• 제목/요약/키워드: Anguilla japonica

검색결과 196건 처리시간 0.027초

뱀장어 치어의 사료내 에너지에 대한 적정 단백질 비 평가 (Evaluation of the Optimum Dietary Protein to Energy Ratio of Juvenile Japanese eel, Anguilla japonica)

  • 한경민;배준영;;고수홍;유진형;배승철
    • 한국양식학회지
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    • 제18권3호
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    • pp.135-141
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    • 2005
  • 본 실험은 치어기 Japanese eel (A. japonica)의 에너지 수준에 대한 사료내 적정 단백질 비를 평가하기 위하여 갈색어분(Brownfish meal)과 혈분(Blood meal)을 사용하여 조단백질 함량은 에너지에 대해 각각 45%와 50%,그리고 총에너지 함량은 각각 3800, 4150, 4500 kcal/kg의 반정제사료(semipurified)를 제조하여 16주간 사육실험을 실시하였다. 본 연구는 사료내 에너지 수준에 따라 단백질 함량을 달리 조절하여 사육실험을 실시하여 에너지 수준에 따른 적정 단백질 함량을 규명하는데, %증체율(WG), 일간성장률(SGR) 등의 성장평가와 사료효율(FE), 단백질효율(PER) 등의 사료영양학적 평가 및 혈액성상학적 평가와 연관해서 수행하였으며 실험 종료 후 어체내 일반성분 및 혈청 내 생화학적 조성을 비교 조사하였다. WG과 SCR에 있어서 $_{100}P_{45}$실험구가 다른 실험구들에 비해서 유의적으로 높게 나타났다(P<0.05). FE에 있어서 $_{100}P_{45}$실험구가 $_{100}P_{45}$ 실험구를 제외한 다른 실험구들에 비해서 유의하게 높은 것으로 나타났으며(P<0.05), SGR 역시 $_{100}P_{45}$ 실험구가 다른 실험구들에 비하여 유의적으로 높게 나타는데 $_{100}P_{45}$ 실험구와는 유의적인 차이가 없었으며, 이들을 제외한 나머지 실험구들간에 있어서 유의한 차이는 나타나지 않았다(P<0.05). 따라서, 본 결과를 통하여 치어기 Japanese eel (A. japonica)에 있어서 사료내 단백질 함량 45%, 에너지 4500 kcal/g가 최적의 성장을 나타낼 것으로 예측되며, 이때 단백질과 에너지 비율(P/E ratio)은 100mg protein/kcal가 적합한 것으로 판단된다.

새만금 일대의 어류상 (Fish Fauna of the Saemankum Area in the West Coast of Chollabuk-do, Korea)

  • 심광수;이충렬
    • 환경생물
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    • 제17권3호
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    • pp.293-303
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    • 1999
  • 전라북도 서해에 위치하고 있는 새만금 지역 일대에 서식하고 있는 어류에 대한 조사가 1996년 10월부터 1999년 4월까지 5개 지점을 중심으로 실시되었다. 그 결과 본 구역에서 서식이 확인된 어류는 총 13목 52과 83속 107종이었다. 본 수역에서 출현하는 어종 중에서 농어목 어류가 총 50종으로 전체 어류의 46.7%를 나타내었고, 다음이 16종인 횟대목과 14종인 가자미목 순으로 나타나 이들 3목 어류가 전체 출현 어종의 74.8%를 차지하고 있었다. 한편 이들 어류 중에서 13종이 확인된 망둑어과 어류는 12.1%로 가장 다양한 종이 출현하였고, 다음이 민어과 어류가 6종으로 5.6%, 그리고 멸치과와 참서대과 어류가 각각 5종씩으로 4.7%씩 나타내었다. 한편 본 수역에서 가장 많은 개체수가 출현하여 이 지역 일대의 우점종으로 나타난 종은 E. japonicus로 전체의 23.4%였고, 다음은 T. kanmalensis가 18.5%, S. schlegeli가 12.3%, S. zunasi가 11.1% 등의 순으로 나타나, 멸치과와 청어과 어류가 전체 어류의 61.7%로써 대부분을 차지하고 있었다. 또한 본 조사 지역에서 출현량도 많고, 경제적으로 중요한 어종으로는 A. japonica, E. japonicus, T kammalensis, K. punctatus, S. zunasi, M. cephalus, S. schlegeli, A. argentatus, P polyactis, C. lucidus, C. joyneri등이고,계절적으로 우기가 들기 전에 출현량이 많은 어종으로는 A. japonica, C. nasus, E. japonicus, T. kummulensis, K. punctatus, S. zunasi, S. schlegeli, P. polyactis, C. lusidus, P. fangi등이었으나 해수의 염도가 낮은 우기철에는 거의 출현하지 않았다. 한편 본 수역에서 출현하는 어종 중에서 우리나라의 고유어종으로는 O. rotundus, R. koreanus와 R. leucopoecilus등의 3종이었다. 본 새만금 사업이 진행됨으로써 본 지역에서 완전 소멸될 수 있는 종으로는 B. pectinirostris와 갯벌에서만 서식하는 O. rotundus가 제일 먼저 영향을 받을 것으로 생각되며, 그외에도 출현하는 개체수가 급격히 감소하거나 서식에 어려움을 줄 수 있는 종으로는 R. leucopoecilus, P. modestus, T. barbatus, L. ocellicauda등이다. 이 뿐만 아니라 우리나라에서 가장 넓은 갯벌이 소멸됨으로 인해 전라북도 서해연안에 물리적, 생태적인 악영향이 크게 나타날 것으로 사료되며, 특히 광활한 연안 조간대가 없어짐으로 인해 우기철의 만조시기에 인근 저지대에서 침수의 재해가 발생할 가능성도 있다.

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Assessment of Neuronal Cell-Based Cytotoxicity of Neurotoxins from an Estuarine Nemertean in the Han River Estuary

  • Kwon, Yeo Seon;Min, Seul Ki;Yeon, Seung Ju;Hwang, Jin ha;Hong, Jae-Sang;Shin, Hwa Sung
    • Journal of Microbiology and Biotechnology
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    • 제27권4호
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    • pp.725-730
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    • 2017
  • A heteronemertean, Yininemertes pratensis, was collected in Han River Estuary, South Korea. This estuarine nemertean has been known by the local fishermen for harmful effects to the glass eels, juveniles of Japanese eel Anguilla japonica, migrating to fresh water. The present study confirmed the neurotoxic effects of this heteronemertean ribbon worm at the cellular level. Derivative types of neurotoxic tetrodotoxin (TTX), 5,11-dideoxy TTX (m/z 288) and 11-norTTX-6(S)-01 (m/z 305.97), were identified through HPLC and MALDI-TOF MS. However, significant neurotoxicity was confirmed in the fraction containing an undefined molecule corresponding to the 291.1 (m/z) peak, when tested in rat primary astrocytes and dorsal ganglion cells. This study is the first to report neurotoxins of the estuarine nemertean, fairly abundant in the Han River estuary, and suggests the long-term monitoring of population dynamics and surveillance of the toxicity in this river estuary.

양식 어류와 이들 난에 대한 항곰팡이성 약물들의 효과 비교 (Comparative Efficacy of Antifungal Agents for Aquaculture Fish and their Eggs)

  • 지보영;이덕찬
    • 한국수산과학회지
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    • 제42권1호
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    • pp.34-40
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    • 2009
  • In fresh water fish hatcheries and farms, Saprolegniales often cause serious mortality to the fish and their eggs. Malachite green is an effective antifungal agent, but is carcinogenic to fish and humans. Alternative antifungal agents are needed. Presently, we tested various concentrations of MBT-01108 (Opuntia ficus-indica extracts) alone and in combination with bronopol, formalin and sodium chloride (MBT-01108 mixture) on in vitro mycelial growth and in vivo remediation of adult eel, Anguilla japonica, infected with Saprolegnia sp. and fertilized eggs of chum salmon, Oncorhynchus keta, to evaluate the compounds' antifungal efficacy on eyed-egg and hatching rates. MBT-Oll08 mixtures incorporating bronopol and formalin at respective concentrations of 50 and 30 parts per million (ppm), and 100 and 20 ppm were most effective in controlling Saprolegnia in vitro and in vivo (P<0.05). Repeated daily exposures to 50 ppm and 100 ppm MBT-01108 were more effective than exposure every 2-3 days post-fertilization for the inhibition of Saprolegnia infection of rainbow trout, Oncorhynchus mykiss eggs as compared with control (0 ppm).

Effect of TBT and PAHs on CYP1A, AhR and Vitellogenin Gene Expression in the Japanese Eel, Anguilla japonica

  • Choi, Min Seop;Kwon, Se Ryun;Choi, Seong Hee;Kwon, Hyuk Chu
    • 한국발생생물학회지:발생과생식
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    • 제16권4호
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    • pp.289-294
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    • 2012
  • Gene expressions of cytochrome P4501A (CYP1A), aryl hydrocarbon receptor (AhR) and vitellogenin (Vg) by endocrine disruptors, benzo[${\alpha}$]pyrene (B[a]P) and tributyltin (TBT) were examined in cultured eel hepatocytes which were isolated from eels treated previously with B[a]P (10 mg/kg) or estradiol-$17{\beta}$ (20 mg/kg) in vivo, and the relationship between CYP1A, AhR and Vg genes were studied. When the cultured eel hepatocytes were treated with B[a]P ($10^{-6}-10^{-5}M$) the gene expressions of CYP1A and AhR were enhanced in a concentration-dependent manner. However, when treated with TBT ($10^{-9}-10^{-5}M$) the gene expressions of CYP1A and AhR were suppressed at high concentrations ($10^{-6}-10^{-5}M$), while having no effects at low concentrations ($10^{-9}-10^{-7}M$). Gene expression of Vg was also suppressed by TBT in a concentration-dependent manner in cultured eel hepatocytes which was previously treated in vivo with estradiol-$17{\beta}$.

양식어류에 있어서 생약제제(바이오감마믹스)의 경구투여에 의한 효능 및 안전성에 관한 연구 (A study on efficacy and safety of Bio-Gammamix by oral administratiion to cultured fish)

  • 허강준;김병기;예제길;김정호
    • 대한수의학회지
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    • 제34권3호
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    • pp.627-633
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    • 1994
  • A study on efficacy and safety of Bio-Gammamix was performed to use for the preventive drug against bacterial diseases to mirror carp(Cyprinus carpio), rainbow trout(Salmo gairdneri), eel(Anguilla japonica) and flounder(Paralichthys olivaceus). The main ingredients of Bio-Gammamix were ferrous fumarate and mixed extrated powders of licorice root, ox bile and garlic. The test drug was proved to improve feeding habit and feed efficiency and decrease cumulative mortality remarkably. After administration, no side effects and histopathological changes were observed. We can prevent outbreaks of bacterial diseases of fish by the dosage of 1kg/ton of fish body weight perorally once or two times in a day for 7 days and Bio-Gammamix is thought to be used effectively an widely against most bacterial fish pathogens.

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천연항균성 물질을 이용한 Vibrio vulnificus 의 살균 및 독소생성 억제효과 (disinfectant and Inhibitory Effect of Natural Antimicrobial Agent on Vibrio vulnificus in Fish)

  • 조성환;서일원;최종덕;전상수;라택균;정수근;강동훈
    • 한국식품위생안전성학회지
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    • 제7권2호
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    • pp.99-106
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    • 1992
  • To prevent food-poisoning outbreaks by Vibrio vulnificus the antimicrobial efficacy of grapefruit seed extract (GFSE) was examined. Minimal inhibitory concentration (in vitro) for the microorganism was found to be 50∼100 ppm. Transmission electron micrographs of Vibrio vulnificus showed the biocidal action of this natural antimicrobial agent would be related to specific respiratory effect coupled with the destruction of permeable function of microbial cell membrane. After Anguilla japonica GFSE-injected to the body was incubated in the seawater contaminated by Vibrio vulnifiucs the fish meats were taken up, mixed with control diet and used as a diet in the feeding experiment. In this experiment the effect of GFSE treated with fish muscle on body weight, protein efficiency ratio, serum enzymes and serum blood components of broiler chicks was investigated. It is proved from this study that there is neither Vibriosis nor toxicity associated with GFSE itself an fish meats treated with it when it is injected to the fish body at a level of 250 ppm or less.

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탐진강 수계의 어류상에 관하여 (On the Ichthyofauna of the Tamjin River System)

  • 황영진;최충길
    • 한국어류학회지
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    • 제7권2호
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    • pp.135-139
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    • 1995
  • 탐진강 수계에서 채집된 어류는 총 10과 29속 39종이었으며 고유종은 12종이었고 잉어과에 해당하는 어류는 14속 21종으로 다양하게 나타났다. 전 조사지점에서 채집된 어류는 Puntungia herzi 뿐이었고 Rhodeus uyekii, Acheilognathus koreanus, Squalidus gracilis majimae, Microphysogobio yaluensis, Zacco platypus, Zacco temmincki, Cobitis taenia lutheri, Cobitis longicorpus, Odontobutis platycephala가 광범위하게 분포하고 있는 것으로 나타났다. 비교적 우세하게 출현하는 종은 Zacco temmincki, Acanthorhodeus gracilis, Zacco platypus, Rhodeus uyekii, Puntungia herzi, Squalidus gracilis majimae이었다. 반면 희소하게 출현된 종들은 Anguilla japonica, Rhodeus ocellatus, Pseudorasbora parva, Abbottina rivularis, Silurus asotus 등이었다. Odontobutis obscura interrupta가 본 수계에서 처음으로 채집되어 주목되었다.

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양식 어류에 있어서 황산동(copper sulfate pentahydrate)의 안전성과 살균효과에 대한 연구 (Antibacterial efficacy and safety of copper sulfate pentahydrate to cultured fish)

  • 허강준
    • 대한수의학회지
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    • 제37권1호
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    • pp.203-212
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    • 1997
  • Antibacterial efficacy and safety of copper sulfate pentahydrate (5% solution) was evaluated in laboratory and field studies for disinfection of some fish bacterial pathogens in cultured fish, flounder(Paralichthys olivaceus), mirror carp(Cyprinus carpio), gold fish(Carrassius auratus) and eel(Anguilla japonica). Dipping of fishes for acute toxicity was performed for a period of 48h, and the TLm value(median tolerance limit) were 4.0ppm in mirror carp and flounder, 4.4ppm in rainbow trout, and 10.0ppm in gold fish. After exposure below than TLm value for 1 month, no side effects and histopathological changes were observed. The test drug were shown high antibacterial activities against most bacterial pathogens by exposure in the laboratory and field trials. However, Aeromonas salmonicida showed a resistance to the dosage. We can prevent outbreaks of bacterial diseases of fish by the dosage of 1ppm for 6hr and the test drug is thought to be used effectively and widely as a disinfectant against most bacterial fish pathogens.

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간세포 배양을 이용한 뱀장어 Vitellogenin 합성에 대한 웅성호르몬의 영향 (Induction of Vitellogenin Synthesis by Androgens in Cultured Hepatocytes of the Eel, Anguilla japonica)

  • 권혁추;박홍양
    • 한국가축번식학회지
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    • 제20권3호
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    • pp.259-269
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    • 1996
  • To establish whether or not androgens is responsible for the induction of vitellogenin(Vg) synthesis and secretion, primary hepatocytes prepared from immature eels were used. The results are follows: 1. Eel hepatocytes were prepared using a collagenase perfusion technique. The isolated cells attached efficiently to fibronectin-coated dishes and subsequently formed monolayers in serum-free medium. These cultures maintained in medium for 10 days with minimal cell loss. 2. Estradiol-17$\beta$(E2) alone was insufficient to induce Vg synthesis. The combination of E2 with methyltestosterone(MT) markedly stimulated Vg synthesis. High vg production occurred in MT concentration from 10-6~10-5M in the presence of E2 (10-6M). Testosterone and androsterone were also effective, but progesterone was not effective in inducing Vg synthesis. Neither MT alone nor testosterone and androsterone alone had any effect on Vg synthesis. 3. E2-primed hepatocytes showed Vg synthesis in both media with and without hormones 1 day after culture. In the cultures with the vehicle, MT, or progesterone, the rate of synthesis seemed to decrease with time. But the combination of E2 and MT showed an intense increase in Vg synthesis. Hepatocytes isolated from E2-primed eels also required androgens for continuating of Vg synthesis. 4. These results demonstrate that androgens act together with E2 in synthesis and secretion of eel Vg.

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