• Journal of Animal Science and Technology
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• v.44 no.5
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• pp.617-624
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• 2002

Annual legume is being used as an important source of protein for farm animals and an ingredient for nitrogen fertilizers. There are, however, difficulties in selecting appropriate cultivar and forage management technique. The objective of this study is to select the best performing cultivars and evaluate a forage value on annual legume at two locations (Suwon and Sunghwan) for 1 year (1999${\sim}$2000). All cultivars shown a seedling vigor and lodging resistance of 8 rating or higher. Crimson and rose clover showed that they are appropriate for overwintering, but ;Laser; (35.9%) and 'Nitro'(9.1%) of persian clover variety did not overwinter well from 1999 to 2000. Flower stage was observed 4th of May for 'Tibbee', 6th of May for 'Contea' crimson clover, and 29th of April for 'Bolta balansa' rose clover. DM yield of a crimson clover 'Contea' (4,218kg/ha) was the highest among the legume forage. A crimson clover 'Tibbee' and rose clover 'Bolta balansa' also had a high production yield (3,874 and 3,828kg/ha). Calculations of relative feed value (RFV) based on the acid and neutral detergent fiber (ADF and NDF) of annual legume were over 125, which means that quality was high. According to the results of this study, crimson and rose clovers are recommended due to their high winter survival rate and high DM yield. Persian clover is not recommended because of a low overwintering rate at the Central region in Korea.

• Korean Journal of Organic Agriculture
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• v.10 no.1
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• pp.49-63
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• 2002

In order to supply 85% of total organic feed in ruminants and 80% in non-ruminants for organic animal production, nitrogen fixation ability of legume should be used in domestic roughages production. 50% of Europe organic farmer use intercropping legume in as green manure. This article is dealing with amount of biologically fixed nitrogen used by legumes and methods for estimating the transfer of biologically fixed N in rotation and separating the N benefit into fixed N and non-fixed N components are reviewed. Available data indicate that transfer amount of N to non legumes was from 50∼9.6(kg/ha) in legume-cereal rotations and proportion of legume N varied with seasons, 90% in summer, 50% in autumn. The important point in cropping system for legumes have to be included for organic forage production 6 year rotation is based on pasture system of 3 year pasture + 2 year annual(com, sudangrass), again pasture. Rye, barly and Italian ryegrass+legumes(vetch, crimson and pea) can be one of option in spring, com, soybean, sudangrass and Japanese bamyard millet would be seeded after spring harvest in the field. Farmer can make good use of rice paddy field as forge production potential area after harvesting rice. Italian, burly and rye+vetch and crimsonclover may be grown in autumn or spring time at the rice field.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.3
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• pp.289-292
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• 1997

A grazing trial was carried out to determine the effects of different grass/legume pastures on forage production, legume proportion, nutritive value, and animal performance. The pasture mixtures were mainly orchardgrass (OG) + ladino clover (T1), OG + alfalfa (T2), perennial ryegrass + alfalfa (T3), and grass alone mainly OG (T4). The pastures were rotationally grazed 7 times with heifers which had an initial liveweight of about 243 kg in 1991 and 1992. The average plant height at each grazing was 32.4 cm. The annual dry matter yield ranged from 9.19 MT in T4 to 9.61 MT/ha in T3, but no significant difference among different mixture combinations was found. The proportion of ladino clover in T1 mixtures rapidly increased from 21% to 68% during the grazing season. However, the proportion of alfalfa in T2 and T3 ranged from 12% to 23%. The contents of crude protein and calcium, respectively, in the forages were highest in T1 (23.2%, 0.68%) and lowest in T4 (18.5%, 0.13%), while crude fiber content showed the opposite trends. Liveweight gain was greatest on T1 (1.073 kg) and lowest on T4 (643 kg). It was concluded that pasture mixtures containing legume (ladino clover) can increase forage nutritive value and animal performance.

• Interdisciplinary Bio Central
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• v.3 no.1
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• pp.4.1-4.7
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• 2011

Forty-seven wild accessions of Lotus japonicus Regal (Japanese trefoil) indigenous to Japan were investigated for nine morphological characters. Average temperature and annual precipitation were negatively correlated with stem color and seed weight. On the other hand, latitude was positively correlated with these traits. Consequently, accessions from sites at higher latitudes with low temperatures and precipitation tend to have dark red stems and heavy seeds. Cluster analysis based on nine morphological characters classified 47 wild accessions into six major groups. Cluster I included four accessions of tall and erect plants. These plants are phenotypically similar to commercial variety 'Empire'. Cluster II consisted of three accessions of creep plants with pale red stems. Cluster III contained 24 accessions that had average values for all morphological characters evaluated. Cluster IV included two accessions of erect plants with rounded leaflets and dark red stems. Cluster V included four accessions of small, creep plants with pale red stems. Cluster VI included seven accessions of small and erect plants, a phenotype that also applies to ?Gifu B-129?, which is used as experimental strain worldwide. These data were deposited into LegumeBase, an online database (http://www.legumebase.brc.miyazaki-u.ac.jp/) supported by the National BioResource Project (NBRP) in Japan.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.1
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• pp.13-18
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• 2008

This experiment was conducted to compare the agronomic characteristics, flowering condition and productivity of introduced annual legumes at paddy field of Seonghwan(Cheonan, Chungnam, Korea) from September 2006 to May 2007. Annual legumes used in this study were hairy vetch(Vicia villsa, Rosa), crimson clover(Trifolium incarnatum, Contea), berseem clover(Trifolium alexandrinum, Alexandria), persian clover(Trifolium resupinatum, Prolific), balansa clover(Trifolium michelianum, Paradona), sweet clover(Melilotus officinalis, Yellow) and forage pea(Pisum sativum, Austrian). Emergency rate after seeding were 90% or more in crimson clover, hairy vetch, forage pea and berseem clover. Wintering of hairy vetch and crimson clover were excellent as 98% and 95%, respectively. Flowering rate of harvesting date(May 10) was 100% in crimson clover, 98% in balansa clover, 5% in persian clover and others were not flowering. Fresh and dry matter yield of crimson clover were highest as 72,556 kg/ha and 16,062 kg/ha, respectively. Crude protein yield of hairy vetch was highest as 2,929 kg/ha but not significant with crimson clover(2,169 kg/ha). TDN yield of crimson clover was highest as 9,007 kg/ha but not significant with hairy vetch(7,366 kg/ha). According to the results from this study, it is suggested that crimson clover would be recommendable for autumn-sowing annual legume at paddy field of Central Provinces.

• Korean Journal of Environmental Agriculture
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• v.19 no.5
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• pp.375-381
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• 2000

Quantifying the changes of soil microbial biomass and activity of enzymes are important to understand the dynamics of active soil C and N pools. The dynamics of soil microbial biomass C and N and the activity of enzymes over entire growth period of soybean-(Glycine max (L) Merr.)-wheat (Triticum aestivum L.) sequence on a Typic Haplustert as influenced by organic manure and inorganic fertilizer N were investigated in a field experiment. The application of farmyard manure at 4 to 16 $Mg{\cdot}ha^{-1}\;y^{-1}r^{-1}$ along with fertilizer nitrogen at 50 or 180 $kg{\cdot}ha^{-1}$ increased the mean soil microbial biomass from 1.12 to 2.05 fold over unmanured soils under soybean-wheat system. Irrespective of organic and chemical fertilizer N application, the soil microbial biomass was maximum during the first two months at active growing stage of the crops and subsequently declined with crop maturity. The mean annual microbial activity was significantly increased when manure and chemical fertilizer at 8 $Mg{\cdot}ha^{-1}$ and 50/180 N $kg{\cdot}ha^{-1}$, respectively were applied. The C turnover rate decreased by 47 to 72 % when the level of farmyard manure was increased from 4 to 8 and 16 $Mg{\cdot}ha^{-1}$. There were significant correlations between biomass C, available N, dehydrogenase, phosphatase and yield of the crops.

• Korean Journal of Organic Agriculture
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• v.22 no.3
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• pp.457-468
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• 2014

This study was conducted to investigate the effect of mixed-sowing of legume forage and applying of cattle manure on the productivity of rye in order to produce the organic roughage to be effectively reducing the environmental contamination and to provide Hanwoo rearing farmer with safe organic animal products. The present study also aimed to evaluate optimal applying level of cattle manure and carrying capacity of Korean native cattle (Hanwoo) per unit area. The annual amount of crude protein (CP) of rye in Gyeongju were higher than those of in Gyeongsan and Yeongju, and the mixed-sowing of forage pea was the highest (p<0.05) compared with the single-sowing. The amount of CP were increased proportionately corresponded to the applying level of fermented cattle manure, and found significantly (p<0.05) high at the level of 100 and 150 kg/ha groups. With feed value of forage, CP was tended to be higher in the mixed-sowing of legume than the single-sowing of rye at the wintering experimental sites of legume, and in particular the forage peas was higher (p<0.05) than other crops. Carrying capacity of organic Hanwoo (head/ha) was higher (p<0.05) in Gyeongju (3.28 head) than that in Gyeongsan (1.94) and Yeongju (1.50). The carrying capacity in the single-sowing of rye also was lower than that in the mixed-sowing of legume, and the forage peas was the highest among the mixed-sowing groups (p<0.05). Overall, the present results showed that it would be recommended to take into account the wintering for mix-sowing of legume. The fermented cattle manure may be applied for legume in the wintering unavailable regions. In addition, the applying the fermented cattle manure at the level of 100~150kg/ha and the mix-sowing of legume with forage peas may increase the productivity per unit area and feed value including CP for improving carrying capacity of organic Hanwoo.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.1
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• pp.52-59
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• 2014

This study was conducted to estimate the Hanwoo carrying capacity when whole crop wheat, as a winter forage crop, was grown on mixed-sowing of legume forage and by applying cattle manure on the productivity and feed value of whole crop wheat during the period of 2012~2013. The experiment was conducted in a split plot design with three replications. The main plots consisted of three different culture methods such as whole crop wheat and mixed sowing combination with hairy vetch or forage pea. The subplots consisted of four different applications of cattle manure (0, 50, 100 and 150 kg N/ha). The annual amount of dry matter (DM) of whole crop wheat in Gyeongju were higher than those of in Gyeongsan and Yeongju, and the mixed-sowing of hairy vetch mixture was the highest (p<0.05) compared with the single-sowing of whole crop wheat and mixed-sowing of legume in Gyeongju. The DM amounts were increased proportionately corresponded to the applying level of cattle manure, and was found to be significantly (p<0.05) high at the level of 100 and 150 kg/ha groups. With the feed value of forage, the crude protein (CP) contents tended to be higher in the mixed-sowing of legume than the single-sowing of whole crop wheat at the wintering experimental sites of legume. For whole crop wheat, total digestible nutrients (TDN) content was the highest in the mixed sowing plots of forage pea in Gyeongsan; however, there was no significant differences among the single-sowing of whole crop wheat. The carrying capacity of Hanwoo (head/ha) was higher (p<0.05) in Gyeongju (3.83 head) than that in Gyeongsan (3.11) and Yeongju (1.35). Further, the carrying capacity in the single-sowing of whole crop wheat was lower than that in the mixed-sowing of legume, and the hairy vetch was the highest among the mixed-sowing groups (p<0.05). Overall, the present results recommend taking into account the wintering for the mix-sowing of legume in Gyeongbuk province. The cattle manure may be applied for legume in the wintering unavailable regions. In addition, applying cattle manure at the level of 100~150 kg/ha and the mix-sowing of legume may increase the productivity per unit area and feed value, including the CP, for improving the carrying capacity of Hanwoo.

• Korean Journal of Organic Agriculture
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• v.23 no.3
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• pp.583-593
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• 2015

This study was conducted to investigate the effects of mixed-sowing of legume forage and applying of cattle manure on the productivity of organic rye to provide dairy farmers with safe organic feeds. Also the present study aimed to evaluate optimal applying level of cattle manure and carrying capacity of Korean native cattle (Hanwoo) per unit area. The amount of crude protein was significantly higher in the mixed-sowing of legume forage (average 760~786 kg/ha) than in the single-sowing of rye (average 353 kg/ha) (p<0.05). The mixed-sowing of forage peas and the single-sowing of rye tended to improve by increasing the level of nitrous fertilization rather than the fertilization treatment. The amount of crude protein on the rye according to the mixed-sowing of legume was significantly higher in the mixture (average 8.29~9.90%) than in the single (average 4.93%) (p<0.05). The comparison by the level of nitrogen fertilization indicated significantly high for the rye in 50 kg N/ha than in fertilization treatments (p<0.05). Total digestible nutrients (TDN) amount in the single-sowing of rye was average 46.86% and has lower than average 49.96~50.12% of the mixed-sowing of legume, and especially the mixed-sowing of forage pea was the highest with 54.55% in 150 kg N/ha for the level of nitrogen fertilization (p<0.05). The breeding ability of annual organic livestocks per unit area according to the feed value of rye presented significantly higher ability in the mixed-sowing of legume (3.72~4.12 heads) than the single-sowing of rye (average 2.26 heads) (p<0.05). By summarizing above results, the mixed-sowing of legume is required to improve the productivity and the feed value of rye for increasing organic livestock breeding ability in southern area of Gyeongbuk regions, and the study for identifying the appropriate fertilization level using livestock excretions is further necessary.

• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.451-458
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• 2004

No comprebensive forage quality of annual legumes harvested and cured in spring has been conducted in Korea. Therefore, this experiment was carried out to gain information on the quality of crimson clover (Trifolium incarnatum L.), bolta baIansa clover(Trifolium ba/anansae L.), and persian c1over(Trifolium resupinatum L.) during field curing in spring. The dry matter content of crimson clover at harvest was 24.7%, while bolta balansa and persian clovers had 20.4 and 18.8%, respectively. The moisture content of persian clover was low at the final curing day. But All species took 4 days to reach moisture content under 20%Tedding frequency did not affect moisture content, but consisten trends were also observed during the field curing. Persian clover tended to show a higher leaf-stem ratio than crimson and bolta balansa clovers on dry matter basis. Crude protein of persian clover(19.5%) was higher than other legumes. The percentage of erode protein was decreased from 17.8 to 16.5% as tedding frequency often did. Neutral detergent fiber(NDF) and acid detergent fiber(ADF) contents of persian clover were lower than those of other legumes. From the comparison among tedding frequency, NDF and ADF contents of three times were higher than those of one and two times. Relative feed value(RFV) of persian clover hay was the highest(178) and classified as Grade Prime in forage quality standard. Crimson and bolta balansa clovers in the RFV were also high quality as Grade 1 in forage quality standard. The RFV of legume hay was decreased from 150 to 140 as tedding frequency often did Results of the experiment indicate that hay quality of persian clover was higher than other clovers. And this is due to high leaf and stem content, hollow stem and late maturity stage. Then tedding frequency in annual legume can be teded by two times for quality.