• 제목/요약/키워드: Anopheles sinensis

검색결과 48건 처리시간 0.022초

PCR을 이용한 우리나라에서 발견되는 얼룩날개모기속 모기의 종 동정 (Species identification of the Anopheles kyrcanus complex found in Korea using PCR)

  • 용태순;이한일;이인용;이종원;황의욱
    • 한국건강관리협회지
    • /
    • 제4권1호
    • /
    • pp.68-74
    • /
    • 2006
  • For identification of four sibling species of the Anopheles hyrcanus complex found in Korea, the 5.8 rDNA-ITS2-28S rDNA region of each species was sequenced and the species-specific primers wee designed The amplified PCR products obtained from each species were analyzed by agarose gel electrophoresis. The result showed a single species- specific band, I.e. 559bp, 432bp, 322bp and 192bp for An. sinensis, An. sp., An. lesteri and An. pullus, respectively. In conclusion, the species-specific PCR primers designed from ITS2 variable regions functioned successfully and specifically, and can be applied as a useful tool for identifying species of the Anopheles hyrcanus complex found in Korea.

  • PDF

Seasonal prevalence and species composition of mosquitoes and chigger mites collected from Daegu, Gunwi and Sangju in South Korea, 2014

  • Choi, Moon Bo;Lee, Wook-Gyo;Kang, Hyun Jun;Yang, Sung-Chan;Song, Bong Gu;Shin, E-Hyun;Kwon, Ohseok
    • Journal of Ecology and Environment
    • /
    • 제41권3호
    • /
    • pp.107-113
    • /
    • 2017
  • Background: As the habitat changes in Korea due to climate change, the emergence of disease-mediated vectors is increasing rapidly. Thus for the surveillance of mosquito- and chigger mite-borne disease, their seasonal prevalence and species composition were investigated at seven locations in Daegu, Gunwi and Sangju. Methods: Mosquitoes were collected twice every month from five collection sites using a black light and BG sentinel traps in Daegu and Gunwi from April through November. Chigger mites were investigated twice per month from wild rodents caught with Sherman live traps in Gunwi and Sangju from April through May and September through November. Results: A total of 2,361 female mosquitoes were collected. Cowshed (626 individuals, Trap index (TI) 44.7) and Kyungpook National University campus (846 individuals, TI 60.4) in Daegu had the highest number of mosquitoes in the black light and BG sentinel trap, respectively. The mosquitoes were collected more by BG sentinel trap than the black light trap. Nine mosquito species were trapped, and the Culex pipiens complex was the most commonly mosquito (1,397 individuals, 59.2%), followed by Anopheles sinensis (554 individuals, 23.5%). Anopheles sinensis (531 individuals, 51.9%) and Culex pipiens complex (1,142 individuals, 85.4%) were the most mosquitoes from black light and BG sentinel trap, respectively. In terms of seasonal prevalence, the highest abundance was in July, with 824 individuals collected. In chigger mites, eighty-one wild rodents of five species that are hosts of chigger mites were collected; among them, 53 and 25 individuals of Apodemus agrarius and Crocidura suaveolens, respectively were trapped. Leptotrombidium pallidum was a dominant species, with 2,467 individuals collected (67.8%). Conclusions: The mosquito was the dominant species in Culex pipiens complex and the highest in July and August. Apodemus agrarius was most abundant in wild rats and Leptotrombidium pallidum was dominant in the collected chigger mites.

Detection of vivax malaria sporozoites naturally infected in Anopheline mosquitoes from endemic areas of northern parts of Gyeonggi-do (Province) in Korea

  • Lee, Hyeong-Woo;Shin, E-Hyun;Cho, Shin-Hyeong;Lee, Hee-Il;Kim, Chung-Lim;Lee, Wook-Gyo;Moon, Sung-Ung;Lee, Jong-Soo;Lee, Won-Ja;Kim, Tong-Soo
    • Parasites, Hosts and Diseases
    • /
    • 제40권2호
    • /
    • pp.75-81
    • /
    • 2002
  • We investigated population densities of mosquitoes infected with sporozoites in three highly epidemic areas of Josan-ri and Jangpa-ri (Paju City) and Dongjung-ri (Yeoncheon County) in Korea. Anopheline mosquitoes were collected front both indoors and outdoors by human baiting collection method during the period of the first week of June to the second week of September 1999. Total 13,296 female mosquitoes were collected and 8,650 (65.1%) were Anophelines. Thirty seven percent (3,199) of the Anopheline mosquitoes were captured outdoors and 63.9% (5,531) indoors. Employing a sandwich enzyme-linked immunosorbent assay (ELISA), we analyzed a total of 7,820 Anopheline mosquitoes and found that 7 Anopheline mosquitoes were infected with sporozoites. The positive rate in Josan-ri was 0.14% (5/3,500) and 0.15% (2/1,370) in Jangpa-ri. The total positive rate in all three surveyed areas was 0.09% (7/7,820). The mosquitoes infected with the sporozoites were detected on June $28^{th}$ (n=2), July $5^{th}$ (n=1), July $19^{th}$ (n=1), August $9^{th}$ (n=1), September $6^{th}$ (n=1), and the last one on September $13^{th}$ (n=1). They were all classified as Anopheles sinensis, which showed positive reaction in ELISA test. Therefore it might be concluded that Anopheles sinensis plays an important role in re-emerging malaria transmission in Korea.