• 대한화장품학회지
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• 제31권1호
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• pp.103-109
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• 2005

자외선은 콜라겐 분해와 같은 피부 결합조직에서 특이적인 변화를 유발한다. 세포외 기질(extracelluar matrix)내에서의 많은 변형들은 기질 금속 단백질 분해효소(matrix metalloproteinases)에 의해 매개된다. 본 연구에서는 천연물 유래의 새로운 노화방지소재를 개발하기 위해 백렴 추출물의 용매별 분획들의 항산화 활성을 검색하고, 그 중에서 활성이 가장 높게 나타난 에틸아세테이트 층에 대해 MMP-1 활성 및 human dermal fibroblasts에서 자외선에 의한 MMP-1 발현에 미치는 영향을 측정하였다. 백렴 추출물의 에틸 아세테이트 층은 MMP-1의 활성을 농도 의존적으로 저해하였다($IC_{50}=9{\mu}g/mL$). 또한, 자외선에 의해 증가되는 MMP-1의 발현이 에틸 아세테이트 층을 $100{\mu}g/mL$ 처리한 경우 약 $90\%$ 정도 저해되었다. 반면에 MMP-1 mRNA의 발현에는 별다른 영향을 미치지 않는 것으로 나타났다. 따라서 백렴 에틸 아세테이트 층은 MMP-1의 발현을 단백질 수준에서만 저해함을 알 수 있다. 결론적으로 백렴 에틸 아세테이트 층은 자외선에 의해 손상된 피부를 보호할 수 있는 새로운 노화방지 소재로 이용될 수 있다.

• 생명과학회지
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• 제33권4호
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• pp.349-356
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• 2023

만성 염증성 관절염 질환인 통풍은 고요산혈증을 특징으로 하며 NLRP3 inflammasome의 활성화에 따른 IL-1β와 같은 염증성 cytokine 방출과 연관된 MSU에 대한 염증 반응에 의해 유발될 수 있다. 마늘에 함유된 황 함유 화합물은 다양한 질병에 대한 잠재적인 유익한 약리학적 효능을 가지지만, NLRP3 inflammasome 활성화와 연관된 통풍 억제에 대한 효능은 현재까지 입증되지 않았다. 본 연구에서는 대표적인 마늘 유래 황화합물인 DADS와 DATS가 MSU에 의한 NLRP3 inflammasome 활성을 억제할 수 있는지를 조사하였다. 본 연구의 결과에 의하면, 비세포 독성 조건에서 DADS와 DATS는 LPS가 전처리된 RAW 264.7 대식세포에서 MSU에 대한 반응으로 증가된 NO의 생성과 IL-1β 유리를 유의적으로 차단하였다. DADS와 DATS는 또한 증가된 NLRP3, ASC, caspase-1 p20 및 IL-1β의 발현을 감소시켰으며, 이는 MSU로 유도된 LRP3 inflammasome 활성화가 DADS와 DATS에 의해 억제되었음을 의미한다. 아울러, DADS와 DATS는 NLRP3 inflammasome 활성화에 상위 신호로 작용하는 산화적 스트레스를 차단했으며 ROS 생성을 제거한다는 사실에서 입증되었다. 결론적으로, 본 연구의 결과는 DADS와 DATS가 ROS/NLRP3 경로를 억제하여 inflammasome 활성화를 차단함으로서 NLRP3 의존성 통풍성 관절염 치료를 위한 잠재력이 우수함을 의미한다.

• 한국축산식품학회지
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• 제40권1호
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• pp.106-117
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• 2020

Cellular senescence is associated with age-related vascular disorders and has been implicated in vascular dysfunctions. Here, we show that duck oil-loaded nanoemulsion (DO-NE) attenuates premature senescence of vascular smooth muscle cells (VSMCs) triggered by angiotensin II (Ang II). Compared with control nanoemulsion (NE), DO-NE significantly inhibited the activity of senescence-associated β-galactosidase, which is a biomarker of cellular senescence, in Ang II-treated VSMCs. SIRT1 protein expression was dose- and time-dependently induced in VSMCs exposed to DO-NE, but not in those exposed to NE, and SIRT1 promoter activity was also elevated. Consistently, DO-NE also dose-dependently rescued Ang II-induced repression of SIRT1 expression, indicating that SIRT1 is linked to the anti-senescence action of DO-NE in VSMCs treated with Ang II. Furthermore, the SIRT1 agonist resveratrol potentiated the effects of DO-NE on VSMCs exposed to Ang II, whereas the SIRT1 inhibitor sirtinol elicited the opposite effect. These findings indicate that DO-NE inhibits senescence by upregulating SIRT1 and thereby impedes vascular aging triggered by Ang II.

• Toxicological Research
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• 제4권1호
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• pp.13-22
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• 1988

The inhibitory effect of three polyacetylene compounds, panaxydol, panaxynol and panaxytriol isolated from Panax ginseng C.A. Meyer on $CCl_4$induced lipid peroxidation in vivo and in vitro hepatic microsomal lipid peroxidation induced by ADP-$Fe^{3+}$, NADPH and NADPH-cytochrome P-450 reductase were investigated. Their effects on lowering the lipid peroxide levels both in serum and liver and lowering the serum enzyme (GOT, GPT, LDH) activities without the $CCl_4$-induction were also determined. Male ICR mice were pretreated i.p. with polyacetylene compounds or DL-${\alpha}$-tocopherol before administration of $CCl_4$ i.p. and 20 hr after the administration of $CCl_4,$ serum and liver were analyzed. Hepatic microsome was isolated and used for the in vitro NADPH-dependent lipid peroxidation system. Except for panaxynol, treatment with polyacetylenes to control mice did not reduce the levels of lipid peroxides and serum enzyme activities. Panaxynol itself inhibited lipid peroxidation in the liver of normal mice. Polyacetylene compounds protected from the $CCl_4$-induced hepatic lipid peroxidation and lowered serum lipid peroxide levels. Polyacetylenes also inhibited the in virto hepatic microsomal lipid peroxidation in a dose-dependent manner. The results suggest that panaxydol, panaxynol and panaxytriol seem to be the antioxidant components which contribute the anti-aging activities of Panax ginseng C.A. Meyer.

• Journal of Acupuncture Research
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• 제23권3호
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• pp.117-127
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• 2006

Alzheimer's disease (AD) is the most prevalent form of neurodegenerative disease associated with aging in the human population. This disease is characterized by the following 4 structural changes : Atrophy of the Cortex, Parasympathetic, and other neural cells, the existence of Neurofibrillary tangles (NFTs), and the accumulation of Senile plaques. NFTs and Senile plaques is known to be the index of this disease. Senile plaques disturbs the neutro transmission and depletes of Acetylcholine. So, Recovery of Acetylcholine is the primal objective for treating Alzheimer's disease. So, Inhibiting the activity of Acetylcholine Esterase (AChE), which causes the hydrolysus of acetylcholine into choline and acetate, can be seen as a key role for treating Alzheimer's disease. Increasing body of evidence has been demonstrated that Bee Venom Acupuncture (BV) could compete with complex protein involving in multiple step of $NF-_{\kappa}B$ activation and exert the anti-inflammatory potential of combined inhibition of the prostanoid and nitric oxide synthesis systems by inhibition of IKK and $NF-_{\kappa}B$. BV dose-dependently attenuated Scopolamine-induced Acetylcholine esterase activities in cerebral cortex and hippocampus of the mice brain. This study therefore suggests that BV acupuncture method may be useful for prevention of development or progression of AD.

• 대한화장품학회지
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• 제23권2호
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• pp.7-22
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• 1997

Inhibitory effects of 200 medicinal plants on elastase activity were screened. Among the 200 plants, six plant extracts exhibited more than 65% of inhibition on elastase activity by their total methanol extracts at 1, 000$\mu\textrm{g}$/$m\ell$ as a final concentration. For six active plants including Areca catechu, Cinnamonum cassia, Myristica frograns, Curcumma longa, Alponia katsumadai, and Dryopteris crassirrhizoma, the inhibitory effects of their comsituents were examined on the activity of human leukocyte elastase, hyaluronidase and lipid peroxidation. In lipid peroxidation assay using TBA method, three of the 6 plants including Curcumma longa, Areca Catechu and Alponia katsumadai exhibited more than 70% of inhibition at the concentration of 1, 000$\mu\textrm{g}$/$m\ell$, but only one plant, Areca Ctechu showed high inhibitory effect on hyaluronidase activity. The results suggest that medicinal plants showing several biological activities may be potent inhibitors of anti-aging process on skin and that might be useful for application in cosmetics.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1996년도 추계학술대회 논문집
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• pp.117-120
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• 1996

In this paper, It is tested by spraying the spoiled liquids on the materials to prove the process of tracking under the various environments. Our experiments ware examined by setting the materials in the spray chamber, and by spraying the distilled water(DW), the city water(CW), the underground water(UW), the artificial acid rain(AR) and the salt water(SW) in applied voltage 3-6[KV]. As the result, the time of tracking breakdown became shorter in the order of DW < CW < UW < AR < SW. XLPE was eroded and its anti-bracking property was very strong, but that of PVC sheath was week. Especially it was confirmed that spraying by AR and SW on the PVC sheath had a serious effect on the lifetime of cable by the tracking breakdown.

• 한국의상디자인학회지
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• 제19권3호
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• pp.63-74
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• 2017

This study aims to contribute to the development of male incontinence underwear with an anti-aging effect that meets customer requirements by examining the types, sorts, sizes and specifications of male incontinence underwear currently sold on the market. The results of an analysis of a total of eight commercial products are as follows. 1) Among the samples, six types of incontinence underwear were of a pull-on style like regular underwear; two types were of a pad insertion style. With respect to the underwear design, four (50%) types of incontinence underwear were brief style. The rest were drawers (3, 37.5%). Only one was of a trunk (12.5%) style. 2) With respect to size, the percentage of Size 3 was 50%, followed by Size 4 (50%). Most of the size of the underwear uses hip measurement whereas Japanese products (3, 37.5 %) use waist circumference. 3) With respect to the materials of the incontinence underwear, natural fibers such as cotton were mostly used and a polyurethane or polyester was mixed to enhance the wearing sensation and stretchability of the underwear. Finally, for the structure of the pad area, a napping finish was the largest (3, 37.5%), single knit 2(25%), twill weave 2(25%), and rib weave 1.

• 대한영양사협회학술지
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• 제10권1호
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• pp.58-64
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• 2004

This study was performed to investigate the kinds of health food advertised and sold through media like printed matter, TV broadcasting or internet. It was found that fifty three percent of the health food was circulated through internet, 27% through printed matter, 9% through TV Broadcasting. When the health foods was classified, the proportion of special purpose nutritious foods was 36.1%, health aid foods 33.9%, uncooked powdered foods 18.5%, functional foods for health 4.9%, Ginseng products 3.9%, and tea 0.9%. The special purpose nutritious foods was composed of 59% of nutrition supplementary foods, 24% of dietary fiber foods, 9% of weaning foods, 7% of milk formula and 2% of HCA. Vegetable extracts occupied 21.1% of health aid foods, yeast 7.5%, mushroom extracts 7.0%, chitosan 6.2%, aloe 5.3%. Dried powdered type health food occupied the highest proportion. The other type were capsule(18.8%), tablet(18.1%), and liquid type(16.4%). When the health food was classified with health claim, the proportion of "nutrition supplementation" was the highest (23.9%), "diet" 14.9%, "ergogenic"(18.8%), "promoting bowel movement" 7.8%, "growth" 5.7%, "regulation of blood sugar" 4.5%, "improving of immunity"(2.4%) and anti-aging effect(2.4%).

• 한국잠사곤충학회지
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• 제41권3호
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• pp.216-221
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• 1999

This study was designed to investigate the effects of silk fibroin powder on oxygen radicals and their scavenger enzymes in serum of rats. Sprague-Dawley(SD) male rats (160${\pm}$10 g) were fed experimental diets (SFP-2.5 and SFP-5.0 groups)added 2.5 and 5.0 g/kg BW/day for 6 weeks. Triglyceride levels were remarkably inhibited (15∼25%) in SFP-2.5 and SFP-5.0 groups compared with control group. Hydroxyl radical ($.$OH) formations resulted in a marked decrease (15%∼25%) compared with control group, while superoxide radical and hydrogen peroxide formations resulted in a significant decrease (6∼12% and 10%). Lipid peroxide and oxidized protein (>C=O group) productions resulted in a significant decrease (6∼12% and 6%) compared with control group. Superoxide dismutase (SOD) and catalase (CAT) activities were remarkably increased (35∼55% and 40∼50%), but glutathione peroxidase (GSHPX) activities were significantly increased (13%) compared with control group. These results suggest that anti-aging effect of silk fibroin powder (SFP) may play a pivotal role in attenuating a various age-related changes.