• Title/Summary/Keyword: Animal-fats

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Transesterification Using the Cross-Linked Enzyme Aggregate of Photobacterium lipolyticum Lipase M37

  • Han, Jin-Yee;Kim, Hyung-Kwoun
    • Journal of Microbiology and Biotechnology
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    • v.21 no.11
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    • pp.1159-1165
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    • 2011
  • Biodiesel is methyl and ethyl esters of long-chain fatty acids produced from vegetable oils or animal fats. Lipase enzymes have occasionally been used for the production of this biofuel. Recently, biodiesel production using immobilized lipase has received increased attention. Through enhanced stability and reusability, immobilized lipase can contribute to the reduction of the costs inherent to biodiesel production. In this study, methanol-tolerant lipase M37 from Photobacterium lipolyticum was immobilized using the cross-linked enzyme aggregate (CLEA) method. Lipase M37 has a high lysine content (9.7%) in its protein sequence. Most lysine residues are located evenly over the surface of the protein, except for the lid structure region, which makes the CLEA preparation yield quite high (~93%). CLEA M37 evidences an optimal temperature of $30^{\circ}C$, and an optimal pH of 9-10. It was stable up to $50^{\circ}C$ and in a pH range of 4.0-11.0. Both soluble M37 and CLEA M37 were stable in the presence of high concentrations of methanol, ethanol, 1-propanol, and n-butanol. That is, their activities were maintained at solvent concentrations above 10% (v/v). CLEA M37 could produce biodiesel from olive oil and alcohols such as methanol and ethanol. Additionally, CLEA M37 generated biodiesel via both 2-step methanol feeding procedures. Considering its physical stability and reusability, CLEA M37 may potentially be used as a catalyst in organic synthesis, including the biodiesel production reaction.

Desalinated underground seawater of Jeju Island (Korea) improves lipid metabolism in mice fed diets containing high fat and increases antioxidant potential in t-BHP treated HepG2 cells

  • Noh, Jung-Ran;Gang, Gil-Tae;Kim, Yong-Hoon;Yang, Keum-Jin;Lee, Chul-Ho;Na, O-Su;Kim, Gi-Ju;Oh, Won-Keun;Lee, Young-Don
    • Nutrition Research and Practice
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    • v.4 no.1
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    • pp.3-10
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    • 2010
  • This study was performed to investigate the effect of desalinated underground seawater (named as 'magma seawater', MSW) of Jeju Island in Korea on lipid metabolism and antioxidant activity. MSW was collected from underground of Han-Dong in Jeju Island, and freely given to high fat diet (HFD)-fed C57BL/6 mice for 10 weeks. Although there were no significant differences in the body weight changes and plasma lipid levels, hepatic triglyceride levels were significantly lower in the MSW group than in the normal tap water (TW)-drunken control group. Furthermore, the activity of fatty acid synthase (FAS) was significantly decreased and carnitine palmitoyltransferase (CPT) activity was increased in MSW group compared to TW group. Similarly, real-time PCR analysis revealed that mRNA expressions of lipogenic genes were lowered in MSW groups compared to the control group. In a morphometric observation on the liver tissue, accumulation of fats was remarkably reduced in MSW group. Meanwhile, in vitro assay, tree radical scavenging activity measured by using diphenylpicrylhydrazyl (DPPH) was increased in MSW group. The 2'-7'-dichlorofluorescein diacetate (DCF-DA) staining followed with fluorescent microscopy showed a low intensity of fluorescence in MSW-treated HepG2 cells, compared to TW-treated HepG2 cells, which indicated that the production of reactive oxygen species by tert-butyl hydroperoxide (t-BHP) in HepG2 cells was decreased by MSW treatment. The antioxidant effect of MSW on t-BHP-induced oxidative stress in HepG2 cells was supported by the increased activities of intracellular antioxidant enzymes such as catalase and glutathione reductase. From these results, we speculate that MSW has an inhibitory effect on lipogenesis in liver and might play a protective role against cell damage by t-BHP-induced oxidative stress.

Improvement of Low-temperature Fluidity of Biodiesel from Vegetable Oils and Animal Fats Using Urea for Reduction of Total Saturated FAME (요소 이용 포화도 저감을 통한 동.식물성 바이오디젤의 저온유동성 개선)

  • Lee, Yong-Hwa;Kim, Kwang-Soo;Jang, Young-Seok;Shin, Jung-Ah;Lee, Ki-Teak;Choi, In-Hu
    • Journal of the Korean Applied Science and Technology
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    • v.31 no.1
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    • pp.113-119
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    • 2014
  • The compositions of saturated and unsaturated fatty acids in biodiesel feedstocks are important factors for biodiesel properties including low-temperature fluidity and oxidative stability. This study was conducted to improve low-temperature fluidity of biodiesel by reducing total saturated FAME (fatty acid methyl ester) in animal fat biodiesel fuels via urea-based fractionation and by mixing plant biodiesel fuels (rapeseed-FAME, waste cooking oil-FAME, soybean-FAME, and camellia-FAME) with enriched-polyunsaturated FAME derived from animal fat biodiesel. Our results showed that the reduction of total saturated FAME in animal fat biodiesel lowered CFPP (Cold Filter Plugging Point) to $-15^{\circ}C$. Mixing plant biodiesel fuels with the enriched-polyunsaturated FAME derived from animal fat biodiesel lowered CFPP of blended biodiesel fuels to $-10{\sim}-18^{\circ}C$.

Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids

  • Mulyani, Sri;Setyabudi, Francis.M.C. Sigit;Pranoto, Yudi;Santoso, Umar
    • Food Science of Animal Resources
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    • v.37 no.5
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    • pp.708-715
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    • 2017
  • The acid pretreatment of collagen molecules disrupts their crosslinks and assists in the release of acid-soluble proteins, fats, and other components. Generally, to achieve optimum extraction efficiency, strong acids may be used at a lower acid concentration compared to weak acids. This study aimed to determine the yield and physicochemical properties of gelatins extracted from buffalo hides pretreated with different acids. Hides were extracted with hydrochloric, citric, and acetic acids at concentrations of 0.3, 0.6, 0.9, 1.2, and 1.5 M. A completely randomized design and the least significant difference test were used in the experimental design, and all measurements were performed in triplicate. The highest yield (29.17%) was obtained from pretreatment with 0.9 M HCl. The gel strength did not differ significantly (p>0.05) according to acid type (280.26-259.62 g Bloom), and the highest viscosity was obtained from the 0.6 M citric acid pretreatment. All the gelatins contained ${\alpha}$- and ${\beta}$-chain components and several degraded peptides (24-66 kDa). The color and Fourier-transform infrared spectrum of the gelatin extracted using 0.9 M HCl were similar to those of commercial bovine skin gelatin. In general, the physicochemical properties of the gelatin complied with the industry standard set by the Gelatin Manufacturers Institute of America, revealing that buffalo hide could serve as a potential alternative source of gelatin.

Evaluation of Diet Quality according to Food Consumption between Highly Educated, Married, Unemployed and Employed Women (고학력 기혼여성의 취업여부별 식품섭취상태로 본 식사의 질 평가 - 전업주부와 교사의 비교 -)

  • Choi Ji-Hyun;Chung Young-Jin
    • Journal of Nutrition and Health
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    • v.39 no.3
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    • pp.274-285
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    • 2006
  • The differences in food consumption between highly educated, married, unemployed and employed women were analyzed by food group and common food item intake, DDS (dietary diversity score), and DVS (dietary variety score) & DVSS (dietary variety score including condiment). In food group intake, the total amount of food intake of the unemployed women (1,554.0 g) were significantly higher than those of the employed (1,477.9 g), while the amount of food from fungi and mushrooms (4.2 g), seaweeds (2.4 g), and fish and shellfish (60.5 g) of the unemployed women was significantly lower than those of the employed. And the amount of meat, poultry and their products (102.8 g), eggs (29.9 g), and animal oil and fats (4.0 g) was significantly higher than those of the employed. Moreover, the common food items of the unemployed women were ranked as eggs (29.7 g), pork (28.9 g), chicken (27.2 g), and beef (26.7 g) at 7, 8, 9, and 10, respectively. When counting the major food groups consumed, DDS=4 has the highest proportion in both groups (unemployed 47.4%, employed 49.1 %). In case of the subjects who had not consumed one particular food group in DDS=4, dairy group was the first (83%), followed by fruit, meat, and vegetables. This order is same in other DDS levels. In dietary variety score (DVS & DVSS), the average number of foods consumed per day of employed women was higher than those of the unemployed. The level of DVSS in the employed women (31.9), especially, is significantly higher than in the unemployed women (30.6). Consequently, highly educated women should pay more attention to increasing dairy intake, and they have need of various foods, over thirty foods without condiments in their diet every day. In addition, encouraging unemployed women to have a nutritionally balanced diet, and offering nutrition education and guidance, such as appropriate choices about animal foods, are needed.

The Study of Relationship among Serum Leptin, Nutritional Status, Serum Glucose and Lipids of Middle-school Girls (일부 사춘기 여중생의 혈청 Leptin 함량과 영양소섭취상태 및 혈당, 혈청지질과의 상관관계 연구)

  • 김미현;승정자
    • Journal of Nutrition and Health
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    • v.33 no.1
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    • pp.49-58
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    • 2000
  • The purpose of this study was to investigate the relationship among urn leptin, nutritional status, and urn lipids, glucose of 99 middle school girls residing in Kyluggi-do, Korea. Subjects were assigned to one of three groups such as underweight(BMI < 20, n=38), normal(20$\leq$BMI<25, n=36) and over weight(BMI$\geq$25, n=25) groups. The nutritional status of the subjects was evaluated by anthropometric measurements, questionnaires, 24-hr dietary recalls and blood anal),sis. The results were as follows : The mean age, body weight, height and BMI were 13.93 years, 55.19kg, 158.40cm and 21.96kg/㎡ respectively. The mean menarche age of the subjects was 12.64 years and the mean daily energy intake was 94.22% off RDA for Koreans. The energy ratio from carbohydrate fat and protein was 63: 24: 13. Underweight group showed the lowest intakes of oils and fats among three groups(p<0.05). The mean serum levels of leptin, glucose, total cholesterol, HDL-cholesterol. LDL-cholesterol and triglyceride were 10.57ng/ml 78.80mg/dl, 12.29mg/dl, 48.07mg/dl, 93.50mg/dl and 78.61mg/d1, respectively. The mean atherogenic index was 2.37. There were significant increases in serum leptin, glucose, LDL-cholesterol, triglyceride and atherogenic index of subjects with increases in their BMIs. Serum leptin showed a significantly positive correlation with BMI(p<0.001), body fat percent(p<0.001). When BMI was adjused, serum leptin showed a significantly positive partial correlations with intakes of animal protein(p<0.05), milks and animal foods(p<0.05), Serum leptin showed a significantly positive correlation with serum glucose(p<0.05) total cholesterol(p<0.01), LDL-cholesterol(p<0.001), triglyceride(p<0.001) and atherogenic index(p<0.001), however, it had significantly negative correlations with HDL-cholesterol(p<0.05). Therefore, these results suggest that the increase of serum levels of leptin may elevate the risk of cardiovascular disease and lipids and some nutrient intakes may be related with serum leptin levels. (Korean J Nutrition 33(1) : 49-58, 2000)

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Improvement of Low Temperature Property of Biodiesel from Palm Oil and Beef Tallow Via Urea Complexation (요소 착물형성에 의한 포화지방산 고함유 팜유 및 우지 유래 바이오디젤의 저온유동성 개선효과 연구)

  • Lee, Yong-Hwa;Shin, Jung-Ah;Zhang, Hua;Lee, Ki-Teak;Kim, Kwang-Soo;Jang, Young-Seok;Park, Kwang-Geun
    • New & Renewable Energy
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    • v.8 no.4
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    • pp.38-43
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    • 2012
  • Biodiesel is non-petroleum based fuel produced from vegetable oils or animal fats through transesterification. The compositions of saturated and unsaturated fatty acids in the feedstocks are important factors for biodiesel quality in terms of low-temperature fluidity and oxidative stability. The goal of this study is to improve the cold flow property of biodiesel from vegetable and animal origin containing highly saturated methyl esters (approx. 50%). In this purpose poly-saturated methyl esters in palm and tallow biodiesel were removed via urea-based fractionation and then the recovered fractions (enriched unsaturated fatty acid methyl esters) were supplemented with cold flow improvers. The highest concentration of unsaturated fatty acid methyl esters (93.8%) was obtained using a urea/fatty acid ratio of 3:1 at the crystallization temperature of $0^{\circ}C$ for 17 hours in incubation, with recovery of 71% and the addition of cold flow improver (Flozol$^{(R)}$ 515, 3,000 ppm) to the enriched poly-unsaturated fatty acid methyl esters reduced the CFPP(cold filter plugging point) of palm biodiesel from $12^{\circ}C$ to $-42^{\circ}C$. In tallow biodiesel both the enrichment of unsaturated fatty acid methyl esters (93.71%) and the addition of cold flow improver (Infineum R408, 3,000ppm) reduced the CFPP from $10^{\circ}C$ to $-32^{\circ}C$.

A Study on Dietary Factors Related to the Incidence of Stomach Cancer and Colon Cancer in Korea (위암 및 대장암의 유발에 영향을 미치는 식이요인에 관한 연구)

  • 양은주
    • Journal of Nutrition and Health
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    • v.26 no.5
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    • pp.603-614
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    • 1993
  • This study was undertaken to investigate the dietary factors related to the incidence of stomach and colon cancers in Korean. The subjects were 139 stomach and 52 colon cancer patients recruited from 3 general hospitals in Seoul. Food intake, anthropometric measurement, and blood compositions were studied through personal interview and using medical records. Body weight, body mass index, triceps skinfold thickness, body muscle mass of the subjects were lower than reference values. The body weight was reduced after the onset of the illness, which suggests body waste due to the cancers. The patients showed the lower valuies of hemoglobin and hematocrit. Serum protein and calcium were at lower limit of the normal range. Therefore the untritional status assessed by anthropometry and blood composition should be said to be marginal. The average intake of most of the nutrients except Ca of the subjects met the RDA, but the range was wide and the nutrient intake of large proportion of the subjects feel below 75% of RDA. The food intake of egg, milk, legumes, and fruts were lower than national average, on the other hand the subjects had higher intake of meat, vegetables, and fats. The subjects drank and smoked heavily, stomach cancers being more severs. From this results, dietary risk factors for the stomach and colon cancers in Korean did not agree with the reports of western societies. Even though the intakes of meat and animal food of colon cancer patients can not be classified as high, it was higher than stomach cancer and national average. Therefore it could be concluded that with increasing consumption of animal food, the incidence of colon cancer would be increased in Korea.

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Food behaviors accounting for the recent trends in dietary fatty acid profiles among Korean adults

  • Song, SuJin;Shim, Jae Eun
    • Nutrition Research and Practice
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    • v.16 no.3
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    • pp.405-417
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    • 2022
  • BACKGROUND/OBJECTIVES: This study examined the changes in food behaviors of dietary fatty acids over 2007-2018 among Korean adults. SUBJECTS/METHODS: This study used data from the 4th (2007-2009), 5th (2010-2012), 6th (2013-2015), and 7th (2016-2018) Korea National Health and Nutrition Examination Surveys. A total of 46,307 adults aged 19-64 yrs were selected and dietary data were obtained from a single 24-h recall. In the 4th and 7th data, the major food sources for each fatty acid based on the contributing percentage of the food item were compared. The consumption trends in the major food sources were presented as grams per day over 2007-2018 and compared across the survey periods using the multiple regression model. RESULTS: From 2007 to 2018, for total fat, saturated fatty acid (SFA), and monounsaturated fatty acid, the contribution of animal food sources, including beef, chicken, and eggs increased but plant food sources (e.g., tofu, soybean, and plant oil) decreased. As polyunsaturated fatty acid sources, mayonnaise, eggs, and bread showed higher contributions, whereas soybean and tofu showed lower contributions in the 7th data compared to the 4th data. For n-3 fatty acids, the contribution of fish decreased between the 4th and 7th data. Over 12 yrs, the significant increases in the absolute amount of consumption from animal sources were observed. In contrast, decreases in the consumption from plant sources and fish were seen across the survey periods. CONCLUSIONS: In Korean adults, increases in the intake of dietary fatty acids along with changes in the food behaviors during 2007-2018 have evoked great concern for SFA intake, which is a cardiovascular disease risk factor. Healthy food sources of dietary fatty acids should be emphasized in this population.

Effects of Rare Earth Supplementation on Growth Performance, Blood Immune-Related Cell Population, Meat Quality and Fecal Odor Emission Gases in Finishing Pigs (사료내 희토 첨가가 비육돈의 생산성, 혈액내 면역관련 세포수, 육질특성 및 분 중 악취방출 가스함량에 미치는 영향)

  • Shin, Seung-Oh;Yoo, Jong0Sang;Lee, Je0Hyun;Jang, Hae0Dong;Kim, Hyo0Jin;Huang, Yan;Chen, Ying-Jie;Cho, Jin-Ho;Kim, In-Ho
    • Journal of Animal Science and Technology
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    • v.50 no.4
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    • pp.485-498
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    • 2008
  • This study was conducted to evaluate effects of dietary rare earth on growth performance, blood immune- related cell population, meat quality and fecal odor emitting gases in finishing pigs. The total of sixty four (Landrace×Yorkshire×Duroc) pigs(65.42±1.16kg in average initial body weight) were used for feeding trial during 10 weeks of experimental period. Dietary treatments included 1) NC(antibiotic free diet), 2) PC (NC diet+6 weeks 44ppm of tylosin/ 4 weeks 22ppm of tylosin) 3) RE1 (NC diet + 100ppm of RE), 4) RE2 (NC diet+200ppm of RE). There were four dietary treatments with four replicate pens per treatment and four pigs per pen. During the overall periods, there were no significant differences in ADG(Average daily gain), ADFI (Average daily feed intake) and gain/feed ratio among treatments(P>0.05). Dry matter and nitrogen digestibility were higher in RE2 treatment group than other groups(P<0.05). Also, energy digestibility was higher in RE2 treatment group than PC and RE1 treatment groups(P<0.05). At the 6th week WBC(white blood cell) was significantly increased(P<0.05) in RE1 treatment group than NC and RE2 treatment groups. L* value of M. logissimus dorsi muscle color was significantly increased(P<0.05) in rare earth supplemented groups compared to NC treatment group(P<0.05). However, a* value was lower in RE1 treatment group than PC treatment group (P<0.05). In fatty acid composition of Intramuscular fat, total MUFA was significantly higher in RE2 treatment group than other groups(P<0.05). Also, total UFA was significantly increased in RE2 treatment group compared with NC and PC treatment groups(P<0.05). In fatty acid composition of back fats, total SFA of rare earth supplemented groups were lower than in PC treatment group(P<0.05). UFA:SFA ratio was significantly higher in rare earth supplemented groups than PC treatment group(P<0.05). In fecal odor emission, NH3 was significantly decreased(P<0.05) in rare earth supplemented groups compared to NC and PC treatment groups. In conclusion, the results of the experiment was affected by rare earth supplementation on digestibilities, meat quality, fatty acid and fecal odor emission gases in finishing pigs.