• Journal of Korean Society on Water Environment
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• v.20 no.5
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• pp.480-487
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• 2004

This study was conducted to find the effect of electron acceptors on the formation of granular sludge by using four different types of electron acceptors. The phosphorous uptake, denitrification, and sulfate reduction in anoxic modes were simultaneously occured because of the presence of the polyphosphate accumultating organism(PAO) that utilize nitrate and sulfate as an electron acceptor in the anoxic zone. Denitrirying phosphorous removal bacteria(DPB) was enriched under anaerobic/anoxic/aerobic condition with a nitrate as an electron acceptor, and desulfating phosphorous removal bacteria(DSPB) was enriched under anaerobic/anoxic/aerobic condition with a sulfate as an electron acceptor. Polyphosphate accumulating organism(PAO) were enriched in the anaerobic/aerobic SBR. PAO took up acetate faster than DPB and DSPB during the aerobic phase. The sludge with nitrate and sulfate as an electron acceptors grew as a granules which possessed high activity and good settleability. In the anaerobic/aerobic modes, typical floccular growth was observed. In the result of bench-scale experiment, simultaneous reactions of phosphorus uptake, denitrification and sulfate reduction were observed under anoxic condition with nitrate and sulfate as an electron acceptors. These results demonstrated that the anaerobic/anoxic modes with nitrate and sulfate as an electron acceptors played an important role in the formation of the sludge granulation.

• Journal of Korean Society of Water and Wastewater
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• v.10 no.3
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• pp.73-82
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• 1996

The removal efficiencies of organic substrates, nitrogen and phosphorus in the anaerobic-aerobic biological phosphorus removal process were investigated by addition of acetic acid, propionic acid and butyric acid which are normal volatile fatty acids contained in anaerobic digester supernatants. Substrate utilization coefficients for the phosphorus release and uptake were also estimated. The effect of a VFA, which showed higher phosphorus removal efficiency than the other VFAs did, was also studied in an anaerobic-aerobic-anoxic biological nutrient removal process. For the anaerobic-aerobic process added by VFA, the phosphorus removal efficiencies were up to about 68%, 55% and 61% for the reactors of acetic acid, propionic acid and butyric acid added, respectively, which indicates the efficiencies were increased by about 8-21%, comparing to that of 47% for the reactor with no VFA added. There were no significant difference in removal efficiencies for organic substrate and $NH_3-N$ without regard to addition of VFA. However, the removal efficiency of total nitrogen was increased in the case of VFA added, since $NO_3-N$ was less produced. For the anaerobic-aerobic-anoxic process added VFA, the removal efficiencies for $NH_3-N$ and $PO{_4}^{3-}-P$ were increased by 5% and 13%, respectively, comparing with them in the reactors not added VFA.

• Journal of Korean Society on Water Environment
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• v.22 no.3
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• pp.451-455
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• 2006

Heterotrophic biomass yield coefficients, $Y_H$, for aerobic, anoxic and anaerobic reactors were successfully estimated for the two wastewater treatment plants, where one plant was operating in the $A^2/O$ process and the other was operating in the 4-stage BNR process. The estimation of $Y_H$ was undertaken by plotting the biomass COD concentrations versus the soluble COD concentrations in order to calculate the ${\Delta}biomass$ COD/ ${\Delta}soluble$ COD in each batch reactor. The batch reactors employed in this study were fed by filtered influent and mixed liquors in the ratio of 10:1, and operated in the aerobic, anoxic and anaerobic conditions, which represented the actual operating conditions for the $A^2/O$ and 4-stage BNR process. The average $Y_H$ values of the aerobic, anoxic and anaerobic reactor for the $A^2/O$ process were 0.52, 0.41 and 0.18 mg COD/mg COD, respectively, and those for the 4-stage BNR process were 0.58, 0.40 and 0.20 mg COD/mg COD, respectively. The average ratio of the $Y_H$ for aerobic reactors to those for the anoxic reactors were about 1:0.79 for the $A^2/O$ process, and about 1:0.69 for the 4-stage BNR process. The experimental method for anoxic and anaerobic $Y_H$ estimation shown in this study has turned out to be simple and efficient in its practical application.

• Journal of Environmental Science International
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• v.6 no.6
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• pp.697-709
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• 1997

Sequencing Batch Reactor(SBR) experiments for organics and nutrients removal have been conducted to find an optimum anaerobic/anoxic/aerobic cycling time and evaluate the applicability of oxidation-reduction potential(ORP) as a process control parameter. In this study, a 61 bench-scale plant was used and fed with night-soil wastewater in K city which contained TCODcr : 10, 680 mg/l, TBm : 6, 893 mg/l, $NH_4^+-N$ : 1, 609 mg/l, $PO_4^{3-}-P$ : 602 mg/l on average. The cycling time In SBRs was adjusted at 12 hours and 24 hours, and then certainly included anaerobic, aerobic and inoxic conditions. Also, for each cycling time, we performed 3 series of experiment simultaneously which was set up 10 days, 20 days and 30 days as SRT From the experimental results, the optimum cycling time for biological nutrient removal with nlght-soil wastewater was respctively 3hrs, 5hrs, 3hrs(anaerobic-aerobic-anoxic), Nitrogen removal efficiency was 77.9%, 77.9%, 81.7% for each SRT, respectively. When external carbon source was fed in the anoxic phase, ORP-bending point indicating nitrate break point appeared clearly and nitrogen removal efficiency increased as 96.5%, 97.1%, 98.9%. Phosphate removal efficiency was 59.8%, 64.571, 68.6% for each SRT. Also, we finded the applicability of ORP as a process control parameter in SBRs.

• Journal of Animal Environmental Science
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• v.14 no.2
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• pp.113-118
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• 2008

Sequencing batch operation consists of fill, react, settle and decant phases in the same reactor. Operation consists of anaerobic, anoxic and oxic (aerobic) phases when nutrient removal from the wastewater is desired. Since the same reactor is used for biological oxidation (or mixing) and sedimentation in aerobic and anaerobic SBR operations, capital and operating costs are lower than conventional activated sludge process and conventional anaerobic digestion process, respectively. Therefore, Aerobic SBR and Anaerobic SBR operations may be more advantageous far treatment of small volume animal wastewater in rural areas.

• Journal of Environmental Science International
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• v.8 no.1
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• pp.107-113
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• 1999

A new biological nutrient removal system combining $A^2/O$ process with fixed film was developed in this work and the characteristics of denitrification were especially investigated in the combined fixed film reactor(CFFR). Media was added in the anaerobic, anoxic and aerobic reactors, respectively. Tests were made to establish the effluent level of $NO_x-N$, COD, DO and nitrite effects on $NO_x-N$ removal in the CFFR by decreasing hydraulic retention time (HRT) from 10.0 to 3.5 hours and by increasing internal recycle ratio form 0% to 200%. The influent was synthesized to levels similar to the average influent of municipal wastewater treatment plants in Korea. SARAN media with a porosity of 96.3% was packed 40% / 130% / 25% based on its reactor volume, respectively. It was found that COD rarely limited dentrification in the anoxic reactor because of high $C/NO_x/-N$ ratio in the anoxic reactor, while DO concentration in the anoxic reactor and $NO_2-N/NO_x/-N$ from the aerobic effluent inhibited denitrification in the anoxic reactor. It was proved that the critical points of DO concentration in the anoxic reactor and $NO_2-N/NO_x/-N$ from the aerobic effluent were 0.15mg/L and 10%, respectively. As the internal recycle ratio increased, DO concentration in the anoxic reactor and $NO_2-N/NO_x/-N$ from the aerobic effluent increased. Especially, at the condition of internal recycle ratio, 200%, DO concentration in the anoxic reactor and $NO_2-N/NO_x/-N$ from the aerobic effluent exceeded the critical points of 0.15mg/L and 10%, respectively. Then, denitrification efficiency considerably decreased. Consequently, it was represented that the control of DO concentration in the anoxic reactor and $NO_2-N/NO_x/-N$ from the aerobic effluent can assure effective denitrification.

• Journal of Korean Society on Water Environment
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• v.23 no.5
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• pp.689-696
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• 2007

Kinetic analysis was important to develope the biological nutrient removal process effectively. In this research, anoxic-anaerobic-aerobic system was operated to investigate kinetic behavior on the nutrient removal reaction. Nitrification and denitrification were important microbiological reactions of nitrogen. The kinetics of organic removal and nitrification reaction have been investigated based on a Monod-type expression involving two growth limiting substrates : TKN for nitrification and COD for organic removal reaction. The kinetic constans and yield coefficients were evaluated for both these reactions. Experiments were conducted to determine the biological kinetic coefficients and the removal efficiencies of COD and TKN at five different MLSS concentrations of 5000, 4200, 3300, 2600, and 1900 mg/L for synthetic wastewater. Mathematical equations were presented to permit complete evaluation of the this system. Kinetic behaviors for the organic removal and nitrification reaction were examined by the determined kinetic coefficient and the assumed operation condition and the predicted model formulae using kinetic approach. The conclusions derived from this experimental research were as follows : 1. Biological kinetic coefficients were Y=0.563, $k_d=0.054(day^{-1})$, $K_S=49.16(mg/L)$, $k=2.045(day^{-1})$ for the removal of COD and $Y_N=0.024$, $k_{dN}=0.0063(day^{-1})$, $K_{SN}=3.21(mg/L)$, $k_N=31.4(day^{-1})$ for the removal of TKN respectively. 2. The predicted kinetic model formulae could determine the predicted concentration of the activated sludge and nitrifier, investigate the distribution rate of input carbon and nitrogen in relation to the solid retention time (SRT).

• Journal of Korean Society of Water and Wastewater
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• v.16 no.6
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• pp.670-678
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• 2002

Since Korean government imposed a stricter regulation on effluent T-N and T-P concentrations from wastewater treatment plant, a new process has to be developed to meet these rules and this process should remove T-N and T-P, economically, from weak wastewater that is typical for Korea's combined sewer system sewage. In this study, a computer simulator, BioWin from EnviroSim, Inc. was used. Three processes - A2/O, Modified Johannesburg, UCT- had been simulated under same operational conditions and a new process - Parallel BNR Process - had been developed based on these simulation results. The Parallel BNR process consists of two rows of reactors: One row has anaerobic and aerobic reactors in series, and the other row has RAS anoxic1 and RAS anoxic2 reactors in series. In order to ensure anaerobic state in anaerobic tank, a part of influent is fed to RAS anoxic1 tank in second row. This process had been simulated under same conditions of other three processes and the simulation results were compared. The results showed that three existing processes could not perform biological phosphorus removal when the average influent was fed at any operation temperatures. However, the Parallel BNR process was found that biological phosphorus removal could be performed when both design and average influent were fed at any operation temperatures. This process showed the T-N concentration in effluent had a maximum value of 15mg/L when design influent was fed at $13^{\circ}C$ and a minimum value of 14mg/L when average influent was fed at $20^{\circ}C$. Also, T-P concentrations had a maximum value of 1.3mg/L when average influent was fed at $20^{\circ}C$ and a minimum value of 1.1mg/L when design influent was fed at $13^{\circ}C$. Based on these results, we found that this process can remove nitrogen and phosphorus biologically under any operational conditions.

• Journal of Microbiology and Biotechnology
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• v.18 no.12
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• pp.1958-1965
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• 2008

A denitrifying polyphosphate-accumulating bacterium (YKP-9) was isolated from activated sludge of a 5-stage biological nutrient removal process with step feed system. This organism was a Gram-negative, coccus-shaped, facultative aerobic chemoorganotroph. It had a respiratory type of metabolism with oxygen, nitrate, and nitrite as terminal electron acceptors. The 16S rRNA gene sequence of strain YKP-9 was most similar to the 16S rRNA gene sequence of Paracoccus sp. OL18 (AY312056) (similarity level, 97%). Denitrifying polyphosphate accumulation by strain YKP-9 was examined under anaerobic-anoxic and anaerobic-oxic batch conditions. It was able to use external carbon sources for polyhydroxyalkanoates(PHA) synthesis and to release phosphate under anaerobic condition. It accumulated polyphosphate and grew a little on energy provided by external carbon sources under anoxic condition, but did neither accumulate polyphosphate nor grow in the absence of external carbon sources under anoxic condition. Cells with intracellular PHA cannot accumulate polyphosphate in the absence of external carbon sources under anoxic condition. Under oxic condition, it grew but could not accumulate polyphosphate with external carbon sources. Based on the results from this study, strain YKP-9 is a new-type denitrifying polyphosphate-accumulating bacterium that accumulates polyphosphate only under anoxic condition, with nitrate and nitrite as the electron acceptors in the presence of external carbon sources.

• Journal of Environmental Health Sciences
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• v.34 no.6
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• pp.446-451
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• 2008

In this study, the removal characteristics of organic components and nutrients of sewage taken from the Suwon area were investigated in a lab-scale modified DNR (Daewoo Nutrient Removal) process. The modified DNR process consisted of a sludge denitrification tank, an anaerobic tank, an anoxic tank, an aerobic tank, a secondary anoxic tank and a secondary aerobic tank. The proposed process with the average C/N ratio of 3.5 was performed for the sewage treatment. The results were compared with other existing DNR processes. The organic fractions in sewage were analyzed by measuring the oxygen uptake rate. The resulting removal efficiencies of SS, BOD, COD, TN and TP were 93.1%, 95.5%, 86.1%, 67.8% and 80.6%, respectively.