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http://dx.doi.org/10.5657/kfas.2009.42.1.034

Comparative Efficacy of Antifungal Agents for Aquaculture Fish and their Eggs  

Lee, Bo-Young (Pathology Division, National Fisheries Research & Development Institute)
Lee, Deok-Chan (Pathology Division, National Fisheries Research & Development Institute)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.42, no.1, 2009 , pp. 34-40 More about this Journal
Abstract
In fresh water fish hatcheries and farms, Saprolegniales often cause serious mortality to the fish and their eggs. Malachite green is an effective antifungal agent, but is carcinogenic to fish and humans. Alternative antifungal agents are needed. Presently, we tested various concentrations of MBT-01108 (Opuntia ficus-indica extracts) alone and in combination with bronopol, formalin and sodium chloride (MBT-01108 mixture) on in vitro mycelial growth and in vivo remediation of adult eel, Anguilla japonica, infected with Saprolegnia sp. and fertilized eggs of chum salmon, Oncorhynchus keta, to evaluate the compounds' antifungal efficacy on eyed-egg and hatching rates. MBT-Oll08 mixtures incorporating bronopol and formalin at respective concentrations of 50 and 30 parts per million (ppm), and 100 and 20 ppm were most effective in controlling Saprolegnia in vitro and in vivo (P<0.05). Repeated daily exposures to 50 ppm and 100 ppm MBT-01108 were more effective than exposure every 2-3 days post-fertilization for the inhibition of Saprolegnia infection of rainbow trout, Oncorhynchus mykiss eggs as compared with control (0 ppm).
Keywords
Antifungal agent; Fungal disease; MBT-01108; Saprolegnia parasitica;
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1 Allen, J.L., J.E. Gofus and J.R. Meinertz. 1994. Determination of malachite green residues in the eggs, fry, and adult muscle-tissue of rainbow-trout (Oncorhynchus mykiss). J. Assoc. Offic. Anal. Chemist. Int., 77, 553-557   DOI   ScienceOn
2 Aller-Gancedo, J.M. and J.M. Fregeneda-Grandes. 2007. Comparative efficacy of Pyceze${\circledR}$ (bronopol) in controlling mortality of brown trout Salmo trutta eggs. Aquaculture Res., 38, 618-624   DOI   ScienceOn
3 Bly, J.E., S.M.A. Quiniou, L.A. Lawson and L.W. Clem. 1996. Therapeutic and prophylactic measures for winter saprolegniosis in channel catfish. Dis. Aquat. Org., 24, 25-33   DOI
4 Cerenius, L. and K. Soderhall. 1992. Crayfish diseases and crayfish as vectors for important diseases. Finfish Fish. Res., 14, 125-133   DOI   ScienceOn
5 Kiesecker, J.M., A.R. Blaustein and L.K. Belden. 2001. Complex causes of amphibian population declines. Nature, 410, 681-684   DOI   ScienceOn
6 Marking, L.L., J.J. Rach and T.M. Schreier. 1994. Evaluation of antifungal agents for fish culture. Prog. Fish-Cult., 56, 225-231   DOI   ScienceOn
7 Oono, H., K. Hatai, M. Miura, N. Tuchida and T. Kiryu. 2007. The use of bronopol to control fungal infection in rainbow trout eggs. Biocontrol. Sci., 12, 55-57   DOI   PUBMED   ScienceOn
8 Treasurer, J.W., E. Cochrane and A. Grant. 2005. Surface disinfection of cod Gadus morhua and haddock Melanogrammus aeglefinus eggs with Bronopol. Aquaculture, 250, 27-35   DOI   ScienceOn
9 Willoughby, L.G. 1978. Saprolegniasis of salmonid fish in Windermere: A critical analysis. J. Fish Dis., 1, 51-67   DOI
10 Willoughby, L.G. and A.D. Pickering. 1977. Viable Saprolegniaceae spores on the epidermis of the sa1monid fish Salmo trutta and Salmo alpinus. Trans. Br. Mycol. Soc., 68, 91-95   DOI
11 Yanong, R.P. 2003. Fungal diseases of fish. Vet. Clin. North. Am. Exot. Anim. Pract., 6, 377-400   DOI   ScienceOn
12 Kim, H.N., D.H. Kwon, H.Y. Kim and H.K. Jun. 2005. Antimicrobial activities of Opuntia ficus-indica var. saboten Makino methanol extract. J. Life Sci., 15, 279-286   DOI   ScienceOn
13 Meyer, F.P. and T.A. Jorgenson. 1983. Teratological and other effects of malachite green on development of rainbow trout and rabbits. Trans. Am. Fish. Soc., 112, 818-824   DOI
14 Ali, E.H. 2005. Morphological and biochemical alterations of oomycete fish pathogen Saprolegnia parasitica as affected by salinity, ascorbic acid and their synergistic action. Mycopathologia, 159, 231-243   DOI   PUBMED   ScienceOn
15 Gieseker, C.M., S.G. Serfling and R. Reimschuessel. 2006. Formalin treatment to reduce mortality associated with Saprolegnia parasitica in rainbow trout, Oncorhynchus mykiss. Aquaculture, 253, 120-129   DOI   ScienceOn
16 Jee, B.Y., D.C. Lee, N.Y. Kim, S.H. Jung and S.I. Park. 2007. Identification and chemotherapeutic effects of the fungi from three salmonid species and their eggs. J. Fish Pathol., 20, 147-160
17 FDA (Food and Drug Administration). 2002. Part C, Enforcement Priorities. In Enforcement priorities for drug use in aquaculture USA. http://www.fda.gov/ cvm/index/policy_proced/4200.pdf
18 Willoughby, L.G. 1985. Rapid preliminary screening of Saprolegnia on fish. J. Fish Dis., 8, 473-476   DOI
19 Aldennan, D.J. 1985. Malachite green: a review. J. Fish Dis., 8, 289-298   DOI   ScienceOn
20 Hussein, M.M., K. Hatai and T. Nainura. 2001. Sapro-legniasis in sa1minides and fishes their eggs in Japan. J. Wildl. Dis., 37, 204-207   DOI   PUBMED
21 Seymour, R.L. 1970. The genus Saprolegnia. Nova Hedwigia (Beih.), 19, 1-124
22 Khodabandeh, S. and B. Abtahi. 2006. Effects of sodium chloride, formalin and iodine on the hatching success of common carp, Cyprinus carpio, eggs. J. Appl. Ichthyol., 22, 54-56   DOI   ScienceOn
23 Gaikowski, M.P., J.J. Rach and R. Ramsay. 1999. Acute toxicity of hydrogen peroxide treatments to selected lifestages of cold-, cool-, and warmwater fish. Aquaculture, 178, 191-207   DOI   ScienceOn
24 Pickering, A.D., L.G. Willoughby and C.B. McGrory. 1979. Fine structure of secondary zoospore cyst cases of Saprolegnia isolates from infected fish. Trans. Br. Mycol. Soc., 72, 427-436   DOI
25 Hatai, K., L.G. Willoughby and G.W. Beakes. 1990. Some characteristic of Saprolegnia obtained from fish hatcheries in Japan. Mycol. Res., 94, 182-190   DOI
26 Doerge, D.R., M.l. Churchwell, T.A. Gehring, M.Y. Pu, and S.M. Plakas. 1998. Analysis of malachite green and metabolite in fish using liquid chromatography atmospheric pressure chemical ionization mass spectrometry. Chern. Res. Toxicol., 11, 1098-1104   DOI   ScienceOn
27 Bruno, D.W. and B.P. Wood. 1999. Saprolegnia and other oomycetes. In: Fish Diseases and Disorders. Wood, P.T.K. and Bruno, D.W .. eds. Wallingford, UK, CAB International, 3, 599-659
28 Dieguez-Uribeondo, J., J.M. Fregeneda-Grandes, L. Cerenius, E. Perez-Iniesta, J.M. Aller-Gancedo, M.T. Telleria, K. Soderhall and M.P. Martin. 2007. Re-evaluation of the enigmatic species complex Saprolegnia diclina-Saprolegnia parasitica based on morphological, physiological and molecular data. Fungal Gen. BioI., 44, 585-60l   DOI
29 Standing Committee on the Food Chain and Animal Health. 1990. http://ec.europa.eu/food/food/chemicalsafety/ residues/coun Jeg_ 2377 _90 _en. pdf
30 Hatai, K. and G.I. Hoshinai. 1992. Saprolegniasis in cultured coho salmon (Oncorhynchus kisutch). Fish Pathol., 27, 233-234   DOI   ScienceOn
31 Noga, EJ. 1993. Water mold infections of freshwater fish: Recent advances. In: Annual Review of Fish Diseases, Pergamon Press Ltd., 291-304
32 Bly, J.E., L.A. Lawson, D.J. Dale, A.J. Szalai, R.M. Durborow and L.W. Clem. 1992. Winter saprolegniosis in channel catfish. Dis. Aquat. Org., 13, 155-164   DOI
33 Arndt, R.E., E.J. Wagner and M.D. Routledge. 2001. Reducing or withholding Hydrogen peroxide treat-ment during a critical stage of rainbow trout development: Effects on eyed eggs, hatch, defonnities and fungal control. N. Am. J. Aquatcul., 63, 161-166   DOI   ScienceOn
34 Meinertz, J.R., G.R. Stehly, W.H. Gingerich and J.L. Allen. 1995. Residues of $[^{14}C]$-malachite green in eggs and fry of rainbow trout, Oncorhynchus mykiss (Walbaum) after treatement of eggs. J. Fish Dis., 18, 239-147   DOI   ScienceOn
35 Yuasa, K. and K. Hatai. 1995. Relationship between pathogenicity of Saprolegnia spp. isolation to rainbow trout and their biological characteristics. Fish Pathol., 30, 101-106   DOI   ScienceOn
36 Dieguez-Uribeondo, J., L. Cerenius and K. Soderhall. 1994. Saprolegnia parasitica and its virulence on three different species of freshwater crayfish. Aquaculture, 120, 219-222   DOI   ScienceOn
37 Espeland, S. and P.E. Hansen. 2004. Prevention of Saprolegnia on rainbow trout eggs. BSc thesis, University of the Faroe Islands, Torshavn, Faroe Islands
38 Aldennan, D.1. 1982. In vitro testing of fisheries chemotherapeutants. J. Fish Dis., 5, 113-123   DOI