• 제목/요약/키워드: Anisakis spp

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Larval Anisakid Infections in Marine Fish from Three Sea Areas of the Republic of Korea

  • Cho, Shin-Hyeong;Lee, Sang-Eun;Park, Ok-Hee;Na, Byoung-Kuk;Sohn, Woon-Mok
    • Parasites, Hosts and Diseases
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    • 제50권4호
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    • pp.295-299
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    • 2012
  • The present study was performed to determine the infection status of anisakid larvae in marine fish collected from 3 sea areas of the Republic of Korea. Total 86 marine fish (8 species) collected from the East Sea (Goseong-gun, Gangwon-do), 171 fish (10 species) from the South Sea (Sacheon-si, Gyeongsangnam-do), and 92 fish (7 species) from the Yellow Sea (Incheon Metropolitan City) were examined by both naked eyes and artificial digestion method. Among the total of 349 fish examined, 213 (61.0%) were infected with 8 species of anisakid larvae, i.e., Anisakis simplex, 6 types of Contracaecum spp., and Raphidascaris sp., and the mean larval density was 13.8 per infected fish. Anisakid larvae were detected in 45 fish (52.3%) from the East Sea, 131 fish (76.6%) from the South Sea, and 37 fish (40.2%) from the Yellow Sea. The average numbers of larvae detected were 4.0, 16.6, and 15.9, respectively. Anisakis simplex larvae were detected in 149 fish (42.7%), and the mean larval density was 9.0 per infected fish. They were found in 26 fish (30.2%) collected from the East Sea, 96 fish (56.1%) from the South Sea, and 27 fish (29.3%) from the Yellow Sea. The average numbers of larvae detected were 2.9, 10.3, and 10.5, respectively. Conclusively, the present study suggests that the infection rate and density of anisakid larvae are more or less higher in the fish from the South Sea than those from the East Sea or the Yellow Sea.

Infection status with helminthes in feral cats pur-chased from a market in Busan, Republic of Korea

  • SOHN Woon-Mok;CHAI Jong-Yil
    • Parasites, Hosts and Diseases
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    • 제43권3호
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    • pp.93-100
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    • 2005
  • The present study was performed to investigate the infection status with helminth in a group of feral cats in Korea. More than 29 helminth species including adults or eggs were detected in visceral and fecal samples of the examined cats. Among these were a host of nematodes, including toxocarids, Ancylostoma sp. and the larva of Anisakis simplex; trematodes, including Clonorchis sinensis, Paragonimus westermani, Eurytrema pancreaticum, Pharyngostomum cordatum, Metagonimus spp., Heterophyes nocens, Pygidiopsis summa, Heterophyopsis continua, Stictodora fuscata, Stictodora lari, Acanthotrema felis, Stellantchasmus falcatus, Centrocestus armatus, Procerovum varium, Cryptocotyle sp., Echinostoma revolutum, Echinostoma hortense, Echinochasmus japonicus, Stephanoprora sp., Plagiorchis muris, Neodiplostomum sp. and diplostomulum. We also detected a variety of cestodes, including Spirometra erinacei, Taenia taeniaeformis and unidentified species of tapeworm. We also found examples of the acanthocephalan, Bolbosoma sp. In our assessment of the stools, we detected at least 12 species of helminth eggs. These findings confirmed that feral cats in Korea are infected with a variety of helminth parasite species. Furthermore, among the helminths detected, E. pancreaticum, S. fuscata, S. lari, A. felis, S. falcatus, C. armatus, P. varium, Cryptocotyle sp., E. revolutum, E. japonicus, Stephanoprora sp., P. muris, Neodiplostomum sp. and Bolbosoma sp. represent helminth fauna which have not been reported previously in feral cats in the Republic of Korea.

고래회충유충증 감별 진단을 위한 18S ribosomal DNA (rDNA) PCR-RFLP 법 적용 (Application of the 18S Ribosomal DNA (rDNA) PCR-RFLP Technique for the Differential Diagnosis of Anisakidosis)

  • 김선미;조민경;유학선;차희재;옥미선
    • 생명과학회지
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    • 제19권9호
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    • pp.1328-1332
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    • 2009
  • 고래회충유충증은 해산어류에 기생하는 고래회충과(family Anisakidae)에 속하는 선충류 유충에 의한 질병으로 유충의 직접적인 위장관내 침입으로 인한 병변과 더불어 유충의 분비 배설물에 의한 알레르기 질환도 유발될 수 있다. 고래회충유충증은 A. simplex를 비롯하여 Contracaecum, Pseudoterranova, Hysterothylacium 등의 유충에 의해 야기될 수 있으나 이들에 대한 형태학적 감별 진단은 유충의 형태적 유사성으로 인하여 매우 어려운 경우가 많다. 이러한 형태학적 진단의 어려움을 극복하고 분자생물학적 감별진단 방법을 확립하기 위하여 A. simplex, Contracaecum type A. type C' 및 Goezia 유충을 숭어, 도다리, 고등어, 아나고, 참돔 등 5종의 어류에서 분리하였다. 각각의 유충으로부터 분리한 18S rDNA를 PCR로 증폭한 후 Taq 1, Hinf I, Hha I, Alu 1, Dde I, Hae III, Sau 96I, Sau 3AI 등 8종의 제한효소를 사용하여 PCR-RFLP를 시행하였다. PCR product의 크기는 약 2.0 Kb였으며 Hinf l, Alu 1, Hha I, Dde 1 및 Hae III로 A. simplex와 Contracaecum type C'을 구분할 수 있었다. 그러나 Contracaecum type A의 경우에는 Taq I, Hinf I, Alu I 및 Dde I의 경우에는 2가지 패턴으로 나타났으며 이들 가운데 일부는 A. simplex, Contracaecum type C', 및 Goezia와 동일한 분석 패턴을 보이기도 하였다. Goezia는 사용한 8개의 제한 효소 모두에서 A. simplex 및 Contracaecum type A 및 type C'과 각기 다른 양상을 보였다. 이러한 결과로 18S rDNA PCR-RFLP 방법은 A. simplex와 Contracaecum type C'의 감별 진단에 유용한 것으로 밝혀졌으며, Contracaecum type A의 분류에는 제한적으로 사용되어야 함은 물론 형태학적 분류 기준에 대한 재검토가 뒤따라야 할 것으로 사료되었다.

중국 연변지역 주요 기생충질환에 대한 혈청역학적 조사 (Seroepidemiology of helminthic diseases in Yanbian of the People's Republic of China)

  • 주경환;전복실;권혜수;조성원;이순옥
    • 농촌의학ㆍ지역보건
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    • 제23권2호
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    • pp.275-285
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    • 1998
  • 간흡충(Clonorchis sinensis), 폐흡충(Paragonimus westermani), 스팔가눔(sparganum), 뇌낭미충(Cysticercus cellulosae), 아니사키스(Anisakis spp.), 선모충(Trichinella spiralis), 개화충(Toxocara canis)에 대한 IgG항체반응을 ELISA를 이용하여 연변지역주민의 혈청역학적 조사를 시행하고 그 결과를 PC-SAS 시스템으로 통계분석하여 다음과 같은 결과를 얻었다. 1995년 10월부터 1997년 6월 사이에 수집된 총 975명의 혈청에 대한 검사결과 23.87%에서 한 종류이상의 혈청항체 양상을 나타내였다. 이중 간흡충증은 5.74%, 폐흡충증은 4.92%, 낭미충증은 1.54%, 스팔가눔증은 8.51%, 아니사키스증은 1.85%, 개회충증은 12.51%, 선모충증은 7.08%의 혈청항체 양성률을 나타내었다. 판정된 양성혈청을 통계분석한 결과 간흡충(p<0.01), 혜흡충(p<0.01), 아니사키스(p<0.05), 개회충(p<0.01), 선모충(p<0.01)의 각 연령그룹비교에서 통계학적으로 유의한 차이를 나타내었고 스팔가눔증은 성별 사이에 유의한 차이가 보였다(p<0.05). 간흡충과 폐흡충은 50대 연령층에서 높은 양성항체가를 나타내는 반면 개회충과 선모충은 20대에서 높은 항체가를 보여주었다. 또한 내과질병으로 않고 있는 환자혈청은 낭미충증을 제외한 6종류 윤충류에서 정상대조인과 비교하여 볼 때 모두 통계학적으로 유의한 차이를 나타내었다(p<0.05 혹은 p<0.01). 즉 환자가 정상대조인보다 혈청양성률이 훨씬 높았다. 이상의 결과는 물론 선충사이의 교차반응이 있을거라고 추측되며 정확한 진단을 위해서는 EITB를 시행해야 한다고 생각한다. 이번 조사는 중국 연변에서 처음으로 되는 혈청역학조사이며 앞으로 기생충진환 퇴치대책에 아주 중요한 기초자료가 될 수 있을 것으로 생각한다.

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한국(韓國)에 있어서의 장내기생충감염(腸內寄生虫感染)의 현황(現況)과 추이(推移) (Current Status and Transition of the Prevalence of Intestinal Parasitic Infections in Korea)

  • 김동찬
    • 농촌의학ㆍ지역보건
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    • 제9권1호
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    • pp.83-108
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    • 1984
  • Out of a total of 58 species of helminthic and protozoan parasitic infections in Korea, so far 38 species were reported as intestinal parasites of man. Quite a few species of the intestinal parasitic infections have long been prevalent throughout the country and this has been a significant public health problem. In this paper, current status and transition of the intestinal parasitic infections in the past years were presented. Chronological reviewing of data show background and prospects of change in the prevalence of infections. In the national prevalence survey on parasitic infections conducted once every five years since 1971, stool examinations were done by both cellophane thick smear and brine flotation techniques. Every egg positive case of Ascaris lumbricoides and Clonorchis sinensis was further examined by Stoll's dilution egg counting technique. In 1981, perianal swab using adhesive cellulose tape was added for Enterobius infection. For protozoan cyst examination conducted by province and city in '81, fecal specimens were fixed in SAF solution and examined by the formalin-ether concentration technique. High prevalence of parasitic infection in ana before the 1960s can be easily understood from the data given by the Ministry of Health and Social Affairs in 1967. From a parasite control point of view, the 1960s was the preparatory period particularly for control of soil-transmitted helminthiasis. Several organizations which have contributed to parasite control were founded in this period and the prevention law of parasitic infections was passed in '66. In the '70s, overall prevalence rates of the common intestinal parasitic infections, which were highly prevalent in the past were turned into reduction phase for the first time. The '80s can be said to be an active control period of parasitic infections. Intestinal helminths According to the reports of the national prevalence survey on parasitic infections, the prevalence of helminthic infections was 84.3%(number of person examined:24,887) in '71, 63.2%(27,178) in '76, and 41.1%(35,018) in '81. By area, the prevalence rate in '81 was 35.1%(20,569) in urban areas and 49.6%(14,447) in rural areas. Intestinal nematodes Ascaris lumbricoides The prevalence of Ascaris infection has decreased significantly in recent years. Among students, the prevalence was 55.4%, in '69 and decreased to 4.7% in '83. In the national prevalence surveys, the prevalence decreased to 13.0% in '81 from 54.9% in '71. By area, the prevalence decreased to 8.5% in '81 from 46.4% in '71 in urban area and 19.4% in '81 from 59.6% in '71 in rural area. By age, the prevalence has become in recent years relatively even in all age groups, although higher prevalence used to be seen in young age groups of around 10 years old, particulary in the highly prevalent rural areas. By sex, the prevalence was higher in the female than in the male. Unfertilized egg positive rates among the ascariasis cases increased gradually up to 55.4% on the average in '81. The intensity of the infection was also significantly decreased. Trichuris trichiura Trichuris infection had also decreased to 23.4% in '81 from 65.4% in '71. By area, the decreasing tendency of the prevalence became faster in urban areas than in the rural areas. The prevalence in urban and rural areas in '71 was 69.7%, and 63.1% respectively and decreased to 19.5% and 29.0% respectively in '81. By age, the prevalence reached a peak at the 10-14 age group and showed relatively even distribution throughout all age groups. By sex, the prevalence was close in young age groups, but in the 30s or over age group, especially in rural area, the prevalence was significantly higher in the female than in the male. The prevalence has much fluctuated depending in the area. The prevalence in rural areas surveyed in the '80s shows a range between 20.9% and 73.7% by locality. It is anticipated that the prevalence of Trichuris infection will drop more rapidly, when mass treatment is conducted. Hookworms Hookworm infection by mostly Ancylostoma duodenale and a few by Necator americanus has decreased to a negligible levels in recent years. In the national prevalence surveys, the prevalence was 10.7% in '71, 2.2% in '76, and 0.5% in '81. The prevalence was higher in rural areas than in urban areas. Wide application of multi-specific anthelminthics in the ascariasis control programmes conducted in the past decade appear to have been effective against hookworm infection. Trichostrongylus orientalis As in the case with hookworm infection, the prevalence of Trichostrongylus infection has reached a negligible levels. In the national prevalence surveys, the prevalence was 7.7% in '71, 1.0% in '76 and 0.2% in '81. Enterobius vermicularis In the national prevalence survey in '81, the egg positive rate was 12.0%. Higher prevalence is expected when examined repeatedly. The prevalence rate was 10.3% in urban area and 14.6% in rural area. In recent surveys conducted in rural areas among schoolchildren, the prevalence was 32.4% in Gimhae Gun in '82 and 64.1% in Yeongyang Gun in '83. By age, the egg positive rate was higher in young age groups of around 10 and sharply decreased in age groups of around 20 and then somewhat increased again in middle age groups. By sex, the prevalence was higher in the female than in the male. Strongyloides stercoralis Strongyloides stercoralis infection has rarely been found in Korea. Three cases were reported in 1914. And 0.1-0.5% were found infected out of 2,642 persons examined at the prisoner-of-war camp on Geojedo in 1956. One case was reported in '54 and '82, respectively. Anisakis spp. No systematic survey has been conducted for anisakiasis In Korea. So far, only several cases have been found 1 case in Seoul in '71, 5 cases in Busan in '81 and 1 case in Busan in '84. Intestinal trematodes Metagonimus yokogawai In the national prevalence survey conducted in 1981, the egg positive rate was 1.24% on the average. High endemic areas are located in the southwestern part of Korea. The prevalence in Hadong Gun was 29.1% on the average in '79. In a survey conducted in 76, the prevalence was 44.0% in Gwangyang, 55.0% in Gogseung and 29.0% in Gurye. The infection is closely correlated with raw sweetfish consumption in these areas. Other intestinal trematodes A human case of Heterophyes heterophyes was reported in 1914. Several species were reported in the '80s : 17 cases of Fibricola seoulensis, 9 cases of Pygidiopsis summa, 8 cases of Heterophyes heterophyes nocens, 1case of Heterophyopsis continua, 2 cases of Stellantchasmus falcatus, 1 case of Stictodora sp., 1 case of Echinostoma hortense, and 4 cases of Echinochasmus japonicus. As the intermediate hosts, snakes and frogs play a role for F.seoulensis and fish for the rest of the species. Intestinal cestodes Taenia saginata and T. solium Egg positive rates in the national prevalence survey were 0.7% in '76 and 1.1% in '81. The prevalence in '81 was 0.6% in urban area and 1.8% in rural area. The proglottid positive rate in Jeju Do was 19.2% on the average. On Udo, Jeju Do in 1983, the egg positive rate among the inhabitants was 2.9%. Hymenolepis nana In the national prevalence survey, egg positive rates were 0.6% in '76 and 0.4% in '81. No difference was seen in the prevalence by area and sex. Hymenolepis diminuta Infected cases were reported : 3 in '64 and I in '66. Egg positive rate in '81 was 0.01% in the national prevalence survey. Diphylobothrium latum So far, about 30 cases have been reported. The cases have been reported more frequently in recent surveys. Mesocestoides sp. A case was reported from a hospitalized patient in Seoul in '67. Spirometra erinacei Two cases were reported in '84 following reidentification of the adult worms collected in '74. Intestinal protozoa Out of a total of 23 species of human protozoan infections in Korea, 13 species were reported as intestinal protozoa : Entamoeba histolytica, E coli, Endolimax nana, Iodamoeba b$\ddot{u}$tschlii, Dientamoeba fragilis, Giardia lamblia, Chilomastix mesnilii, Embadomonas sp., Enteromonas hominis, Trichomonas hominis, Isospora belli, I. Hominis(Sarcocystis hominis), and Balantidium coli. Since the first report on intestinal protozoan infections in 1925, there have been quite a few survey data on the prevalence of the infection. It was found reviewing the data chronologically that up to the early '70s the infection was prevalent around a 30-50%. After that, the protozoan cyst positive rate has shown the tendency of gradual decrease throughout the country. Protozoan cyst survey conducted in Seoul and several provinces in 1981 revealed infection rates of 8.9%(1,310) in Gangweondo, 10.7%(1,703) in Gyenggi Do, 11.7%(1,032) in Jeonra Buk Do, 9.1%(4,116) in Jeonra Nam Do, and 1.4%(5,275) in Seoul. Entamoeba histolytica In the survey conducted by province in '81, the cyst positive rate was 0.8% in Gangweon-do, 0.3% in Gyeonggi Do, 1.4% in both Jeonra Buk Do and Jeonra Nam Do, and 0.2% in Seoul. Giardia lamblia In the survey by province in '81, cyst positive rates were 2.2% in both Gyeonggi Do and Jeonra Buk Do, 1.9% in Jeonra Nam Do, 0.5% in Gangweon Do, and 0.9% in Seoul. Balantidium coli Two cases were reported. One in 1930 and the other in '74. Isospora belli and I. Hominis(Sarcocystis hominis) Isospora belli was reported : 1 case in '56 and 3 cases in '66. I. Hominis, recently identified to be synonymous with Sarcocystis hominis, was reported : 3 cases in '66. Other intestinal protozoa The protozoan parasites other than the above mentioned are generally treated as commensal, although some of them are considered to be pathogenic. The data of '81 show that about 10% of the inhabitants are still infected with protozoa.

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