• 대한한방내과학회지
• /
• 제28권4호
• /
• pp.671-680
• /
• 2007

Objectives : The purpose of this study was to examine the effect of Haeyeol-tang on infectious disease of respiratory system. Methods : Haeyeol-tang was used by cold storage after dilution with 1, 10 and $100{\mu}g/ml$ of PBS, from dried material as extracted material with extraction with 50% ethanol. Statistical significance was examined through measuring TNF-$\alpha$, RT-PCR, DPPH, nitric oxide(NO), and MTT of Raw264.7 cells activated with LPS. Results : For the toxicity experiment of Haeyeol-tang, almost no toxicity was shown on cells in the concentration ranges of 1, 10 and $100{\mu}g/ml$. For the effect of NO production, Haeyeol-tang showed significant (p<0.001) decrease of NO production, according to its concentration. For the effect on the eliminating activity of DPPH radical, Haeyeol-tang showed increasing tendency of eliminating activity of DPPH radical, according to its concentration. For the effect on revelation of NOS2 enzyme, Haeyeol-tang showed inhibiting tendency against the revelation of NOS2, according to its concentration. For the effect on the TNF-$\alpha$ production, Haeyeol-tang showed significant (p<0.001) decrease of TNF-$\alpha$ production. Conclusions : This result means that Haeyeol-tang is effective for anti-inflammation, as well as excellent effect for anti-oxidation and anti-aging.

• 한국식품과학회지
• /
• 제17권6호
• /
• pp.506-515
• /
• 1985

홍삼(紅蔘)사포닌 가수분해물(加水分解物)의 항산화작용(抗酸化作用)에 의한 노화억제작용(老化抑制作用)을 구명(究明)하기 위하여 홍삼(紅蔘)사포닌을 가수분해(加水分解)하여 얻은 prosapogenin, panaxadiol 및 panaxatriol을 시료(試料)로 하여 in vitro 및 in vivo 실험(實驗)을 통하여 이들 가수분해물(加水分解物)들의 생체(生體) 과산화지질생성(過酸化脂質生成)에 미치는 억제효과(抑制效果)를 비교(比較)하였다. DPPH에 대한 전자공여능(電子供與能)(EDA)은 $13.88{\sim}19.76%$를 나타내고 있었으며 in vitro에서 과산화지질생성(過酸化脂質生成) 억제효과(抑制效果)는 현저하였으며 그 순서는 prosapogenin>panaxatriol>panacadiol이었다. POV에 의한 유효기간(誘導期間)은 이들 가수분해물(加水分解物) 홍삼(紅蔘)사포닌보다 훨씬 높았으며 prosaposenin>panaxatriol>panaxadiol의 순이었다. in vivo에서 복강(復腔)(i. p.) 및 경구(經口)(p.o.) 투여(投與)에 의한 생체내(生體內) 과산화지질생성(過酸化脂質生成) 억제효과(抑制效果)는 대조군(對照群)에 비(比)해 훨씬 효과적(效果的)이었으며, 홍삼(紅蔘)사포닌과 비교(比較)했을 때 간장(肝臟)과 신장(腎臟)에는 거의 비슷한 효과(效果)를 나타냈지만 혈액(血液)에서는 이들 가수분해물(加水分解物)이 홍삼(紅蔘)사포닌보다 훨씬 효과적(效果的)이었다. 효소활성(酵素活性)으로서 superoxide dismutase 활성(活性)을 in vitro에서 비교해 보면 이들 가수분해물(加水分解物)이 $24.2{\sim}36.4%$의 과산화지질생성(過酸化脂質生成) 억제효과(抑制效果)를 나타내고 있어, 홍삼(紅蔘)사포닌의 12.1%보다 $2{\sim}3$ 배(倍)의 효과(效果)를 나타내고 있었고, in vivo에서는 복강(復腔)(i. p.) 투여(投與)에서는 $12.9{\sim}22.2%$. 경구(經口)(p.o.) 투여(投與)에서는 $11.2{\sim}21.6%$의 높은 활성(活性)을 나타내고 있었다. 단 peroxidase 활성(活性)은 복강(復腔)(i. p.) 투여(投與)에서는 $129.0{\sim}188.6%$, 경구(經口)(p. o.) 투여(投與)에서는 $111.4{\sim}139.6%$의 활성(活性)을 나타내고 있었다.

• 한국식품영양학회지
• /
• 제28권6호
• /
• pp.941-946
• /
• 2015

본 연구에서는 HA에 대하여 UVB로 주름을 유도시킨 hairless mice에게 경구 투여를 하였을 때, 주름 개선에 미치는 영향을 관찰하였다. 쥐는 UV를 쪼여 광노화를 진행하였으며, HA는 UVB에 노출시키기 4주 전부터 투여하여 총 14주 동안 투여하였다. 주름 개선 평가는 replica 제작하여 주름의 길이, 깊이, 개수, 두께로 평가하였다. 또한, 피부의 조직학적 평가를 통해 진피층 내 교원섬유와 탄력섬유의 양과 형태를 관찰하였다. 10주 동안의 HA를 급여하였을 때 주름의 깊이, 두께, 개수를 측정한 주름 개선 평가에서 세 항목 모두 개선되는 결과를 보였다. 특히 HA의 160 mg/kg 농도에서는 주름의 깊이는 $19.44{\pm}0.75{\mu}m$, 주름 수는 $654.00{\pm}98.34$, 주름두께는 $1.35{\pm}0.08mm$로 양성 대조군(RA)의 결과(주름깊이; $19.28{\pm}0.95{\mu}m$, 주름 수; $953.57{\pm}83.54$, 주름두께; $1.34{\pm}0.07mm$)와 비슷한 수치를 나타내며, 우수한 피부 광노화 개선효과를 보였다. 하지만, 주름길이의 결과에서는 유의적 차이를 나타내지 않았다. HA의 섭취로 인한 교원섬유 및 탄력섬유 염색을 통한 조직학적 평가에서는 UV에 의해 밀도가 엉성하고 배열이 불규칙적이던 섬유들이 개선된 것을 관찰할 수 있었다. 이상의 결과로 보아 HA를 경구 투여 시 UV로부터 유도된 광노화 억제 및 피부주름 개선에 효능이 있음을 확인하였다.

• Molecular & Cellular Toxicology
• /
• 제4권2호
• /
• pp.150-156
• /
• 2008

The photoprotective effects of minerals from Korean indigenous ores, consisting mainly of sericite, on UVA-irradiated human dermal fibroblast (HDF) were examined. Zymographic analysis showed that the treatment of the minerals significantly reduced the UVA-enhanced MMP-1 activity and mRNA level. The minerals also showed strong inhibitory effect on MMP-2 activity and mRNA expression. Moreover, the minerals were better than polyphenol in reducing MMP-1 and MMP-2 expressions. Notably, the minerals significantly enhanced collagen biosynthesis in the HDF. Inhibition of the elastase activity and protection against the oxidatively damaged HDF cell were also found in the presence of the minerals. Taken together, the ore minerals may be used as the potent photo-protective and anti-skin-aging ingredients which can prevent skin cell damage by UVA.

• 한방비만학회지
• /
• 제7권2호
• /
• pp.39-44
• /
• 2007

Objectives In recent years, we are concerned about anti-aging and longevity. And I heard that Mori Fructus has been used for promotion of health in local clinics. So I wanted to know immuno-ability of Mori Fructus and carried out this experiment. Methods We cultivated 3T3-L1 Preadipocytes and Protein chip used for $ProteoPlex^{TM}$ 16-Well Murine Cytokine Array Kit. Results We known the immunity of Mori Fructus about 3T3-L1 Preadipocytes and gained the increase of Cytokines(IL-2, IL-4, IL-12p70, GM-CSF, INF-${\gamma}$, TNF-${\alpha}$). Conclusions So I guess that Mori Fructus has effect of immuno-ability, etc.

• Journal of Platform Technology
• /
• 제10권3호
• /
• pp.3-10
• /
• 2022

The effectiveness of an innovative skin treatment system that delivers an anti-aging solution deep into the skin without invasiveness and pain using a non-invasive air technology was investigated. In addition, an effective change using a non-invasive technique for delivering a solution for skin improvement was confirmed. The equipment named Cellre Jet is an effective skin care and drug delivery equipment that instantly opens the skin epidermis by using a maximum output pressure of 6 bars and high-pressure purified oxygen of 75-90% purity to deliver various nano-sized vital substances deep into the skin, and it uses the method of precisely controlling the equipment through an 8-inch digital touch display to accurately dispense the prescribed dosage. In this study, changes in skin condition were analyzed using this equipment and nano ampoules on subjects with actual skin problems through a related comparison and effectiveness judgment program. Through this study, skin care and drug delivery are possible, which will contribute to verifying the effectiveness of this non-invasive drug delivery equipment in the future, and is expected to establish the systematic effect in observing and studying changes in the skin.

• 대한화장품학회지
• /
• 제42권2호
• /
• pp.163-171
• /
• 2016

스테로이드 호르몬인 glucocorticoid (GC)는 glucocorticoid receptor (GR)와 결합하여 염증 유전자의 발현을 억제함으로써 강력한 항염 효과를 준다. 따라서 GR을 활성화하는 GC 제제들이 개발되어 피부염의 치료제로 사용되어 왔다. 그러나 이러한 GC 제제들은 피부 장벽 기능 저하, 진피층 두께 감소 등의 부작용을 유발하여 피부를 손상시키고 피부 노화를 유발한다. 특히 GC 성분은 11 beta-hydroxysteroid dehydrogenase type 1 ($11{\beta}$-HSD1)에 의해 활성화되어 GR의 활성을 높이는 것으로 보고되어 있다. 이에 본 연구에서는 스테로이드 제제인 dexamethasone에 의해 증가된 $11{\beta}$-HSD1의 발현을 효과적으로 억제할 수 있는 천연 소재를 발굴하고자 하여, 초두구 추출물에서 유래한 alpinetin에서 유의한 효과가 있음을 확인하였다. Alpinetin은 진피 섬유아세포에서 dexamethasone에 의해 발현이 증가한 $11{\beta}$-HSD1를 억제함과 동시에 GR의 활성 및 cortisol의 생성을 감소시켰다. 또한 사람 섬유아세포 및 3D skin model을 이용한 평가에서, alpinetin은 dexamethasone에 의한 콜라겐 감소와 진피층 두께 감소를 효과적으로 회복시켰다. 따라서, 본 연구 결과로부터 alpinetin은 $11{\beta}$-HSD1의 발현 증가에 의한 피부 스트레스 및 피부 노화를 효과적으로 예방할 수 있을 것으로 사료되었다.

• Natural Product Sciences
• /
• 제21권2호
• /
• pp.128-133
• /
• 2015

Lindera obtusiloba has been widely used as a traditional medicine for the treatment of lots of diseases, including abdominal pain, bruise, and hepatocirrhosis. Here in this study, we elucidated the lifespan-extending effect of methanolic extract of Lindera obtusiloba (MLO) using Caenorhabditis elegans model system. We found that MLO has potent lifespan extension activities under normal culture condition. Then, we determined the protective effects of MLO on the stress conditions such as osmotic, thermal and oxidative stress. To reveal possible mechanism of MLO-mediated lifespan, we further investigated the effect of MLO on the antioxidant enzyme activities and intracellular ROS levels. Our results demonstrated that superoxide dismutase and catalase activities were significantly up-regulated by MLO treatment, resulted in reduced intracellular ROS levels. In this work, we also tested whether MLO-mediated longevity activity was associated with aging-related factors such as food intake and growth. Our data revealed that both of pharyngeal pumping rate and body length were significantly shifted by MLO treatment, indicating these factors were involved in MLO's lifespan-extension effects. Although MLO induces reduction in food intake, the body movement of MLO-fed aged worms was not decreased, compared to untreated control worms, indicating MLO might extend lifespan without affecting healthspan.

• Journal of the Korean Wood Science and Technology
• /
• 제42권1호
• /
• pp.34-40
• /
• 2014

흰개미 방제 및 방곰팡이 효력이 입증된 저독성 네오티코치드계 목재보존제가 집성재 제조용 레조르시놀계 접착제의 접착력에 미치는 영향을 조사하였다. ACQ, CUAZ, 네오니코치드계 약제를 도포 처리한 다음 건조시킨 소나무 판재를 사용하였으며, 집성 접착 후에 블록 전단시험편을 채취하여 상태 및 촉진 열화 시험 후의 전단접착력과 목파율을 측정하여 비교하였다. ACQ 및 CUAZ 처리 집성재는 무처리 및 네오니코치드계 목재보존제 처리 집성재에 비하여 낮은 전단접착력을 보여 접착력 저하가 인정되었다. 이것은 이들 방부제 중에 함유되어 있는 구리화합물에 기인하는 것으로 판단되었다. 네오니코치드계 목재보존제 처리 집성재는 상태 및 촉진열화 시험 후에도 가장 우수한 전단접착력을 나타내 레조르시놀계 접착제의 접착성능에는 영향을 미치지 않는 것으로 판단하였다.

• Journal of Ginseng Research
• /
• 제35권3호
• /
• pp.272-282
• /
• 2011

Ginseng (Panax ginseng Meyer) has been shown to have anti-aging effects in animal and clinical studies. However, the molecular mechanisms by which ginseng exerts these effects remain unknown. Here, the anti-aging effect of Korean red ginseng (KRG) in rat testes was examined by system biology analysis. KRG water extract prepared in feed pellets was administered orally into 12 month old rats for 4 months, and gene expression in testes was determined by microarray analysis. Microarray analysis identified 33 genes that significantly changed. Compared to the 2 month old young rats, 13 genes (Rps9, Cyp11a1, RT1-A2, LOC365778, Sv2b, RGD1565959, RGD1304748, etc.) were up-regulated and 20 genes (RT1-Db1, Cldn5, Svs5, Degs1, Vdac3, Hbb, LOC684355, Svs5, Tmem97, Orai1, Insl3, LOC497959, etc.) were down-regulated by KRG in the older rats. Ingenuity Pathway Analysis of untreated aged rats versus aged rats treated with KRG showed that the affected most was Cyp11a1, responsible for C21-steroid hormone metabolism, and the top molecular and cellular functions are organ morphology and reproductive system development and function. When genes in young rat were compared with those in the aged rat, sperm capacitation related genes were down-regulated in the old rat. However, when genes in the old rat were compared with those in the old rat treated with KRG, KRG treatment up-regulated C21-steroid hormone metabolism. Taken together, Cyp11a1 expression is decreased in the aged rat, however, it is up-regulated by KRG suggesting that KRG seems enhance testes function via Cyp11a1.