• Biomolecules & Therapeutics
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• v.19 no.2
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• pp.231-236
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• 2011

The aim of this study was to investigate the effect of charge carrier lipid on the skin penetration, retention, and hair growth of topically applied finasteride-containing liposomes. Finasteride-containing liposomes were prepared by traditional thin film hydration method using Phospholipon$^{(R)}$ 85 G and cholesterol with or without charge carrier lipid (1,2 dimyristoyl-sn-glycero-3-phosphate or 1,2-dioleoyl-trimethylammonium-propane for anionic and cationic charge, respectively). Freshly prepared finasteride-containing liposome suspension was applied on the hairless mouse skin, and skin penetration and retention were measured using Keshary-Chien diffusion cell. Non-liposomal formulation (ethanol 10% solution containing 0.5 mg/ml of FNS) was also used as a control. The amount of finasteride in the diffusion cell and mouse skin was measured by HPLC. The hair growth was evaluated using depilated male C57BL/6N mice. Mean particle size of all finasteride-containing liposomes was less than a micron, and polydispersity index revealed size homogeneity. Skin penetration and retention studies showed that significantly less amount of finasteride was penetrated when applied as anionic liposome while more amount of the drug was retained. Specifically, in liposome prepared with 10% anionic charge carrier lipid, penetration was 12.99 ${\mu}g/cm^2$ while retention was 79.23 ${\mu}g/cm^2$ after 24 h of application. In hair growth study, finasteride-containing anionic liposomes showed moderate efficacy, but the efficacy was not found when applied as cationic liposomes. In conclusion, topical application of finasteride using anionic liposome formulation appears to be useful option for the treatment of androgenetic alopecia to avoid systemic side effects of the drug.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.1
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• pp.83-89
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• 2008

Eighty-four Holstein cows were used to evaluate effects of feeding two diets that differed in dietary cation-anion difference (cationic; +28 or anionic; -138 mEq/kg DM) on prepartum and postpartum dry matter intake (DMI), body weight (BW), body condition score (BCS), serum Ca concentrations and on subsequent milk production and composition. Treatments were in a $2{\times}3{\times}2$ factorial arrangement that included prepartum diet, dry period length (30 d dry, 30 d dry+estradiol cypionate (ECP), and 60 d dry), and prepartum and postpartum bST ($POSILAC^{(R)}$ 10.2 mg/d). No interaction of prepartum diet with dry period length or bST supplementation was detected for any measure evaluated either prepartum or postpartum. No significant effects of prepartum diet on prepartum DMI, BW or BCS were observed. Mean DMI during the first 28 d postpartum were similar for cows fed the cationic or anionic diets prepartum (25.5 vs. 26.1 kg/d). During postpartum wk 1 to 14, no differences in mean BW or BCS were detected due to prepartum diet fed but decreases for both groups were observed during the first 6 wk postpartum. No differences due to prepartum diet were observed for mean milk or 3.5% FCM yields or for milk composition during the first 10 wk of lactation. Similarly, mean milk yield of cows during the first 21 wk did not differ significantly due to prepartum diet fed (38.5 vs. 38.6 kg/d). Overall, cows fed the prepartum cationic or anionic diets had similar mean postpartum serum concentrations of Ca (9.34 vs. 9.35 mg/dl). Subsequent milk production, milk composition and concentrations of Ca did not differ. Importantly, the two prepartum diets were equally satisfactory in minimizing incidence of milk fever and in supporting initiation of lactation, irrespective of dry period length and supplemental ECP and bST.

• Journal of Pharmaceutical Investigation
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• v.34 no.3
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• pp.177-183
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• 2004

Recently, studies on intranasal mucosa delivery of influenza vaccine have been actively developed because of lack of pain and ease of administration. We studied on preparation of nanoparticle delivery system using biodegradable polymer as a poly(DL-lactide-co-glycolide) (PLGA) and their binding characteristics with vaccine. Three kinds of PLGA nanoparticles were prepared by spontaneous emulsification solvent diffusion (SESD) method using sodium dodecyl sulfate and sodium laurate as an anionic surfactant and Lutrol F68 (polyethylene glycol-block-polypropylene glycol copolymer) as a nonionic surfactant. The 5-aminofluorescein labeled vaccine was coated on the surface of nanoparticles by ionic complex. The complexes between vaccine and nanoparticles were confirmed by change of the size. After vaccine coating on the surface of anionic nanoparticles, particle size was increased from 174 to 1,040 nm. However the size of nonionic nanoparticles was not more increased than size of anionic nanoparticles. The amount of coated vaccine on the surface of PLGA nanoparticles was $14.32\;{\mu}g/mg$ with sodium dodecyl sulfate, $12.41\;{\mu}g/mg$ with sodium laurate, and $9.47{\mu}g/mg$ with Lutrol F68, respectively. In conclusion, prepared nanoparticles in this study is possible to use as a virus-like nanoparticles and it could be accept in the field of influenza vaccine delivery system.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.34 no.4
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• pp.7-15
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• 2002

It is of critical importance to understand the characteristics of papermaking additives and their reaction mechanisms to fully utilize the benefits they provide. Among the papermaking additives, retention aids play critical roles in improving productivity, product quality and process economy. Diverse research efforts to understand the reaction mechanisms between cationic polymers and anionic microparticles have been made since microparticle retention systems were introduced into the market. And it is most commonly accepted that flocs formed by the addition of cationic polymers are dispersed by shear force and the broken flocs are reflocculated instantly with the addition of microparticles. There are still many unanswered questions, however, on the reaction phenomena between cationic polymers and anionic microparticles. In this study, several cationic polymers including waxy maize starch, com starch and guar gum were used to investigate their retention efficiency when they were used along with anionic colloidal silica.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.30 no.2
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• pp.30-39
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• 1998

Effect of cationic starches and anionic colloidal silica on retention and drainage characteristics of Compozil system was investigated. Depending upon the degree of substitution and molecular weight of cationic starches and morphological characteristics of anionic colloidal silica, retention and drainage properties of Compozil system were significantly influenced. When cationic starch addition level increased above a certain limit retention and stock freeness were decreased. To elucidate this an electrostatic coagulation mechanism occurring between unadsorbed starch molecules and anionic colloidal silica was proposed. Unstructured colloidal silica showed greater improvement in retention than structured colloidal silica. Cationic corn starches with different degree of substitution and molecular weights were prepared and their effect as a constituent of Compozil system was also evaluated. By controlling the molecular weight and degree of substitution of cationic corn starch it was possible to achieve significant improvement in fines retention. Cationic corn starch with higher degree of substitution maintained its retention efficiency even when the stock conductivity was increased. Turbidity measurement technique was found to be a simple and useful method to measure the retention characteristics.

• Journal of Environmental Science International
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• v.5 no.3
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• pp.369-376
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• 1996

The adsorption of the anionic surfactants, sodium lauryl sulfate (SLS) and sodium dodecylbenzene sulfonate (SDBS) anion surfactants form aqueous solutions with nonionic resins, Amberlite XAD-2, XAD-4 and XAD-7 at temperatures in 15~45$^{\circ}C$ range was studied. Several adsorption isotherm models were used to fit the experimental data, The best results were obtained with the Redlich-Peterson equation and the Freundlich model provided remarkably good fits. For a particular resin at a particular temperature, SDBS was more extensively adsorbed than SLS. The highest adsorption were obtained with XAD-4 resin and the specific surface area of the resins plays a major role in adsorption of the surfactants. Estimations of the isosteric heat of adsorption were indicative of an exothermic process, and their magnitudes manifested a physisorption process.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.31 no.3
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• pp.10-18
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• 1999

The influence of simple electrolyte or anionic trashes on cationic starch adsorption was examined using deinked pulp and bleached thermomechniical pulp. Adsorbed amounts of cationic starches increased slightly , then decreased abruptly as the concentration of simple electrolyte increased. This phenomenon was discussed based on the compression of electrical double layer and conformation change of starch molecules. Also, the effect of the type of simple electrolytes on starch adsorption was examined and discussed. Addition of sodium slilicate increased starch adsorption since it increased surface charge density of fibers. On the other hand, addition of kraft lignin decreased cationic demand of the pulp slurries and adsorbed amounts of cationic starches. Nonionic surfactant did not show any significant effect on the cationic demand of pulp slurries. When simple electrolytes were added to the stock , grater adsorption or cationic starches was obtained with starches of lower degree of substitution . On t도 other hand, amount of adsorbed starches decreased for lower DS starches when the concentration of anionic trashes increased.

• Bulletin of the Korean Chemical Society
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• v.32 no.9
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• pp.3411-3420
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• 2011

Kinetic and structural studies have been carried out on the effects of meso-tetrakis(4-sulfonatophenyl)-porphyrin ($H_2TPPS_4$) as an anionic and meso-tetrakis(3-N-methyl-pyridyl)porphyrin ($H_2TMPYP$) as a cationic porphyrin with adenosine deaminase (ADA) in 25 mM citrate/phosphate buffer, pH = 4-8, at $37^{\circ}C$ using UVvis spectrophotometry, circular dichroism (CD), fluorescence spectrophotometry as well as molecular dynamics (MD) and molecular docking. Kinetic results showed that the two porphyrins are non-competitive inhibitors. Increasing pH, increases $K_I$ and cationic porphyrin has a higher $K_I$ and lower binding constant ($K_b$) at all pH ranges. Analyzing the secondary structure revealed that both ligands decrease the secondary structure and that the anionic porphyrin is more effective.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 1999.11b
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• pp.125-129
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• 1999

This paper dealt with effect of dual component and micropartical retention system on papermaking. First-pass retention under dynamic drainage condition was studied in neutral and alkaline papermaking system. Cationic starches, amphoteric starches and amphoteric polyacrylamide were added prior to high shear force, then anionic. The system is found to be very useful to inprove filler retention. For mitigating unfavorable effect of interfering sub-stances, anionic trash catcher(ATC) such as p-DADMAC was tested in this study.

• Journal of the Korean Society of Clothing and Textiles
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• v.3 no.1
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• pp.13-18
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• 1979

Many researches have found that the anionic surfactants are effective when the anionic soil is attached to the cotton fabrics. However, this research investigated the relationship of the super soil removal and surfactants when the anionic and cationic soil was attached to the cationic cotton fabrics. The result is that the cationic surfactants are vary effective for soil removal in the cationic cotton fabrics. The processing and nature of cationic cotton fabrics are treated and investigated as follows: Cotton fabrics are heated in the presence of ethylenimine and acetic acid dissolved in benzene to contain a significant amount of fixed nitrogen. Some polymer was formed but removal by washing with benzene and water. The optinium molor ratio of acid-to-ethylenimine seemed to be in the range 1: 10. The treated cotton fabrics dyed with acid Orange II dyes, and nitrogen content in the treated cotton fabrics were determined by the Kjeldahl method.