• 한방안이비인후피부과학회지
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• 제18권1호
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• pp.1-12
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• 2005

Melanin determines phenotypic appearance and its election-opaque property protects cells from physical, including ultraviolet (UV) radiation, and chemical stimuli such as free radicals. However hyper-pigmentation is associated with various skin diseases such as keloid scar. The aim of present study was to investigate the effects of aqueous extracts from Angelica dahurica Benth. (AEAD) on ${\alpha}-Melanocyte$ stimulating hormone $({\alpha}-MSH)-induced$ melanogenesis in B16 mouse melanoma cell. Relative high doses ($5\;mg/m{\ell}$) of AEAD could inhibit melanin formation without apoptotic death in cells treated with ${\alpha}-MSH$. And also, ${\alpha}-MSH-induced$ activation of tyrosinase was inhibited in cells treated with AEAD. These results suggest that AEAD inhibit melanogenesis through inhibiting tyrosinase activity, and also, AEAD may apply to develop whitening drugs and cosmetics.

• 생약학회지
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• 제21권2호
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• pp.121-125
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• 1990

Essential oil of the root of Angelica dahurica Benth et Hook(Umbelliferae) was investigated. Essential oil was obtained from the dried roots by steam distillation and fractionated by column chromatography. Each isolate or fraction was identified by GC, GC-MS and spectral analysis. It was found to contain eleven monoterpenes such as ${\alpha}-pinene(4.74%),\;campben, {\beta}-pinene,\;myrcene,\;{\alpha}-phellandrene,\;{\delta}-3-carene(39.4%),\;{\alpha}-terpinene,\;{\rho}-cymene,\;{\beta}-phellandrene,\;{\alpha}-terpinene,\;terpinolene\;and\;also\;found\;to\;contain\;4-vinylguaiacol,\;iso-elemicin,\;{\beta}-elemene$, caryophyllene, ligustilide, osthol and seven tentatively identified sesquiterpenes.

• 생약학회지
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• 제21권3호
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• pp.239-241
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• 1990

A new method for quantitative determination of byakangelicin in Angelicae dahuricae Radix by high performance liquid chromatography was established. A reversed-phase system with a ${\mu}Bondapak$ $C_{18}$ column using THF : dioxane : MeOH : HAc : 5% $H_3PO_4$ : $H_2O$=72.5 : 62.5 : 25 : 10 : 1 : 329 as a mobile phase was developed. Byakangelicin together with ter-O-byakangelicin and oxypeucedanin methanolate, and isooxypeucedanin as an internal reference were detected at 350 nm and the analysis was successfully carried out within 30 min.

• 동의생리병리학회지
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• 제18권6호
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• pp.1896-1903
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• 2004

Anglicae dahuricae Radix (ADR), the dried roots of Angelica Dahurica Benth et Hook (Umbelliferae), is a traditional herbal medicine used to treat colds, headache, skin diseases such as acne and possess analgesic, antipyretic and drainage effects. In the present study, the author evaluated the effect of ADR on regulation of inflammatory reaction. ADR reduced the ear-swelling responses derived from compound 48/80 in dose-dependent manner significantly. ADR inhibited the PMA plus A23187-induced productions of IL (Interleukin)-8, IL-1β, granulocyte macrophage-colony stimulating factor (GM-CSF) and tumor necrosis factor (TNF)-α from human mast cells (HMC)-1. In addition, ADR blocked PMA plus A23187-induced ERK1/2 phosphorylation. Ⅰ suggest that ADR regulates inflammatory reaction through inhibition of inflammatory cytokines such as IL-8, IL-1β and GM-CSF.

• 현장농수산연구지
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• 제24권4호
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• pp.18-29
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• 2022

청정지역 지리산에서 자생하는 5종(누리장나무, 구릿대, 층꽃나무, 인동덩굴, 박쥐나물)의 식물을 선정하여 5월부터 9월까지 자생지에서 꽃이 활짝 개화한 상태에서 채집하였다. 채집한 식물은 전초는 추출하여 17종의 폴리페놀 성분을 분석하고, 다음으로 꽃, 잎, 줄기, 뿌리를 분리하여 각 부위의 추출물로 DPPH, ABTS, 폴리페놀, 플라보노이드에 관한 실험을 진행한 결과는 다음과 같다. 1. 지리산에 자생하는 5종의 식물에 함유된 폴리페놀 성분은 총 8가지였으며, 그중 가장 많은 것은 chlorogenic acid(4종)였고, 다음으로 caffeic acid(2종)가 많았다. 2. DPPH radical 소거 활성을 살펴본 결과 박쥐나물 줄기가 가장 좋았으며, 누리장나무는 특정 부위와 상관없이 좋았으며, 구릿대는 꽃, 인동덩굴에서는 잎에서 활성소거능이 좋았다. 3. ABTS radical 소거 활성도가 가장 높은 것은 누리장나무로 EC₅₀ 값은 38.73~66.28ppm으로 나타났고, 다음으로는 인동덩굴이었으며, 구릿대와 박쥐나물의 잎과 줄기, 층꽃나무 잎과 줄기 순이었다. 4. 총폴리페놀 함량이 가장 많은 것은 누리장나무 잎의 154.83 mgGAE/g이었고, 다음으로 누리장나무 꽃과 박쥐나물 줄기로 약 130 mgGAE/g이었다. 가장 낮은 것은 구릿대 줄기로 26.27 mgGAE/g인 것으로 나타났다. 5. 총플라보노이드 함량은 박쥐나물 가지는 256.34 mgGAE/g으로 가장 높았고, 다음으로 누리장나무 잎으로 179.26 mgGAE/g이었다.