however, there have been many examples of substituting Lycoctoni Radix for Gentianae macrophyllae Radix. For that reason, this experiment was conducted to compare the diuretic, analgesic, anti-inflammatory, anti-pyretic, anti-bacterial effect, and toxicity of Lycoctoni Radix with those of Gentianae macrophyllae Radix. The results obtained in this work were summarized as follows; In the test of acute toxicity, Lycoctoni Radix was found to be toxic in comparison to Gentianae macrophyllae Radix which was found to be non-toxic. Both herbs were found to be diuretic and Gentianae macrophyllae Radix was more effective than the other and to be ineffective for the blood pressure and respiration. Lycoctoni Radix was found to be more analgesic than the other. Gentianae macrophyllae Radix was found to be more anti-inflammatory of the two and both demonstrated anti-pyretic effect. Anti-bacterial effect was detected only in Lycoctoni Radix. It is concluded that Gentianae macrophyllae Radix has mild diuretic, analgesic, anti-inflammatory and anti-pyretic effect, but Lycoctoni Radix, due to its toxicity, can not be substituted for Gentianae macrophyllae Radix.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.19 no.1
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• pp.11-20
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• 2006

Objective : This experimental study was performed to investigate the effect of herbal eye drops, Sean-tang and Coptidis rhizoma on Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa keratitis. Methods : After administering herbal eye drops (Sean-tang, Coptidis rhizoma) on Staphylococcus ausreus, Staphylococcus. epidermidis and Pseudomonas aeruginosa, I measured MIC and the size of inhibition zone. MIC was measured by dropping to $50{\mu}{\ell}$ according to density(l00%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Result : On Staphylococcus aureus, Staphylococcus epidermidis, Coptidis rhizoma showed the highest anti-bacterial potency and on Pseudomonas aeruginosa, Sean-tang, Coptidis rhizoma was nor anti-bacterial potency. Conclusions : The present author think that the herbal eye drops, Sean-tang and Coptidis rhizoma can be used to cure Staphylococcus aureus, Staphyloccus epidermidis keratitis and if further study is performed, the use of the herbal eye drops will be valuable and beneficial in the clinical medicines.

• Ceramist
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• v.22 no.3
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• pp.301-315
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• 2019

We prepared hybrids between layered double hydroxide (LDH) and natural plant extract such as Peaonia suffruticosa Andrews (PS) and Peaonia Japonica (PJ) which was confirmed anti-bacterial activity through paper disc diffusion assay. According to X-ray diffractometer, scanning electron microscope, zeta-potential measurement and quantification of extract loading amount in hybrids, we confirmed that similar amount of PS and PJ loaded on inter-particle pore of LDH with partial adsorption on surface of LDH through reconstruction process. We also evaluated the bacterial colony forming inhibition of PS extract, PJ extract, PS-LDH and PJ-LDH hybrids against Escherichia coli as gram negative bacterium and Bacillus subtilis as gram positive bacterium, suggesting that both hybrids have enhanced anti-bacterial activity compared with extract itself.

• Journal of dental hygiene science
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• v.24 no.1
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• pp.54-61
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• 2024

Background: The Korean name for Angelica gigas Nakai (AGN) is Cham-dang-gui, which grows naturally or is cultivated, and its dried roots are used in traditional herbal medicines. The AGN root exert various pharmacological effects. Despite the various pharmacological effects of the AGN root, there are no reports on its anti-oral microbial effects. The purpose of this study was to reveal the anti-oral microbial effect and the microbial and biochemical changes in oral microorganisms according to the concentration of the ethanol extract of AGN (EAGN) root, and to confirm the possibility of using EAGN as a plant-derived functional substance for controlling oral infectious microorganisms. Methods: Disk diffusion test, growth measurement, biofilm formation assay, and measurements of acid production and buffering capacity were performed to confirm the antibacterial effect of EAGN. Results: EAGN showed anti-oral bacterial effects against Streptococcus mutans and Aggregatibacter actinomycetemcomitans at all concentrations, with S. mutans showing a more susceptible effect at concentrations above 5.0 mg/ml and A. actinomycetemcomitans at 3.75 mg/ml. EAGN treatment significantly reduced A. actinomycetemcomitans growth at all concentrations tested. Biofilm formation was significantly reduced at concentrations above 3.75 mg/ml for S. mutans and 2.5 mg/ml for A. actinomycetemcomitans. Acid production in S. mutans and A. actinomycetemcomitans was significantly increased by treatment with EAGN, and the buffering capacities of S. mutans and A. actinomycetemcomitans increased from an EAGN concentration of 3.75 mg/ml and above. Conclusion: EAGN showed anti-oral bacterial effects against both S. mutans and A. actinomycetemcomitans at concentrations above 3.75 mg/ml, which were thought to be related to the inhibition of their growth and biofilm formation. Therefore, EAGN can be used as a safe functional substance derived from medicinal plants owing to its antibacterial effects against S. mutans and A. actinomycetemcomitans.

• Biomedical Science Letters
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• v.26 no.4
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• pp.383-388
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• 2020

Ingestion of food contaminated with microorganism, if not always, could lead to severe health problem. Preservatives has been added to food to prevent food from being contaminated with microorganism. But, these have potential to threaten the health. Therefore, much effort has been taken to find the safe materials showing the anti-microbial activity. In this study, we investigated the anti-bacterial activity of Oenothera lamarckiana aerial part extract against eight bacteria strain. In paper disc assay, extract inhibited the growth of Staphylococcus aureus, Methicillin-resistant S. aureus, Bacillus cereus and Shigella dysenteriae at 200 μg/disc, but not against Escherichia coli, E. coli O157:H7, Salmonella Typhi and S. enteritidis. Minimum inhibitory concentration (MIC) against Staphylococcus aureus, Methicillin-resistant S. aureus, Bacillus cereus and Shigella dysenteriae is 250, 250, 500 and 500 μg/mL, respectively. Compared with reported MIC of other plant resources, O. lamarckiana aerial part extract showed the relatively high anti-bacterial activity. O. lamarckiana aerial part could be suitable for the preservative development. But, it still remains to be studied to evaluate safety and so on.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.25 no.4 s.34
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• pp.111-121
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• 1999

Acne vulgaris, the most common skin disease. can be formed as only a few comedons or severe inflammatory lesions. The pathogenesis of acne involves various factors; excessive androgen, excessive sebum production, abnormal alteration of follicular epithelium, proliferation of Propionibacterium acnes, and inflammation. We investigated acne therapy using oriental herbs described in the Korean traditional medical book(Dong-ui-bo-gam). Oriental herbs(Angelica daurica, Arctium lappa, Coptidis rhizoma, and Glycyrrhiza glabra) were chosen based on their respective property of sebum control, anti-inflammatory activity, and anti-bacterial activity. We examined the effect of acne treatment, in terms of chemotactic inhibition, lipogenesis inhibition, and anti-bacterial activity for P. acnes. 1. Neutrophil chemotaxis assay; P. acnes secrete chemotactic factors and other pro-inflammatory extracellular products. Neutrophil chemotactic activity of P. acnes was measured by 48-well chemotaxis method. Angelica daurica clearly suppressed chemotactic activity of P. acnes. 2. Using sebaceous gland of hamster ear lipogenesis assay; Sebaceous lipogenesis was measured using ear biopsies by incubation or $C^{14}$-acetate in culture media. The $C^{14}$-labeled lipids were extracted and determined by liquid scintilation counting. Coptidis rhizoma markedly inhibited sebum production. 3. Anti-bacterial assay for P. acnes(MIC test); Glycyrrhiza glabra showed anti-bacterial activity. P. acnes did not develop resistance against Glycyrrhiza glabra. Retinoids are effectively to inhibit sebum production and regulate follicular keratinization process, with little anti-inflammatory activity. Angelica daurica suppressed neutrophil chemotaxis, Coptidis rhizoma inhibited sebum production, and Glycyrrhiza glabra showed anti-bacterial activity against P. acnes. A combined formulation of Angelica daurica, Coptidis rhizoma. and Glycyrrhiza glabra is expected to provide effective acne treatment.

• Proceedings of the SCSK Conference
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• 1999.10a
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• pp.111-121
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• 1999

Acne vulgaris, the most common skin disease. can be formed as only a few comedons or severe inflammatory lesions. The pathogenesis of acne involves various factors; excessive androgen, excessive sebum production, abnormal alteration of follicular epithelium, proliferation of Propionibacterium acnes, and inflammation. We investigated acne therapy using oriental herbs described in the Korean traditional medical book (Dong-ui-bo-gam). Oriental herbs (Angleica daurica. Arctium lappa. Coptidis rhizoma, and glycyrrhiza glabra) were chosen based on their respective property of sebum control, anti-inflammatory activity, and anti-bacterial activity. We examined the effect of acne treatment, in terms of chemotactic inhibition, lipogenesis inhibition, and anti-bacterial activity for P. acnes. 1. Neutrophil chemotaxis assay ; P acnes secrete chemotactic factors and other pro-inflammatory extracellular products. Neutrophil chemotactic activity of P. acnes was measured by 48-well chemotaxis method. Angelica daurica clearly suppressed chemotactic activity of P. acens. 2. Using sebaceous gland of hamster ear lipogenesis assay; Sebaceous lipogenesis was measured using ear biopsies by incubation of $C^{14}$ -acetate in culture media. The $C^{14}$ -labeled lipids were extracted and determined by liquid scintilation counting, Coptidis rhizoma markedly inhibited sebum production, 3. Anti-bacterial assay for P. acnes (MIC test) Glycyrrhiza glabra showed anti-bacterial activity. P. acnes did not develop resistance against Glycyrrhiza glabra. Retinoids are effectively to inhibit sebum production and regulate follicular keratinization process, with little anti-inflammatory activity. Angelica daurica suppressed neutrophil chemotaxis. Coptidis rhizoma inhibited sebum production, and Glycyrrhiza glabra showed anti-bacterial activity against P. acnes. A combined formulation of Angelica daurica. Coptidisr hizoma and Glycyrrhiza glabra is expected to provide effective acne treatment.ent.ive acne treatment.

• Journal of Pharmacopuncture
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• v.10 no.1 s.22
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• pp.79-84
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• 2007

Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to 50${\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract was showed anti-bacterial potency compared with quantity and density in 100% and 10% of all samples(40g, 80g, 160g). 2. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples($20{\mu}l$, $30{\mu}l$, $40{\mu}l$, $50{\mu}l$) in 100% density, but anti-bacterial potency of 80g, $50{\mu}l$ Coptidis rhizoma extract decreased compared with 40g, $50{\mu}l$. 3. MIC on Staphylococcus epidermidis in 40g Coptidis rhizoma extract was showed in $50{\mu}l$, $40{\mu}l$ of 10% density, 80g Coptidis rhizoma extract was showed in $50{\mu}l$ of 10% density, 160g Coptidis rhizoma extract was showed in $50{\mu}l$, $40{\mu}l$, $30{\mu}l$ of 10% density. Conclusions : Coptidis rhizoma extract was showed anti-bacterial potency compare with quantity and density. In herbal drug, anti-bacterial potency compare with quantity and density must be studied.

• Journal of Animal Science and Technology
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• v.59 no.2
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• pp.4.1-4.5
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• 2017

Background: Biofilms were the third-dimensional structure in the solid surface of bacteria. Bacterial biofilms were difficult to control by host defenses and antibiotic therapies. Escherichia coli (E. coli) and Salmonella were popular pathogenic bacteria that live in human and animal intestines. Essential oils are aromatic oily liquids from plant materials and well known for their antibacterial activities. Method: This study was conducted to determine effect of essential oil on anti-biological biofilm formation of E. coli and Salmonella strains in in vitro experiment. Two kinds of bacterial strains were separated from 0.2 g pig feces. Bacterial strains were distributed in 24 plates per treatment and each plates as a replication. The sample was coated with a Bacterial biofilm formation was. Result: Photographic result, Escherichia coli (E. coli) and Salmonella bacteria colony surface were thick smooth surface in control. However, colony surface in blended and single essential oil treatment has shown crack surface layer compared with colony surfaces in control. Conclusion: In conclusion, this study could confirm that essential oils have some interesting effect on anti-biofilm formation of E. coli and Salmonella strains from pig feces.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.12
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• pp.5773-5778
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• 2011

Results regarding the anti-oxidative, anti-allergic, anti-bacterial and anti-cancer effects of lotus leaf and dandelion have been reported through active research on the medical effects of medicine prepared from crude drugs. This paper aimed to examine the anti-bacterial effect of lotus leaf and dandelion extracts on S. mutans by evaluating the colony forming unit and growth curve. In accordance with the growth inhibition rate of S. mutans according to the addition of lotus leaf and dandelion extracts, the higher the extract concentration was, the higher the growth inhibition rate of S. mutans was. This paper therefore verifies that lotus leaf and dandelion extracts have an effect in inhibiting the growth of S. mutans.