• Journal of Plant Biotechnology
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• v.31 no.1
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• pp.7-11
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• 2004

This study was carried out to establish a new breeding scheme which is connected with conventional breeding method and anther culture method. To develop a herbicide resistant and direct seeding rice, $F_1$ plants were subjected to anther culture and regenerated plants from 5 crosses were studied to confirm the introduction of bar gene. After PCR analysis, we selected 227 plants which were carrying herbicide resistance gene (bar) out of 1,508 regenerated plants from anther culture. Among 169 $A_2$ lines carrying herbicide resistant gene from 5 crosses including YR23235 (Dongjin Ds3(Ba $r^{R}$)/ Milyang165), 42 lines that had superior agronomic characters were selected for further research. Among them, YR23235Acp79 which showed herbicide resistance, direct seeding adaptability and superiority in major agronomic characters was named Milyang 204. This breeding scheme proved that the anther culture of $F_1$ plants crossed between transformant and cultivar or transform ant alone could be utilized in breeding programs for a rapid progeny fixation and development of a variety.y.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.4
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• pp.328-333
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• 1984

Variation in ploidy level of regenerated plants from rice anthers and effective diploidization methods of haploid plants were studied to obtain basic information in rice breeding through anther culture. In a total of 574 plants derived from anther culture using 14F$_1$ hybrids as materials, there were 49.7% haploids, 48.6% diploids and 1.7% polyploids, respectively. The frequency of haploids in Japonica/Indica crosses was 60.6%, and that of Japonica/Japonica crosses was 43.0% in average. Inclusion of 2.4-D or NAA as phytohormone may increase the frequency of haploids, but kinetin may increase the frequency of diploids. The rate of auto-diploidization by tiller separation of haploid plants showed 8.2% in average. The rate of diploidization by leaf-sheath injection of colchicine showed 18.8% in average. Morphological characters of haploids plants showed that 64.6% in culm length, 63.4% in panicle length, 68% in flag leaf length, and 74.4% in flag leaf width compared to diploid plants. These apparent morphological differences will contribute to identify the ploidy of plants derived from rice anther culture.

• Korean Journal of Plant Tissue Culture
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• v.21 no.5
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• pp.293-297
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• 1994

In order to induce immature pollen derived plants, anthers of Ranunculus japonicus Thunb. were cultured on Murashige and Skoog's medium supplemented with various combinations of auxins and cytokinins. The combinations of NAA and BA were more effective than those of 2,4-D and kinetin in the formation of calli and embryos. Up to 5t5% of the anthers cultured on medium containing 0.5 mg/L NAA and 1.0 mg/L BA gave rise to plantlets. The most suitable stage for anther culture in the induction of calli and/or embryos from immature pollens was at the uninucleate and early binucleate stage (3 days before anthesis). Immature pollens developed into embryos by repeated division of the vegetative nucleate after 60days of culture.

• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.251-256
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• 1998

The effect of several factors on efficiency of anther culture of broccoli was studied. The medium supplemented with 14% sucrose, 125mg/L of silver nitrate and 0.1mg/L of each of NAA and 2,4-D was best in embryogenesis in all varieties tested. The effect of auxin was affected by silver nitrate, that is, it lowered embryogenesis when the concentration of NAA and 2,4-D was low, but it enhanced when their concentrations were high. Benzyl aminopurine increased embryogenesis in some varieties, but decreased in others. Although microspore embryogenesis was observed in anthers of which heigest was 1 to 0.6 - 1.0 of petal heigest in the bud, which corresponds to the early uninucleate to early binucleate stage of the microspores, it was much better when the ratio of heigest was 1 : 0.8. More embryos ware inducted from culture in March than in February in general. Because there was a big yearly variation, it was assumed that microspore embryogenesis was influenced greatly by the culture condition and the physiological condition of donor plants.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.3
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• pp.271-276
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• 1985

Effects of media and its components on callus induction and plant regeneration were studied to increase the cultural efficiency in rice anther culture. The N$_{6}$ basic medium gave better results in callus induction than those of MS or Miller. The medium used for callus induction affects the plant regeneration. The frequency of plant regeneration from callus grown on Miller basic medium was lower than those of N$_{6}$ or MS. Most of calli derived from anthers, above 90%, were induced from 20 days to 40 days after anther inoculation. The cultural efficiency of modified N$_{6}$ basic medium which was composed of 31.5mM KNO$_3$ and 1.75mM(NH$_4$)$_2$SO$_4$ as nitrogen sources was higher than those of N$_{6}$ basic medium. Combination of NAA and Kinetin showed better results than that of 2, 4-D only in cultural efficiency. Effect of DL-alanine on callus induction in Indica variety, IR40, showed better response in the anthers pretreated for 6 days at 12$^{\circ}C$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.207-210
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• 2000

We investigated the structure of floral organs and possibility of seed-set to breed a variety in ginger Zingiber officinale Rosc. Floral bud was formed from collected domestic Seosan var, and foregin Thailand var, the number of florets per bud were 8 and 10 in Seosan and Thailand var, respectively, Flowering time ranged from 18 to 25 August irregularly at 4-5 pm. The flower has the long styled with fiber hairs on top of stigma and connected-two anthers. Pollens were mixed of circular and ellips shape and its extine was two layer structure. Callus formation from anther explants was effective with compact and embryogenic on N$_{6}$ medium supplemented 2 mg/l of NAA(NCM). Plant regeneration was on the MS medium with BA of 1-2 mg/l from 40 days old callus after transferred callus medium.m.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.1
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• pp.74-78
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• 1991

This experiment was conducted to know the effects of mutagen treatments on callus induction, plant regeneration and their ploidy in the anther culture of wheat. The winter wheat cultivars, 'apos;and 'apos;Wonkwang'apos;, were treated at the mid or late-uninucreate stage under 4 different doses (100, 200, 500 and 1,000 rad.) of X-ray and 3 different levels(0.1, 0.2 and 0.3 mole) of Ethyl Methane Sulphonate. The anthers treated were set on the C$\_$17/medium for callus induction, and callus induced was transfered to 1/2 MS medium for plant regeneration. The mutagen treatments inhibited the callus induction but increased the plant regeneration in the callus which were induced from the anther set on the medium for the long time of 60 to 80 days. Also, the chromosome number to the regenerated plant varied largely by increasing of haploid plants(n=3x=21) and by occurring of aneuploidy having n=20 and n=22 of chromosome number.aried largely by increasing of haploid plants(n=3x=21) and by occurring of aneuploidy having n=20 and n=22 of chromosome number.

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.378-379
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• 2000

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.358-359
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• 2000

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.108-109
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• 2000