• Korean Journal of Plant Resources
• /
• v.33 no.2
• /
• pp.86-92
• /
• 2020

This study was conducted to analyze ripening characteristics of the seed according to days after flowering. The seeds were harvested on land located in the Department of Herbal Crop Research in Rural Development Administration in 2019. Seed weight and germination rate were investigated according to days after flowering and the embryo:seed ratio was examined during the fruiting process. The results showed that the weight increased significantly by the days after flowering at each inflorescence and the seed began to germinate at different time. Further, given the embryo:seed ratio, we found that embryo continue to grow in the seed. Because Angelica acutiloba (Siebold & Zucc.) Kitagawa bloom in various inflorescences, the stage of embryonic development of the seeds can affect the germination of seed. Based on our results, the key seed harvest period for good seed is 50 to 70 days after flowering.

• Korean Journal of Food Science and Technology
• /
• v.30 no.2
• /
• pp.456-460
• /
• 1998

This research was attempted to prove suppressing effect of medicinal plants on the intestinal absorption of toxic heavy metals. In vitro study was performed by membrane filtration considering intestinal absorption conditions. From drinking water contaminated singly with 10 and 50 times level of water quality standard for heavy metals, the removal ratio of lead was $40{\sim}60%$ by Chicorium intybus, Angelica acutiloba and Ganoderma lucidium. And the removal ratio of cadmium was $20{\sim}40%$. The removal ratio of lead contaminated with both was $30{\sim}50%$ by every sample similarly, the removal ratio of cadmium was $10{\sim}30%$. The removal ratio of Angelica acutiloba was more higher than Chicorium intybus and Ganoderma lucidium. Considering the extraction conditions of samples, $70^{\circ}C$, 2 min conditions were higher than $95^{\circ}C$, 10 min conditions, the removal ratios of heavy metals were similar. And the membrane filter permeated the material under 500 mw selectively, so the final filtrate could regard as passive transport in intestinal absorption. In conclusion, this research exhibited that the medicinal plants beverages have a suppressing effect on intestinal absorption of lead and cadmium in drinking water.

• Korean Journal of Pharmacognosy
• /
• v.30 no.1
• /
• pp.59-64
• /
• 1999

Supercritical fluid extraction (SFE) technique was applied to extract cytotoxic substances from five medicinal plants including Angelica gigas, Angelica acutiloba, Aralia cordata, Spirodela polyrhiza, Bupleurum falcatum, and Acanthopanax sessiliflorus. The cytotoxicities against P388, A549, and HL-60 cell lines were determined for the supercritical carbon dioxide extracts of five plant materials employed and were compared with those of the conventional organic solvent extracts such as n-hexane, $CHCl_{3}$, and MeOH to evaluate the SFE as an alternative method to conventional organic solvent extraction. In most cases, the SFE extracts of plant materials showed enhanced cytotoxicities when compared with those of other organic solvent extracts. In addition, the optimum temperature and pressure of SFE for extraction of the cytotoxic substances were largely affected by both the plant species and the cell lines tested. These results suggested that SFE could be an alternative to the conventional organic solvent method for the selective extraction of cytotoxic compounds from plants.

• Korean Journal of Environmental Agriculture
• /
• v.24 no.1
• /
• pp.34-39
• /
• 2005

To investigate the germanium content in paddy soil in Gyeongnam province, 310 paddy soil samples were collected at 19 areas in Gyeongnam, Ulsan and Busan. Contents of germanium in paddy soils were analyzed in different topographies, soil types and soil textures. Average content of germanium in Gyeongnam was 0.24 mr/kg, those of Masan, Jinju and Hadong were above 0.30 mg/kg. Germanium content with different topographies were no difference. Germanium contents in different soil types were 0.27 mg/kg in well adapted soil and 0.23 mg/kg in poorly drained soil. Germanium contents in different soil textures were 0.27 mg/kg in silt loam and 0.23 mg/kg in sandy loam. To determine germanium content on agricultural product in the field, content of germanium in cereals, vegetables and fruits were analyzed. Germanium content of agricultural product was high in the order of cereals>vegetables>fruits. In case of vegetables, germanium contents were high in the order of leaf vegetables>root vegetables>fruit vegetables. Germanium contents were high with 62 and $65{\mu}g/kg$ in lettuce and young radish, respectively. To analyze the germanium content in medicinal plant, samples were collected from 19 medicinal plants at Hamyang areas. Germanium contents in Angelica keiskei, Ligusticum chuanxiong, Panax ginseng and Atractylodes macrocephala were relatively high with $100{\mu}g/kg$ above.

• Korean Journal of Plant Resources
• /
• v.8 no.3
• /
• pp.281-286
• /
• 1995

In order to get basic information for utilization of yong leabes of medicinal herbs as vegetable, 7 species leaves of medicinal herbs were analyzed. The moisture content in 6 species leaves were 79.74 to 85.97%. The raw protein content were about 2% except Angelica gigas (1.12%) and Peucedanum japonica (1.27%). The raw fat were 0.96 to 1.36%, the raw fiber were 1.8 to 3.9% and the raw ash were 1.58 to 2.79%. There content of medicinal herbs were similar to general vegetables. The K contents were the highest among the medical components in all samples. The total contents of amino acid in Aralza elata was 965mg%, Scutellaria baicalensis 930mg%, Aralia cordata 879mg%, Angelica acutiloba 851mg%, Agastache rugosa 747mg%, and Peucedanum japonica 615mg%, Aspartic acid and glutamic acid in amino acid were generally the highest but methionine and cystine the lowest.

• Korean Journal of Pharmacognosy
• /
• v.33 no.1 s.128
• /
• pp.35-41
• /
• 2002

The MeOH ext., $CH_2Cl_2$ Frac., EtOAc Frac., n-BuOH Frac., and $H_2O$ Frac. of 23 Korean medicinal herbs were prepared and were tested the inhibitory effects on Cytochrome P450 (Cyp) 1A1/2, 2B1/2, 2E1. Among the tested samples, the extracts of Selaginella tamariscina, Euonymus alatus, Salvia miltiorhiza, Angelica acutiloba, Rheum palmatum, Paeonia moutan, Scutellaria barbata, Tribulus terrestris, Hedyotis diffusa, Curcuma zedoaria, Rehmania glutinosa, Trogopterus xanthipes, Melandryum firmum, Achyranthes bidentata, Leonurus sibricus, Panax ginseng, Paeonia lactiflora, Poncirus trifoliata, Cnidium officinale, Cyperus rotundus, Corydalis ternata showed significant inhibitory effects on Cyp 1A1/2, 2B1/2, 2E1. The $IC_{50}$ values of those extracts were found to be below $50\;{\mu}g/ml$.

• Korean Journal of Pharmacognosy
• /
• v.35 no.1 s.136
• /
• pp.28-34
• /
• 2004

DNA damage induced by reactive oxygen species (ROS) seems to play an important role in the induction of mutation and cancer. Hydrogen peroxide $(H_2O_2)$ has been shown to induce a variety of genetic alterations, probably by the generation of hydroxyl radicals via Fenton reaction. In this study, we examined the ability of medicinal herbs in the suppression of $H_2O_2$-induced mutagenesis. Human fibroblast GM00637 cells were treated with $H_2O_2$ in the presence or absence of medicinal herbs, and $H_2O_2$-induced mutant frequency was measured at the hypoxanthine guanine phosphoribosyl transferase (HPRT) locus. Treatment of cells with various doses of $H_2O_2$ caused a significant increase of the HPRT mutant frequency. However, pretreatment of cells with several medicinal herbs reduced $H_2O_2$-induced mutant frequency. The strong antimutagenic effects were observed from the methylene chloride and ethyl acetate fractions of Selaginella tamariscina, Panax ginseng, and Angelica acutiloba; ethyl acetate fractions of Rehmania glutinosa, Leonurus sibiricus, Curcuma zedoaria and Commiphora molmol; butanol fractions of Scutellaria barbata, Tribulus terrestris, Curcuma zedoaria, Cyperus rotundus and Carthamus tinctorius, which were more than 60% inhibition of $H_2O_2$-induced mutant frequency at the HPRT locus.

• Journal of Digital Convergence
• /
• v.15 no.11
• /
• pp.513-522
• /
• 2017

We tried to analyze the inflammation reactions by treatment of AG, AS and AA in murine RAW 264.7 cells. To investigate the effect of AG, AS and AA on cell viability of RAW 264.7 cells, AG, AS and AA were treated for 24 h and MTS assay was performed. Cell viabilities were increased in $1,600{\mu}g/ml$ concentration by AS, AA and AG treatments, respectively. The mRNA expression levels of $IL-1{\beta}$, $TNF-{\alpha}$ and iNOS were increased by AG and AA treatment at a concentration of $200{\mu}g/ml$ in RAW 264.7 cells without Lipopolysaccharide (LPS) treatment. The mRNA expression levels of $IL-1{\beta}$, $TNF-{\alpha}$ and iNOS were increased by AG and AA 6 h treatment at a concentration of $200{\mu}g/ml$ with LPS treatment. In this study, we observed that AG, AS and AA show various activities on inflammation reaction depend on their treatment time. In the future, studies should be conducted to investigate the effects of AG, AS and AA on the various inflammatory responses of macrophages.

• The Korea Journal of Herbology
• /
• v.26 no.4
• /
• pp.67-74
• /
• 2011

Objectives : The purpose of the study is to determine the neuroprotective effects of the water extract of Angelicae Acutilobae Radix(AA) on ischemia reperfusion-induced apoptosis in SK-N-SH human brain neuronal cells. Methods: SK-N-SH cells were treated with different concentrations of AA water extract (0.1, 0.2, 0.5 and 1.0 mg/ml) for 2 hr and then stimulated with Dulbecco's phosphate-buffered saline containing CI-DPBS: 3mM sodium azide and 10 mM 2-deoxy-D-glucose for 45 min, reperfused with growth medium, and incubated for 24 h. Cell viability was determined by WST-1 assay, and ATP/ADP levels were measured by ADP/ATP ratio assay kit. The levels of caspase-3 protein were determined by Western blot and apoptotic body was observed by Hoechst 33258 staining. Results : AA extract significantly inhibited decreasing the cell viability in ischemia-induced SK-N-SH cells. AA also increased the ratio of ADP/ATP in ischemia-induced neuronal cells and decreased the expression levels of apoptotic protein, caspase-3 and apoptotic DNA damage. Conclusions : Our results suggest that AA extract has a neuroprotective property via suppressing the apoptosis and increasing the energy levels in neuronal cells, suggesting that AA extract may has a therapeutic potential in the treatment of ischemic brain injury.

• Korean Journal of Food Science and Technology
• /
• v.33 no.1
• /
• pp.135-141
• /
• 2001

Of hot-water extracts prepared from 10 herbal components of Sip-Jeon-Dae-Bo-Tang, Atractylodes lancea DC. (ALR) and Panax ginseng C.A. Meyer (PG) showed the most potent bone marrow cell proliferation activity through intestinal immune system whereas other extracts did not have the activity except for Astragalus membranacues Bunge (ASR) and Angelica acutiloba Kitagawa (AR) having low activity. Especially, ALR had the potent activity irrespective of classes of ALR, a place of production and the condition of breeding. In addition, we found that hot-water extract from Atractylodes lancea DC rhizomes (ALR-0) contributed mainly to Peyer's patch cells mediated-hematopoietic response of Sip-Jeon-Dae-Bo-Tang. ALR-0 was further fractionated into MeOH-soluble fraction (ALR-1), MeOH-insoluble and EtOH-soluble fraction (ALR-2), and the crude polysaccharide fraction (ALR-3). Among these fractions, only ALR-3 showed potent stimulating activity for proliferation of bone marrow cells mediated by Peyer's patch cells, dose-dependently. In treatments of ALR-3 with $NaIO_4,\;NaClO_2$ and pronase, all significantly reduced the intestinal immune system modulating activity of ALR-3, and the activity of ALR-3 was much affected by $NaIO_4$ oxidation particularly. These results reveal that macromolecules, such as polysaccharide, rather than low-molecular-weight substances, are the potent intestinal immune system modulating compound of ALR.